DD300886A5 - Clonierung und Expression von mikrobieller Phytase - Google Patents
Clonierung und Expression von mikrobieller Phytase Download PDFInfo
- Publication number
- DD300886A5 DD300886A5 DD344227A DD34422790A DD300886A5 DD 300886 A5 DD300886 A5 DD 300886A5 DD 344227 A DD344227 A DD 344227A DD 34422790 A DD34422790 A DD 34422790A DD 300886 A5 DD300886 A5 DD 300886A5
- Authority
- DD
- German Democratic Republic
- Prior art keywords
- phytase
- protein
- sequence
- peptide
- expression
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- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fodder In General (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Plant Substances (AREA)
Priority Applications (29)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SK4663-90A SK280670B6 (sk) | 1989-09-27 | 1990-09-25 | Vyčistená a izolovaná sekvencia dna, konštrukt, ve |
| CZ19904663A CZ289014B6 (cs) | 1989-09-27 | 1990-09-25 | Vyčiątěná a izolovaná sekvence DNA kódující fungální fytázu, konstrukt pro expresi, vektory a transformované hostitelské buňky a způsob produkce fytázy |
| IL9580390A IL95803A (en) | 1989-09-27 | 1990-09-26 | Cloning and expression of microbial phytase |
| DD344227A DD300886A5 (de) | 1989-09-27 | 1990-09-26 | Clonierung und Expression von mikrobieller Phytase |
| KR1019910700528A KR0159782B1 (ko) | 1989-09-27 | 1990-09-27 | 미생물 피타아제의 클로닝 및 발현 |
| SU4895487A RU2113468C1 (ru) | 1989-09-27 | 1990-09-27 | Днк, кодирующая фитазу aspergillus niger, рекомбинантная плазмидная днк для экспрессии фитазы (варианты), штаммы-продуценты фитазы (варианты), способ получения фитазы и рекомбинантная фитаза aspergillus niger |
| EP96202943A EP0779037A1 (en) | 1989-09-27 | 1990-09-27 | Cloning and expression of microbial phytase |
| DE0420358T DE420358T1 (de) | 1989-09-27 | 1990-09-27 | Klonierung und Expression mikrobieller Phytase. |
| DE69033103T DE69033103T2 (de) | 1989-09-27 | 1990-09-27 | Klonierung und Expression mikrobieller Phytase |
| PT95447A PT95447B (pt) | 1989-09-27 | 1990-09-27 | Processo para a preparacao de adn,construcoes de expressao e vectores que codificam para fitase e de peptideos e proteinas que possuem actividade de fitase |
| JP02513672A JP3105920B2 (ja) | 1989-09-27 | 1990-09-27 | 細菌性フィターゼをコードする配列、及びフィターゼ活性を有するタンパク質 |
| DK90202565T DK0420358T3 (da) | 1989-09-27 | 1990-09-27 | Kloning og ekspression af mikrobiel phytase |
| HU907465A HU215179B (hu) | 1989-09-27 | 1990-09-27 | Eljárás gomba eredetű fitázaktivitással rendelkező polipeptidek rekombináns úton történő előállítására és felhasználására |
| EP90202565A EP0420358B1 (en) | 1989-09-27 | 1990-09-27 | Cloning and expression of microbial phytase |
| NZ235478A NZ235478A (en) | 1989-09-27 | 1990-09-27 | Peptide or protein with phytase activity; dna encoding it; expression constructs; vectors; transformed host cells and animal feeds containing the peptide or protein |
| CN90108977A CN1053013C (zh) | 1989-09-27 | 1990-09-27 | 微生物植酸酶的克隆及表达 |
| ES90202565T ES2072834T3 (es) | 1989-09-27 | 1990-09-27 | Produccion microbiana de fitasa. |
| CA002341081A CA2341081C (en) | 1989-09-27 | 1990-09-27 | Cloning and expression of microbial phytase |
| PCT/NL1990/000140 WO1991005053A1 (en) | 1989-09-27 | 1990-09-27 | Cloning and expression of microbial phytase |
| AU65011/90A AU636673B2 (en) | 1989-09-27 | 1990-09-27 | Cloning and expression of microbial phytase |
| AT90202565T ATE180014T1 (de) | 1989-09-27 | 1990-09-27 | Klonierung und expression mikrobieller phytase |
| CA002042054A CA2042054C (en) | 1989-09-27 | 1990-09-27 | Cloning and expression of microbial phytase |
| NO912011A NO303988B1 (no) | 1989-09-27 | 1991-05-24 | Rekombinant fytase, dyrefôr som inneholder den samt anvendelser av den |
| FI912530A FI104380B (fi) | 1989-09-27 | 1991-05-24 | Mikrobifytaasin kloonaus ja ilmentäminen |
| BG094500A BG60108B2 (bg) | 1989-09-27 | 1991-05-27 | Метод за получаване на микробиална фитаза |
| LVP-93-1302A LV10310B (en) | 1989-09-27 | 1993-12-03 | Cloning and expression of microbial phytase |
| GR990401907T GR3030819T3 (en) | 1989-09-27 | 1999-07-20 | Cloning and expression of microbial phytase |
| FI992420A FI108299B (fi) | 1989-09-27 | 1999-11-10 | Mikrobifytaasia sisältävä koostumus ja sen käyttö |
| US11/036,272 US20060063243A1 (en) | 1989-09-27 | 2005-01-14 | Cloning and expression of microbial phytase |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP89202436 | 1989-09-27 | ||
| EP90202231 | 1990-08-17 | ||
| DD344227A DD300886A5 (de) | 1989-09-27 | 1990-09-26 | Clonierung und Expression von mikrobieller Phytase |
| EP90202565A EP0420358B1 (en) | 1989-09-27 | 1990-09-27 | Cloning and expression of microbial phytase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DD300886A5 true DD300886A5 (de) | 1992-08-27 |
Family
ID=37728159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DD344227A DD300886A5 (de) | 1989-09-27 | 1990-09-26 | Clonierung und Expression von mikrobieller Phytase |
Country Status (25)
| Country | Link |
|---|---|
| US (1) | US20060063243A1 (cs) |
| EP (2) | EP0779037A1 (cs) |
| JP (1) | JP3105920B2 (cs) |
| KR (1) | KR0159782B1 (cs) |
| CN (1) | CN1053013C (cs) |
| AT (1) | ATE180014T1 (cs) |
| AU (1) | AU636673B2 (cs) |
| BG (1) | BG60108B2 (cs) |
| CA (2) | CA2042054C (cs) |
| CZ (1) | CZ289014B6 (cs) |
| DD (1) | DD300886A5 (cs) |
| DE (2) | DE69033103T2 (cs) |
| DK (1) | DK0420358T3 (cs) |
| ES (1) | ES2072834T3 (cs) |
| FI (2) | FI104380B (cs) |
| GR (1) | GR3030819T3 (cs) |
| HU (1) | HU215179B (cs) |
| IL (1) | IL95803A (cs) |
| LV (1) | LV10310B (cs) |
| NO (1) | NO303988B1 (cs) |
| NZ (1) | NZ235478A (cs) |
| PT (1) | PT95447B (cs) |
| RU (1) | RU2113468C1 (cs) |
| SK (1) | SK280670B6 (cs) |
| WO (1) | WO1991005053A1 (cs) |
Families Citing this family (94)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK122686D0 (da) * | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| US5780292A (en) * | 1987-04-29 | 1998-07-14 | Alko Group Ltd. | Production of phytate degrading enzymes in trichoderma |
| US7705215B1 (en) | 1990-04-17 | 2010-04-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US6803499B1 (en) | 1989-08-09 | 2004-10-12 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US6777589B1 (en) | 1990-01-22 | 2004-08-17 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US6329574B1 (en) | 1990-01-22 | 2001-12-11 | Dekalb Genetics Corporation | High lysine fertile transgenic corn plants |
| HU220773B1 (hu) | 1990-01-22 | 2002-05-28 | Dekalb Genetics Corporation | Eljárás termő transzgenikus kukoricanövények előállítására |
| US5543576A (en) * | 1990-03-23 | 1996-08-06 | Mogen International | Production of enzymes in seeds and their use |
| US5593963A (en) * | 1990-09-21 | 1997-01-14 | Mogen International | Expression of phytase in plants |
| KR100225087B1 (ko) * | 1990-03-23 | 1999-10-15 | 한스 발터라벤 | 피타아제의 식물내 발현 |
| US7033627B2 (en) | 1990-03-23 | 2006-04-25 | Syngenta Mogen B.V. | Production of enzymes in seeds and their use |
| US6395966B1 (en) | 1990-08-09 | 2002-05-28 | Dekalb Genetics Corp. | Fertile transgenic maize plants containing a gene encoding the pat protein |
| ES2131518T3 (es) * | 1991-12-23 | 1999-08-01 | Dsm Nv | Sistema de expresion eucariota. |
| IL104704A0 (en) * | 1992-02-13 | 1993-06-10 | Gist Brocades Nv | Stabilized aqueous liquid formulation of phytase |
| ES2235157T3 (es) * | 1992-07-31 | 2005-07-01 | Ab Enzymes Gmbh | Celulas recombinantes, constructos de adn, vectores y procedimientos para la expresion de enzimas degradantes de fitatos en proporciones deseadas. |
| US6118047A (en) | 1993-08-25 | 2000-09-12 | Dekalb Genetic Corporation | Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction |
| US6326527B1 (en) | 1993-08-25 | 2001-12-04 | Dekalb Genetics Corporation | Method for altering the nutritional content of plant seed |
| CN1064817C (zh) † | 1994-04-22 | 2001-04-25 | 诺沃挪第克公司 | 一种提高植物蛋白溶解性的方法 |
| US6699704B1 (en) | 1994-04-25 | 2004-03-02 | Roche Vitamins Inc. | Heat tolerant phytases |
| US6358722B1 (en) | 1994-04-25 | 2002-03-19 | Roche Vitamins, Inc. | Heat tolerant phytases |
| US6291221B1 (en) | 1994-04-25 | 2001-09-18 | Roche Vitamins Inc. | Heat tolerant phytases |
| DK0684313T3 (da) * | 1994-04-25 | 2006-11-06 | Dsm Ip Assets Bv | Polypeptider med phytaseaktivitet |
| US5830732A (en) * | 1994-07-05 | 1998-11-03 | Mitsui Toatsu Chemicals, Inc. | Phytase |
| US6051431A (en) * | 1994-07-22 | 2000-04-18 | Dsm N.V. | Selection marker gene free recombinant strains: a method for obtaining them and the use of these strains |
| AU716471B2 (en) | 1995-10-13 | 2000-02-24 | Gist-Brocades B.V. | Fungal cellulases |
| AU2077197A (en) * | 1996-03-18 | 1997-10-10 | Novo Nordisk Biotech, Inc. | Polypeptides having phytase activity and nucleic acids encoding same |
| US5985605A (en) * | 1996-06-14 | 1999-11-16 | Her Majesty The Queen In Right Of Canada, As Represented By The Dept. Of Agriculture & Agri-Food Canada | DNA sequences encoding phytases of ruminal microorganisms |
| US5939303A (en) * | 1996-06-14 | 1999-08-17 | Her Majesty The Queen In Right Of Canada, As Represented By The Dept. Of Agriculture & Agri-Food Canada | Phytases of ruminal microorganisms |
| FR2751987B1 (fr) * | 1996-08-01 | 1998-12-31 | Biocem | Phytases de plantes et applications biotechnologiques |
| GB2316082A (en) * | 1996-08-13 | 1998-02-18 | Finnfeeds Int Ltd | Phytase |
| PL332375A1 (en) | 1996-09-25 | 1999-09-13 | Kyowa Hakko Kogyo Kk | Novel phytases and method of obtaining them |
| US6039942A (en) | 1996-12-20 | 2000-03-21 | Novo Nordick A/S | Phytase polypeptides |
| EP0958353B1 (en) * | 1996-12-20 | 2009-03-04 | Novozymes A/S | Phytase polypeptides |
| US6180390B1 (en) | 1997-03-07 | 2001-01-30 | Food Industry Research & Development Institute | Phytase-producing bacteria, phytase and production method of phytase |
| CA2231948C (en) | 1997-03-25 | 2010-05-18 | F. Hoffmann-La Roche Ag | Modified phytases |
| KR100206453B1 (ko) | 1997-03-27 | 1999-07-01 | 박원훈 | 신규한플라즈미드를이용하여형질전환시킨균주 이.채씨오엘아이.피와이로부터생산된피타제 |
| US6432672B1 (en) | 1997-04-11 | 2002-08-13 | Gerardus Cornelis Maria Selten | Gene conversion as a tool for the construction of recombinant industrial filamentous fungi |
| CN100526459C (zh) * | 1997-06-04 | 2009-08-12 | 巴斯福股份公司 | 高活性植酸酶组合物 |
| DE69835815T2 (de) * | 1997-06-04 | 2007-01-11 | Basf Ag | Auf stärke basierte phospatase-granulate |
| TW409035B (en) | 1997-06-04 | 2000-10-21 | Gist Brocades Bv | Starch-based enzyme granulates |
| NZ330940A (en) | 1997-07-24 | 2000-02-28 | F | Production of consensus phytases from fungal origin using computer programmes |
| US6720014B1 (en) * | 1997-08-13 | 2004-04-13 | Diversa Corporation | Phytase-containing foodstuffs and methods of making and using them |
| CN1062309C (zh) * | 1997-12-16 | 2001-02-21 | 中国农业科学院饲料研究所 | 植酸酶及其基因的克隆和表达 |
| CN1309699A (zh) * | 1998-03-23 | 2001-08-22 | 诺维信公司 | 植酸酶变体 |
| US6514495B1 (en) | 1998-03-23 | 2003-02-04 | Novozymes A/S | Phytase varinats |
| JP2002509704A (ja) * | 1998-04-01 | 2002-04-02 | デーエスエム・ナムローゼ・フェンノートシャップ | フィテートの含量が少ない飼料におけるフィターゼの使用 |
| US6451572B1 (en) | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
| US7220542B2 (en) | 2000-07-17 | 2007-05-22 | Van Den Brink Johannes Maarten | Expression cloning in filamentous fungi |
| CA2356642A1 (en) * | 1999-01-22 | 2000-07-27 | Novozymes A/S | Improved phytases |
| US6720174B1 (en) | 1999-01-28 | 2004-04-13 | Novozymes A/S | Phytases |
| EP1165806B1 (en) | 1999-03-31 | 2005-08-03 | Cornell Research Foundation, Inc. | Phosphatases with improved phytase activity |
| ATE369384T1 (de) * | 1999-05-19 | 2007-08-15 | Emd Lexigen Res Ct Corp | Expression und export von interferon-alpha proteinen als fc fusionsproteine |
| AU7405200A (en) | 1999-10-01 | 2001-05-10 | Novozymes A/S | Spray dried enzyme product |
| US6841370B1 (en) | 1999-11-18 | 2005-01-11 | Cornell Research Foundation, Inc. | Site-directed mutagenesis of Escherichia coli phytase |
| KR100377380B1 (ko) * | 2000-07-28 | 2003-03-26 | 대한제당 주식회사 | 파이테이즈 생산 재조합 효모 |
| AU2002334950A1 (en) | 2001-10-26 | 2003-05-12 | Genencor International, Inc. | T. reesei phytase enzymes, polynucleides encoding the enzymes, vectors and host cells thereof, and methods of using |
| AU2002356855A1 (en) | 2001-10-26 | 2003-05-12 | Genencor International, Inc. | Phytase enzymes, nucleic acid sequences encoding phytase enzymes and vectors and host cells incorporating same |
| TWI332822B (en) | 2001-10-31 | 2010-11-11 | Phytex Llc | Phytase-containing animal food and method |
| CN101177675B (zh) | 2002-02-08 | 2014-05-21 | 诺维信公司 | 肌醇六磷酸酶变体 |
| CA2498017C (en) | 2002-09-13 | 2016-06-14 | Cornell Research Foundation, Inc. | Using mutations to improve aspergillus phytases |
| US20040063184A1 (en) | 2002-09-26 | 2004-04-01 | Novozymes North America, Inc. | Fermentation processes and compositions |
| US7579167B2 (en) * | 2002-10-08 | 2009-08-25 | Aventis Pharma S. | Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof |
| WO2004085638A1 (en) * | 2003-03-25 | 2004-10-07 | Republic Of National Fisheries Research And Development Institute | Phytase produced from citrobacter braakii |
| US20040253696A1 (en) | 2003-06-10 | 2004-12-16 | Novozymes North America, Inc. | Fermentation processes and compositions |
| CN1302112C (zh) * | 2003-09-17 | 2007-02-28 | 广东肇庆星湖生物科技股份有限公司 | 利用毕赤酵母生产高比活耐高温植酸酶 |
| DK1804592T3 (da) | 2004-09-27 | 2010-02-01 | Novozymes As | Dampbehandlet, pelleteret fodersammensætning omfattende et granulat, der omfatter en kerne og en coating |
| FR2888249B1 (fr) * | 2005-07-08 | 2007-08-17 | Adisseo France Sas Soc Par Act | Effet synergique de l'association de phytases sur l'hydrolyse de l'acide phytique |
| FR2888250B1 (fr) * | 2005-07-08 | 2007-08-17 | Adisseo France Sas Soc Par Act | Phytase de debaryomyces castellii |
| US7919297B2 (en) | 2006-02-21 | 2011-04-05 | Cornell Research Foundation, Inc. | Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase |
| WO2008017066A2 (en) | 2006-08-03 | 2008-02-07 | Cornell Research Foundation, Inc. | Phytases with improved thermal stability |
| ES2576580T3 (es) | 2006-08-07 | 2016-07-08 | Novozymes A/S | Gránulos de enzima para pienso para animales |
| ES2577430T3 (es) | 2006-08-07 | 2016-07-14 | Novozymes A/S | Gránulos de enzima para pienso para animales |
| US8192734B2 (en) | 2007-07-09 | 2012-06-05 | Cornell University | Compositions and methods for bone strengthening |
| EP2116136A1 (en) | 2008-05-08 | 2009-11-11 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO | Novel phytases |
| WO2011005577A1 (en) | 2009-06-24 | 2011-01-13 | Soparkar Charles N S | Zinc supplementation to increase responsiveness to metalloprotease therapy |
| DK2491120T3 (en) | 2009-10-22 | 2016-03-21 | Basf Se | Synthetic phytasevarianter |
| CN102102094B (zh) * | 2009-12-16 | 2013-03-27 | 福建福大百特科技发展有限公司 | 热稳定脂肪酶,其编码基因的表达及其用途 |
| BR112013027130B1 (pt) | 2011-04-21 | 2021-02-23 | Basf Se | fitase, aditivo de alimentação animal, alimentação animal e usos de uma fitase |
| ES2562654T3 (es) | 2011-04-21 | 2016-03-07 | Basf Se | Variantes sintéticas de fitasa |
| CN102583776B (zh) * | 2012-02-29 | 2013-04-24 | 中国水产科学研究院淡水渔业研究中心 | 一种改良养殖水体环境的复合菌酶制剂及制备方法 |
| WO2014067933A1 (en) | 2012-10-31 | 2014-05-08 | C-Lecta Gmbh | Bioactive carrier preparation for enhanced safety in care products and food |
| CA2923934C (en) | 2013-09-11 | 2023-09-26 | Novozymes A/S | Processes for producing fermentation products |
| CN106455630B (zh) | 2014-06-27 | 2021-08-24 | 帝斯曼知识产权资产管理有限公司 | 改进动物饲料的营养价值的方法 |
| CA3007103A1 (en) | 2015-12-22 | 2017-06-29 | Novozymes A/S | Process of extracting oil from thin stillage |
| CA3070730A1 (en) | 2017-09-15 | 2019-03-21 | Novozymes A/S | Enzyme blends and processes for improving the nutritional quality of animal feed |
| CA3075907A1 (en) | 2017-10-23 | 2019-05-02 | Novozymes A/S | Processes for reducing lactic acid in a biofuel fermentation system |
| EP3707255A1 (en) | 2017-11-09 | 2020-09-16 | Basf Se | Coatings of enzyme particles comprising organic white pigments |
| WO2019231944A2 (en) | 2018-05-31 | 2019-12-05 | Novozymes A/S | Processes for enhancing yeast growth and productivity |
| WO2020160126A1 (en) | 2019-01-31 | 2020-08-06 | Novozymes A/S | Polypeptides having xylanase activity and use thereof for improving the nutritional quality of animal feed |
| EP4009807A1 (en) | 2019-08-05 | 2022-06-15 | Novozymes A/S | Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct |
| MX2022006438A (es) | 2019-12-16 | 2022-06-22 | Novozymes As | Procesos para obtener productos de fermentacion. |
| US20230172233A1 (en) | 2020-05-15 | 2023-06-08 | Dsm Ip Assets B.V. | A method for improving the nutritional value of animal feed |
| CN111849858B (zh) * | 2020-07-20 | 2023-01-10 | 浙江农林大学 | 毛竹原生质体的制备及瞬时转化体系的建立 |
| CN114181952A (zh) * | 2021-11-26 | 2022-03-15 | 中农华威生物制药(湖北)有限公司 | 提高纤维素酶活的基因及在中药饲料添加剂中的应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3297548A (en) * | 1964-07-28 | 1967-01-10 | Int Minerals & Chem Corp | Preparation of acid phytase |
| FI881496L (fi) * | 1987-04-06 | 1988-10-07 | Gist Brocades Nv | Foerfarande foer framstaellning eller extraktion av aminosyror ur dynga. |
| DE3734528A1 (de) * | 1987-10-13 | 1989-05-03 | Hoechst Ag | Verwendung von farbmitteln fuer aufzeichnungsfluessigkeiten |
| UA27702C2 (uk) * | 1989-09-27 | 2000-10-16 | Гіст-Брокейдс Н.В. | Фрагмент геномної днк, що кодує фітазу aspergillus niger,фрагмент кднк, що кодує фітазу aspergillus niger, рекомбінантна плазмідна днк для експресії фітази в aspergillus (варіанти), штам aspergillus-продуцент фітази aspergillus (варіанти), спосіб одержання фітази, рекомбінанта фітаза aspergillus niger |
| US5593963A (en) * | 1990-09-21 | 1997-01-14 | Mogen International | Expression of phytase in plants |
| US6057491A (en) * | 1997-05-29 | 2000-05-02 | Borad Of Regents For University Of Oklahoma | Protein having insecticidal activities and method of use |
-
1990
- 1990-09-25 CZ CZ19904663A patent/CZ289014B6/cs not_active IP Right Cessation
- 1990-09-25 SK SK4663-90A patent/SK280670B6/sk not_active IP Right Cessation
- 1990-09-26 DD DD344227A patent/DD300886A5/de unknown
- 1990-09-26 IL IL9580390A patent/IL95803A/en not_active IP Right Cessation
- 1990-09-27 DE DE69033103T patent/DE69033103T2/de not_active Revoked
- 1990-09-27 DK DK90202565T patent/DK0420358T3/da active
- 1990-09-27 CA CA002042054A patent/CA2042054C/en not_active Expired - Lifetime
- 1990-09-27 NZ NZ235478A patent/NZ235478A/xx unknown
- 1990-09-27 DE DE0420358T patent/DE420358T1/de active Pending
- 1990-09-27 CN CN90108977A patent/CN1053013C/zh not_active Expired - Lifetime
- 1990-09-27 AT AT90202565T patent/ATE180014T1/de not_active IP Right Cessation
- 1990-09-27 KR KR1019910700528A patent/KR0159782B1/ko not_active Expired - Lifetime
- 1990-09-27 EP EP96202943A patent/EP0779037A1/en not_active Ceased
- 1990-09-27 CA CA002341081A patent/CA2341081C/en not_active Expired - Lifetime
- 1990-09-27 RU SU4895487A patent/RU2113468C1/ru active
- 1990-09-27 WO PCT/NL1990/000140 patent/WO1991005053A1/en not_active Ceased
- 1990-09-27 AU AU65011/90A patent/AU636673B2/en not_active Expired
- 1990-09-27 ES ES90202565T patent/ES2072834T3/es not_active Expired - Lifetime
- 1990-09-27 EP EP90202565A patent/EP0420358B1/en not_active Revoked
- 1990-09-27 HU HU907465A patent/HU215179B/hu unknown
- 1990-09-27 JP JP02513672A patent/JP3105920B2/ja not_active Expired - Lifetime
- 1990-09-27 PT PT95447A patent/PT95447B/pt not_active IP Right Cessation
-
1991
- 1991-05-24 NO NO912011A patent/NO303988B1/no not_active IP Right Cessation
- 1991-05-24 FI FI912530A patent/FI104380B/fi active
- 1991-05-27 BG BG094500A patent/BG60108B2/bg unknown
-
1993
- 1993-12-03 LV LVP-93-1302A patent/LV10310B/en unknown
-
1999
- 1999-07-20 GR GR990401907T patent/GR3030819T3/el unknown
- 1999-11-10 FI FI992420A patent/FI108299B/fi active
-
2005
- 2005-01-14 US US11/036,272 patent/US20060063243A1/en not_active Abandoned
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| Date | Code | Title | Description |
|---|---|---|---|
| ASS | Change of applicant or owner |
Owner name: DSM N.V., 6411 TE HEERLEN Effective date: 19990420 |
|
| IF04 | In force in the year 2004 |
Expiry date: 20100927 |