AU581819B2 - Process for the immortalization of human and animal cells - Google Patents

Process for the immortalization of human and animal cells

Info

Publication number
AU581819B2
AU581819B2 AU76771/87A AU7677187A AU581819B2 AU 581819 B2 AU581819 B2 AU 581819B2 AU 76771/87 A AU76771/87 A AU 76771/87A AU 7677187 A AU7677187 A AU 7677187A AU 581819 B2 AU581819 B2 AU 581819B2
Authority
AU
Australia
Prior art keywords
human
animal cells
immortalization
cell
animal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU76771/87A
Other versions
AU7677187A (en
Inventor
Hinrich Johann Abken
Heinrich Barchet
Herbert Jungfer
Klaus Willecke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of AU7677187A publication Critical patent/AU7677187A/en
Application granted granted Critical
Publication of AU581819B2 publication Critical patent/AU581819B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Abstract

To immortalise human or animal cells by fusion with a non-replicable fragment of a cell which can be permanently cultured, and cultivation thereof in a culture medium without selection substances, the cell to be immortalised is used to transfect DNA obtained from the cytoplasm of a human or animal cell which can be permanently cultured.
AU76771/87A 1986-08-12 1987-08-11 Process for the immortalization of human and animal cells Ceased AU581819B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19863627326 DE3627326A1 (en) 1986-08-12 1986-08-12 IMMORTALIZATION THROUGH DNS TRANSMISSION
DE3627326 1986-08-12

Publications (2)

Publication Number Publication Date
AU7677187A AU7677187A (en) 1988-02-18
AU581819B2 true AU581819B2 (en) 1989-03-02

Family

ID=6307220

Family Applications (1)

Application Number Title Priority Date Filing Date
AU76771/87A Ceased AU581819B2 (en) 1986-08-12 1987-08-11 Process for the immortalization of human and animal cells

Country Status (7)

Country Link
EP (1) EP0256512B1 (en)
JP (1) JPH074237B2 (en)
AT (1) ATE87653T1 (en)
AU (1) AU581819B2 (en)
CA (1) CA1313832C (en)
DE (2) DE3627326A1 (en)
ZA (1) ZA875912B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ226750A (en) * 1987-10-29 1990-09-26 Amrad Corp Ltd Immortalisation of neural precursor cells by introducing a retrovirus vector containing a myc-oncogene
AU2921289A (en) * 1987-12-09 1989-07-19 Invitron Corporation Human cell life extension
DE4218945A1 (en) * 1992-06-10 1993-12-16 Boehringer Mannheim Gmbh DNA for immortalizing human or animal cells
WO1997000946A1 (en) * 1995-06-23 1997-01-09 Micromet Gmbh Immortalized epithelial tumor cell
JP2002201138A (en) * 2000-12-28 2002-07-16 Japan Tissue Engineering:Kk Neovascularization inhibitor

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3245665A1 (en) * 1982-05-04 1983-11-10 Boehringer Mannheim Gmbh, 6800 Mannheim METHOD FOR PRODUCING PERMANENT ANIMAL AND HUMAN CELL LINES AND THE USE THEREOF
US4652522A (en) * 1983-07-05 1987-03-24 The University Of Pennsylvania Continuous lymphocyte cell lines, their production and use
WO1986003780A1 (en) * 1984-12-21 1986-07-03 Techniclone Research Partners I Method for electrically immortalizing lymphoid cells

Also Published As

Publication number Publication date
EP0256512A3 (en) 1989-12-06
EP0256512A2 (en) 1988-02-24
JPS6356283A (en) 1988-03-10
ATE87653T1 (en) 1993-04-15
DE3785109D1 (en) 1993-05-06
EP0256512B1 (en) 1993-03-31
CA1313832C (en) 1993-02-23
ZA875912B (en) 1988-02-12
DE3627326A1 (en) 1988-02-18
JPH074237B2 (en) 1995-01-25
AU7677187A (en) 1988-02-18

Similar Documents

Publication Publication Date Title
AU560340B2 (en) Rennin from recombinant dna
IL87196A (en) Serum free medium for the growthof insect cells and expressionof products thereby
SG88714A1 (en) Production of proteins using homologous recombination
DE69637656D1 (en) Methods for breeding animal cells
FR2466503B1 (en) METHOD FOR CULTIVATION OF ANIMAL CELLS IN THE FORM OF SUSPENSION AND SINGLE-LAYER CULTURES IN FERMENTATION CONTAINERS
HU886759D0 (en) Culture medium and process for the production of insecticide nematodes in liquid culture
DE3681738D1 (en)
AU551487B2 (en) Culturing human cells in algae extract
JPS5639779A (en) Cell culturing method of hematisis a type virus
AU581819B2 (en) Process for the immortalization of human and animal cells
EP0235113A3 (en) Immortalization of primary cells
DK0485504T3 (en) Process for Preparation of Activated Protein C by Cell Culture
JPS55131387A (en) Cultivation of cell
AU8217487A (en) Process for the cultivation of mammalian cells in a suspension culture, device for carrying out the process, and use for the preparation of proteins
ATE115183T1 (en) CELL GROWING PROCESS.
EP0263659A3 (en) Tissue culture media containing l-carnitine
EP0313009A3 (en) Method of gene amplification for enhancing protein production in cultured animal cells

Legal Events

Date Code Title Description
MK14 Patent ceased section 143(a) (annual fees not paid) or expired