BR0008313A - Composições e métodos para ativação não-direcionada de genes endógenos - Google Patents
Composições e métodos para ativação não-direcionada de genes endógenosInfo
- Publication number
- BR0008313A BR0008313A BR0008313-5A BR0008313A BR0008313A BR 0008313 A BR0008313 A BR 0008313A BR 0008313 A BR0008313 A BR 0008313A BR 0008313 A BR0008313 A BR 0008313A
- Authority
- BR
- Brazil
- Prior art keywords
- gene
- genes
- methods
- expression
- directed
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/20—Pseudochromosomes, minichrosomosomes
- C12N2800/204—Pseudochromosomes, minichrosomosomes of bacterial origin, e.g. BAC
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/60—Vectors containing traps for, e.g. exons, promoters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
"COMPOSIçõES E MéTODOS PARA ATIVAçãO NãO-DIRECIONADA DE GENES ENDóGENOS". A presente invenção é dirigida de forma geral à ativação da expressão de gene ou à produção de superexpressão de um gene por métodos de recombinação in situ. A invenção também é direcionada de forma geral aos métodos para a expressão de um gene endógeno em uma célula em níveis maiores do que aqueles normalmente encontrados na célula. Em uma modalidade da invenção, a expressão de um gene endógeno é ativada ou aumentada após integração na célula, por recombinação não-homóloga ou ilegítima, de uma seq³ência reguladora que ativa a expressão do gene. Em outra modalidade, a expressão do gene endógeno pode ainda ser aumentada por co-integração de um ou mais marcadores amplificáveis, e selecionando-se cópias aumentadas de um ou mais marcadores amplificáveis localizados no vetor integrado. Em outra modalidade, a invenção é dirigida à ativação de genes endógenos por integração não direcionada de vetores de ativação não especializados, que são fornecidos pela invenção, no genoma de uma célula hospedeira. A invenção também fornece métodos para a identificação, ativação, isolamento, e/ou expressão de genes não descobertos por métodos atuais uma vez que nenhuma seq³ência alvo é necessária para integração. A invenção também fornece métodos para isolamento de moléculas de ácido nucléico (particularmente moléculas de cDNA) que codificam diversas proteínas, incluindo proteínas transmembrana, e para o isolamento de células que expressam tais proteínas transmembrana, que podem ser proteínas transmembrana heterólogas. A invenção também é dirigida a genes isolados, produtos de gene, moléculas de ácido nucléico, e a composições que compreendem tais genes, produtos de gene e moléculas de ácido nucléico, e a vetores e células hospedeiras que compreendem tais genes e moléculas de ácido nucléico, que podem ser usados em diversas aplicações terapêuticas e diagnósticas. Dessa forma, pela presente invenção, genes endógenos, incluindo aqueles associados com doenças humanas e desenvolvimento, podem ser ativados e isolados sem conhecimento prévio do perfil da seq³ência, estrutura, função, ou expressão dos genes.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US25302299A | 1999-02-19 | 1999-02-19 | |
US26381499A | 1999-03-08 | 1999-03-08 | |
US09/276,820 US6897066B1 (en) | 1997-09-26 | 1999-03-26 | Compositions and methods for non-targeted activation of endogenous genes |
PCT/US2000/004429 WO2000049162A2 (en) | 1999-02-19 | 2000-02-22 | Compositions and methods for non-targeted activation of endogenous genes |
Publications (1)
Publication Number | Publication Date |
---|---|
BR0008313A true BR0008313A (pt) | 2003-09-23 |
Family
ID=27400625
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0008313-5A BR0008313A (pt) | 1999-02-19 | 2000-02-22 | Composições e métodos para ativação não-direcionada de genes endógenos |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP1155131B1 (pt) |
JP (1) | JP2004501601A (pt) |
CN (1) | CN1384881A (pt) |
AU (1) | AU3003700A (pt) |
BR (1) | BR0008313A (pt) |
CA (1) | CA2364267A1 (pt) |
IL (1) | IL144960A0 (pt) |
WO (1) | WO2000049162A2 (pt) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ504185A (en) * | 1997-09-26 | 2003-02-28 | Athersys Inc | Expression of endogenous genes by non-homologous recombination of a vector construct with cellular DNA |
US6897066B1 (en) | 1997-09-26 | 2005-05-24 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
WO2001042442A2 (en) | 1999-12-10 | 2001-06-14 | Cytos Biotechnology Ag | Activation of endogenous genes by genomic introduction of a replicon |
US8501396B2 (en) | 2001-11-05 | 2013-08-06 | Medgenics Medical Israel Ltd. | Dermal micro-organs, methods and apparatuses for producing and using the same |
US7468242B2 (en) | 2001-11-05 | 2008-12-23 | Medgenics, Inc. | Dermal micro organs, methods and apparatuses for producing and using the same |
EP2377404A1 (en) * | 2003-05-01 | 2011-10-19 | Medgenics, Inc. | A dermal micro-organ which is an explant of living tissue |
EP1662005A1 (en) * | 2004-11-26 | 2006-05-31 | FrankGen Biotechnologie AG | Enhancer-containing gene trap vectors for random and targeted gene trapping |
EP1907561B1 (en) * | 2005-06-24 | 2013-08-28 | Unigene Laboratories, Inc. | Cell lines for expressing enzyme useful in the preparation of amidated products |
EP2525808A2 (en) | 2010-01-19 | 2012-11-28 | The Trustees Of Columbia University In The City Of New York | Osteocalcin as a treatment for male reproductive disorders |
US10052364B2 (en) | 2013-03-15 | 2018-08-21 | The Trustees Of Columbia University In The City Of New York | Osteocalcin as a treatment for cognitive disorders |
US20160046694A1 (en) * | 2013-03-30 | 2016-02-18 | Usha Biotech Limited | Methods and constructs for expressing biologically active proteins in mammalian cells |
JP6784601B2 (ja) * | 2014-06-23 | 2020-11-11 | ザ ジェネラル ホスピタル コーポレイション | シークエンシングによって評価されるゲノムワイドでバイアスのないDSBの同定(GUIDE−Seq) |
EP3226889A4 (en) | 2014-11-19 | 2018-11-21 | The Trustees of Columbia University in the City of New York | Osteocalcin as a treatment for frailty associated with aging |
EP3448981B1 (en) * | 2016-04-30 | 2022-12-07 | Biolegend, Inc. | Compositions and methods for performing magnetibuoyant separations |
CN111593037B (zh) * | 2020-06-15 | 2021-02-09 | 南通大学 | 一种高效的爬行动物来源的凝血酶及其应用 |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2128227C1 (ru) * | 1989-12-22 | 1999-03-27 | Апплайд Резеч Системз АРС Холдинг Н.В. (NL) (Антильские острова) | Способ активации транскрипционно-молчащего гена |
US6270989B1 (en) * | 1991-11-05 | 2001-08-07 | Transkaryotic Therapies, Inc. | Protein production and delivery |
US5641670A (en) * | 1991-11-05 | 1997-06-24 | Transkaryotic Therapies, Inc. | Protein production and protein delivery |
WO1993018176A1 (en) * | 1992-03-11 | 1993-09-16 | Dana-Farber Cancer Institute | METHODS TO CLONE mRNA |
TW402639B (en) * | 1992-12-03 | 2000-08-21 | Transkaryotic Therapies Inc | Protein production and protein delivery |
JPH09173100A (ja) * | 1995-10-27 | 1997-07-08 | Sapporo Breweries Ltd | ホップ潜在ウイルスの遺伝子及び該ウイルスの検出方法 |
US5962271A (en) * | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
JP2001503962A (ja) * | 1996-02-09 | 2001-03-27 | ヘット ネーデルランツ カンケル インスティチュート | 細胞のゲノム中に組込まれる付加核酸物質を細胞に付与するためのベクターおよび方法 |
AU4648597A (en) * | 1996-09-23 | 1998-04-14 | Chiron Corporation | Random and targeted promoter integration for gene analysis |
US6139833A (en) * | 1997-08-08 | 2000-10-31 | Lexicon Genetics Incorporated | Targeted gene discovery |
JP2002511741A (ja) * | 1997-03-11 | 2002-04-16 | リージェンツ オブ ザ ユニバーシティ オブ ミネソタ | 細胞のdnaに核酸を導入するためのdna系トランスポゾンシステム |
WO1999005527A2 (en) * | 1997-07-22 | 1999-02-04 | Novimmune Sa | Methods for diagnosis and therapy of autoimmune disease, such as insulin dependent diabetes mellitus, involving retroviral superantigens |
AU8752898A (en) * | 1997-08-12 | 1999-03-01 | Het Nederlands Kanker Instituut | Vectors and methods for providing cells with additional nucleic acid materi al integrated in the genome of said cells |
NZ504185A (en) * | 1997-09-26 | 2003-02-28 | Athersys Inc | Expression of endogenous genes by non-homologous recombination of a vector construct with cellular DNA |
EP1066392B1 (en) * | 1998-03-27 | 2008-07-09 | Lexicon Pharmaceuticals, Inc. | Vectors for gene mutagenesis and gene discovery |
US6436707B1 (en) * | 1998-03-27 | 2002-08-20 | Lexicon Genetics Incorporated | Vectors for gene mutagenesis and gene discovery |
-
2000
- 2000-02-22 AU AU30037/00A patent/AU3003700A/en not_active Withdrawn
- 2000-02-22 JP JP2000599886A patent/JP2004501601A/ja active Pending
- 2000-02-22 CA CA002364267A patent/CA2364267A1/en not_active Abandoned
- 2000-02-22 WO PCT/US2000/004429 patent/WO2000049162A2/en not_active Application Discontinuation
- 2000-02-22 IL IL14496000A patent/IL144960A0/xx unknown
- 2000-02-22 CN CN00806377.XA patent/CN1384881A/zh active Pending
- 2000-02-22 EP EP00908750A patent/EP1155131B1/en not_active Expired - Lifetime
- 2000-02-22 BR BR0008313-5A patent/BR0008313A/pt not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
IL144960A0 (en) | 2002-06-30 |
CA2364267A1 (en) | 2000-08-24 |
AU3003700A (en) | 2000-09-04 |
WO2000049162A3 (en) | 2000-12-28 |
WO2000049162A2 (en) | 2000-08-24 |
CN1384881A (zh) | 2002-12-11 |
EP1155131A2 (en) | 2001-11-21 |
EP1155131B1 (en) | 2008-07-23 |
WO2000049162A8 (en) | 2001-03-01 |
JP2004501601A (ja) | 2004-01-22 |
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Legal Events
Date | Code | Title | Description |
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B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 4A, 5A, 6A, 7A E 8A ANUIDADES. |
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B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1929 DE 26/12/2007. |