DE3371792D1 - A process for the in-vitro transformation of plant protoplasts with plasmid dna - Google Patents

A process for the in-vitro transformation of plant protoplasts with plasmid dna

Info

Publication number
DE3371792D1
DE3371792D1 DE8383200207T DE3371792T DE3371792D1 DE 3371792 D1 DE3371792 D1 DE 3371792D1 DE 8383200207 T DE8383200207 T DE 8383200207T DE 3371792 T DE3371792 T DE 3371792T DE 3371792 D1 DE3371792 D1 DE 3371792D1
Authority
DE
Germany
Prior art keywords
plasmid dna
incubation
plant protoplasts
vitro transformation
polyethylene glycol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8383200207T
Other languages
English (en)
Inventor
Robbert Adriaan Schilperoort
Franciscus Andries Krens
George Joseph Wullems
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universiteit Leiden
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Universiteit Leiden
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universiteit Leiden filed Critical Universiteit Leiden
Application granted granted Critical
Publication of DE3371792D1 publication Critical patent/DE3371792D1/de
Expired legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE8383200207T 1982-02-11 1983-02-10 A process for the in-vitro transformation of plant protoplasts with plasmid dna Expired DE3371792D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL8200523A NL8200523A (nl) 1982-02-11 1982-02-11 Werkwijze voor het in vitro transformeren van planteprotoplasten met plasmide-dna.

Publications (1)

Publication Number Publication Date
DE3371792D1 true DE3371792D1 (en) 1987-07-02

Family

ID=19839239

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8383200207T Expired DE3371792D1 (en) 1982-02-11 1983-02-10 A process for the in-vitro transformation of plant protoplasts with plasmid dna

Country Status (6)

Country Link
US (1) US4684611A (de)
EP (1) EP0086537B1 (de)
JP (1) JPH0630589B2 (de)
AT (1) ATE27463T1 (de)
DE (1) DE3371792D1 (de)
NL (1) NL8200523A (de)

Families Citing this family (233)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60500795A (ja) 1983-01-17 1985-05-30 モンサント　カンパニ− 遺伝子的に形質転換された植物
US8334139B1 (en) 1983-01-17 2012-12-18 Monsanto Technology Llc Plasmids for transforming plant cells
US5231019A (en) 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
GB2159173B (en) * 1984-05-11 1988-10-12 Ciba Geigy Ag Transformation of hereditary material of plants
FI864720A (fi) * 1985-11-22 1987-05-23 Ciba Geigy Ag Direkt gentransmission i plasticider och mitokondrier.
ES2039474T3 (es) * 1986-12-05 1993-10-01 Ciba-Geigy Ag Procedimineto mejorado para la transformacion de protoplastos vegetales.
US5350689A (en) * 1987-05-20 1994-09-27 Ciba-Geigy Corporation Zea mays plants and transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells
EP0292435B1 (de) * 1987-05-20 1995-07-26 Ciba-Geigy Ag Zeamays-Pflanzen und transgenetische Zeamays-Pflanzen, die aus Protoplasten oder aus von Protoplasten erhaltenen Zellen regeneriert wurden
US5128460A (en) * 1989-04-04 1992-07-07 Genelabs Incorporated Recombinant trichosanthin and coding sequence
CA2042093C (en) * 1990-05-09 2002-12-24 Gyula Hadlaczky Cell line carrying an excess of mammalian centromeres
US6605712B1 (en) * 1990-12-20 2003-08-12 Arch Development Corporation Gene transcription and ionizing radiation: methods and compositions
US5850025A (en) * 1991-09-19 1998-12-15 Sibia Neurosciences, Inc. Protection of plants against plant pathogens
US5422108A (en) * 1991-09-19 1995-06-06 Smart Plants International Inc. Protection of plants against plant pathogens
US5750868A (en) * 1994-12-08 1998-05-12 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
US5795753A (en) * 1994-12-08 1998-08-18 Pioneer Hi-Bred International Inc. Reversible nuclear genetic system for male sterility in transgenic plants
EP0757102A1 (de) 1995-08-04 1997-02-05 Plant Genetic Systems N.V. Genetische Transformation durch Verwendung von einem PARP-Inhibitor
US6084164A (en) 1996-03-25 2000-07-04 Pioneer Hi-Bred International, Inc. Sunflower seeds with enhanced saturated fatty acid contents
US6025155A (en) * 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US20020160970A1 (en) * 1996-04-10 2002-10-31 Gyula Hadlaczky Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US6077697A (en) 1996-04-10 2000-06-20 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US20030033617A1 (en) * 1996-04-10 2003-02-13 Gyula Hadlaczky Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
WO1999053050A1 (en) 1998-04-08 1999-10-21 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
CA2256121A1 (en) * 1998-06-08 1999-12-08 Hong Wang Cyclin-dependent kinase inhibitors as plant growth regulators
US6815579B1 (en) 1999-07-22 2004-11-09 The University Of British Columbia Plant long chain fatty acid biosynthetic enzyme
CA2284246A1 (en) 1999-10-01 2001-04-01 Agriculture And Agrifood Canada Of Agriculture And Agri-Food Plant fatty acid desaturases and alleles therefor
EP2345721A1 (de) 1999-11-12 2011-07-20 Fibrogen, Inc. Rekombinante Gelatine in Impfstoffen
CA2395897C (en) 1999-12-28 2011-11-15 Bayer Cropscience N.V. Insecticidal proteins from bacillus thuringiensis
GB0002814D0 (en) 2000-02-09 2000-03-29 Univ York Nucleic acids and their uses
AU2001293545A1 (en) 2000-09-15 2002-03-26 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Modulation of meiotic recombination
US7517975B2 (en) 2000-09-26 2009-04-14 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
ES2343106T3 (es) * 2000-09-28 2010-07-23 BIORIGINAL FOOD & SCIENCE CORP. Fad4, fad5, fad5-2 y fad6, miembros de la familia de desaturasas de acidos grasos y usos de los mismos.
US8716022B2 (en) * 2000-11-17 2014-05-06 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food Modulation of meiotic recombination
ES2397549T3 (es) 2001-01-09 2013-03-07 Bayer Cropscience Nv Proteínas insecticidas de Bacillus thuringiensis
ES2409080T3 (es) * 2001-01-22 2013-06-24 Sangamo Biosciences Inc. Proteínas de unión con dedos de zinc modificadas
CA2340998C (en) 2001-03-21 2012-01-03 Saskatchewan Wheat Pool Selective modification of plant fatty acids
US8034791B2 (en) 2001-04-06 2011-10-11 The University Of Chicago Activation of Egr-1 promoter by DNA damaging chemotherapeutics
US20040242523A1 (en) * 2003-03-06 2004-12-02 Ana-Farber Cancer Institue And The Univiersity Of Chicago Chemo-inducible cancer gene therapy
WO2002080849A2 (en) * 2001-04-06 2002-10-17 University Of Chicago Chemotherapeutic induction of egr-1 promoter activity
US7452705B2 (en) 2001-05-22 2008-11-18 The University Of Chicago N4 virion single-stranded DNA dependent RNA polymerase
MXPA03010967A (es) 2001-05-30 2004-10-28 Chromos Molecular Systems Inc Cromosomas artificiales de plantas, usos de los mismos y metodos para preparar cromosomas artificiales de plantas.
WO2003038112A2 (en) 2001-10-26 2003-05-08 Baylor College Of Medicine A composition and method to alter lean body mass and bone properties in a subject
AR037778A1 (es) 2001-12-11 2004-12-01 Advisys Inc Suplementacion mediada por plasmido para el tratamiento de sujetos cronicamente enfermos
US7262054B2 (en) * 2002-01-22 2007-08-28 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
EP1572902B1 (de) 2002-02-01 2014-06-11 Life Technologies Corporation HOCHWIRKSAME siRNAS ZUR REDUZIERUNG DER EXPRESSION VON ZIELGENEN
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
US20040014956A1 (en) 2002-02-01 2004-01-22 Sequitur, Inc. Double-stranded oligonucleotides
DE10212892A1 (de) 2002-03-20 2003-10-09 Basf Plant Science Gmbh Konstrukte und Verfahren zur Regulation der Genexpression
CN100497378C (zh) 2002-03-22 2009-06-10 拜尔生物科学公司 新的苏云金芽孢杆菌杀昆虫蛋白质
CA2501188C (en) 2002-07-15 2012-05-15 Board Of Regents, The University Of Texas System Combinatorial protein library screening by periplasmic expression
US20040268441A1 (en) 2002-07-19 2004-12-30 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
EP1527183B1 (de) 2002-07-26 2008-08-20 BASF Plant Science GmbH Neue selektionsverfahren
EP2269618A1 (de) 2002-08-12 2011-01-05 Jennerex Biotherapeutics ULC Ein onkolytisches Vaccinia-Virus zur Verwendung in Kombination mit Chemotherapie zur Behandlung von Krebs
TW200424214A (en) * 2003-04-21 2004-11-16 Advisys Inc Plasmid mediated GHRH supplementation for renal failures
US20070224615A1 (en) * 2003-07-09 2007-09-27 Invitrogen Corporation Methods for assaying protein-protein interactions
WO2005007822A2 (en) 2003-07-09 2005-01-27 Sentigen Biosciences, Inc. Method for assaying protein-protein interaction
EP1502954A1 (de) * 2003-07-31 2005-02-02 greenovation Biotech GmbH Gebrauch von Konstrukten mit rekombinanten Sequenzmotifen zur Erhöhung der Genexpression in Moosen
IL157538A0 (en) 2003-08-21 2004-03-28 Bar Ilan Res & Dev Company Ltd Plant resistant to cytoplasm-feeding parasites
WO2005103254A1 (en) * 2004-04-22 2005-11-03 Yissum Research Development Company Of The Hebrew University Of Jerusalem UNIVERSAL TARGET SEQUENCES FOR siRNA GENE SILENCING
DK2302055T3 (da) 2004-11-12 2014-10-13 Asuragen Inc Fremgangsmåder og sammensætninger involverende miRNA og miRNA-inhibitormolekyler
AU2006237317B8 (en) 2005-04-19 2011-05-12 Basf Plant Science Gmbh Improved methods controlling gene expression
CN101300268A (zh) 2005-07-08 2008-11-05 墨西哥国立自治大学 新的具有杀虫活性的细菌蛋白质
KR20090004839A (ko) * 2005-09-07 2009-01-12 제네렉스, 인코포레이티드 Ｇｍ-ｃｓｆ를 발현하는 폭스바이러스를 사용한 전이성및/또는 전신 파종성 암의 전신 치료법
US8980246B2 (en) 2005-09-07 2015-03-17 Sillajen Biotherapeutics, Inc. Oncolytic vaccinia virus cancer therapy
CN101268194A (zh) 2005-09-20 2008-09-17 巴斯福植物科学有限公司 使用ta-siRNA调控基因表达的方法
EP2368569A3 (de) 2006-01-18 2012-05-02 University Of Chicago Zusammensetzungen und Verfahren im Zusammenhang mit Proteinen des Staphylococcus-Bakteriums
AU2007228981B2 (en) 2006-03-21 2012-10-04 BASF Agricultural Solutions Seed US LLC Novel genes encoding insecticidal proteins
US7666677B2 (en) 2006-07-05 2010-02-23 Luis Fabricio Medina-Bolivar Production of stilbenes in plant hairy root cultures
EP2839837B1 (de) 2006-09-15 2019-05-08 Children's Hospital of Eastern Ontario Research Institute Inc. Onkolytisches farmington rhabdovirus
CA2671264C (en) 2006-11-30 2015-11-24 Research Development Foundation Improved immunoglobulin libraries
CN101605903B (zh) 2007-02-06 2012-09-05 巴斯福植物科学有限公司 丙氨酸消旋酶基因对植物赋予线虫抗性的用途
US20100011463A1 (en) 2007-02-06 2010-01-14 Basf Plant Science Gmbh Compositions and Methods Using RNA Interference for Control of Nematodes
CN101605894A (zh) 2007-02-08 2009-12-16 巴斯福植物科学有限公司 用opr3-样基因的rna干扰控制线虫的组合物和方法
MX2009007766A (es) 2007-02-09 2009-07-30 Basf Plant Science Gmbh Composiciones y metodos que utilizan arn de interferencia de tipo cdpk para el control de nematodos.
KR20080084528A (ko) 2007-03-15 2008-09-19 제네렉스 바이오테라퓨틱스 인크. 종양살상형 백시니아 바이러스 암 치료
MX2009008601A (es) 2007-03-15 2009-08-21 Basf Plant Science Gmbh Uso de genes de quitinasa de nematodos con el fin de controlar nematodos que son parasitos de plantas.
CA2681661C (en) * 2007-03-23 2015-11-24 New York University Methods of increasing nitrogen-assimilation capacity in transgenic plants expressing cca1 and glk1
GB0709835D0 (en) 2007-05-22 2007-07-04 Plant Bioscience Ltd Composition and method for modulating plant root hair development
US9994621B2 (en) * 2007-06-01 2018-06-12 Bayer Cropscience N.V. Genes encoding insecticidal proteins
CN101802201A (zh) 2007-08-03 2010-08-11 先锋高级育种国际公司 影响植物雄性生育力的msca1核苷酸序列以及使用其的方法
CN101951948B (zh) 2007-08-31 2015-12-09 芝加哥大学 与针对葡萄球菌性肺部疾病和病症进行免疫相关的方法和组合物
EP2554674B1 (de) 2007-12-21 2014-11-05 Keygene N.V. Trichomenspezifische Promotoren
EP2944649A1 (de) 2008-01-10 2015-11-18 Research Development Foundation Impfstoffe und diagnostika für ehrlichiosen
WO2009132850A1 (en) * 2008-05-01 2009-11-05 Bayer Bioscience N.V. Armyworm insect resistance management in transgenic plants
CN101280315B (zh) 2008-05-20 2010-09-01 中国农业科学院油料作物研究所 毛白杨木质素单体合成基因4-cl及应用
KR101871192B1 (ko) 2008-06-04 2018-06-27 후지필름 셀룰러 다이내믹스, 인코포레이티드 비-바이러스 접근법을 사용한 ｉＰＳ 세포의 생산 방법
CN107988261A (zh) 2008-08-12 2018-05-04 细胞动力国际有限公司 产生ips细胞的方法
EP2328603A4 (de) 2008-08-18 2013-01-02 Univ Maryland Apf-derivate und verwendungsverfahren
MX2011001356A (es) 2008-08-27 2011-03-29 Basf Plant Science Gmbh Plantas transgenicas resistentes a nematodos.
GB2465748B (en) 2008-11-25 2012-04-25 Algentech Sas Plant cell transformation method
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
US9084781B2 (en) 2008-12-10 2015-07-21 Novartis Ag MEK mutations conferring resistance to MEK inhibitors
MX2011005773A (es) 2008-12-11 2011-06-30 Basf Plant Science Gmbh Planta con resistencia a nematodos especificos de raiz.
WO2010084488A1 (en) 2009-01-20 2010-07-29 Ramot At Tel-Aviv University Ltd. Mir-21 promoter driven targeted cancer therapy
GB2467167B (en) 2009-01-26 2013-09-11 Algentech Sas Gene targeting in plants
CN102361987A (zh) 2009-03-20 2012-02-22 巴斯夫植物科学有限公司 抗线虫的转基因植物
EP3281947B1 (de) 2009-04-03 2020-02-12 The University of Chicago Zusammensetzungen und verfahren in verbindung mit protein-a (spa)-varianten
EP2424885B1 (de) 2009-04-28 2016-03-23 Vanderbilt University Zusammensetzungen und verfahren zur behandlung von erkrankungen im zusammenhang mit epithelzellapoptose
KR101811235B1 (ko) 2009-06-05 2017-12-21 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 Ｔ 세포 및 조혈 세포의 재프로그래밍
CA2764528A1 (en) 2009-06-08 2010-12-16 Nunhems B.V. Drought tolerant plants
EP2443241A1 (de) 2009-06-15 2012-04-25 Plant Bioscience Limited Herstellung von viralen kapsiden
CA2766975A1 (en) 2009-07-01 2011-01-06 Bayer Bioscience N.V. Methods and means for obtaining plants with enhanced glyphosate tolerance
ES2550202T3 (es) * 2009-08-03 2015-11-05 Recombinetics, Inc. Métodos y composiciones para la modificación dirigida de genes
BR112012002454A2 (pt) 2009-08-05 2017-07-04 Chromocell Corp plantas, micróbios e organismos melhorados
CA2770572A1 (en) 2009-08-25 2011-03-03 Basf Plant Science Company Gmbh Nematode-resistant transgenic plants
BR112012005774B8 (pt) 2009-09-14 2023-02-14 Jennerex Inc uso de um inibidor de quinase antiangiogênico no tratamento de câncer
EP2357239A1 (de) 2009-10-29 2011-08-17 Universität zu Köln Verfahren und Mittel für ein auswählbares Markierungssystem bei Pflanzen
EP2510090A1 (de) 2009-12-09 2012-10-17 BASF Plant Science Company GmbH Verfahren zur steigerung der resistenz von pflanzen gegenüber pilzen durch ausschaltung des smt1-pilzgens
MX337062B (es) 2009-12-10 2016-02-11 Ottawa Hospital Res Inst Rabdovirus oncolítico.
US10080799B2 (en) 2010-02-12 2018-09-25 Arizona Board Of Regents On Behalf Of Arizona State University Methods and compositions related to glycoprotein-immunoglobulin fusions
EP2539362A1 (de) 2010-02-23 2013-01-02 BASF Plant Science Company GmbH Nematodenresistente transgene pflanzen
EP3028699B1 (de) 2010-02-25 2018-03-21 Dana-Farber Cancer Institute, Inc. Braf-mutationen für resistenz gegen braf-inhibitoren
EP2542678B1 (de) 2010-03-04 2017-04-12 InteRNA Technologies B.V. Mirna-molekül, das durch seine quelle definiert ist, und seine therapeutischen verwendungen bei emt assoziierten krebs
US8808699B2 (en) 2010-04-05 2014-08-19 The University Of Chicago Compositions and methods related to protein A (SpA) antibodies as an enhancer of immune response
WO2011126976A1 (en) 2010-04-07 2011-10-13 Vanderbilt University Reovirus vaccines and methods of use therefor
DK2560672T3 (en) 2010-04-19 2014-03-17 Res Dev Foundation RTEF-1 variants and their uses
GB201008720D0 (en) 2010-05-25 2010-07-07 Univ Aberdeen RxLR-leader peptides and protein translocation
CN103220905A (zh) 2010-05-28 2013-07-24 纽海姆有限公司 果实大小增大的植物
EP3889254B1 (de) 2010-06-09 2024-08-14 Dana-Farber Cancer Institute, Inc. Mek1-mutation für resistenz gegenüber raf- und mek-inhibitoren
KR101861168B1 (ko) 2010-06-15 2018-05-31 후지필름 셀룰러 다이내믹스, 인코포레이티드 작은 부피의 말초 혈액으로부터의 유도된 만능 줄기 세포의 생성
SG186290A1 (en) 2010-07-02 2013-01-30 Univ Chicago Compositions and methods related to protein a (spa) variants
NZ704322A (en) 2010-07-06 2016-07-29 Interna Technologies Bv Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway
JP2013531502A (ja) 2010-07-08 2013-08-08 バイエル・クロップサイエンス・エヌ・ヴェー グルコシノレート輸送タンパク質及びその使用
WO2012018933A2 (en) 2010-08-04 2012-02-09 Cellular Dynamics International, Inc. Reprogramming immortalized b cells
EP2614074A1 (de) 2010-09-09 2013-07-17 The University of Chicago Verfahren und zusammensetzungen mit schützenden staphylokokken-antigenen
CN105457016B (zh) 2010-09-22 2022-06-24 科罗拉多大学董事会 Smad7的治疗应用
WO2012074868A2 (en) 2010-12-03 2012-06-07 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
AR087935A1 (es) 2010-12-20 2014-04-30 Basf Plant Science Co Gmbh Plantas transgenicas resistentes a los nematodos
CN103429258B (zh) 2011-01-04 2016-03-09 新罗杰公司 通过施用溶瘤痘苗病毒生成针对肿瘤抗原的抗体和生成肿瘤特异性补体依赖性细胞毒性
EP2474617A1 (de) 2011-01-11 2012-07-11 InteRNA Technologies BV MIR zur Behandlung von Neoangiogenese
US8648230B2 (en) 2011-03-18 2014-02-11 Ms Technologies, Llc Regulatory regions preferentially expressing in non-pollen plant tissue
EP2694534B1 (de) 2011-04-08 2018-06-20 Evaxion Biotech ApS Proteine und nukleinsäuren zur verwendung für impstoffe gegen staphylococcus aureus
US8945588B2 (en) 2011-05-06 2015-02-03 The University Of Chicago Methods and compositions involving protective staphylococcal antigens, such as EBH polypeptides
EP2535416A1 (de) 2011-05-24 2012-12-19 BASF Plant Science Company GmbH Entwicklung einer Phytophthora-resistenten Kartoffel mit verbessertem Ertrag
EP2714901B1 (de) 2011-05-31 2024-09-18 Keygene N.V. Schädlingsresistente pflanzen
CA2872045A1 (en) 2011-06-08 2012-12-13 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
EP2744517B1 (de) 2011-08-15 2019-03-13 The University of Chicago Zusammensetzungen und verfahren in zusammenhang mit antikörpern gegen das staphylokokken-protein a
US20130101664A1 (en) 2011-08-18 2013-04-25 Donald W. Kufe Muc1 ligand traps for use in treating cancers
WO2013053899A1 (en) 2011-10-12 2013-04-18 Moeller Niels Iversen Peptides derived from campylobacter jejuni and their use in vaccination
IN2014MN00853A (de) 2011-10-19 2015-04-17 Keygene Nv
ES2886147T3 (es) 2011-12-22 2021-12-16 Interna Tech B V MiARN para el tratamiento del cáncer de cabeza y de cuello
US9644213B2 (en) 2012-03-09 2017-05-09 Board Of Trustees Of Michigan State University Method of enhancing plant drought tolerance by expression of NDR1
US9968668B2 (en) 2012-04-26 2018-05-15 The University Of Chicago Staphylococcal coagulase antigens and methods of their use
CN104703622B (zh) 2012-04-26 2017-05-24 芝加哥大学 与金黄色葡萄球菌疾病期间抵消凝固酶活性的抗体相关的组合物和方法
US20150101077A1 (en) 2012-05-18 2015-04-09 Pioneer Hi-Bred International Inc Inducible promoter sequences for regulated expression and methods of use
US10201556B2 (en) 2012-11-06 2019-02-12 Interna Technologies B.V. Combination for use in treating diseases or conditions associated with melanoma, or treating diseases or conditions associated with activated B-raf pathway
US9598707B2 (en) 2012-11-26 2017-03-21 Arkansas State University-Jonesboro Method to increase the yield of products in plant material
WO2014116721A1 (en) 2013-01-22 2014-07-31 The Arizona Board Of Regents For And On Behalf Of Arizona State University Geminiviral vector for expression of rituximab
US10231397B2 (en) 2013-01-29 2019-03-19 Basf Plant Science Company Gmbh Fungal resistant plants expressing EIN2
CA2897482A1 (en) 2013-01-29 2014-08-07 Basf Plant Science Company Gmbh Fungal resistant plants expressing hcp7
WO2014117990A1 (en) 2013-01-29 2014-08-07 Basf Plant Science Company Gmbh Fungal resistant plants expressing hcp6
CA2901501C (en) 2013-02-21 2023-03-07 Children's Hospital Of Eastern Ontario Research Institute Inc. Vaccine composition
US9783817B2 (en) 2013-03-04 2017-10-10 Arkansas State University Methods of expressing and detecting activity of expansin in plant cells
AR096014A1 (es) 2013-03-08 2015-12-02 Basf Plant Science Co Gmbh PLANTAS RESISTENTES A HONGOS QUE EXPRESAN EL FACTOR DE TRANSCRIPCIÓN Myb (MybTF)
US10456448B2 (en) 2013-03-08 2019-10-29 The Regents Of The University Of Colorado, A Body Corporate PTD-SMAD7 therapeutics
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
DK3033354T3 (en) 2013-08-14 2019-01-14 Inst Of Genetics And Developmental Biology Methods for modulating seed and organ size in plants
JP2016531147A (ja) 2013-09-09 2016-10-06 フィジーン、エルエルシーＦｉｇｅｎｅ， Ｌｌｃ コンドロサイトまたは軟骨型細胞の再生のための遺伝子治療
WO2015070050A1 (en) 2013-11-08 2015-05-14 Baylor Research Institute Nuclear loclization of glp-1 stimulates myocardial regeneration and reverses heart failure
US20170002064A1 (en) 2013-11-08 2017-01-05 The Board Of Regents Of The University Of Texas System Vh4 antibodies against gray matter neuron and astrocyte
GB201319876D0 (en) 2013-11-11 2013-12-25 Plant Bioscience Ltd Methods of modulating seed and organ size in plants
EP4227685A3 (de) 2013-12-03 2024-02-28 Evaxion Biotech A/S Proteine und nukleinsäuren für impfstoffe gegen staphylococcus aureus
US9714429B2 (en) 2014-01-28 2017-07-25 Arkansas State University Regulatory sequence of cupin family gene
EP3099719B1 (de) 2014-01-29 2020-04-01 Dana-Farber Cancer Institute, Inc. Antikörper gegen die muc1-c/extrazelluläre domäne (/muc1-c/ecd)
US10214741B2 (en) 2014-02-14 2019-02-26 University Of Utah Research Foundation Methods and compositions for inhibiting retinopathy of prematurity
EP3110836A1 (de) 2014-02-25 2017-01-04 Research Development Foundation Sty-peptide zur hemmung von angiogenese
EP3218002B1 (de) 2014-11-13 2020-08-19 Evaxion Biotech ApS Aus acinetobacter baumannii abgeleitete peptide und deren verwendung bei der impfung
EP3244918A2 (de) 2015-01-12 2017-11-22 Evaxion Biotech ApS Proteine und nukleinsäuren zur verwendung für impfstoffe gegen klebsiella pneumoniae
US20180002656A1 (en) 2015-01-28 2018-01-04 Sabic Global Technologies B.V. Methods and compositions for high-efficiency production of biofuel and/or biomass
CN107429259A (zh) 2015-02-04 2017-12-01 巴斯夫植物科学有限公司 通过增加莨菪亭含量，增加转基因植物中大豆锈病抗性的方法
EP3259346B1 (de) 2015-02-20 2024-08-07 Baylor College of Medicine P63-inaktivierung zur behandlung von herzinsuffizienz
WO2017005670A1 (en) 2015-07-04 2017-01-12 Evaxion Biotech Aps Proteins and nucleic acids useful in vaccines targeting pseudomonas aeruginosa
EP3344774A1 (de) 2015-09-04 2018-07-11 Keygene N.V. Diplospory-gen
WO2017053433A1 (en) 2015-09-21 2017-03-30 Modern Meadow, Inc. Fiber reinforced tissue composites
TW202344686A (zh) 2015-10-30 2023-11-16 美國加利福尼亞大學董事會 從幹細胞產生t細胞之方法及使用該t細胞之免疫療法
FI3373968T3 (fi) 2015-11-09 2024-07-17 Childrens Hospital Philadelphia Glypikaani 2 syövän merkkiaineena ja terapeuttisena kohteena
US11286354B2 (en) 2016-02-15 2022-03-29 Modern Meadow, Inc. Method for making a biofabricated material containing collagen fibrils
EP3419654B1 (de) 2016-02-22 2022-04-27 Evaxion Biotech A/S In impfstoffen gegen staphylococcus aureus nützliche proteine und nukleinsäuren
AU2017232723B2 (en) 2016-03-15 2023-04-20 National Research Council Of Canada Modulating plant abiotic stress responses using the kanghan gene family
US20190177391A1 (en) 2016-03-31 2019-06-13 Baylor Research Institute Angiopoietin-like protein 8 (angptl8)
WO2017184500A1 (en) 2016-04-18 2017-10-26 Bloomsburg University of Pennsylvania Compositions and methods of delivering molecules to plants
EP3054014A3 (de) 2016-05-10 2016-11-23 BASF Plant Science Company GmbH Benutzung einer fungizids auf transgenen pflanzen
KR102677451B1 (ko) 2016-05-25 2024-06-24 인쎄름 (엥스띠뛰 나씨오날 드 라 쌍떼 에 드 라 흐쉐르슈 메디깔) 암 치료 방법 및 조성물
WO2017216384A1 (en) 2016-06-17 2017-12-21 Evaxion Biotech Aps Vaccination targeting ichthyophthirius multifiliis
EP3260542A1 (de) 2016-06-20 2017-12-27 Algentech Proteinproduktion in pflanzenzellen
AU2017281605B2 (en) 2016-06-20 2021-04-15 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College A green alga bicarbonate transporter and uses thereof
WO2017220787A1 (en) 2016-06-24 2017-12-28 Evaxion Biotech Aps Vaccines against aearomonas salmonicida infection
US11414464B2 (en) 2016-07-22 2022-08-16 Evaxion Biotech A/S Chimeric proteins for inducing immunity towards infection with S. aureus
CN109963590B (zh) 2016-09-02 2024-03-15 加利福尼亚大学董事会 涉及白介素-6受体α结合单链可变片段的方法和组合物
WO2018127545A1 (en) 2017-01-05 2018-07-12 Evaxion Biotech Aps Vaccines targeting pseudomonas aeruginosa
CA3008850A1 (en) 2017-06-29 2018-12-29 Modern Meadow, Inc. Yeast strains and methods for producing collagen
EP3704250A1 (de) 2017-11-03 2020-09-09 InteRNA Technologies B.V. Mirna-molekül, äquivalent, antagomir oder quelle davon zur behandlung und/oder diagnose eines zustands und/oder einer erkrankung im zusammenhang mit neuronaler defizienz oder neuronaler (re)generation
AU2018253595A1 (en) 2017-11-13 2019-05-30 Modern Meadow, Inc. Biofabricated leather articles having zonal properties
WO2019099493A1 (en) 2017-11-14 2019-05-23 Henry Ford Health System Compositions for use in the treatment and prevention of cardiovascular disorders resulting from cerebrovascular injury
KR20200111168A (ko) 2017-11-24 2020-09-28 인쎄름 (엥스띠뛰 나씨오날 드 라 쌍떼 에 드 라 흐쉐르슈 메디깔) 암 치료를 위한 방법 및 조성물
BR112020013715A2 (pt) 2018-01-05 2020-12-01 Ottawa Hospital Research Institute vetores de vaccinia modificados
WO2019145399A1 (en) 2018-01-24 2019-08-01 Evaxion Biotech Aps Vaccines for prophylaxis of s. aureus infections
WO2019185129A1 (en) 2018-03-27 2019-10-03 The University Court Of The University Of Glasgow Bacterial pathogen derived resistance in plants
EP3837372A1 (de) 2018-08-13 2021-06-23 Aarhus Universitet Genetisch veränderte pflanzen, die heterologe rezeptoren exprimieren, die lipo-chitooligosaccharide erkennen
CA3109236A1 (en) 2018-08-13 2020-02-20 Aarhus Universitet Genetically altered lysm receptors with altered agonist specificity and affinity
US20220000932A1 (en) 2018-09-28 2022-01-06 Henry Ford Health System Use of extracellular vesicles in combination with tissue plasminogen activator and/or thrombectomy to treat stroke
WO2020083904A1 (en) 2018-10-22 2020-04-30 Evaxion Biotech Aps Vaccines targeting m. catharrhalis
EP3891287A1 (de) 2018-12-06 2021-10-13 Wageningen Universiteit Verfahren zur genetischen veränderung eines pflanzlichen nin-gens zur reaktion auf cytokinin
CN113286864A (zh) 2019-01-17 2021-08-20 现代牧场股份有限公司 层状胶原材料及其制备方法
US20220143168A1 (en) 2019-02-27 2022-05-12 Evaxion Biotech A/S Vaccines targeting H. influenzae
BR112021018680A2 (pt) 2019-03-21 2021-11-23 Univ Of Essex Enterprises Limited Métodos para aumentar a biomassa em uma planta através da estimulação da regeneração de rubp e transporte de elétrons
WO2020239984A1 (en) 2019-05-29 2020-12-03 Keygene N.V. Gene for parthenogenesis
AR122277A1 (es) 2019-07-11 2022-08-31 Univ California Métodos para la regeneración mejorada de plantas transgénicas utilizando el factor de regulación del crecimiento (grf), el factor de interacción con grf (gif) o genes grf-gif y proteínas quiméricos
AU2020317009A1 (en) 2019-07-19 2022-02-03 The Board Of Trustees Of The Leland Stanford Junior University Chimeric antigen receptors containing Glypican 2 binding domains
GB201911068D0 (en) 2019-08-02 2019-09-18 Univ Edinburgh Modified higher plants
BR112022001945A2 (pt) 2019-08-02 2022-05-03 Hughes Howard Med Inst Motivos proteicos de ligação à rubisco e usos dos mesmos
AU2020333198A1 (en) 2019-08-19 2022-03-17 Aarhus Universitet Modified exopolysaccharide receptors for recognizing and structuring microbiota
CN115175680A (zh) 2019-10-18 2022-10-11 加利福尼亚大学董事会 Plxdc激活剂及其用于治疗血管病症的用途
WO2021140123A1 (en) 2020-01-06 2021-07-15 Evaxion Biotech Aps Vaccines targeting neisseria gonorrhoeae
AR121460A1 (es) 2020-02-28 2022-06-08 Cambridge Entpr Ltd Métodos, plantas y composiciones para superar la supresión de nutrientes de microrrizas
US20230211023A1 (en) 2020-03-25 2023-07-06 Erasmus University Medical Center Rotterdam Reporter system for radionuclide imaging
CN116323952A (zh) 2020-05-19 2023-06-23 奥尔胡斯大学 Lysm受体基序
WO2022053130A1 (en) 2020-09-09 2022-03-17 Sid Alex Group, S.R.O. Antago-mir-155 for treatment of v-src, c-src-tyrosine kinase-induced cancers
IL301700A (en) 2020-10-13 2023-05-01 Keygene Nv Adapted promoter of parthenogenesis gene
WO2022173767A1 (en) 2021-02-09 2022-08-18 University Of Houston System Oncolytic virus for systemic delivery and enhanced anti-tumor activities
WO2022175815A1 (en) 2021-02-19 2022-08-25 Pfizer Inc. Methods of protecting rna
EP4347845A2 (de) 2021-05-26 2024-04-10 The Board of Trustees of the University of Illinois C4-pflanzen mit erhöhter photosynthetischer effizienz
IL309032A (en) 2021-06-03 2024-02-01 Mazen Animal Health Inc Oral administration of coronavirus spike protein to alter cytokine levels and provide passive immunity to newborn pigs
WO2023280807A1 (en) 2021-07-05 2023-01-12 Evaxion Biotech A/S Vaccines targeting neisseria gonorrhoeae
WO2023089556A1 (en) 2021-11-22 2023-05-25 Pfizer Inc. Reducing risk of antigen mimicry in immunogenic medicaments
GB2614309A (en) 2021-12-24 2023-07-05 Stratosvir Ltd Improved vaccinia virus vectors
WO2023144779A1 (en) 2022-01-28 2023-08-03 Pfizer Inc. Coronavirus antigen variants
WO2023178191A1 (en) 2022-03-16 2023-09-21 University Of Houston System Persistent hsv gene delivery system
US20230323480A1 (en) 2022-04-11 2023-10-12 The Regents Of The University Of California Methods of screening for plant gain of function mutations and compositions therefor
WO2023213393A1 (en) 2022-05-04 2023-11-09 Evaxion Biotech A/S Staphylococcal protein variants and truncates
US20240200085A1 (en) 2022-12-15 2024-06-20 Aarhus Universitet Synthetic activation of multimeric transmembrane receptors
WO2024130212A1 (en) 2022-12-16 2024-06-20 Turnstone Biologics Corp. Recombinant vaccinia virus encoding one or more natural killer cell and t lymphocyte inhibitors
US20240271152A1 (en) 2023-02-03 2024-08-15 Aarhus Universitet Enhancing nitrogen fixation with fun
WO2024186630A1 (en) 2023-03-03 2024-09-12 Henry Ford Health System Use of extracellular vesicles for the treatment of cancer

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4394448A (en) * 1978-02-24 1983-07-19 Szoka Jr Francis C Method of inserting DNA into living cells

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