BR0008313A - Composições e métodos para ativação não-direcionada de genes endógenos - Google Patents

Composições e métodos para ativação não-direcionada de genes endógenos

Info

Publication number
BR0008313A
BR0008313A BR0008313-5A BR0008313A BR0008313A BR 0008313 A BR0008313 A BR 0008313A BR 0008313 A BR0008313 A BR 0008313A BR 0008313 A BR0008313 A BR 0008313A
Authority
BR
Brazil
Prior art keywords
gene
genes
methods
expression
directed
Prior art date
Application number
BR0008313-5A
Other languages
English (en)
Inventor
John J Harrington
Bruce Sherf
Stephen Rundlett
Original Assignee
Athersys Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/276,820 external-priority patent/US6897066B1/en
Application filed by Athersys Inc filed Critical Athersys Inc
Publication of BR0008313A publication Critical patent/BR0008313A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/204—Pseudochromosomes, minichrosomosomes of bacterial origin, e.g. BAC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/60—Vectors containing traps for, e.g. exons, promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

"COMPOSIçõES E MéTODOS PARA ATIVAçãO NãO-DIRECIONADA DE GENES ENDóGENOS". A presente invenção é dirigida de forma geral à ativação da expressão de gene ou à produção de superexpressão de um gene por métodos de recombinação in situ. A invenção também é direcionada de forma geral aos métodos para a expressão de um gene endógeno em uma célula em níveis maiores do que aqueles normalmente encontrados na célula. Em uma modalidade da invenção, a expressão de um gene endógeno é ativada ou aumentada após integração na célula, por recombinação não-homóloga ou ilegítima, de uma seq³ência reguladora que ativa a expressão do gene. Em outra modalidade, a expressão do gene endógeno pode ainda ser aumentada por co-integração de um ou mais marcadores amplificáveis, e selecionando-se cópias aumentadas de um ou mais marcadores amplificáveis localizados no vetor integrado. Em outra modalidade, a invenção é dirigida à ativação de genes endógenos por integração não direcionada de vetores de ativação não especializados, que são fornecidos pela invenção, no genoma de uma célula hospedeira. A invenção também fornece métodos para a identificação, ativação, isolamento, e/ou expressão de genes não descobertos por métodos atuais uma vez que nenhuma seq³ência alvo é necessária para integração. A invenção também fornece métodos para isolamento de moléculas de ácido nucléico (particularmente moléculas de cDNA) que codificam diversas proteínas, incluindo proteínas transmembrana, e para o isolamento de células que expressam tais proteínas transmembrana, que podem ser proteínas transmembrana heterólogas. A invenção também é dirigida a genes isolados, produtos de gene, moléculas de ácido nucléico, e a composições que compreendem tais genes, produtos de gene e moléculas de ácido nucléico, e a vetores e células hospedeiras que compreendem tais genes e moléculas de ácido nucléico, que podem ser usados em diversas aplicações terapêuticas e diagnósticas. Dessa forma, pela presente invenção, genes endógenos, incluindo aqueles associados com doenças humanas e desenvolvimento, podem ser ativados e isolados sem conhecimento prévio do perfil da seq³ência, estrutura, função, ou expressão dos genes.
BR0008313-5A 1999-02-19 2000-02-22 Composições e métodos para ativação não-direcionada de genes endógenos BR0008313A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US25302299A 1999-02-19 1999-02-19
US26381499A 1999-03-08 1999-03-08
US09/276,820 US6897066B1 (en) 1997-09-26 1999-03-26 Compositions and methods for non-targeted activation of endogenous genes
PCT/US2000/004429 WO2000049162A2 (en) 1999-02-19 2000-02-22 Compositions and methods for non-targeted activation of endogenous genes

Publications (1)

Publication Number Publication Date
BR0008313A true BR0008313A (pt) 2003-09-23

Family

ID=27400625

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0008313-5A BR0008313A (pt) 1999-02-19 2000-02-22 Composições e métodos para ativação não-direcionada de genes endógenos

Country Status (8)

Country Link
EP (1) EP1155131B1 (pt)
JP (1) JP2004501601A (pt)
CN (1) CN1384881A (pt)
AU (1) AU3003700A (pt)
BR (1) BR0008313A (pt)
CA (1) CA2364267A1 (pt)
IL (1) IL144960A0 (pt)
WO (1) WO2000049162A2 (pt)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ504185A (en) * 1997-09-26 2003-02-28 Athersys Inc Expression of endogenous genes by non-homologous recombination of a vector construct with cellular DNA
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
WO2001042442A2 (en) 1999-12-10 2001-06-14 Cytos Biotechnology Ag Activation of endogenous genes by genomic introduction of a replicon
US8501396B2 (en) 2001-11-05 2013-08-06 Medgenics Medical Israel Ltd. Dermal micro-organs, methods and apparatuses for producing and using the same
US7468242B2 (en) 2001-11-05 2008-12-23 Medgenics, Inc. Dermal micro organs, methods and apparatuses for producing and using the same
EP2377404A1 (en) * 2003-05-01 2011-10-19 Medgenics, Inc. A dermal micro-organ which is an explant of living tissue
EP1662005A1 (en) * 2004-11-26 2006-05-31 FrankGen Biotechnologie AG Enhancer-containing gene trap vectors for random and targeted gene trapping
EP1907561B1 (en) * 2005-06-24 2013-08-28 Unigene Laboratories, Inc. Cell lines for expressing enzyme useful in the preparation of amidated products
EP2525808A2 (en) 2010-01-19 2012-11-28 The Trustees Of Columbia University In The City Of New York Osteocalcin as a treatment for male reproductive disorders
US10052364B2 (en) 2013-03-15 2018-08-21 The Trustees Of Columbia University In The City Of New York Osteocalcin as a treatment for cognitive disorders
US20160046694A1 (en) * 2013-03-30 2016-02-18 Usha Biotech Limited Methods and constructs for expressing biologically active proteins in mammalian cells
JP6784601B2 (ja) * 2014-06-23 2020-11-11 ザ ジェネラル ホスピタル コーポレイション シークエンシングによって評価されるゲノムワイドでバイアスのないＤＳＢの同定（ＧＵＩＤＥ−Ｓｅｑ）
EP3226889A4 (en) 2014-11-19 2018-11-21 The Trustees of Columbia University in the City of New York Osteocalcin as a treatment for frailty associated with aging
EP3448981B1 (en) * 2016-04-30 2022-12-07 Biolegend, Inc. Compositions and methods for performing magnetibuoyant separations
CN111593037B (zh) * 2020-06-15 2021-02-09 南通大学 一种高效的爬行动物来源的凝血酶及其应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2128227C1 (ru) * 1989-12-22 1999-03-27 Апплайд Резеч Системз АРС Холдинг Н.В. (NL) (Антильские острова) Способ активации транскрипционно-молчащего гена
US6270989B1 (en) * 1991-11-05 2001-08-07 Transkaryotic Therapies, Inc. Protein production and delivery
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
WO1993018176A1 (en) * 1992-03-11 1993-09-16 Dana-Farber Cancer Institute METHODS TO CLONE mRNA
TW402639B (en) * 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
JPH09173100A (ja) * 1995-10-27 1997-07-08 Sapporo Breweries Ltd ホップ潜在ウイルスの遺伝子及び該ウイルスの検出方法
US5962271A (en) * 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
JP2001503962A (ja) * 1996-02-09 2001-03-27 ヘット ネーデルランツ カンケル インスティチュート 細胞のゲノム中に組込まれる付加核酸物質を細胞に付与するためのベクターおよび方法
AU4648597A (en) * 1996-09-23 1998-04-14 Chiron Corporation Random and targeted promoter integration for gene analysis
US6139833A (en) * 1997-08-08 2000-10-31 Lexicon Genetics Incorporated Targeted gene discovery
JP2002511741A (ja) * 1997-03-11 2002-04-16 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ 細胞のｄｎａに核酸を導入するためのｄｎａ系トランスポゾンシステム
WO1999005527A2 (en) * 1997-07-22 1999-02-04 Novimmune Sa Methods for diagnosis and therapy of autoimmune disease, such as insulin dependent diabetes mellitus, involving retroviral superantigens
AU8752898A (en) * 1997-08-12 1999-03-01 Het Nederlands Kanker Instituut Vectors and methods for providing cells with additional nucleic acid materi al integrated in the genome of said cells
NZ504185A (en) * 1997-09-26 2003-02-28 Athersys Inc Expression of endogenous genes by non-homologous recombination of a vector construct with cellular DNA
EP1066392B1 (en) * 1998-03-27 2008-07-09 Lexicon Pharmaceuticals, Inc. Vectors for gene mutagenesis and gene discovery
US6436707B1 (en) * 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery

Also Published As

Publication number Publication date
IL144960A0 (en) 2002-06-30
CA2364267A1 (en) 2000-08-24
AU3003700A (en) 2000-09-04
WO2000049162A3 (en) 2000-12-28
WO2000049162A2 (en) 2000-08-24
CN1384881A (zh) 2002-12-11
EP1155131A2 (en) 2001-11-21
EP1155131B1 (en) 2008-07-23
WO2000049162A8 (en) 2001-03-01
JP2004501601A (ja) 2004-01-22

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

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