DE69838911D1 - In-vitro Verfahren zur Bereitstellung von Fragmenten für die DNS Sequenzierung - Google Patents

In-vitro Verfahren zur Bereitstellung von Fragmenten für die DNS Sequenzierung

Info

Publication number
DE69838911D1
DE69838911D1 DE69838911T DE69838911T DE69838911D1 DE 69838911 D1 DE69838911 D1 DE 69838911D1 DE 69838911 T DE69838911 T DE 69838911T DE 69838911 T DE69838911 T DE 69838911T DE 69838911 D1 DE69838911 D1 DE 69838911D1
Authority
DE
Germany
Prior art keywords
dna
reaction
vitro method
dna sequencing
transposon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69838911T
Other languages
English (en)
Inventor
Tuomas Tenkanen
Timo Soininen
Harri Savilahti
Kirsi Multanen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thermo Fisher Scientific Oy
Original Assignee
Finnzymes Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Finnzymes Oy filed Critical Finnzymes Oy
Application granted granted Critical
Publication of DE69838911D1 publication Critical patent/DE69838911D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69838911T 1997-07-14 1998-07-10 In-vitro Verfahren zur Bereitstellung von Fragmenten für die DNS Sequenzierung Expired - Lifetime DE69838911D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI972992A FI103809B1 (fi) 1997-07-14 1997-07-14 In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
PCT/FI1998/000586 WO1999004035A1 (en) 1997-07-14 1998-07-10 In vitro method for providing templates for dna sequencing

Publications (1)

Publication Number Publication Date
DE69838911D1 true DE69838911D1 (de) 2008-02-07

Family

ID=8549258

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69838911T Expired - Lifetime DE69838911D1 (de) 1997-07-14 1998-07-10 In-vitro Verfahren zur Bereitstellung von Fragmenten für die DNS Sequenzierung

Country Status (9)

Country Link
US (1) US6593113B1 (de)
EP (1) EP1005571B1 (de)
JP (1) JP2001510055A (de)
AT (1) ATE382095T1 (de)
AU (1) AU738345B2 (de)
CA (1) CA2295968C (de)
DE (1) DE69838911D1 (de)
FI (1) FI103809B1 (de)
WO (1) WO1999004035A1 (de)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7727744B2 (en) * 2004-03-30 2010-06-01 Epicentre Technologies Corporation Methods for obtaining directionally truncated polypeptides
CA2693979A1 (en) 2007-07-26 2009-02-05 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
EP2787565B1 (de) * 2008-10-24 2015-09-16 Epicentre Technologies Corporation Transposonendenzusammensetzungen und Verfahren zur Modifizierung von Nukleinsäuren
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2012061832A1 (en) * 2010-11-05 2012-05-10 Illumina, Inc. Linking sequence reads using paired code tags
AU2012211081B2 (en) 2011-01-28 2016-02-04 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
CA2826131C (en) 2011-02-02 2019-11-05 Jay Ashok Shendure Massively parallel continguity mapping
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US9145623B2 (en) 2011-07-20 2015-09-29 Thermo Fisher Scientific Oy Transposon nucleic acids comprising a calibration sequence for DNA sequencing
US20140155295A1 (en) 2012-08-14 2014-06-05 10X Technologies, Inc. Capsule array devices and methods of use
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (de) 2012-12-14 2019-11-13 10X Genomics, Inc. Verfahren und systeme zur verarbeitung von polynukleotiden
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
ES2724824T3 (es) 2013-03-13 2019-09-16 Illumina Inc Métodos para la secuenciación de ácidos nucleicos
CN105339503B (zh) 2013-05-23 2020-04-10 斯坦福大学托管董事会 用于个人表观基因组学的至天然染色质的转座
DK3083994T3 (da) 2013-12-20 2021-09-13 Illumina Inc Bevarelse af genomisk konnektivitetsinformation i fragmenterede genomiske DNA-prøver
WO2015113725A1 (en) 2014-02-03 2015-08-06 Thermo Fisher Scientific Baltics Uab Method for controlled dna fragmentation
US11136576B2 (en) 2014-02-03 2021-10-05 Thermo Fisher Scientific Baltics Uab Method for controlled DNA fragmentation
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
JP6808617B2 (ja) 2014-10-17 2021-01-06 イルミナ ケンブリッジ リミテッド 連続性を維持した転位
CN114807307A (zh) 2014-10-29 2022-07-29 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
ES2947757T3 (es) * 2014-11-05 2023-08-18 Illumina Inc Composiciones de transposasas para reducir el sesgo de inserción
CN107427808B (zh) 2015-01-12 2020-10-23 10X基因组学有限公司 用于制备核酸测序文库的方法和系统以及用其制备的文库
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
WO2016138148A1 (en) 2015-02-24 2016-09-01 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
CN107849600A (zh) 2015-06-09 2018-03-27 生命技术公司 用于分子标记的方法、系统、组合物、试剂盒、装置和计算机可读媒体
ES2926495T3 (es) 2015-12-04 2022-10-26 10X Genomics Inc Métodos y composiciones para el análisis de ácidos nucleicos
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4095263A1 (de) 2017-01-06 2022-11-30 Editas Medicine, Inc. Verfahren zur beurteilung der nukleasespaltung
CN117512066A (zh) 2017-01-30 2024-02-06 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
EP3652312A1 (de) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systeme und verfahren zur gezielten integration und genomeditierung und deren detektion unter verwendung von integrierten bindungsstellen
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3746554A1 (de) 2018-01-30 2020-12-09 Editas Medicine, Inc. Systeme und verfahren zur modulierung chromosomaler neuanordnungen
EP3775271A1 (de) 2018-04-06 2021-02-17 10X Genomics, Inc. Systeme und verfahren zur qualitätskontrolle in einer einzelzellenverarbeitung
WO2023225095A1 (en) 2022-05-18 2023-11-23 Illumina Cambridge Limited Preparation of size-controlled nucleic acid fragments

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons

Also Published As

Publication number Publication date
FI972992A (fi) 1999-01-15
CA2295968C (en) 2008-11-25
WO1999004035A1 (en) 1999-01-28
ATE382095T1 (de) 2008-01-15
CA2295968A1 (en) 1999-01-28
US6593113B1 (en) 2003-07-15
FI103809B (fi) 1999-09-30
AU738345B2 (en) 2001-09-13
JP2001510055A (ja) 2001-07-31
FI972992A0 (fi) 1997-07-14
EP1005571B1 (de) 2007-12-26
AU8442998A (en) 1999-02-10
FI103809B1 (fi) 1999-09-30
EP1005571A1 (de) 2000-06-07

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