DE69733366T2 - Verfahren und vorrichtungen zur unterteilung biologischer probenflüssigkeiten in mikroflüssigkeitsmengen - Google Patents
Verfahren und vorrichtungen zur unterteilung biologischer probenflüssigkeiten in mikroflüssigkeitsmengen Download PDFInfo
- Publication number
- DE69733366T2 DE69733366T2 DE69733366T DE69733366T DE69733366T2 DE 69733366 T2 DE69733366 T2 DE 69733366T2 DE 69733366 T DE69733366 T DE 69733366T DE 69733366 T DE69733366 T DE 69733366T DE 69733366 T2 DE69733366 T2 DE 69733366T2
- Authority
- DE
- Germany
- Prior art keywords
- zones
- liquid
- evaluation
- indicator
- retaining
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000007788 liquid Substances 0.000 title claims description 71
- 238000000034 method Methods 0.000 title claims description 59
- 239000012472 biological sample Substances 0.000 title description 6
- 238000011156 evaluation Methods 0.000 claims description 59
- 244000005700 microbiome Species 0.000 claims description 48
- 230000002209 hydrophobic effect Effects 0.000 claims description 40
- 239000000126 substance Substances 0.000 claims description 30
- 235000015097 nutrients Nutrition 0.000 claims description 19
- 239000003153 chemical reaction reagent Substances 0.000 claims description 18
- 238000004458 analytical method Methods 0.000 claims description 17
- 239000002250 absorbent Substances 0.000 claims description 15
- 230000002745 absorbent Effects 0.000 claims description 15
- 230000014759 maintenance of location Effects 0.000 claims description 11
- 239000011248 coating agent Substances 0.000 claims description 8
- 238000000576 coating method Methods 0.000 claims description 8
- 239000003269 fluorescent indicator Substances 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 73
- 238000012360 testing method Methods 0.000 description 57
- 239000000463 material Substances 0.000 description 21
- 239000002609 medium Substances 0.000 description 20
- -1 polyethylene Polymers 0.000 description 18
- 238000010790 dilution Methods 0.000 description 15
- 239000012895 dilution Substances 0.000 description 15
- 239000002120 nanofilm Substances 0.000 description 15
- 241000588724 Escherichia coli Species 0.000 description 14
- 238000011081 inoculation Methods 0.000 description 14
- 238000011534 incubation Methods 0.000 description 13
- 229920001296 polysiloxane Polymers 0.000 description 13
- 239000000758 substrate Substances 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 239000000243 solution Substances 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 11
- 239000000835 fiber Substances 0.000 description 11
- 229920001817 Agar Polymers 0.000 description 10
- 239000008272 agar Substances 0.000 description 10
- 239000004698 Polyethylene Substances 0.000 description 9
- 238000001514 detection method Methods 0.000 description 9
- 229920000573 polyethylene Polymers 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- 238000009826 distribution Methods 0.000 description 8
- 229920001721 polyimide Polymers 0.000 description 8
- 239000000080 wetting agent Substances 0.000 description 8
- 239000004743 Polypropylene Substances 0.000 description 7
- 229920001155 polypropylene Polymers 0.000 description 7
- 238000011109 contamination Methods 0.000 description 6
- 239000003349 gelling agent Substances 0.000 description 6
- 239000004642 Polyimide Substances 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 230000012010 growth Effects 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000000049 pigment Substances 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- YUDPTGPSBJVHCN-DZQJYWQESA-N 4-methylumbelliferyl beta-D-galactoside Chemical compound C1=CC=2C(C)=CC(=O)OC=2C=C1O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O YUDPTGPSBJVHCN-DZQJYWQESA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000004820 Pressure-sensitive adhesive Substances 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000012864 cross contamination Methods 0.000 description 4
- 238000004049 embossing Methods 0.000 description 4
- 238000001125 extrusion Methods 0.000 description 4
- 239000002657 fibrous material Substances 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 4
- 239000010931 gold Substances 0.000 description 4
- 229910052737 gold Inorganic materials 0.000 description 4
- 230000005661 hydrophobic surface Effects 0.000 description 4
- 239000011550 stock solution Substances 0.000 description 4
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 229910000831 Steel Inorganic materials 0.000 description 3
- 229920004895 Triton X-35 Polymers 0.000 description 3
- 238000000151 deposition Methods 0.000 description 3
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000010959 steel Substances 0.000 description 3
- 239000001974 tryptic soy broth Substances 0.000 description 3
- NNVNAJGCZFCILW-UHFFFAOYSA-N 1'-methylspiro[3,4-dihydrochromene-2,4'-piperidine]-4-amine;hydrochloride Chemical compound Cl.C1CN(C)CCC21OC1=CC=CC=C1C(N)C2 NNVNAJGCZFCILW-UHFFFAOYSA-N 0.000 description 2
- ARQXEQLMMNGFDU-JHZZJYKESA-N 4-methylumbelliferone beta-D-glucuronide Chemical compound C1=CC=2C(C)=CC(=O)OC=2C=C1O[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O ARQXEQLMMNGFDU-JHZZJYKESA-N 0.000 description 2
- 108010023063 Bacto-peptone Proteins 0.000 description 2
- 238000009631 Broth culture Methods 0.000 description 2
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 210000000941 bile Anatomy 0.000 description 2
- 239000003833 bile salt Substances 0.000 description 2
- 229940093761 bile salts Drugs 0.000 description 2
- 239000013060 biological fluid Substances 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000002872 contrast media Substances 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 230000008021 deposition Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 239000012470 diluted sample Substances 0.000 description 2
- 238000005553 drilling Methods 0.000 description 2
- 238000005530 etching Methods 0.000 description 2
- 239000004811 fluoropolymer Substances 0.000 description 2
- 229920002313 fluoropolymer Polymers 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000000608 laser ablation Methods 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000012860 organic pigment Substances 0.000 description 2
- 230000005789 organism growth Effects 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 108010050327 trypticase-soy broth Proteins 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- BCHIXGBGRHLSBE-UHFFFAOYSA-N (4-methyl-2-oxochromen-7-yl) dihydrogen phosphate Chemical compound C1=C(OP(O)(O)=O)C=CC2=C1OC(=O)C=C2C BCHIXGBGRHLSBE-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- RIWRBSMFKVOJMN-UHFFFAOYSA-N 2-methyl-1-phenylpropan-2-ol Chemical compound CC(C)(O)CC1=CC=CC=C1 RIWRBSMFKVOJMN-UHFFFAOYSA-N 0.000 description 1
- YUDPTGPSBJVHCN-JZYAIQKZSA-N 4-Methylumbelliferyl-alpha-D-glucopyranoside Chemical compound C1=CC=2C(C)=CC(=O)OC=2C=C1O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O YUDPTGPSBJVHCN-JZYAIQKZSA-N 0.000 description 1
- OCKGFTQIICXDQW-ZEQRLZLVSA-N 5-[(1r)-1-hydroxy-2-[4-[(2r)-2-hydroxy-2-(4-methyl-1-oxo-3h-2-benzofuran-5-yl)ethyl]piperazin-1-yl]ethyl]-4-methyl-3h-2-benzofuran-1-one Chemical compound C1=C2C(=O)OCC2=C(C)C([C@@H](O)CN2CCN(CC2)C[C@H](O)C2=CC=C3C(=O)OCC3=C2C)=C1 OCKGFTQIICXDQW-ZEQRLZLVSA-N 0.000 description 1
- 229920013683 Celanese Polymers 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 101000651439 Homo sapiens Prothrombin Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 229910000861 Mg alloy Inorganic materials 0.000 description 1
- 241001430197 Mollicutes Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000589970 Spirochaetales Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 229910010413 TiO 2 Inorganic materials 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000012468 concentrated sample Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000032798 delamination Effects 0.000 description 1
- 239000002978 dental impression material Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000008393 encapsulating agent Substances 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000003891 environmental analysis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- YLQWCDOCJODRMT-UHFFFAOYSA-N fluoren-9-one Chemical compound C1=CC=C2C(=O)C3=CC=CC=C3C2=C1 YLQWCDOCJODRMT-UHFFFAOYSA-N 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229940039715 human prothrombin Drugs 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000009851 immunogenic response Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 239000011133 lead Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000012299 nitrogen atmosphere Substances 0.000 description 1
- QJAOYSPHSNGHNC-UHFFFAOYSA-N octadecane-1-thiol Chemical compound CCCCCCCCCCCCCCCCCCS QJAOYSPHSNGHNC-UHFFFAOYSA-N 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920006267 polyester film Polymers 0.000 description 1
- 229920006254 polymer film Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000007761 roller coating Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000004447 silicone coating Substances 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000012956 testing procedure Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/01—Drops
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US83839797A | 1997-04-09 | 1997-04-09 | |
| US838397 | 1997-04-09 | ||
| PCT/US1997/014134 WO1998045406A1 (en) | 1997-04-09 | 1997-08-08 | Method and devices for partitioning biological sample liquids into microvolumes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69733366D1 DE69733366D1 (de) | 2005-06-30 |
| DE69733366T2 true DE69733366T2 (de) | 2006-01-26 |
Family
ID=25277013
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69733366T Expired - Lifetime DE69733366T2 (de) | 1997-04-09 | 1997-08-08 | Verfahren und vorrichtungen zur unterteilung biologischer probenflüssigkeiten in mikroflüssigkeitsmengen |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0973863B1 (enExample) |
| JP (1) | JP3981417B2 (enExample) |
| AR (1) | AR012351A1 (enExample) |
| AU (1) | AU735311B2 (enExample) |
| BR (1) | BR9714569B1 (enExample) |
| CA (1) | CA2286195C (enExample) |
| DE (1) | DE69733366T2 (enExample) |
| WO (1) | WO1998045406A1 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102008008256A1 (de) * | 2007-10-08 | 2009-04-09 | M2P-Labs Gmbh | Mikroreaktor |
| DE102011082716A1 (de) * | 2011-09-14 | 2013-03-14 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Indikatorelement mit einer von einer in kontakt zu bringenden substanz abhängigen farbgebung |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6391578B2 (en) | 1997-04-09 | 2002-05-21 | 3M Innovative Properties Company | Method and devices for partitioning biological sample liquids into microvolumes |
| DE19754978C2 (de) * | 1997-12-11 | 2000-07-13 | Bruker Daltonik Gmbh | Probenträger für die MALDI-Massenspektrometrie nebst Verfahren zur Herstellung der Platten und zum Aufbringen der Proben |
| US6893877B2 (en) | 1998-01-12 | 2005-05-17 | Massachusetts Institute Of Technology | Methods for screening substances in a microwell array |
| DE69942697D1 (de) * | 1998-01-12 | 2010-09-30 | Massachusetts Inst Technology | Vorrichtung zur Mikrotestdurchführung |
| US6376619B1 (en) | 1998-04-13 | 2002-04-23 | 3M Innovative Properties Company | High density, miniaturized arrays and methods of manufacturing same |
| US6174699B1 (en) | 1999-03-09 | 2001-01-16 | 3M Innovative Properties Company | Disc assay device with inoculation pad and methods of use |
| WO2000056456A1 (en) | 1999-03-19 | 2000-09-28 | Genencor International, Inc. | Multi-through hole testing plate for high throughput screening |
| DE19926728B4 (de) | 1999-06-11 | 2011-08-18 | 3M Espe Ag, 82229 | Verwendung von Trägermaterialien und diagnostisch nutzbaren Zusatzstoffen in Abbildungsverfahren für intraorale Diagnosezwecke |
| US6482638B1 (en) | 1999-12-09 | 2002-11-19 | 3M Innovative Properties Company | Heat-relaxable substrates and arrays |
| DE10005600A1 (de) * | 2000-02-09 | 2001-08-16 | Bayer Ag | Ultraphobes Flächengebilde mit einer Vielzahl von hydrophilen Bereichen |
| US7332271B2 (en) | 2000-02-18 | 2008-02-19 | Board Of Trustees Of The Leland Stanford Junior University | Apparatus and methods for parallel processing of micro-volume liquid reactions |
| US20020151040A1 (en) | 2000-02-18 | 2002-10-17 | Matthew O' Keefe | Apparatus and methods for parallel processing of microvolume liquid reactions |
| US6492133B1 (en) * | 2000-05-01 | 2002-12-10 | 3M Innovative Properties Company | Reflective disc assay devices, systems and methods |
| US6783735B2 (en) * | 2000-09-15 | 2004-08-31 | Agfa-Gevaert | Web material having wells for combinatorial applications |
| EP1188481A3 (en) * | 2000-09-15 | 2002-05-15 | Agfa-Gevaert | A Microtiter plate having wells for combinatorial applications |
| EP1245278A3 (en) * | 2000-09-15 | 2003-12-10 | Agfa-Gevaert | A web material having microwells for combinatorial applications |
| DE10061195B4 (de) * | 2000-12-08 | 2004-12-02 | 3M Espe Ag | Verwendung von Abformmassen zur Herstellung von Behandlungsvorrichtungen |
| US7115232B2 (en) * | 2001-07-13 | 2006-10-03 | Hudson Gordon S | Fluorescence validation microplate and method of use |
| DE10207616A1 (de) * | 2002-02-22 | 2003-09-04 | Sunyx Surface Nanotechnologies | Ultraphober Probenträger mit funktionalen hydrophilen und/oder oleophilen Bereichen |
| WO2003071274A1 (de) | 2002-02-22 | 2003-08-28 | Sunyx Surface Nanotechnologies Gmbh | Verwendung von ultraphoben oberflächen mit einer vielzahl hydrophiler bereiche zur analyse von proben |
| CA2521999A1 (en) | 2002-12-20 | 2004-09-02 | Biotrove, Inc. | Assay apparatus and method using microfluidic arrays |
| WO2004065009A1 (de) | 2003-01-17 | 2004-08-05 | Greiner Bio-One Gmbh | Probengefäss für analysen |
| DE10321042B4 (de) * | 2003-01-17 | 2006-09-21 | Greiner Bio-One Gmbh | Biochip-Träger |
| US9005549B2 (en) | 2003-01-17 | 2015-04-14 | Greiner Bio-One Gmbh | High throughput polymer-based microarray slide |
| WO2005089945A1 (en) | 2004-03-12 | 2005-09-29 | Biotrove, Inc. | Nanoliter array loading |
| US12070731B2 (en) | 2004-08-04 | 2024-08-27 | Life Technologies Corporation | Methods and systems for aligning dispensing arrays with microfluidic sample arrays |
| US20060105453A1 (en) | 2004-09-09 | 2006-05-18 | Brenan Colin J | Coating process for microfluidic sample arrays |
| JP5081854B2 (ja) * | 2009-03-05 | 2012-11-28 | 三井造船株式会社 | 細胞分離装置、細胞分離システムおよび細胞分離方法 |
| WO2011094865A1 (en) * | 2010-02-02 | 2011-08-11 | Arash Zarrine-Afsar | Fluid sampling device and method of use thereof |
| WO2011097677A1 (en) * | 2010-02-12 | 2011-08-18 | Monash University | Printed multi-zone microzone plates |
| CN103119151B (zh) * | 2010-09-14 | 2015-02-04 | Agc科技玻璃株式会社 | 培养基材 |
| JP5757515B2 (ja) * | 2010-10-15 | 2015-07-29 | 学校法人立命館 | 撥水層を有する液滴保持ツールの製造方法 |
| JP5853475B2 (ja) * | 2011-08-04 | 2016-02-09 | ニプロ株式会社 | 細胞培養シャーレ |
| JP2013047625A (ja) * | 2011-08-29 | 2013-03-07 | Sumitomo Bakelite Co Ltd | マルチウェルプレート |
| DE102012112494A1 (de) * | 2012-12-18 | 2014-07-03 | Karlsruher Institut für Technologie | Verfahren zum Übertragen einer Transferflüssigkeit von einer Vorlagefläche in eine Mehrzahl von diskreten Kompartimenten auf einer Zielfläche und Transferfläche zur Durchführung des Verfahrens |
| JP6156933B2 (ja) * | 2014-01-22 | 2017-07-05 | 国立大学法人 筑波大学 | 細胞培養用デバイス |
| EP3519096A4 (en) * | 2016-09-28 | 2020-06-17 | General Automation Lab Technologies, Inc. | HIGH RESOLUTION SYSTEMS, KITS, APPARATUS AND METHODS FOR DETERMINING BACTERIAL COMMUNITY RELATIONSHIP AND OTHER HIGH THROUGHPUT MICROBIOLOGY APPLICATIONS |
| CN108660068B (zh) * | 2018-02-13 | 2022-04-05 | 臻准生物工程(山西)有限公司 | 生物反应芯片及其制备方法 |
| WO2019236309A1 (en) * | 2018-06-04 | 2019-12-12 | Dupont Nutrition Biosciences Aps | Method for identifying and enumerating specific bacterial populations in an unenriched liquid sample |
| US12162015B2 (en) | 2020-10-13 | 2024-12-10 | Bio-Rad Laboratories, Inc. | System and method for target detection with applications in characterizing food quality and improving food safety |
| WO2022220844A1 (en) * | 2021-04-16 | 2022-10-20 | Plexium, Inc. | Assay devices for combinatorial libraries |
| US20250025137A1 (en) * | 2021-07-09 | 2025-01-23 | Porex Corporation | Device with discrete hydrophilic porous components and method of making the same |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61247965A (ja) * | 1985-04-25 | 1986-11-05 | Susumu Kogyo Kk | 酵素免疫測定法 |
| DE3631066A1 (de) * | 1986-09-12 | 1988-04-14 | Rainer Dylla | Objekttraeger fuer nasse oder feuchte biologische praeparate, z. b. blut |
| JPS6396558A (ja) * | 1986-10-13 | 1988-04-27 | Nitto Electric Ind Co Ltd | 検査プレ−ト用シ−トおよび検査プレ−ト |
| DE3732142C2 (de) * | 1987-09-24 | 1995-12-21 | Heraeus Instr Gmbh | Verfahren zur Herstellung einer mit einer Maske versehenen Glasplatte sowie Stempelkörper zur Durchführung des Verfahrens |
| US5503803A (en) * | 1988-03-28 | 1996-04-02 | Conception Technologies, Inc. | Miniaturized biological assembly |
| US5229163A (en) * | 1989-12-21 | 1993-07-20 | Hoffmann-La Roche Inc. | Process for preparing a microtiter tray for immunometric determinations |
| JPH04265860A (ja) * | 1991-02-21 | 1992-09-22 | Sekisui Chem Co Ltd | 免疫測定検査用凝集判定プレート |
| WO1997012242A1 (en) * | 1995-09-27 | 1997-04-03 | Mercury Diagnostics Inc. | Direct reading chemical test strip for aqueous solutions |
-
1997
- 1997-08-08 EP EP97935341A patent/EP0973863B1/en not_active Expired - Lifetime
- 1997-08-08 JP JP54272898A patent/JP3981417B2/ja not_active Expired - Lifetime
- 1997-08-08 BR BRPI9714569-6A patent/BR9714569B1/pt not_active IP Right Cessation
- 1997-08-08 WO PCT/US1997/014134 patent/WO1998045406A1/en not_active Ceased
- 1997-08-08 DE DE69733366T patent/DE69733366T2/de not_active Expired - Lifetime
- 1997-08-08 AU AU38299/97A patent/AU735311B2/en not_active Expired
- 1997-08-08 CA CA002286195A patent/CA2286195C/en not_active Expired - Fee Related
-
1998
- 1998-04-07 AR ARP980101578 patent/AR012351A1/es unknown
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102008008256A1 (de) * | 2007-10-08 | 2009-04-09 | M2P-Labs Gmbh | Mikroreaktor |
| DE102011082716A1 (de) * | 2011-09-14 | 2013-03-14 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Indikatorelement mit einer von einer in kontakt zu bringenden substanz abhängigen farbgebung |
Also Published As
| Publication number | Publication date |
|---|---|
| AU3829997A (en) | 1998-10-30 |
| JP3981417B2 (ja) | 2007-09-26 |
| BR9714569B1 (pt) | 2009-05-05 |
| BR9714569A (pt) | 2000-02-15 |
| WO1998045406A1 (en) | 1998-10-15 |
| CA2286195C (en) | 2006-02-14 |
| EP0973863B1 (en) | 2005-05-25 |
| AU735311B2 (en) | 2001-07-05 |
| DE69733366D1 (de) | 2005-06-30 |
| EP0973863A1 (en) | 2000-01-26 |
| AR012351A1 (es) | 2000-10-18 |
| CA2286195A1 (en) | 1998-10-15 |
| JP2001522242A (ja) | 2001-11-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69733366T2 (de) | Verfahren und vorrichtungen zur unterteilung biologischer probenflüssigkeiten in mikroflüssigkeitsmengen | |
| US6391578B2 (en) | Method and devices for partitioning biological sample liquids into microvolumes | |
| DE69834493T2 (de) | Verfahren und gerät zum nachweis und zur zählung von mikroorganismen | |
| US6696286B1 (en) | Method and devices for detecting and enumerating microorganisms | |
| DE69619395T2 (de) | Mehrzoniger sterilitätsindikator | |
| DE69718245T2 (de) | Druckempfindliches klebendes Blatt zum Nachweis von Mikroorganismen und Methode zum Nachweis von Mikroorganismen | |
| DE2238251C3 (de) | Kammer für mikrobiologische Arbeiten | |
| WO2003025113A2 (de) | Verfahren zur kultivierung und analyse mikrobieller einzelzellkulturen | |
| DE69329117T2 (de) | Vorrichtung zur kultivierung von mikroorganismen | |
| DE60025504T2 (de) | Reflektierende scheibennachweisvorrichtungen | |
| DE2320946B2 (de) | Vorrichtung für mikrobiologisches Arbeiten | |
| DE69908658T2 (de) | Testgerät und Verfahren zum Nachweis und Aufzählung von Mikroorganismen | |
| DE60121206T2 (de) | Verfahren zum einfangen luftgebundener mikroorganismen mittels wasserlöslicher polymere | |
| AU744534B2 (en) | Disc assay devices and methods of use | |
| DE19751581C2 (de) | Verfahren und Vorrichtung zur Prüfung von Materialien hinsichtlich ihrer potentiellen antimikrobiellen Wirksamkeit und der Adhäsion auf ihrer Oberfläche | |
| DE2826651A1 (de) | Vorrichtung und verfahren fuer die testung von untersuchungsfluessigkeiten | |
| JPH06181745A (ja) | 化学的及び微生物学的試験用用具 | |
| EP0075215B1 (de) | Vorrichtungen zum Nachweis von Mikroorganismen hemmenden Stoffen | |
| AT523906B1 (de) | Inkubationskammer | |
| MXPA99009030A (en) | Method and devices for partitioning biological sample liquids into microvolumes | |
| DE69223045T2 (de) | Vorrichtung zur Durchführung einer Vielzahl von mikrobiologischen Untersuchungen | |
| CH536494A (de) | Verfahren zur Durchführung von labormässigen Diffusionsversuchen und Wirkstofftragkörper zur Ausführung des Verfahrens | |
| DE1932789A1 (de) | Zum einmaligen Gebrauch bestimmte Naehrbodenanordnung fuer Bakterienkulturen | |
| DE10147513A1 (de) | Verfahren zur Minimierung der Verdunstung in Probenträgern | |
| DE2005543B (de) | Objektträger |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |