DE69739356D1 - VERFAHREN ZUR KLONIERUNG VON cDNA MIT VOLLER LÄNGE - Google Patents

VERFAHREN ZUR KLONIERUNG VON cDNA MIT VOLLER LÄNGE

Info

Publication number
DE69739356D1
DE69739356D1 DE69739356T DE69739356T DE69739356D1 DE 69739356 D1 DE69739356 D1 DE 69739356D1 DE 69739356 T DE69739356 T DE 69739356T DE 69739356 T DE69739356 T DE 69739356T DE 69739356 D1 DE69739356 D1 DE 69739356D1
Authority
DE
Germany
Prior art keywords
mrna
oligonucleotide
full
cdna
length cdna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69739356T
Other languages
English (en)
Inventor
Alex Chenchik
York Zhu
Luda Diatchenko
Paul Siebert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takara Bio USA Inc
Original Assignee
Clontech Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Clontech Laboratories Inc filed Critical Clontech Laboratories Inc
Application granted granted Critical
Publication of DE69739356D1 publication Critical patent/DE69739356D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69739356T 1996-01-03 1997-01-03 VERFAHREN ZUR KLONIERUNG VON cDNA MIT VOLLER LÄNGE Expired - Lifetime DE69739356D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/582,562 US5962271A (en) 1996-01-03 1996-01-03 Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
PCT/US1997/000368 WO1997024455A2 (en) 1996-01-03 1997-01-03 METHODS AND COMPOSITIONS FOR FULL-LENGTH cDNA CLONING

Publications (1)

Publication Number Publication Date
DE69739356D1 true DE69739356D1 (de) 2009-05-28

Family

ID=24329625

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69739356T Expired - Lifetime DE69739356D1 (de) 1996-01-03 1997-01-03 VERFAHREN ZUR KLONIERUNG VON cDNA MIT VOLLER LÄNGE

Country Status (7)

Country Link
US (2) US5962271A (de)
EP (1) EP0871780B1 (de)
JP (1) JP4043516B2 (de)
AT (1) ATE428800T1 (de)
DE (1) DE69739356D1 (de)
ES (1) ES2325044T3 (de)
WO (1) WO1997024455A2 (de)

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