ATE428800T1 - Verfahren zur klonierung von cdna mit voller länge - Google Patents

Verfahren zur klonierung von cdna mit voller länge

Info

Publication number
ATE428800T1
ATE428800T1 AT97902881T AT97902881T ATE428800T1 AT E428800 T1 ATE428800 T1 AT E428800T1 AT 97902881 T AT97902881 T AT 97902881T AT 97902881 T AT97902881 T AT 97902881T AT E428800 T1 ATE428800 T1 AT E428800T1
Authority
AT
Austria
Prior art keywords
mrna
oligonucleotide
full
cdna
length cdna
Prior art date
Application number
AT97902881T
Other languages
English (en)
Inventor
Alex Chenchik
York Zhu
Luda Diatchenko
Paul Siebert
Original Assignee
Clontech Lab Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Clontech Lab Inc filed Critical Clontech Lab Inc
Application granted granted Critical
Publication of ATE428800T1 publication Critical patent/ATE428800T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT97902881T 1996-01-03 1997-01-03 Verfahren zur klonierung von cdna mit voller länge ATE428800T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/582,562 US5962271A (en) 1996-01-03 1996-01-03 Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
PCT/US1997/000368 WO1997024455A2 (en) 1996-01-03 1997-01-03 METHODS AND COMPOSITIONS FOR FULL-LENGTH cDNA CLONING

Publications (1)

Publication Number Publication Date
ATE428800T1 true ATE428800T1 (de) 2009-05-15

Family

ID=24329625

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97902881T ATE428800T1 (de) 1996-01-03 1997-01-03 Verfahren zur klonierung von cdna mit voller länge

Country Status (7)

Country Link
US (2) US5962271A (de)
EP (1) EP0871780B1 (de)
JP (1) JP4043516B2 (de)
AT (1) ATE428800T1 (de)
DE (1) DE69739356D1 (de)
ES (1) ES2325044T3 (de)
WO (1) WO1997024455A2 (de)

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