DE60021160T2 - Protein-deamiderendes Enzym, dafür kodierendes Gen, enzym-produzierendes Mikroorganismus, Verfahren zur Herstellung und Verwendung davon - Google Patents
Protein-deamiderendes Enzym, dafür kodierendes Gen, enzym-produzierendes Mikroorganismus, Verfahren zur Herstellung und Verwendung davon Download PDFInfo
- Publication number
- DE60021160T2 DE60021160T2 DE2000621160 DE60021160T DE60021160T2 DE 60021160 T2 DE60021160 T2 DE 60021160T2 DE 2000621160 DE2000621160 DE 2000621160 DE 60021160 T DE60021160 T DE 60021160T DE 60021160 T2 DE60021160 T2 DE 60021160T2
- Authority
- DE
- Germany
- Prior art keywords
- protein
- enzyme
- proteins
- polypeptide
- deamidating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000623 proteins and genes Proteins 0.000 title claims description 242
- 108090000790 Enzymes Proteins 0.000 title claims description 233
- 102000004190 Enzymes Human genes 0.000 title claims description 225
- 238000000034 method Methods 0.000 title claims description 82
- 244000005700 microbiome Species 0.000 title claims description 29
- 238000002360 preparation method Methods 0.000 title claims description 11
- 230000008569 process Effects 0.000 title claims description 11
- 102000004169 proteins and genes Human genes 0.000 claims description 193
- 235000018102 proteins Nutrition 0.000 claims description 191
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 84
- 230000000694 effects Effects 0.000 claims description 58
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 54
- 229920001184 polypeptide Polymers 0.000 claims description 36
- 125000003368 amide group Chemical group 0.000 claims description 30
- 235000013305 food Nutrition 0.000 claims description 28
- 230000006240 deamidation Effects 0.000 claims description 26
- 108091033319 polynucleotide Proteins 0.000 claims description 26
- 102000040430 polynucleotide Human genes 0.000 claims description 26
- 239000002157 polynucleotide Substances 0.000 claims description 26
- 238000004519 manufacturing process Methods 0.000 claims description 24
- 108060008539 Transglutaminase Proteins 0.000 claims description 20
- 102000003601 transglutaminase Human genes 0.000 claims description 20
- 238000004132 cross linking Methods 0.000 claims description 16
- 241000056141 Chryseobacterium sp. Species 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 11
- 239000013598 vector Substances 0.000 claims description 11
- 241000611330 Chryseobacterium Species 0.000 claims description 10
- 239000000839 emulsion Substances 0.000 claims description 10
- 239000006260 foam Substances 0.000 claims description 10
- 230000009471 action Effects 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 235000021120 animal protein Nutrition 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 241000589565 Flavobacterium Species 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 235000021118 plant-derived protein Nutrition 0.000 claims description 4
- 241000611354 Empedobacter Species 0.000 claims description 3
- 241001467578 Microbacterium Species 0.000 claims description 3
- 241000186361 Actinobacteria <class> Species 0.000 claims description 2
- 241000343666 Cytophagales Species 0.000 claims description 2
- 241001136275 Sphingobacterium Species 0.000 claims description 2
- 230000004048 modification Effects 0.000 claims description 2
- 238000012986 modification Methods 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims 5
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 241000244332 Flavobacteriaceae Species 0.000 claims 1
- 239000004480 active ingredient Substances 0.000 claims 1
- 238000002715 modification method Methods 0.000 claims 1
- 235000013311 vegetables Nutrition 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 description 215
- 238000006243 chemical reaction Methods 0.000 description 65
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 50
- 239000000243 solution Substances 0.000 description 42
- 150000001413 amino acids Chemical class 0.000 description 35
- 239000002609 medium Substances 0.000 description 33
- 108020004414 DNA Proteins 0.000 description 29
- 239000002773 nucleotide Substances 0.000 description 25
- 125000003729 nucleotide group Chemical group 0.000 description 25
- 239000013615 primer Substances 0.000 description 25
- 239000000758 substrate Substances 0.000 description 24
- 239000004202 carbamide Substances 0.000 description 22
- 108091005804 Peptidases Proteins 0.000 description 19
- 239000012634 fragment Substances 0.000 description 18
- 239000004365 Protease Substances 0.000 description 17
- 108010073771 Soybean Proteins Proteins 0.000 description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 16
- 235000019710 soybean protein Nutrition 0.000 description 16
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 15
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 15
- 235000019419 proteases Nutrition 0.000 description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- 108010068370 Glutens Proteins 0.000 description 14
- 235000021312 gluten Nutrition 0.000 description 14
- 108091006028 deamidated proteins Proteins 0.000 description 13
- 239000000523 sample Substances 0.000 description 13
- 235000021307 Triticum Nutrition 0.000 description 12
- 241000209140 Triticum Species 0.000 description 12
- 229940071162 caseinate Drugs 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 12
- 125000000404 glutamine group Chemical group N[C@@H](CCC(N)=O)C(=O)* 0.000 description 12
- 239000000843 powder Substances 0.000 description 12
- 102000007544 Whey Proteins Human genes 0.000 description 11
- 108010046377 Whey Proteins Proteins 0.000 description 11
- 230000035772 mutation Effects 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 239000006228 supernatant Substances 0.000 description 10
- 235000021119 whey protein Nutrition 0.000 description 10
- SOUXAAOTONMPRY-UHFFFAOYSA-N 2-[[5-amino-5-oxo-2-(phenylmethoxycarbonylamino)pentanoyl]amino]acetic acid Chemical compound OC(=O)CNC(=O)C(CCC(=O)N)NC(=O)OCC1=CC=CC=C1 SOUXAAOTONMPRY-UHFFFAOYSA-N 0.000 description 9
- 102000011632 Caseins Human genes 0.000 description 9
- 108010076119 Caseins Proteins 0.000 description 9
- 108010073324 Glutaminase Proteins 0.000 description 9
- 102000009127 Glutaminase Human genes 0.000 description 9
- 239000005018 casein Substances 0.000 description 9
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 9
- 235000021240 caseins Nutrition 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 9
- 239000007788 liquid Substances 0.000 description 9
- 239000012528 membrane Substances 0.000 description 9
- 108010088751 Albumins Proteins 0.000 description 8
- 102000009027 Albumins Human genes 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 8
- 238000005119 centrifugation Methods 0.000 description 8
- 239000012531 culture fluid Substances 0.000 description 8
- 230000002255 enzymatic effect Effects 0.000 description 8
- 239000013612 plasmid Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 230000009467 reduction Effects 0.000 description 8
- 239000011780 sodium chloride Substances 0.000 description 8
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 239000002253 acid Substances 0.000 description 7
- 230000000692 anti-sense effect Effects 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 7
- 239000013611 chromosomal DNA Substances 0.000 description 7
- 238000009396 hybridization Methods 0.000 description 7
- 230000006872 improvement Effects 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 230000036961 partial effect Effects 0.000 description 7
- 230000002829 reductive effect Effects 0.000 description 7
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 6
- 241000588724 Escherichia coli Species 0.000 description 6
- 235000010469 Glycine max Nutrition 0.000 description 6
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 6
- 238000007792 addition Methods 0.000 description 6
- 125000000539 amino acid group Chemical group 0.000 description 6
- 229960001230 asparagine Drugs 0.000 description 6
- 235000009582 asparagine Nutrition 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 229910052500 inorganic mineral Inorganic materials 0.000 description 6
- 239000011707 mineral Substances 0.000 description 6
- 239000008363 phosphate buffer Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 241000193752 Bacillus circulans Species 0.000 description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 5
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 5
- 244000068988 Glycine max Species 0.000 description 5
- 229940024606 amino acid Drugs 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 239000011575 calcium Substances 0.000 description 5
- 229910052791 calcium Inorganic materials 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 238000010367 cloning Methods 0.000 description 5
- 238000004925 denaturation Methods 0.000 description 5
- 230000036425 denaturation Effects 0.000 description 5
- 229960002989 glutamic acid Drugs 0.000 description 5
- 235000013922 glutamic acid Nutrition 0.000 description 5
- 239000004220 glutamic acid Substances 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 4
- 239000004677 Nylon Substances 0.000 description 4
- 102000035195 Peptidases Human genes 0.000 description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 4
- 244000046052 Phaseolus vulgaris Species 0.000 description 4
- 108090000190 Thrombin Proteins 0.000 description 4
- 230000002378 acidificating effect Effects 0.000 description 4
- 235000019658 bitter taste Nutrition 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 230000002538 fungal effect Effects 0.000 description 4
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 4
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 229920001778 nylon Polymers 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 239000012064 sodium phosphate buffer Substances 0.000 description 4
- 229960004072 thrombin Drugs 0.000 description 4
- 238000000108 ultra-filtration Methods 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- NEAQRZUHTPSBBM-UHFFFAOYSA-N 2-hydroxy-3,3-dimethyl-7-nitro-4h-isoquinolin-1-one Chemical compound C1=C([N+]([O-])=O)C=C2C(=O)N(O)C(C)(C)CC2=C1 NEAQRZUHTPSBBM-UHFFFAOYSA-N 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 3
- 239000004472 Lysine Substances 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 230000002009 allergenic effect Effects 0.000 description 3
- 150000001408 amides Chemical class 0.000 description 3
- 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 230000001804 emulsifying effect Effects 0.000 description 3
- 238000006911 enzymatic reaction Methods 0.000 description 3
- 239000013613 expression plasmid Substances 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 108020001507 fusion proteins Proteins 0.000 description 3
- 102000037865 fusion proteins Human genes 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000008101 lactose Substances 0.000 description 3
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 210000004897 n-terminal region Anatomy 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 230000007065 protein hydrolysis Effects 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000005185 salting out Methods 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 238000006467 substitution reaction Methods 0.000 description 3
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 3
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 2
- ODHCTXKNWHHXJC-VKHMYHEASA-N 5-oxo-L-proline Chemical compound OC(=O)[C@@H]1CCC(=O)N1 ODHCTXKNWHHXJC-VKHMYHEASA-N 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 2
- 239000006171 Britton–Robinson buffer Substances 0.000 description 2
- 108020003215 DNA Probes Proteins 0.000 description 2
- 239000003298 DNA probe Substances 0.000 description 2
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 241000672609 Escherichia coli BL21 Species 0.000 description 2
- 108010024636 Glutathione Proteins 0.000 description 2
- 102000005720 Glutathione transferase Human genes 0.000 description 2
- 108010070675 Glutathione transferase Proteins 0.000 description 2
- 238000012218 Kunkel's method Methods 0.000 description 2
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 2
- 108010033276 Peptide Fragments Proteins 0.000 description 2
- 102000007079 Peptide Fragments Human genes 0.000 description 2
- 108010064851 Plant Proteins Proteins 0.000 description 2
- ODHCTXKNWHHXJC-GSVOUGTGSA-N Pyroglutamic acid Natural products OC(=O)[C@H]1CCC(=O)N1 ODHCTXKNWHHXJC-GSVOUGTGSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 239000012506 Sephacryl® Substances 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 238000010306 acid treatment Methods 0.000 description 2
- ODHCTXKNWHHXJC-UHFFFAOYSA-N acide pyroglutamique Natural products OC(=O)C1CCC(=O)N1 ODHCTXKNWHHXJC-UHFFFAOYSA-N 0.000 description 2
- 125000002252 acyl group Chemical group 0.000 description 2
- 239000013566 allergen Substances 0.000 description 2
- -1 amine compound Chemical class 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000007640 basal medium Substances 0.000 description 2
- 235000015895 biscuits Nutrition 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000012364 cultivation method Methods 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000011188 deamidation reaction Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000004945 emulsification Methods 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 238000005187 foaming Methods 0.000 description 2
- 108010010779 glutamine-pyruvate aminotransferase Proteins 0.000 description 2
- 229960003180 glutathione Drugs 0.000 description 2
- 230000003301 hydrolyzing effect Effects 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 239000003456 ion exchange resin Substances 0.000 description 2
- 229920003303 ion-exchange polymer Polymers 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 239000006072 paste Substances 0.000 description 2
- 125000006239 protecting group Chemical group 0.000 description 2
- 239000011535 reaction buffer Substances 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 235000019515 salmon Nutrition 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 239000001488 sodium phosphate Substances 0.000 description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
- 108700026220 vif Genes Proteins 0.000 description 2
- JIMLDJNLXLMGLX-JTQLQIEISA-N (2s)-5-amino-5-oxo-2-(phenylmethoxycarbonylamino)pentanoic acid Chemical compound NC(=O)CC[C@@H](C(O)=O)NC(=O)OCC1=CC=CC=C1 JIMLDJNLXLMGLX-JTQLQIEISA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- SOUXAAOTONMPRY-NSHDSACASA-N 2-[[(2s)-5-amino-5-oxo-2-(phenylmethoxycarbonylamino)pentanoyl]amino]acetic acid Chemical compound OC(=O)CNC(=O)[C@H](CCC(=O)N)NC(=O)OCC1=CC=CC=C1 SOUXAAOTONMPRY-NSHDSACASA-N 0.000 description 1
- YVGZXTQJQNXIAU-UHFFFAOYSA-N 2-amino-5-(hydroxyamino)-5-oxopentanoic acid Chemical compound OC(=O)C(N)CCC(=O)NO YVGZXTQJQNXIAU-UHFFFAOYSA-N 0.000 description 1
- GHCZTIFQWKKGSB-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;phosphoric acid Chemical compound OP(O)(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O GHCZTIFQWKKGSB-UHFFFAOYSA-N 0.000 description 1
- LJGHYPLBDBRCRZ-UHFFFAOYSA-N 3-(3-aminophenyl)sulfonylaniline Chemical compound NC1=CC=CC(S(=O)(=O)C=2C=C(N)C=CC=2)=C1 LJGHYPLBDBRCRZ-UHFFFAOYSA-N 0.000 description 1
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 1
- 239000004254 Ammonium phosphate Substances 0.000 description 1
- 108010024976 Asparaginase Proteins 0.000 description 1
- 102000015790 Asparaginase Human genes 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 238000009020 BCA Protein Assay Kit Methods 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 description 1
- 241000611351 Bergeyella Species 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102100035882 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 241000589585 Chryseobacterium balustinum Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 230000033616 DNA repair Effects 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 102100023933 Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial Human genes 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 108010016626 Dipeptides Proteins 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 239000004129 EU approved improving agent Substances 0.000 description 1
- 241000589566 Elizabethkingia meningoseptica Species 0.000 description 1
- 241000589586 Empedobacter brevis Species 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 241000230562 Flavobacteriia Species 0.000 description 1
- 208000004262 Food Hypersensitivity Diseases 0.000 description 1
- 206010016946 Food allergy Diseases 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 102000016901 Glutamate dehydrogenase Human genes 0.000 description 1
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 241000235649 Kluyveromyces Species 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- 241000194036 Lactococcus Species 0.000 description 1
- 102000008192 Lactoglobulins Human genes 0.000 description 1
- 108010060630 Lactoglobulins Proteins 0.000 description 1
- IMSOBGJSYSFTKG-PKPIPKONSA-N Lysinoalanine Chemical compound OC(=O)[C@@H](N)CCCCNCC(N)C(O)=O IMSOBGJSYSFTKG-PKPIPKONSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 108010070551 Meat Proteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000014171 Milk Proteins Human genes 0.000 description 1
- 108010011756 Milk Proteins Proteins 0.000 description 1
- 244000294411 Mirabilis expansa Species 0.000 description 1
- 235000015429 Mirabilis expansa Nutrition 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 102000003505 Myosin Human genes 0.000 description 1
- 108060008487 Myosin Proteins 0.000 description 1
- 241001291960 Myroides Species 0.000 description 1
- 241001638144 Myroides odoratus DSM 2801 Species 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 108010058846 Ovalbumin Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 241000605114 Pedobacter heparinus Species 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 108010001441 Phosphopeptides Proteins 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 101900232935 Pyrococcus furiosus DNA polymerase Proteins 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 241000589595 Sphingobacterium spiritivorum Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 description 1
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 229940043376 ammonium acetate Drugs 0.000 description 1
- 239000001099 ammonium carbonate Substances 0.000 description 1
- 235000012501 ammonium carbonate Nutrition 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 229910000148 ammonium phosphate Inorganic materials 0.000 description 1
- 235000019289 ammonium phosphates Nutrition 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 229960003272 asparaginase Drugs 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-M asparaginate Chemical compound [O-]C(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-M 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 235000008452 baby food Nutrition 0.000 description 1
- 235000015241 bacon Nutrition 0.000 description 1
- 230000037429 base substitution Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 101150039352 can gene Proteins 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000003729 cation exchange resin Substances 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- HNEGQIOMVPPMNR-IHWYPQMZSA-N citraconic acid Chemical compound OC(=O)C(/C)=C\C(O)=O HNEGQIOMVPPMNR-IHWYPQMZSA-N 0.000 description 1
- 229940018557 citraconic acid Drugs 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000004581 coalescence Methods 0.000 description 1
- 235000014156 coffee whiteners Nutrition 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 235000014510 cooky Nutrition 0.000 description 1
- 239000002285 corn oil Substances 0.000 description 1
- 235000005687 corn oil Nutrition 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 108010011219 dUTP pyrophosphatase Proteins 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 235000021245 dietary protein Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013332 fish product Nutrition 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 235000020932 food allergy Nutrition 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000000887 hydrating effect Effects 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000003402 intramolecular cyclocondensation reaction Methods 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 159000000014 iron salts Chemical class 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000002960 lipid emulsion Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 150000002696 manganese Chemical class 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 239000008268 mayonnaise Substances 0.000 description 1
- 235000010746 mayonnaise Nutrition 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 235000021239 milk protein Nutrition 0.000 description 1
- 235000013536 miso Nutrition 0.000 description 1
- 108091005573 modified proteins Proteins 0.000 description 1
- 102000035118 modified proteins Human genes 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000001053 orange pigment Substances 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 229940092253 ovalbumin Drugs 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- 235000015927 pasta Nutrition 0.000 description 1
- 235000014594 pastries Nutrition 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 150000003016 phosphoric acids Chemical class 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 159000000001 potassium salts Chemical class 0.000 description 1
- 150000003141 primary amines Chemical class 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 230000009145 protein modification Effects 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000008234 soft water Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 229940001941 soy protein Drugs 0.000 description 1
- 229940071440 soy protein isolate Drugs 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 239000003774 sulfhydryl reagent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 150000003751 zinc Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Nutrition Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP34504499 | 1999-12-03 | ||
| JP34504499 | 1999-12-03 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60021160D1 DE60021160D1 (de) | 2005-08-11 |
| DE60021160T2 true DE60021160T2 (de) | 2006-05-11 |
Family
ID=18373917
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2000621160 Expired - Lifetime DE60021160T2 (de) | 1999-12-03 | 2000-12-04 | Protein-deamiderendes Enzym, dafür kodierendes Gen, enzym-produzierendes Mikroorganismus, Verfahren zur Herstellung und Verwendung davon |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1106696B1 (enExample) |
| JP (2) | JP3696500B2 (enExample) |
| DE (1) | DE60021160T2 (enExample) |
| DK (1) | DK1106696T3 (enExample) |
Families Citing this family (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1304592C (zh) * | 2001-02-27 | 2007-03-14 | 天野酶株式会社 | 乳蛋白的脱酰胺化方法和乳蛋白的变性方法 |
| EP1836907B1 (en) * | 2005-01-13 | 2015-08-05 | Ajinomoto Co., Inc. | Processed meat product or fish paste product and process for producing the same |
| WO2006075772A1 (ja) | 2005-01-13 | 2006-07-20 | Ajinomoto Co., Inc. | 乳製品及びその製造方法 |
| WO2007007487A1 (ja) * | 2005-07-07 | 2007-01-18 | Amano Enzyme Inc. | ビール又はビール様飲料の製造方法 |
| TW200942616A (en) | 2008-03-14 | 2009-10-16 | Ajinomoto Kk | Method of denaturing protein with enzymes |
| JP5385894B2 (ja) * | 2008-03-14 | 2014-01-08 | 味の素株式会社 | 食品素材及びその利用方法 |
| ES2389238T3 (es) | 2008-06-19 | 2012-10-24 | Ajinomoto Co., Inc. | Alimento procesado y procedimiento de producción del mismo |
| WO2010029685A1 (ja) | 2008-09-09 | 2010-03-18 | 天野エンザイム株式会社 | 変異型酵素の設計法、調製法、及び変異型酵素 |
| JP5643099B2 (ja) | 2008-09-29 | 2014-12-17 | 味の素株式会社 | 改質乳の製造方法 |
| WO2011024994A1 (ja) | 2009-08-31 | 2011-03-03 | 味の素株式会社 | 低脂肪又は無脂肪ヨーグルト及びその製造方法 |
| MY159840A (en) | 2010-03-04 | 2017-02-15 | Ajinomoto Kk | Coffee whitener,process for producing same,and process for producing beverage |
| WO2011125826A1 (ja) | 2010-03-31 | 2011-10-13 | 味の素株式会社 | アイスクリーム類及びその製造方法 |
| WO2014061728A1 (ja) | 2012-10-17 | 2014-04-24 | 味の素株式会社 | 化粧料組成物 |
| PT2953482T (pt) | 2013-02-05 | 2019-05-13 | Oatly Ab | Base de aveia líquida |
| PL3130671T3 (pl) * | 2014-03-07 | 2019-10-31 | Ajinomoto Kk | Nowa deamidaza białkowa |
| US20170156375A1 (en) * | 2014-05-23 | 2017-06-08 | Frieslandcampina Nederland B.V. | Method for the preparation of an acid dairy drink and said acid dairy drink |
| KR101648539B1 (ko) * | 2014-10-01 | 2016-08-16 | 이현기 | 식충식물로부터 분리한 신규한 미생물 및 그로부터 생성된 단백질분해효소 |
| US11685914B2 (en) | 2017-09-07 | 2023-06-27 | Amano Enzyme Inc. | Stabilised dry protein deamidase composition |
| AU2020226258B2 (en) | 2019-02-21 | 2025-12-04 | Amano Enzyme Europe Ltd. | Prevention of aggregation in plant milk |
| CN120898896A (zh) | 2019-02-21 | 2025-11-07 | 天野酶制品株式会社 | 坚果奶的凝聚的防止 |
| JP7654553B2 (ja) * | 2019-02-26 | 2025-04-01 | アマノ エンザイム ユーエスエー カンパニー，リミテッド | 安定なタンパク質製剤 |
| CA3150687A1 (en) | 2019-09-12 | 2021-03-18 | Amano Enzyme Inc. | METHOD FOR THE PRODUCTION OF A VEGETABLE PROTEIN CONCENTRATE |
| CN115380116A (zh) | 2020-04-03 | 2022-11-22 | 天野酶制品株式会社 | 蛋白质脱酰胺方法 |
| US20220053786A1 (en) | 2020-08-19 | 2022-02-24 | Ajinomoto Co., Inc. | Methods for producing stirred yogurt |
| WO2022045152A1 (ja) | 2020-08-24 | 2022-03-03 | 天野エンザイム株式会社 | タンパク質脱アミド酵素により処理された植物性ミルク |
| JPWO2022059754A1 (enExample) | 2020-09-18 | 2022-03-24 | ||
| CN116193993A (zh) | 2020-09-18 | 2023-05-30 | 天野酶制品株式会社 | 加工椰子奶的制造方法 |
| CN114521592A (zh) | 2020-10-30 | 2022-05-24 | 天野酶制品株式会社 | 口感提高的植物性蛋白质加工物的制造方法 |
| CN114521591A (zh) | 2020-10-30 | 2022-05-24 | 天野酶制品株式会社 | 香味增强的植物性蛋白质饮食品的加工物的制造方法 |
| CN114521593A (zh) | 2020-11-05 | 2022-05-24 | 天野酶制品株式会社 | 提高分散稳定性和/或溶解性的加工植物性奶的制造方法 |
| WO2022102723A1 (ja) | 2020-11-11 | 2022-05-19 | 天野エンザイム株式会社 | 加工植物性タンパク質含有液状組成物の製造方法 |
| WO2022114059A1 (ja) | 2020-11-27 | 2022-06-02 | 天野エンザイム株式会社 | 卵白タンパク質の熱凝固ゲル強度調整剤 |
| EP4257682A4 (en) | 2020-12-04 | 2024-11-13 | Amano Enzyme Inc. | LIQUID ENZYME PREPARATION |
| US20240049746A1 (en) | 2020-12-24 | 2024-02-15 | Amano Enzyme Inc. | Method for producing rice protein-containing liquid composition |
| EP4271198A1 (en) * | 2021-01-04 | 2023-11-08 | Roquette Freres | Oat protein composition of high solubility |
| WO2022168927A1 (ja) * | 2021-02-03 | 2022-08-11 | 天野エンザイム株式会社 | タンパク質脱アミド酵素 |
| WO2022215688A1 (ja) | 2021-04-05 | 2022-10-13 | アマノ エンザイム ヨーロッパ リミテッド | 加工ヘンプタンパク質含有液状組成物の製造方法 |
| CA3219725A1 (en) | 2021-05-21 | 2022-11-24 | Tomohiro Matsuoka | Method for manufacturing processed oat food, beverage or food material |
| US20250338863A1 (en) | 2021-09-21 | 2025-11-06 | Amano Enzyme Inc. | Production method for protein fermented food or beverage |
| JPWO2023085315A1 (enExample) | 2021-11-09 | 2023-05-19 | ||
| US20250024858A1 (en) | 2021-11-09 | 2025-01-23 | Amano Enzyme Inc. | Production method for textured plant protein-containing food product |
| CN114717151B (zh) * | 2021-11-25 | 2023-05-12 | 宜宾五粮液股份有限公司 | 金黄杆菌属新菌种及其用途 |
| EP4470387A1 (en) | 2022-01-27 | 2024-12-04 | Amano Enzyme Inc. | Property modifier for heat-coagulated gel of egg white protein |
| EP4495244A1 (en) * | 2022-03-16 | 2025-01-22 | Amano Enzyme Inc. | Modified protein glutaminase |
| WO2023214554A1 (ja) | 2022-05-06 | 2023-11-09 | 天野エンザイム株式会社 | 植物性タンパク質含有組成物の増粘剤 |
| WO2023214553A1 (ja) | 2022-05-06 | 2023-11-09 | 天野エンザイム株式会社 | 植物性タンパク質含有液状組成物の食感改善剤 |
| EP4523546A4 (en) | 2022-05-12 | 2025-12-10 | Amano Enzyme Inc | PROCESS FOR MANUFACTURING PROCESSED VEGETABLE PROTEIN COMPOSITION |
| US20250313822A1 (en) | 2022-05-23 | 2025-10-09 | Amano Enzyme Inc. | Liquid enzyme preparation |
| CN120379546A (zh) | 2022-07-07 | 2025-07-25 | 天野酶制品株式会社 | 含有植物性蛋白质的组合物的香气变化方法及呈味增强方法 |
| EP4610353A1 (en) | 2022-10-28 | 2025-09-03 | Amano Enzyme Inc. | Emulsifiability improving agent and foamability improving agent for liquid composition containing vegetable protein and oil/fat |
| EP4643648A1 (en) | 2022-12-28 | 2025-11-05 | Amano Enzyme Inc. | Method for improving liquid retainability of vegetable protein-containing dry composition, method for improving foamability during water suspension, and method for improving emulsifiability during water suspension |
| WO2024150831A1 (ja) | 2023-01-12 | 2024-07-18 | 天野エンザイム株式会社 | 加工オートタンパク質含有液状組成物の製造方法 |
| WO2024172142A1 (ja) | 2023-02-15 | 2024-08-22 | 天野エンザイム株式会社 | 加工植物性タンパク質含有液状組成物の製造方法 |
| WO2024210190A1 (ja) | 2023-04-06 | 2024-10-10 | 天野エンザイム株式会社 | 植物性タンパク質含有液状組成物のミネラル富化剤 |
| WO2025037649A1 (ja) * | 2023-08-17 | 2025-02-20 | 天野エンザイム株式会社 | 植物性飲食品の起泡性向上方法 |
| WO2025173761A1 (en) | 2024-02-16 | 2025-08-21 | Ajinomoto Co., Inc. | Novel dairy product and process for production thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3796633A (en) * | 1971-05-10 | 1974-03-12 | Kikkoman Shoyu Co Ltd | Peptidoglutaminase |
| US5082672A (en) * | 1989-06-21 | 1992-01-21 | The United States Of American As Represented By The Secretary Of Agriculture | Enzymatic deamidation of food proteins for improved food use |
| DE69736247T2 (de) * | 1996-05-20 | 2007-05-24 | Novozymes A/S | Verfahren zur herstellung von proteinhydrolysate |
| JPH1175835A (ja) * | 1997-08-29 | 1999-03-23 | Amano Pharmaceut Co Ltd | 脱アミド化酵素及び当該酵素の測定法 |
| US6251651B1 (en) * | 1998-06-04 | 2001-06-26 | Amano Pharmaceutical Co., Ltd. | Protein-deamidating enzyme, gene encoding the same, production process therefor, and use thereof |
-
2000
- 2000-12-04 EP EP20000310768 patent/EP1106696B1/en not_active Expired - Lifetime
- 2000-12-04 DK DK00310768T patent/DK1106696T3/da active
- 2000-12-04 DE DE2000621160 patent/DE60021160T2/de not_active Expired - Lifetime
- 2000-12-04 JP JP2000368983A patent/JP3696500B2/ja not_active Expired - Lifetime
-
2004
- 2004-11-26 JP JP2004342158A patent/JP3769289B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE60021160D1 (de) | 2005-08-11 |
| JP2001218590A (ja) | 2001-08-14 |
| JP3696500B2 (ja) | 2005-09-21 |
| EP1106696B1 (en) | 2005-07-06 |
| JP2005052158A (ja) | 2005-03-03 |
| EP1106696A1 (en) | 2001-06-13 |
| DK1106696T3 (da) | 2005-08-01 |
| JP3769289B2 (ja) | 2006-04-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE60021160T2 (de) | Protein-deamiderendes Enzym, dafür kodierendes Gen, enzym-produzierendes Mikroorganismus, Verfahren zur Herstellung und Verwendung davon | |
| DE69917716T2 (de) | Protein-deamiderendes Enzym, dafür kodierendes Gen, Verfahren zur Herstellung und Verwendung davon | |
| US7846709B2 (en) | Protein-deamidating enzyme, microorganism producing the same, gene encoding the same, production process therefor, and use thereof | |
| JP3609648B2 (ja) | 新規蛋白質脱アミド酵素、それをコードする遺伝子、その製造法並びにその用途 | |
| DE69629719T2 (de) | Transglutaminasen aus oomyzeten | |
| DE69434051T2 (de) | Alpha-galactosidase | |
| JP3153237B2 (ja) | タンパク質加水分解物 | |
| DE69712912T2 (de) | Verfahren zur herstellung eines proteinhydrolysates | |
| JPH08512201A (ja) | プロテアーゼ活性を有する酵素 | |
| DE69837475T2 (de) | Polypeptide mit aminopeptidaseaktivität und dafür kodierende nukleinsäuren | |
| EP1371734B1 (en) | Method of deamidation of milk protein and method of denaturation of milk protein | |
| DE69734644T2 (de) | Carboxypeptidasen aus aspergillus oryzae und diese kodierende nukleinsäuren | |
| WO2019049927A1 (ja) | 安定化蛋白質脱アミド酵素乾燥組成物 | |
| DE60132989T2 (de) | L-glutamatoxidase | |
| CN116829701A (zh) | 耐热性蛋白质谷氨酰胺酶 | |
| US6228632B1 (en) | Leucine aminopeptidases produced recombinantly from Aspergillus soyae | |
| DE69331742T2 (de) | Neuartige protease | |
| NZ230911A (en) | Lactobacillus helveticus agc1 and its use in ripening cheddar style cheeses | |
| JP2001057842A (ja) | ケーキ用起泡剤及びその利用食品 | |
| DE69827486T2 (de) | Aminopeptidase, welche von bacillus licheniformis abstammt und verfahren zur herstellung von proteinen des natürlichen typs | |
| DE112023001402T5 (de) | Modifizierte proteinglutaminase | |
| DE112023001290T5 (de) | Modifizierte Proteinglutaminase | |
| DE60209467T2 (de) | Neue aminopeptidase und ihr gen | |
| JPS63267298A (ja) | 血球グロビン由来のポリペプチド | |
| EP1309694A2 (de) | Verfahren zur verbesserten herstellung von cyanophycin und dessen folgeprodukte |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |