DE112006002091B4 - Durchflusszytometer und Durchflusszytometrie - Google Patents

Durchflusszytometer und Durchflusszytometrie Download PDF

Info

Publication number
DE112006002091B4
DE112006002091B4 DE112006002091.9T DE112006002091T DE112006002091B4 DE 112006002091 B4 DE112006002091 B4 DE 112006002091B4 DE 112006002091 T DE112006002091 T DE 112006002091T DE 112006002091 B4 DE112006002091 B4 DE 112006002091B4
Authority
DE
Germany
Prior art keywords
fluorescence
scattered light
detecting
forward scattered
signals
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE112006002091.9T
Other languages
German (de)
English (en)
Other versions
DE112006002091T8 (de
DE112006002091T5 (de
Inventor
Masahiko Kanda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bay Bioscience KK
Original Assignee
Bay Bioscience KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bay Bioscience KK filed Critical Bay Bioscience KK
Publication of DE112006002091T5 publication Critical patent/DE112006002091T5/de
Publication of DE112006002091T8 publication Critical patent/DE112006002091T8/de
Application granted granted Critical
Publication of DE112006002091B4 publication Critical patent/DE112006002091B4/de
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
    • G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
    • G01N15/1427—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement with the synchronisation of components, a time gate for operation of components, or suppression of particle coincidences
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G01N2015/1413—Hydrodynamic focussing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1438—Using two lasers in succession
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1454—Optical arrangements using phase shift or interference, e.g. for improving contrast
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1477—Multiparameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N2021/4704—Angular selective
    • G01N2021/4707—Forward scatter; Low angle scatter
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G01N2201/06113—Coherent sources; lasers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Dispersion Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE112006002091.9T 2005-08-08 2006-08-02 Durchflusszytometer und Durchflusszytometrie Expired - Fee Related DE112006002091B4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2005229536A JP4756948B2 (ja) 2005-08-08 2005-08-08 フローサイトメータおよびフローサイトメトリ方法
JP2005-229536 2005-08-08
PCT/JP2006/315290 WO2007018087A1 (ja) 2005-08-08 2006-08-02 フローサイトメータおよびフローサイトメトリ方法

Publications (3)

Publication Number Publication Date
DE112006002091T5 DE112006002091T5 (de) 2008-07-10
DE112006002091T8 DE112006002091T8 (de) 2008-10-09
DE112006002091B4 true DE112006002091B4 (de) 2019-02-21

Family

ID=37727274

Family Applications (1)

Application Number Title Priority Date Filing Date
DE112006002091.9T Expired - Fee Related DE112006002091B4 (de) 2005-08-08 2006-08-02 Durchflusszytometer und Durchflusszytometrie

Country Status (4)

Country Link
US (1) US7990525B2 (https=)
JP (1) JP4756948B2 (https=)
DE (1) DE112006002091B4 (https=)
WO (1) WO2007018087A1 (https=)

Families Citing this family (137)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7340957B2 (en) 2004-07-29 2008-03-11 Los Alamos National Security, Llc Ultrasonic analyte concentration and application in flow cytometry
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
JP2009536313A (ja) 2006-01-11 2009-10-08 レインダンス テクノロジーズ， インコーポレイテッド ナノリアクターの形成および制御において使用するマイクロ流体デバイスおよび方法
ATE540750T1 (de) 2006-05-11 2012-01-15 Raindance Technologies Inc Mikrofluidische vorrichtung und verfahren
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US7835000B2 (en) 2006-11-03 2010-11-16 Los Alamos National Security, Llc System and method for measuring particles in a sample stream of a flow cytometer or the like
WO2008105855A2 (en) * 2006-11-13 2008-09-04 University Of Wyoming Apparatus and method for measuring the fluorescence of large multi-cellular organisms
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
ATE538377T1 (de) 2007-04-02 2012-01-15 Acoustic Cytometry Systems Inc Verfahren zur verbesserten analyse von in einem akustischen feld fokussierten zellen und partikeln
US8083068B2 (en) 2007-04-09 2011-12-27 Los Alamos National Security, Llc Apparatus for separating particles utilizing engineered acoustic contrast capture particles
US7837040B2 (en) * 2007-04-09 2010-11-23 Los Alamos National Security, Llc Acoustic concentration of particles in fluid flow
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
EP2522982B1 (en) * 2007-08-15 2015-09-16 Malvern Instruments Limited Broad-Range Spectrometer
JP4509154B2 (ja) * 2007-09-04 2010-07-21 ソニー株式会社 光照射装置、微粒子解析装置及び光照射方法
JP4556975B2 (ja) 2007-09-04 2010-10-06 ソニー株式会社 光照射方法、光照射装置及び微粒子解析装置
JP4525725B2 (ja) 2007-10-18 2010-08-18 ソニー株式会社 光学測定部、光学測定用部材、及びこれらを配設した微小粒子測定装置、並びに微小粒子の光学測定方法
US8263407B2 (en) 2007-10-24 2012-09-11 Los Alamos National Security, Llc Method for non-contact particle manipulation and control of particle spacing along an axis
US8528406B2 (en) 2007-10-24 2013-09-10 Los Alamos National Security, LLP Method for non-contact particle manipulation and control of particle spacing along an axis
JP4389991B2 (ja) 2007-10-26 2009-12-24 ソニー株式会社 微小粒子の光学的測定方法及び光学的測定装置
US8266950B2 (en) 2007-12-19 2012-09-18 Los Alamos National Security, LLP Particle analysis in an acoustic cytometer
JP2009162660A (ja) * 2008-01-08 2009-07-23 Sony Corp 検出方法及び検出装置
WO2009090562A2 (en) 2008-01-15 2009-07-23 Malvern Instruments Ltd Light scattering measurements using simultaneous detection
JP4572973B2 (ja) 2008-06-16 2010-11-04 ソニー株式会社 マイクロチップ及びマイクロチップにおける送流方法
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP2315629B1 (en) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Droplet libraries
WO2011120006A1 (en) * 2010-03-25 2011-09-29 Auantalife, Inc. A Delaware Corporation Detection system for droplet-based assays
JP5487638B2 (ja) 2009-02-17 2014-05-07 ソニー株式会社 微小粒子分取のための装置及びマイクロチップ
JP2010207133A (ja) 2009-03-10 2010-09-24 Sony Corp 細胞分離方法
EP3415235B1 (en) 2009-03-23 2025-11-12 Bio-Rad Laboratories, Inc. Manipulation of microfluidic droplets
DE102009020252B4 (de) * 2009-05-07 2012-01-12 Krohne Optosens Gmbh Vorrichtung zur Messung der Fluoreszenz eines Mediums
JP5304434B2 (ja) 2009-05-21 2013-10-02 ソニー株式会社 微小粒子測定装置
JP5304456B2 (ja) 2009-06-10 2013-10-02 ソニー株式会社 微小粒子測定装置
JP5493486B2 (ja) 2009-06-16 2014-05-14 ソニー株式会社 物質混合装置と物質混合方法
JP5534214B2 (ja) 2009-10-05 2014-06-25 ベイバイオサイエンス株式会社 フローサイトメータおよびフローサイトメトリ方法
CA2777524C (en) * 2009-10-16 2018-06-12 Canadian Blood Services Dual analyzer system for biological fluid
JP2011095181A (ja) * 2009-10-30 2011-05-12 Sysmex Corp 粒子分析装置
JP2011163787A (ja) 2010-02-05 2011-08-25 Sony Corp 微小粒子分析装置及び微小粒子分析方法
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
CA2789425C (en) 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
JP5437148B2 (ja) 2010-04-23 2014-03-12 ベイバイオサイエンス株式会社 フローサイトメータおよびセルソータ
WO2011140153A1 (en) * 2010-05-03 2011-11-10 Propel Labs, Llc Optical combiner for combining multiple laser beams in a flow cytometer
ES2527665T3 (es) * 2010-05-05 2015-01-28 Beckman Coulter Biomedical, Llc Instrumento de diagnóstico y proceso de flujo
JP2011237201A (ja) 2010-05-06 2011-11-24 Sony Corp 微小粒子分取装置、マイクロチップ及びマイクロチップモジュール
DE102010023486A1 (de) * 2010-06-11 2011-12-15 B. Braun Avitum Ag Nachweisvorrichtung und -verfahren
US8384045B2 (en) * 2010-07-01 2013-02-26 Sony Corporation Minute particle analyzing device and method
JP2012026754A (ja) 2010-07-20 2012-02-09 Sony Corp 微小粒子測定装置及び光照射装置
WO2012021587A2 (en) * 2010-08-10 2012-02-16 Rait Securities, Llc System and method for electronic trading of financial instruments
US8570500B2 (en) * 2010-08-20 2013-10-29 Stratedigm, Inc. Detector arrangement for a flow cytometry system
JP2012047464A (ja) 2010-08-24 2012-03-08 Sony Corp 微小粒子測定装置及び光軸補正方法
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
GB2484930B (en) * 2010-10-26 2017-04-26 Uh Ventures Ltd An improved low cost apparatus and method for the detection of a fluid-borne particle
JP5567526B2 (ja) * 2010-11-12 2014-08-06 株式会社日立ハイテクノロジーズ 分析装置および分析方法
JP5481402B2 (ja) * 2011-01-17 2014-04-23 株式会社日立ハイテクノロジーズ 自動分析装置
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
JP5923859B2 (ja) * 2011-03-15 2016-05-25 ソニー株式会社 レーザー照射装置および微小粒子測定装置
EP2510958B2 (en) 2011-04-11 2023-02-15 Fresenius Medical Care Deutschland GmbH Method and apparatus for monitoring a treatment of a patient, preferably for monitoring hemodialysis, hemodiafiltration and/or peritoneal dialysis
EP3709018A1 (en) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Microfluidic apparatus for identifying components of a chemical reaction
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US8976352B2 (en) * 2011-08-30 2015-03-10 Sony Corporation Microparticle analysis apparatus
JP5772425B2 (ja) * 2011-09-13 2015-09-02 ソニー株式会社 微小粒子測定装置
US9242248B2 (en) * 2011-09-16 2016-01-26 The University Of North Carolina At Charlotte Methods and devices for optical sorting of microspheres based on their resonant optical properties
US9841367B2 (en) 2011-09-16 2017-12-12 The University Of North Carolina At Charlotte Methods and devices for optical sorting of microspheres based on their resonant optical properties
US8582100B1 (en) * 2012-06-19 2013-11-12 The United States Of America, As Represented By The Secretary Of The Navy Remote multisensor optical particle monitor for flowing fluid systems
US8654329B2 (en) * 2012-06-19 2014-02-18 The United States Of America, As Represented By The Secretary Of The Navy Parallel multisensor optical particle sensors for flowing fluid systems
US9140648B2 (en) * 2013-03-12 2015-09-22 Ecolab Usa Inc. Fluorometer with multiple detection channels
JP6121319B2 (ja) 2013-03-29 2017-04-26 シスメックス株式会社 粒子測定装置、照射光学系および照射位置調整方法
US9513206B2 (en) 2013-03-29 2016-12-06 Sysmex Corporation Particle measuring apparatus
US9372143B2 (en) * 2013-05-15 2016-06-21 Captl Llc Scanning image flow cytometer
TWI654417B (zh) * 2013-06-03 2019-03-21 巴哈馬商愛克斯崔里斯科技有限公司 粒子偵測系統及相關方法
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
KR20150062675A (ko) * 2013-11-29 2015-06-08 서울바이오시스 주식회사 휴대용 공기 품질 판단 장치
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9726593B2 (en) 2014-02-28 2017-08-08 Life Technologies Corporation Systems, methods, and apparatuses for optical systems in flow cytometers
MX2016011193A (es) 2014-02-28 2016-12-16 Life Technologies Corp Sistemas, metodos y aparatos para sistemas opticos en citometros de flujo.
JP5646095B1 (ja) * 2014-03-14 2014-12-24 キヤノン株式会社 計測装置
ES2885623T3 (es) 2014-04-21 2021-12-14 Becton Dickinson Co Cartucho de sellado de cinta deslizante para sellar de manera ajustable una cámara de manipulación de muestras de un citómetro de flujo
JP6350086B2 (ja) * 2014-08-05 2018-07-04 株式会社島津製作所 光学的検出器
JP6435175B2 (ja) * 2014-12-02 2018-12-05 株式会社堀場エステック 分解検出装置、分解検出方法、分解検出装置用プログラム、濃度測定装置、及び、濃度制御装置
CN107003239B (zh) * 2014-12-10 2022-03-29 赛拓生物科技公司 自触发流式细胞计数仪
WO2016100954A1 (en) 2014-12-19 2016-06-23 Captl Llc Flow cytometry using hydrodynamically planar flow
WO2016105418A1 (en) * 2014-12-24 2016-06-30 Fluidigm Canada Inc. Methods and systems for correlating results of optical and mass cytometry
WO2016118333A1 (en) * 2015-01-21 2016-07-28 Stratedigm, Inc. Systems and methods for detecting a particle
ES2987551T3 (es) * 2015-02-18 2024-11-15 Becton Dickinson Co Sistemas de detección óptica y métodos de uso de los mismos
JP6475069B2 (ja) 2015-04-23 2019-02-27 アズビル株式会社 粒子検出装置及び粒子の検出方法
US10036698B2 (en) 2015-06-19 2018-07-31 Captl Llc Time-sequential cytometry
JP6596987B2 (ja) * 2015-07-02 2019-10-30 富士電機株式会社 粒子計測装置
DE102015111282A1 (de) * 2015-07-13 2017-01-19 Stiftung Caesar Center Of Advanced European Studies And Research Verfahren zum Beobachten eines chemischen und/oder biologischen Vorgangs
KR101681422B1 (ko) 2015-07-31 2016-11-30 가톨릭대학교 산학협력단 다수의 레이저를 사용하는 세포 분석 장치
WO2017027736A1 (en) * 2015-08-12 2017-02-16 Bio-Rad Laboratories, Inc. Multi-spectral filter profiling and quality control for flow cytometry
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
JP6237806B2 (ja) * 2016-03-16 2017-11-29 ソニー株式会社 微小粒子分取装置
JP7130562B2 (ja) * 2016-03-17 2022-09-05 ベクトン・ディキンソン・アンド・カンパニー ハイスループット蛍光フローサイトメーターを用いる細胞選別
CN107202903B (zh) * 2016-03-18 2020-04-10 深圳迈瑞生物医疗电子股份有限公司 样本分析仪及其样本分析方法
US11112345B2 (en) 2016-06-10 2021-09-07 Sony Corporation Microparticle measurement device and cleaning method for microparticle measurement device
WO2017223081A1 (en) 2016-06-22 2017-12-28 DeNovix Inc. Cell counter
CN110121643B (zh) * 2016-07-15 2022-04-19 安捷伦科技有限公司 用于流式细胞仪的光学检测系统、流式细胞仪系统及其使用方法
EP3279635B1 (en) * 2016-08-04 2022-06-01 Malvern Panalytical Limited Method, processor and machine-readable, non-transient storage medium for characterising particles suspended in a fluid dispersant
CN109477785B (zh) 2016-09-13 2022-09-27 贝克顿·迪金森公司 具有光学均衡的流式细胞仪
JP6350626B2 (ja) * 2016-09-29 2018-07-04 ソニー株式会社 データ解析方法
KR101934926B1 (ko) * 2017-01-10 2019-01-03 충남대학교산학협력단 유동 유체 내의 나노입자 거동 예측 방법 및 장치
EP3361232A1 (en) * 2017-02-09 2018-08-15 Koninklijke Philips N.V. Optical particle sensor and sensing method
EP3586104A4 (en) 2017-02-27 2020-12-16 Becton, Dickinson and Company LIGHT DETECTION SYSTEMS AND THEIR OPERATING PROCEDURES
EP3382372A1 (en) 2017-03-27 2018-10-03 Koninklijke Philips N.V. Optical particle sensor and sensing method
US10648909B2 (en) * 2017-05-25 2020-05-12 Abbott Laboratories Methods and systems for assessing flow cell cleanliness
KR101986903B1 (ko) * 2017-09-29 2019-06-07 포항공과대학교 산학협력단 입자의 광학적 관측 및 분석 장치, 그리고 관측 및 분석 방법
WO2019094950A1 (en) * 2017-11-13 2019-05-16 Cornell University A multiplexed diagnostic assay for iron and vitamin a deficiency and methods of use thereof
ES2778148T3 (es) * 2018-01-31 2020-08-07 Sick Engineering Gmbh Aparato de análisis para la determinación de polvo fino
GB201806412D0 (en) * 2018-04-19 2018-06-06 Castrol Ltd Apparatus and method for fluid analysis
JP2019191013A (ja) 2018-04-25 2019-10-31 ソニー株式会社 微小粒子測定装置
US20220062899A1 (en) * 2019-01-18 2022-03-03 Mission Bio, Inc. Method, Apparatus and System to Detect Sub-Particle Flowrate in a Closed System
JP2020143991A (ja) * 2019-03-06 2020-09-10 ソニー株式会社 微小粒子測定装置、微小粒子分取装置、微小粒子測定システム及び微小粒子分取システム
CN109946219A (zh) * 2019-04-12 2019-06-28 广西师范大学 一种流式细胞仪散射光和荧光检测装置及方法
CA3138806A1 (en) 2019-05-22 2020-11-26 Dalia Dhingra Method and apparatus for simultaneous targeted sequencing of dna, rna and protein
WO2021003255A1 (en) 2019-07-01 2021-01-07 Mission Bio Method and apparatus to normalize quantitative readouts in single-cell experiments
DE102020000677B4 (de) 2020-02-01 2023-01-12 Hochschule Anhalt Universaldurchflussmessgerät und Verfahren zur Steuerung eines Universaldurchflussmessgeräts
US11709125B2 (en) * 2020-02-19 2023-07-25 Becton, Dickinson And Company Strobed laser excitation systems and methods of use thereof
WO2021178889A1 (en) * 2020-03-05 2021-09-10 The Trustees Of Columbia University In The City Of New York Three-dimensional dosimetry procedures, methods and devices, and optical ct scanner apparatus which utilizes fiber optic taper for collimated images
EP4143536B1 (en) * 2020-04-29 2025-08-20 Becton, Dickinson and Company Methods for modulation and synchronous detection in a flow cytometer and systems for same
US11885743B2 (en) * 2020-07-22 2024-01-30 Agar Corporation, Inc. Fluorescence and scatter and absorption spectroscopic apparatus with a sapphire tube and method for analyzing inline low level hydrocarbon in a flow medium
EP3943918A1 (en) * 2020-07-22 2022-01-26 Universidade do Minho Device for identification and quantification of phytoplankton, methods and uses thereof
CN112229781A (zh) * 2020-09-07 2021-01-15 桂林电子科技大学 一种改进的流式细胞仪的光谱细分式光纤集散探测装置
CN112147044A (zh) * 2020-09-07 2020-12-29 桂林电子科技大学 用于流式细胞仪的光谱细分式光纤集散探测装置
US11846578B2 (en) * 2020-09-28 2023-12-19 Lighthouse Worldwide Solutions Apparatus and method for characterization of particles
CN116569208A (zh) * 2020-10-07 2023-08-08 贝克顿·迪金森公司 用于处理和显示多通道光谱直方图的方法及其系统
CN214895849U (zh) 2021-01-28 2021-11-26 贝克曼库尔特生物科技（苏州）有限公司 气泡检测装置和样本处理仪
AU2022246174A1 (en) 2021-03-25 2023-09-14 Iovance Biotherapeutics, Inc. Methods and compositions for t-cell coculture potency assays and use with cell therapy products
AU2022322756A1 (en) 2021-08-04 2024-02-08 Becton, Dickinson And Company Light detection systems having first and second light receivers, and methods of use thereof
CN115876672A (zh) * 2021-09-29 2023-03-31 深圳市帝迈生物技术有限公司 检测组件、流式细胞分析仪及其检测方法
CN114152559A (zh) * 2021-12-13 2022-03-08 常州必达科生物科技有限公司 一种光斑的检测方法及检测装置
CN116840129A (zh) * 2022-03-23 2023-10-03 贝克曼库尔特生物科技（苏州）有限公司 用于流式细胞仪的光检测系统和光检测方法
EP4306929A1 (en) * 2022-07-11 2024-01-17 Ecole Polytechnique Fédérale de Lausanne Fluorometer system, fluorescene analysis method, and use thereof
CN116908077B (zh) * 2023-09-08 2023-11-24 赛雷纳（中国）医疗科技有限公司 一种流式细胞仪及其控制方法
WO2025122678A1 (en) * 2023-12-04 2025-06-12 Cytek Biosciences, Inc. Improving broadband signal collection efficiency in flow cytometers and cell sorters
CN120846953B (zh) * 2025-09-22 2026-01-02 中国科学院苏州生物医学工程技术研究所 一种流式分析系统信号处理方法及装置、设备、存储介质

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61173141A (ja) 1985-01-28 1986-08-04 Canon Inc 粒子解析装置
US5760900A (en) 1989-03-18 1998-06-02 Canon Kabushiki Kaisha Method and apparatus for optically measuring specimen
US20010026573A1 (en) 2000-03-31 2001-10-04 Toru Takayama High-powered semiconductor laser array apparatus in which the wavelength and phase of laser beams from semiconductor laser units above can be matched those of laser beams from semiconductor laser units below, manufacturing method of the semiconductor laser array apparatus and multi-wavelength laser emitting apparatus using the semiconductor laser array apparatuses
WO2004051238A1 (ja) 2002-12-03 2004-06-17 Bay Bioscience Kabushiki Kaisha 生物学的粒子の情報を得る装置

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1354193B1 (en) * 2001-01-26 2007-06-06 Biocal Technology, Inc. Optical detection in a multi-channel bio-separation system
US6944322B2 (en) * 2001-03-28 2005-09-13 Visiongate, Inc. Optical tomography of small objects using parallel ray illumination and post-specimen optical magnification
US6995841B2 (en) * 2001-08-28 2006-02-07 Rice University Pulsed-multiline excitation for color-blind fluorescence detection
US6870165B2 (en) * 2001-10-19 2005-03-22 Biocal Technology, Inc. Multi-color multiplexed analysis in a bio-separation system
JP4284031B2 (ja) * 2002-04-24 2009-06-24 シスメックス株式会社 フローサイトメータ
JP4598766B2 (ja) * 2003-04-29 2010-12-15 エス３アイ， エル エル シィ 生物学的微粒子及び非生物学的微粒子を検出し且つ分類するためのマルチスペクトル光学方法及びシステム
JP4304120B2 (ja) * 2004-04-30 2009-07-29 ベイバイオサイエンス株式会社 生物学的粒子をソーティングする装置及び方法
JP4300366B2 (ja) * 2005-03-30 2009-07-22 独立行政法人産業技術総合研究所 蛍光検出方法、蛍光検出用ビーズの作製方法及び蛍光検出用ビーズ
JP4304195B2 (ja) * 2006-06-13 2009-07-29 ベイバイオサイエンス株式会社 生物学的粒子をソーティングする装置及び方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61173141A (ja) 1985-01-28 1986-08-04 Canon Inc 粒子解析装置
US5760900A (en) 1989-03-18 1998-06-02 Canon Kabushiki Kaisha Method and apparatus for optically measuring specimen
US20010026573A1 (en) 2000-03-31 2001-10-04 Toru Takayama High-powered semiconductor laser array apparatus in which the wavelength and phase of laser beams from semiconductor laser units above can be matched those of laser beams from semiconductor laser units below, manufacturing method of the semiconductor laser array apparatus and multi-wavelength laser emitting apparatus using the semiconductor laser array apparatuses
WO2004051238A1 (ja) 2002-12-03 2004-06-17 Bay Bioscience Kabushiki Kaisha 生物学的粒子の情報を得る装置

Also Published As

Publication number Publication date
WO2007018087A1 (ja) 2007-02-15
JP4756948B2 (ja) 2011-08-24
DE112006002091T8 (de) 2008-10-09
US20090122311A1 (en) 2009-05-14
US7990525B2 (en) 2011-08-02
DE112006002091T5 (de) 2008-07-10
JP2007046947A (ja) 2007-02-22

Similar Documents

Publication Publication Date Title
DE112006002091B4 (de) Durchflusszytometer und Durchflusszytometrie
EP2069761B1 (de) Verfahren und vorrichtung zum hochaufgelösten optischen abtasten einer probe
DE69628542T2 (de) Verfahren und vorrichtung zur durchflusscytometrie
DE69118429T2 (de) Verfahren zur Messung einer Spezies unter Verwendung von Fluoreszenzlicht
DE69623523T2 (de) Analysator von fluoreszierenden Teilchen mit Verzögerungsschaltung zur Ermöglichung der Subtraktion von aus unterschiedlichen Orten stammenden Fluoreszenzpulsen
DE4416558C2 (de) Verfahren zum optischen Messen eines Probenpunkts einer Probe und Vorrichtung zur Durchführung des Verfahrens
DE2455870A1 (de) Verfahren und vorrichtung zur klassifizierung biologischer zellen durch fluoreszente strahlung
DE3310665A1 (de) Vorrichtung zur messung von lichtoptischen parametern von partikeln
WO1998009154A1 (de) System zur unterscheidung fluoreszierender molekülgruppen durch zeitaufgelöste fluoreszenzmessung
DE2732272B1 (de) Verfahren und Vorrichtung zur Fluoreszenzanalyse von gefaerbten Partikeln,insbesondere biologischen Zellen
EP1303750A1 (de) Verfahren und vorrichtung zur mehrfarben-2-photonen-fluoreszenz-koinzidenzanalyse
EP1186882A2 (de) Verfahren und Vorrichtung zur Detektion von Fluoreszenzlicht bei der konfokalen Rastermikroskopie
DE69635296T2 (de) Automatisierte optische Ausrichtung mit Hilfe eines galvanometrischen Abtasters
DE69025370T2 (de) Gerät und Verfahren zur Trennung oder Messung von zu untersuchenden Teilchen in einer Flüssigkeitsprobe
DE60108044T2 (de) Laser Mikroskop
EP3084502B1 (de) Mehrfarben-scanning-mikroskop
DE102022107397B4 (de) Vorrichtung und Verfahren zur Geschlechtsbestimmung bei einem befruchteten Vogelei
WO2007036281A1 (de) Laborvorrichtung zur gleichzeitigen durchführung von reaktionen in einer mehrzahl von proben
EP3649501A1 (de) Verfahren zum untersuchen einer flüssigkeit, die wenigstens eine zelle und/oder wenigstens ein partikel enthält
EP4306929A1 (en) Fluorometer system, fluorescene analysis method, and use thereof
DE102010012580A1 (de) Vorrichtung und Verfahren zur zeitaufgelösten Durchflusszytometrie
WO2018100083A1 (de) Lumineszenzdetektoranordnung, fluoreszenzmikroskop und verfahren zum detektieren eines lumineszenzsignals
DE102020200193A1 (de) Anordnung zur optischen Vorkonditionierung einer optisch aktivierbaren biologischen Probe
DE20221635U1 (de) Optisches System zum Anregen und Messen von Fluoreszenz an oder in mit Fluoreszenzfarbstoffen behandelten Proben
EP1926985A1 (de) Verfahren und vorrichtung zum hochaufgelösten optischen abtasten einer probe

Legal Events

Date Code Title Description
8696 Reprint of erroneous front page
R012 Request for examination validly filed

Effective date: 20121116

R016 Response to examination communication
R018 Grant decision by examination section/examining division
R020 Patent grant now final
R119 Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee