BR9909010A - Variante de fitase, polipeptìdeo de fitase, construção de dna, vetor recombinante de expressão, célula hospedeira, processos para preparar uma variante de fitase, ração ou alimento, para preparar uma ração ou alimento composição, e para reduzir os nìveis de fitato em estrume animal, uso da variante de fitase ou da composição, e planta transgênica ou parte de planta - Google Patents

Variante de fitase, polipeptìdeo de fitase, construção de dna, vetor recombinante de expressão, célula hospedeira, processos para preparar uma variante de fitase, ração ou alimento, para preparar uma ração ou alimento composição, e para reduzir os nìveis de fitato em estrume animal, uso da variante de fitase ou da composição, e planta transgênica ou parte de planta

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Publication number
BR9909010A
BR9909010A BR9909010-4A BR9909010A BR9909010A BR 9909010 A BR9909010 A BR 9909010A BR 9909010 A BR9909010 A BR 9909010A BR 9909010 A BR9909010 A BR 9909010A
Authority
BR
Brazil
Prior art keywords
phytase
food
variant
feed
preparing
Prior art date
Application number
BR9909010-4A
Other languages
English (en)
Inventor
Allan Svendsen
Dirk Kostrewa
Martin Lehmann
Luis Pasamontes
Andrea Tomschv
Adolphus Van Loon
Kurt Vogel
Markus Wyss
Soren Flensted Lassen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of BR9909010A publication Critical patent/BR9909010A/pt

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14Pretreatment of feeding-stuffs with enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)

Abstract

"VARIANTE DE FITASE, POLIPEPTìDEO DE FITASE, CONSTRUçãO DE DNA, VETOR RECOMBINANTE DE EXPRESSãO, CéLULA HOSPEDEIRA, PROCESSOS PARA PREPARAR UMA VARIANTE DE FITASE, RAçãO OU ALIMENTO, PARA PREPARAR UMA RAçãO OU ALIMENTO COMPOSIçãO, E PARA REDUZIR OS NìVEIS DE FITATO EM ESTRUME ANIMAL, USO DA VARIANTE DE FITASE OU DA COMPOSIçãO, E, PLANTA TRANSGêNICA OU PARTE DE PLANTA". Variantes de fitase, sua preparação e usos, cujas variantes de fitase, quando alinhadas de acordo com a Figura 1, são emendadas quando comparadas a uma fitase modelo em pelo menos uma de várias posições. As fitases modelo preferidas são fitases de basidiomicetos e ascomycetes, tais como fitase de Peniophora e fitase de Aspergillus. As variantes preferidas de fitase mostram características de atividade emendadas, tais como atividade específica emendada e/ou termoestabilidade emendada.
BR9909010-4A 1998-03-23 1999-03-22 Variante de fitase, polipeptìdeo de fitase, construção de dna, vetor recombinante de expressão, célula hospedeira, processos para preparar uma variante de fitase, ração ou alimento, para preparar uma ração ou alimento composição, e para reduzir os nìveis de fitato em estrume animal, uso da variante de fitase ou da composição, e planta transgênica ou parte de planta BR9909010A (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DK40798 1998-03-23
DK80698 1998-06-19
DKPA199801176 1998-09-18
DKPA199900091 1999-01-22
PCT/DK1999/000153 WO1999049022A1 (en) 1998-03-23 1999-03-22 Phytase variants

Publications (1)

Publication Number Publication Date
BR9909010A true BR9909010A (pt) 2000-11-28

Family

ID=27439247

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9909010-4A BR9909010A (pt) 1998-03-23 1999-03-22 Variante de fitase, polipeptìdeo de fitase, construção de dna, vetor recombinante de expressão, célula hospedeira, processos para preparar uma variante de fitase, ração ou alimento, para preparar uma ração ou alimento composição, e para reduzir os nìveis de fitato em estrume animal, uso da variante de fitase ou da composição, e planta transgênica ou parte de planta

Country Status (11)

Country Link
EP (1) EP1066373B1 (pt)
JP (1) JP2002507412A (pt)
KR (1) KR20010042138A (pt)
CN (1) CN1309699A (pt)
AU (1) AU765477B2 (pt)
BR (1) BR9909010A (pt)
CA (1) CA2323639A1 (pt)
DE (1) DE69940306D1 (pt)
DK (1) DK1066373T3 (pt)
ES (1) ES2321047T3 (pt)
WO (1) WO1999049022A1 (pt)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003203147B2 (en) * 1998-03-23 2008-05-29 Novozymes A/S Phytase variants
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
US6265197B1 (en) 1998-07-02 2001-07-24 Novozymes A/S Krogshoejvej Starch debranching enzymes
CA2332697A1 (en) * 1998-07-02 2000-01-13 Novozymes A/S Starch debranching enzymes
AU781520B2 (en) * 1999-01-22 2005-05-26 Novozymes A/S Improved phytases
US6720174B1 (en) 1999-01-28 2004-04-13 Novozymes A/S Phytases
WO2001025412A1 (en) 1999-10-01 2001-04-12 Novozymes A/S Enzyme granulate
AU2001239205A1 (en) * 2000-03-10 2001-09-17 Novozymes A/S Compositions and methods for producing high yields of heterologous polypeptides in a pichia cell
JP2003000256A (ja) * 2001-06-11 2003-01-07 Ichibiki Kk フィターゼをコードする遺伝子及びそれを用いてのフィターゼの製造方法
US7510831B2 (en) * 2001-10-26 2009-03-31 Genencor International, Inc. Trichoderma reesei phytase enzymes, nucleic acids encoding such phytase enzymes, vectors and host cells incorporating same and methods of making and using same
AU2002356880A1 (en) 2001-10-31 2003-05-12 Phytex, Llc Phytase-containing animal food and method
TWI326708B (en) * 2001-11-21 2010-07-01 Univ Hong Kong Recombinant bacillus phytases and uses thereof
US7238378B2 (en) 2002-02-08 2007-07-03 Novozymes A/S Phytase variants
CN1294266C (zh) * 2002-08-05 2007-01-10 中国农业科学院饲料研究所 在转基因植物中表达耐热植酸酶
CA2498017C (en) * 2002-09-13 2016-06-14 Cornell Research Foundation, Inc. Using mutations to improve aspergillus phytases
US20040063184A1 (en) 2002-09-26 2004-04-01 Novozymes North America, Inc. Fermentation processes and compositions
US20040253696A1 (en) 2003-06-10 2004-12-16 Novozymes North America, Inc. Fermentation processes and compositions
US20070184521A1 (en) * 2003-07-03 2007-08-09 Alissa Jourdan Novel phytase and gene
EP2163161B1 (en) 2004-09-27 2018-08-01 Novozymes A/S Enzyme Granules
GB0422052D0 (en) * 2004-10-04 2004-11-03 Dansico As Enzymes
US7919297B2 (en) 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
ES2387203T3 (es) 2006-04-04 2012-09-17 Novozymes A/S Variantes de fitasa
US8540984B2 (en) 2006-08-03 2013-09-24 Cornell Research Foundation, Inc. Phytases with improved thermal stability
EP2051590B1 (en) 2006-08-07 2016-04-20 Novozymes A/S Enzyme granules for animal feed
CN103168928A (zh) 2006-08-07 2013-06-26 诺维信公司 用于动物饲料的酶团粒
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
EP2116136A1 (en) * 2008-05-08 2009-11-11 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Novel phytases
EP2799531A1 (de) 2013-05-03 2014-11-05 Clariant Produkte (Deutschland) GmbH Einsatz von Phosphatasen zur enzymatischen Entschleimung von Triglyceriden
CN106399273B (zh) * 2013-07-24 2019-09-20 东莞泛亚太生物科技有限公司 具有提升的酶活性的植酸酶
CN105934518A (zh) 2013-09-11 2016-09-07 诺维信公司 用于生产发酵产品的方法
CN105441406A (zh) * 2014-08-05 2016-03-30 北京大学 植酸酶变体
US10689630B2 (en) 2015-12-22 2020-06-23 Novozymes A/S Processes for producing fermentation products
CN105567656A (zh) * 2016-01-04 2016-05-11 昆明爱科特生物科技有限公司 一种植酸酶突变体及其应用
CN106011101B (zh) * 2016-07-06 2019-03-26 中国农业科学院饲料研究所 植酸酶突变体YkAPPA-L162V及其编码基因和应用
BR112020004476A2 (pt) 2017-09-15 2020-09-08 Novozymes A/S processo para produção de um produto de fermentação, e, uso de uma combinação de enzimas
US11326187B2 (en) 2017-10-23 2022-05-10 Novozymes A/S Processes for producing a fermentation product
MX2020012754A (es) 2018-05-31 2021-02-26 Novozymes As Procesos para mejorar el crecimiento y la productividad de la levadura.
CN113226049A (zh) 2018-12-05 2021-08-06 诺维信公司 酶颗粒的用途
EP3918060A1 (en) 2019-01-31 2021-12-08 Novozymes A/S Polypeptides having xylanase activity and use thereof for improving the nutritional quality of animal feed
AR119596A1 (es) 2019-08-05 2021-12-29 Novozymes As Mezclas de enzimas y procesos para producir un ingrediente alimenticio de alto contenido proteico a partir de un subproducto de la vinaza entera
BR112022011634A2 (pt) 2019-12-16 2022-08-30 Novozymes As Processos para produção de um produto de fermentação, para diminuição da quantidade de amido residual presente em um liquefato e para aumento da quantidade de oligossacarídeos de cadeia curta presente em um liquefato
US20230167422A1 (en) * 2020-04-29 2023-06-01 Nanjing Bestzyme Bio-Engineering Co., Ltd. Parent Phytase Variant
CN111662831A (zh) * 2020-06-12 2020-09-15 浙江工业大学 黑曲霉Rha-N1及其应用
CN113203694B (zh) * 2021-04-26 2023-04-07 中国科学院东北地理与农业生态研究所 Msi的湖泊富营养指数遥感估算方法
CN114317488A (zh) * 2021-12-17 2022-04-12 青岛蔚蓝生物集团有限公司 一种比活力提高的植酸酶突变体

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ289014B6 (cs) * 1989-09-27 2001-10-17 Dsm N. V. Vyčiątěná a izolovaná sekvence DNA kódující fungální fytázu, konstrukt pro expresi, vektory a transformované hostitelské buňky a způsob produkce fytázy
KR100225087B1 (ko) * 1990-03-23 1999-10-15 한스 발터라벤 피타아제의 식물내 발현
WO1997035016A1 (en) * 1996-03-18 1997-09-25 Novo Nordisk Biotech Inc Polypeptides having phytase activity and nucleic acids encoding same
US5985605A (en) * 1996-06-14 1999-11-16 Her Majesty The Queen In Right Of Canada, As Represented By The Dept. Of Agriculture & Agri-Food Canada DNA sequences encoding phytases of ruminal microorganisms
CA2231948C (en) * 1997-03-25 2010-05-18 F. Hoffmann-La Roche Ag Modified phytases
NZ330940A (en) * 1997-07-24 2000-02-28 F Production of consensus phytases from fungal origin using computer programmes

Also Published As

Publication number Publication date
AU3326699A (en) 1999-10-18
ES2321047T3 (es) 2009-06-01
JP2002507412A (ja) 2002-03-12
CN1309699A (zh) 2001-08-22
DE69940306D1 (de) 2009-03-05
EP1066373B1 (en) 2009-01-14
EP1066373A1 (en) 2001-01-10
DK1066373T3 (da) 2009-04-20
KR20010042138A (ko) 2001-05-25
WO1999049022A9 (en) 2001-04-05
CA2323639A1 (en) 1999-09-30
AU765477B2 (en) 2003-09-18
WO1999049022A1 (en) 1999-09-30

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Legal Events

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B25A Requested transfer of rights approved

Owner name: NOVOZYMES A/S (DK)

Free format text: TRANSFERIDO DE: NOVO NORDISK A/S

B06A Notification to applicant to reply to the report for non-patentability or inadequacy of the application [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements
B15K Others concerning applications: alteration of classification

Ipc: C12N 15/82 (2006.01), A23K 10/14 (2016.0