CA2286195C - Method and devices for partitioning biological sample liquids into microvolumes - Google Patents
Method and devices for partitioning biological sample liquids into microvolumes Download PDFInfo
- Publication number
- CA2286195C CA2286195C CA002286195A CA2286195A CA2286195C CA 2286195 C CA2286195 C CA 2286195C CA 002286195 A CA002286195 A CA 002286195A CA 2286195 A CA2286195 A CA 2286195A CA 2286195 C CA2286195 C CA 2286195C
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- liquid
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- culture device
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- Expired - Fee Related
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/01—Drops
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US83839797A | 1997-04-09 | 1997-04-09 | |
| US08/838397 | 1997-04-09 | ||
| PCT/US1997/014134 WO1998045406A1 (en) | 1997-04-09 | 1997-08-08 | Method and devices for partitioning biological sample liquids into microvolumes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2286195A1 CA2286195A1 (en) | 1998-10-15 |
| CA2286195C true CA2286195C (en) | 2006-02-14 |
Family
ID=25277013
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002286195A Expired - Fee Related CA2286195C (en) | 1997-04-09 | 1997-08-08 | Method and devices for partitioning biological sample liquids into microvolumes |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0973863B1 (enExample) |
| JP (1) | JP3981417B2 (enExample) |
| AR (1) | AR012351A1 (enExample) |
| AU (1) | AU735311B2 (enExample) |
| BR (1) | BR9714569B1 (enExample) |
| CA (1) | CA2286195C (enExample) |
| DE (1) | DE69733366T2 (enExample) |
| WO (1) | WO1998045406A1 (enExample) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6391578B2 (en) | 1997-04-09 | 2002-05-21 | 3M Innovative Properties Company | Method and devices for partitioning biological sample liquids into microvolumes |
| DE19754978C2 (de) * | 1997-12-11 | 2000-07-13 | Bruker Daltonik Gmbh | Probenträger für die MALDI-Massenspektrometrie nebst Verfahren zur Herstellung der Platten und zum Aufbringen der Proben |
| EP1714699B1 (en) * | 1998-01-12 | 2010-08-18 | Massachusetts Institute of Technology | System for analyzing a plurality of samples |
| US6893877B2 (en) | 1998-01-12 | 2005-05-17 | Massachusetts Institute Of Technology | Methods for screening substances in a microwell array |
| US6376619B1 (en) | 1998-04-13 | 2002-04-23 | 3M Innovative Properties Company | High density, miniaturized arrays and methods of manufacturing same |
| US6174699B1 (en) | 1999-03-09 | 2001-01-16 | 3M Innovative Properties Company | Disc assay device with inoculation pad and methods of use |
| BR0009164A (pt) | 1999-03-19 | 2001-12-26 | Genencor Int | Placa de teste de múltiplos furos vazados paraseparação de alto desempenho |
| DE19926728B4 (de) | 1999-06-11 | 2011-08-18 | 3M Espe Ag, 82229 | Verwendung von Trägermaterialien und diagnostisch nutzbaren Zusatzstoffen in Abbildungsverfahren für intraorale Diagnosezwecke |
| US6482638B1 (en) | 1999-12-09 | 2002-11-19 | 3M Innovative Properties Company | Heat-relaxable substrates and arrays |
| DE10005600A1 (de) * | 2000-02-09 | 2001-08-16 | Bayer Ag | Ultraphobes Flächengebilde mit einer Vielzahl von hydrophilen Bereichen |
| US20020151040A1 (en) | 2000-02-18 | 2002-10-17 | Matthew O' Keefe | Apparatus and methods for parallel processing of microvolume liquid reactions |
| WO2001061054A2 (en) | 2000-02-18 | 2001-08-23 | Board Of Trustees Of The Leland Stanford Junior University | Apparatus and methods for parallel processing of micro-volume liquid reactions |
| US6492133B1 (en) * | 2000-05-01 | 2002-12-10 | 3M Innovative Properties Company | Reflective disc assay devices, systems and methods |
| EP1188481A3 (en) * | 2000-09-15 | 2002-05-15 | Agfa-Gevaert | A Microtiter plate having wells for combinatorial applications |
| US6783735B2 (en) * | 2000-09-15 | 2004-08-31 | Agfa-Gevaert | Web material having wells for combinatorial applications |
| EP1245278A3 (en) * | 2000-09-15 | 2003-12-10 | Agfa-Gevaert | A web material having microwells for combinatorial applications |
| DE10061195B4 (de) * | 2000-12-08 | 2004-12-02 | 3M Espe Ag | Verwendung von Abformmassen zur Herstellung von Behandlungsvorrichtungen |
| US7115232B2 (en) * | 2001-07-13 | 2006-10-03 | Hudson Gordon S | Fluorescence validation microplate and method of use |
| DE10207616A1 (de) * | 2002-02-22 | 2003-09-04 | Sunyx Surface Nanotechnologies | Ultraphober Probenträger mit funktionalen hydrophilen und/oder oleophilen Bereichen |
| WO2003071274A1 (de) | 2002-02-22 | 2003-08-28 | Sunyx Surface Nanotechnologies Gmbh | Verwendung von ultraphoben oberflächen mit einer vielzahl hydrophiler bereiche zur analyse von proben |
| CA2521999A1 (en) | 2002-12-20 | 2004-09-02 | Biotrove, Inc. | Assay apparatus and method using microfluidic arrays |
| DE10321042B4 (de) * | 2003-01-17 | 2006-09-21 | Greiner Bio-One Gmbh | Biochip-Träger |
| US9005549B2 (en) | 2003-01-17 | 2015-04-14 | Greiner Bio-One Gmbh | High throughput polymer-based microarray slide |
| US8007744B2 (en) | 2003-01-17 | 2011-08-30 | Greiner Bio-One Gmbh | Sample container for analyses |
| AU2005222618A1 (en) | 2004-03-12 | 2005-09-29 | Biotrove, Inc. | Nanoliter array loading |
| US12070731B2 (en) | 2004-08-04 | 2024-08-27 | Life Technologies Corporation | Methods and systems for aligning dispensing arrays with microfluidic sample arrays |
| US20060105453A1 (en) | 2004-09-09 | 2006-05-18 | Brenan Colin J | Coating process for microfluidic sample arrays |
| DE102008008256A1 (de) * | 2007-10-08 | 2009-04-09 | M2P-Labs Gmbh | Mikroreaktor |
| JP5081854B2 (ja) * | 2009-03-05 | 2012-11-28 | 三井造船株式会社 | 細胞分離装置、細胞分離システムおよび細胞分離方法 |
| US20130053281A1 (en) * | 2010-02-02 | 2013-02-28 | Arash Zarrine-Afsar | Fluid sampling device and method of use thereof |
| WO2011097677A1 (en) * | 2010-02-12 | 2011-08-18 | Monash University | Printed multi-zone microzone plates |
| JP5921437B2 (ja) * | 2010-09-14 | 2016-05-24 | Agcテクノグラス株式会社 | 培養基材 |
| JP5757515B2 (ja) * | 2010-10-15 | 2015-07-29 | 学校法人立命館 | 撥水層を有する液滴保持ツールの製造方法 |
| JP5853475B2 (ja) * | 2011-08-04 | 2016-02-09 | ニプロ株式会社 | 細胞培養シャーレ |
| JP2013047625A (ja) * | 2011-08-29 | 2013-03-07 | Sumitomo Bakelite Co Ltd | マルチウェルプレート |
| DE102011082716A1 (de) * | 2011-09-14 | 2013-03-14 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Indikatorelement mit einer von einer in kontakt zu bringenden substanz abhängigen farbgebung |
| DE102012112494A1 (de) * | 2012-12-18 | 2014-07-03 | Karlsruher Institut für Technologie | Verfahren zum Übertragen einer Transferflüssigkeit von einer Vorlagefläche in eine Mehrzahl von diskreten Kompartimenten auf einer Zielfläche und Transferfläche zur Durchführung des Verfahrens |
| JP6156933B2 (ja) * | 2014-01-22 | 2017-07-05 | 国立大学法人 筑波大学 | 細胞培養用デバイス |
| JP2019531730A (ja) * | 2016-09-28 | 2019-11-07 | ジェネラル オートメーション ラボ テクノロジーズ インコーポレイテッド | 細菌群の関係決定及び他のハイスループット微生物学適用高分解能システム、キット、装置、並びに方法 |
| CN108660068B (zh) * | 2018-02-13 | 2022-04-05 | 臻准生物工程(山西)有限公司 | 生物反应芯片及其制备方法 |
| WO2019236309A1 (en) * | 2018-06-04 | 2019-12-12 | Dupont Nutrition Biosciences Aps | Method for identifying and enumerating specific bacterial populations in an unenriched liquid sample |
| CN116323001A (zh) | 2020-10-13 | 2023-06-23 | 伯乐欧洲有限公司 | 应用于表征食品品质和改进食品安全性的用于靶检测的系统和方法 |
| KR20240013103A (ko) * | 2021-04-16 | 2024-01-30 | 플렉시움 인코포레이티드 | 조합 라이브러리용 분석 디바이스 |
| US20250025137A1 (en) * | 2021-07-09 | 2025-01-23 | Porex Corporation | Device with discrete hydrophilic porous components and method of making the same |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61247965A (ja) * | 1985-04-25 | 1986-11-05 | Susumu Kogyo Kk | 酵素免疫測定法 |
| DE3631066A1 (de) * | 1986-09-12 | 1988-04-14 | Rainer Dylla | Objekttraeger fuer nasse oder feuchte biologische praeparate, z. b. blut |
| JPS6396558A (ja) * | 1986-10-13 | 1988-04-27 | Nitto Electric Ind Co Ltd | 検査プレ−ト用シ−トおよび検査プレ−ト |
| DE3732142C2 (de) * | 1987-09-24 | 1995-12-21 | Heraeus Instr Gmbh | Verfahren zur Herstellung einer mit einer Maske versehenen Glasplatte sowie Stempelkörper zur Durchführung des Verfahrens |
| US5503803A (en) * | 1988-03-28 | 1996-04-02 | Conception Technologies, Inc. | Miniaturized biological assembly |
| US5229163A (en) * | 1989-12-21 | 1993-07-20 | Hoffmann-La Roche Inc. | Process for preparing a microtiter tray for immunometric determinations |
| JPH04265860A (ja) * | 1991-02-21 | 1992-09-22 | Sekisui Chem Co Ltd | 免疫測定検査用凝集判定プレート |
| AU7374796A (en) * | 1995-09-27 | 1997-04-17 | Mercury Diagnostics Inc. | Direct reading chemical test strip for aqueous solutions |
-
1997
- 1997-08-08 WO PCT/US1997/014134 patent/WO1998045406A1/en not_active Ceased
- 1997-08-08 EP EP97935341A patent/EP0973863B1/en not_active Expired - Lifetime
- 1997-08-08 DE DE69733366T patent/DE69733366T2/de not_active Expired - Lifetime
- 1997-08-08 AU AU38299/97A patent/AU735311B2/en not_active Expired
- 1997-08-08 JP JP54272898A patent/JP3981417B2/ja not_active Expired - Lifetime
- 1997-08-08 CA CA002286195A patent/CA2286195C/en not_active Expired - Fee Related
- 1997-08-08 BR BRPI9714569-6A patent/BR9714569B1/pt not_active IP Right Cessation
-
1998
- 1998-04-07 AR ARP980101578 patent/AR012351A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0973863B1 (en) | 2005-05-25 |
| JP3981417B2 (ja) | 2007-09-26 |
| DE69733366T2 (de) | 2006-01-26 |
| BR9714569A (pt) | 2000-02-15 |
| AR012351A1 (es) | 2000-10-18 |
| BR9714569B1 (pt) | 2009-05-05 |
| AU735311B2 (en) | 2001-07-05 |
| WO1998045406A1 (en) | 1998-10-15 |
| EP0973863A1 (en) | 2000-01-26 |
| JP2001522242A (ja) | 2001-11-13 |
| DE69733366D1 (de) | 2005-06-30 |
| AU3829997A (en) | 1998-10-30 |
| CA2286195A1 (en) | 1998-10-15 |
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