DE69936147D1 - High fidelity thermostabile ligase und ihre verwendung - Google Patents

High fidelity thermostabile ligase und ihre verwendung

Info

Publication number
DE69936147D1
DE69936147D1 DE69936147T DE69936147T DE69936147D1 DE 69936147 D1 DE69936147 D1 DE 69936147D1 DE 69936147 T DE69936147 T DE 69936147T DE 69936147 T DE69936147 T DE 69936147T DE 69936147 D1 DE69936147 D1 DE 69936147D1
Authority
DE
Germany
Prior art keywords
ligase
thermostabile
high fidelity
reaction process
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69936147T
Other languages
English (en)
Other versions
DE69936147T2 (de
Inventor
Francis Barany
Weiguo Cao
Jie Tong
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cornell Research Foundation Inc
Original Assignee
Cornell Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell Research Foundation Inc filed Critical Cornell Research Foundation Inc
Publication of DE69936147D1 publication Critical patent/DE69936147D1/de
Application granted granted Critical
Publication of DE69936147T2 publication Critical patent/DE69936147T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Vessels, Lead-In Wires, Accessory Apparatuses For Cathode-Ray Tubes (AREA)
  • Confectionery (AREA)
  • Dowels (AREA)
DE69936147T 1998-10-30 1999-10-29 High fidelity thermostabile ligase und ihre verwendung Expired - Lifetime DE69936147T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10646198P 1998-10-30 1998-10-30
US106461P 1998-10-30
PCT/US1999/025437 WO2000026381A2 (en) 1998-10-30 1999-10-29 High fidelity thermostable ligase and uses thereof

Publications (2)

Publication Number Publication Date
DE69936147D1 true DE69936147D1 (de) 2007-07-05
DE69936147T2 DE69936147T2 (de) 2008-01-24

Family

ID=22311532

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69936147T Expired - Lifetime DE69936147T2 (de) 1998-10-30 1999-10-29 High fidelity thermostabile ligase und ihre verwendung

Country Status (7)

Country Link
EP (1) EP1127135B1 (de)
JP (1) JP4598275B2 (de)
AT (1) ATE362986T1 (de)
AU (1) AU776820B2 (de)
CA (1) CA2348776C (de)
DE (1) DE69936147T2 (de)
WO (1) WO2000026381A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6949370B1 (en) 1998-10-30 2005-09-27 Cornell Research Foundation, Inc. High fidelity thermostable ligase and uses thereof
AU2001253332A1 (en) * 2000-04-14 2001-10-30 Livecapital, Inc. Online credit services brokering
US20050064490A1 (en) * 2003-09-23 2005-03-24 University Of Missouri Methods of synthesizing polynucleotides using thermostable enzymes
CA3049989A1 (en) 2017-02-10 2018-08-16 Zymergen Inc. A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts
JP2021533773A (ja) 2018-08-15 2021-12-09 ザイマージェン インコーポレイテッド ハイスループット代謝操作におけるＣＲＩＳＰＲｉの適用
WO2022015461A1 (en) 2020-07-17 2022-01-20 Qiagen Beverly Llc Thermostable ligase with reduced sequence bias
CN117247911B (zh) * 2023-08-22 2024-06-07 武汉爱博泰克生物科技有限公司 大肠杆菌dna连接酶突变体及其在毕赤酵母中表达纯化方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE459709T1 (de) * 1990-05-03 2010-03-15 Cornell Res Foundation Inc Hitzestabiles ligase enthaltendes dns- amplifikationssystem zur bestimmung von genetischen krankheiten
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction

Also Published As

Publication number Publication date
WO2000026381A2 (en) 2000-05-11
AU1331000A (en) 2000-05-22
JP2002528121A (ja) 2002-09-03
AU776820B2 (en) 2004-09-23
EP1127135B1 (de) 2007-05-23
CA2348776C (en) 2008-03-25
CA2348776A1 (en) 2000-05-11
WO2000026381A3 (en) 2000-11-09
DE69936147T2 (de) 2008-01-24
ATE362986T1 (de) 2007-06-15
JP4598275B2 (ja) 2010-12-15
EP1127135A2 (de) 2001-08-29

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition