CY1112852T1 - Μεθοδος προσδιορισμου της μεθυλιωσης dνα σε δειγματα αιματος ή ουρων - Google Patents

Μεθοδος προσδιορισμου της μεθυλιωσης dνα σε δειγματα αιματος ή ουρων

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Publication number
CY1112852T1
CY1112852T1 CY20121100439T CY121100439T CY1112852T1 CY 1112852 T1 CY1112852 T1 CY 1112852T1 CY 20121100439 T CY20121100439 T CY 20121100439T CY 121100439 T CY121100439 T CY 121100439T CY 1112852 T1 CY1112852 T1 CY 1112852T1
Authority
CY
Cyprus
Prior art keywords
dna
remote sample
methods
compositions
dynahylation
Prior art date
Application number
CY20121100439T
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English (en)
Inventor
Matthias Ballhause
Kurt Berlin
Theo Devos
Dimo Dietrich
Volker Liebenberg
Cathy Lofton-Day
Joe Lograsso
Jennifer Maas
Fabian Model
Matthias Schuster
Andrew Sledziewski
Reimo Tetzner
Original Assignee
Epigenomics Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag filed Critical Epigenomics Ag
Publication of CY1112852T1 publication Critical patent/CY1112852T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/125—Bisulfite(s)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • General Chemical & Material Sciences (AREA)

Abstract

Εκδοχές της παρούσας εφεύρεσης σχετίζονται με συνθέσεις και μεθόδους αποκόμισης θραυσμάτων DΝΑ από ένα εξωτερικό δείγμα. Σε συγκεκριμένες εκδοχές αποκομίζεται ένα εξωτερικό δείγμα που περιέχει DΝΑ, το DΝΑ απομονώνεται από το εξωτερικό δείγμα και το απομονωμένο DΝΑ κατεργάζεται κατά τρόπο που επιτρέπει διαφοροποίηση της μεθυλιωμένης από τη μη μεθυλιωμένη κυτοσίνη. Επιπρόσθετες, συγκεκριμένες υλοποιήσεις παρέχουν συνθέσεις και μεθόδους ανάλυσης της μεθυλίωσης του DΝΑ ενός εξωτερικού δείγματος. Άλλες εκδοχές παρέχουν συνθέσεις και μεθόδους ενίσχυσης όλου του γενώματος DΝΑ κατεργασμένου με όξινο θειώδες.
CY20121100439T 2005-04-15 2012-05-11 Μεθοδος προσδιορισμου της μεθυλιωσης dνα σε δειγματα αιματος ή ουρων CY1112852T1 (el)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US67224205P 2005-04-15 2005-04-15
US67699705P 2005-05-02 2005-05-02
US69752105P 2005-07-08 2005-07-08
US72360205P 2005-10-04 2005-10-04
US78024806P 2006-03-08 2006-03-08
EP06750657A EP1871912B1 (en) 2005-04-15 2006-04-17 Method for determining DNA methylation in blood or urine samples

Publications (1)

Publication Number Publication Date
CY1112852T1 true CY1112852T1 (el) 2016-02-10

Family

ID=36808881

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20121100439T CY1112852T1 (el) 2005-04-15 2012-05-11 Μεθοδος προσδιορισμου της μεθυλιωσης dνα σε δειγματα αιματος ή ουρων

Country Status (13)

Country Link
US (1) US10731215B2 (el)
EP (2) EP2481810A1 (el)
JP (2) JP5133238B2 (el)
AT (1) ATE547537T1 (el)
AU (1) AU2006236363B2 (el)
CA (1) CA2603815C (el)
CY (1) CY1112852T1 (el)
DK (1) DK1871912T3 (el)
ES (1) ES2382746T3 (el)
PL (1) PL1871912T3 (el)
PT (1) PT1871912E (el)
SI (1) SI1871912T1 (el)
WO (1) WO2006113770A1 (el)

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ATE547537T1 (de) 2012-03-15
CA2603815C (en) 2017-09-26
JP5674696B2 (ja) 2015-02-25
SI1871912T1 (sl) 2012-06-29
JP2012105679A (ja) 2012-06-07
PT1871912E (pt) 2012-05-25
CA2603815A1 (en) 2006-10-26
PL1871912T3 (pl) 2012-07-31
ES2382746T3 (es) 2012-06-13
JP2008537888A (ja) 2008-10-02
AU2006236363A8 (en) 2009-03-05
EP1871912B1 (en) 2012-02-29
JP5133238B2 (ja) 2013-01-30
EP1871912A1 (en) 2008-01-02
AU2006236363A1 (en) 2006-10-26
AU2006236363B2 (en) 2012-11-01
EP2481810A1 (en) 2012-08-01
DK1871912T3 (da) 2012-05-14
US10731215B2 (en) 2020-08-04
WO2006113770A1 (en) 2006-10-26
US20110059432A1 (en) 2011-03-10

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