CN205301333U - 柯萨奇病毒A16和肠道病毒71的IgM抗体联合检测装置 - Google Patents

柯萨奇病毒A16和肠道病毒71的IgM抗体联合检测装置 Download PDF

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CN205301333U
CN205301333U CN201521044935.6U CN201521044935U CN205301333U CN 205301333 U CN205301333 U CN 205301333U CN 201521044935 U CN201521044935 U CN 201521044935U CN 205301333 U CN205301333 U CN 205301333U
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赵旻
杨小军
李欣
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Abstract

本实用新型涉及一种柯萨奇病毒A16和肠道病毒71的IgM抗体联合检测装置，属于医疗检测设备领域。由固相有纯化的高特异性柯萨奇病毒A16抗原、肠道病毒71抗原和抗鼠IgG抗体的硝酸纤维素膜、吸附有抗人IgM抗体的玻璃纤维、样品垫、吸水纸等其它辅料粘贴制成。优点在于：结构新颖，特异性强，既能同时检测标本中的CA16和EV71的IgM抗体，又没有增加生产操作的复杂度。可保证免疫胶体金释放完全的基础上，有效的提高了反应的灵敏度，同样的阈值下，还可降低免疫胶体金的用量，节约成本；灵敏度高、特异性强、操作简便省时、实用性强。

Description

柯萨奇病毒A16和肠道病毒71的IgM抗体联合检测装置
技术领域
本实用新型涉及医疗检测设备领域，特别涉及一种柯萨奇病毒A16和肠道病毒71的IgM抗体联合检测装置，可实现柯萨奇病毒A16型(CA16)和肠道病毒71型(EV71)IgM抗体的灵敏、特异、快速检测。
背景技术
手足口病（HFMD）是一种以手足皮肤和口腔黏膜疱疹为主要症状的小儿急性传染病。本病在国外1957年已有报道，1981年中国上海首次报告了手足口病疫情。近年来，EV71病毒的流行在亚太地区呈上升趋势，引发HFMD的肠道病毒有20多种（型），其中柯萨奇病毒A16型（CA16）和肠道病毒71型（EV71）最为常见。通常情况下，CA16感染引起的HFMD在临床症状等方面与EV71感染所引起的HFMD难以区别。不同的是CA16感染所致的患者可引发心肌炎、心包炎等并发症，而EV71感染容易引起无菌性脑膜炎、脑干脑炎及由此引发急性弛缓性瘫痪、肺水肿和出血等严重并发症，死亡率较高。由于患者为婴幼儿，病情发展快，所以更需要早期、及时诊断疾病。疾病的早期和及时诊断，可以准确鉴别两种病毒，并对疾病的诊疗和预后判断有重要的临床意义，因此建立HFMD快速分型检测方法尤为重要。
发明内容
本实用新型目的是提供一种柯萨奇病毒A16和肠道病毒71的IgM抗体联合检测装置，解决了现有技术存在的上述问题。
本实用新型的上述目的是通过以下技术方案实现的：
样品垫1、免疫胶体金玻璃纤维膜2、硝酸纤维素膜3、吸收垫4分别粘贴在塑料板5上，所述硝酸纤维素膜3的两端分别与吸收垫4、免疫胶体金玻璃纤维膜2搭接，所述免疫胶体金玻璃纤维膜2的另一端与样品垫1搭接；所述硝酸纤维素膜3上设置第一检测线T1、第二检测线T2和质控线C，当第一检测线T1上固相有纯化的高特异柯萨奇病毒A16抗原时，第二检测线T2上固相有纯化的高特异性肠道病毒71抗原，质控线C上喷点羊抗鼠IgG多克隆抗体；当第一检测线T1上固相有纯化的高特异肠道病毒71抗原，第二检测线T2上固相有纯化的高特异性柯萨奇病毒A16抗原，质控线C上喷点羊抗鼠IgG多克隆抗体。
所述的第一检测线T1距离硝酸纤维素膜3的上缘3~12mm。
所述的第二检测线T2距离硝酸纤维素膜3的上缘5~15mm。
所述的质控线C距离硝酸纤维素膜3的上缘10~18mm。
本实用新型的有益效果在于：
1、结构新颖，将柯萨奇病毒A16型(CA16)抗原和肠道病毒71型(EV71)抗原包被于硝酸纤维膜膜上，特异性强，既能同时检测标本中柯萨奇病毒A16型(CA16)抗原和肠道病毒71型(EV71)IgM抗体，又没有增加生产操作的复杂度。
2、可保证免疫胶体金释放完全的基础上，有效的提高了反应的灵敏度，同样的阈值下，还可降低免疫胶体金的用量，节约成本。
3、不需要任何特殊仪器设备，检测成本低。
4、灵敏度高、特异性强、操作简便省时，不需要专业人员操作。实用性强。
附图说明
图1是本实用新型的结构示意图。
具体实施方式
样品垫1、免疫胶体金玻璃纤维膜2、硝酸纤维素膜3、吸收垫4分别粘贴在塑料板5上，所述硝酸纤维素膜3的两端分别与吸收垫4、免疫胶体金玻璃纤维膜2搭接，所述免疫胶体金玻璃纤维膜2的另一端与样品垫1搭接；所述硝酸纤维素膜3上设置第一检测线T1、第二检测线T2和质控线C，当第一检测线T1上固相有纯化的高特异柯萨奇病毒A16抗原时，第二检测线T2上固相有纯化的高特异性肠道病毒71抗原，质控线C上喷点羊抗鼠IgG多克隆抗体；当第一检测线T1上固相有纯化的高特异肠道病毒71抗原，第二检测线T2上固相有纯化的高特异性柯萨奇病毒A16抗原，质控线C上喷点羊抗鼠IgG多克隆抗体。
所述的第一检测线T1上固相有纯化的高特异性柯萨奇病毒A16型(CA16)抗原。
所述的第二检测线T2上固相有纯化的高特异性肠道病毒71型(EV71)抗原。
所述的第一检测线T1距离硝酸纤维素膜3的上缘3~15mm。
所述的第二检测线T2距离硝酸纤维素膜3的上缘5~17mm。
所述的质控线C距离硝酸纤维素膜3的上缘10~18mm。

Claims (4)

1.一种柯萨奇病毒A16和肠道病毒71的IgM抗体联合检测装置，其特征在于：样品垫（1）、免疫胶体金玻璃纤维膜（2）、硝酸纤维素膜（3）、吸收垫（4）分别粘贴在塑料板（5）上，所述硝酸纤维素膜（3）的两端分别与吸收垫（4）、免疫胶体金玻璃纤维膜（2）搭接，所述免疫胶体金玻璃纤维膜（2）的另一端与样品垫（1）搭接；所述硝酸纤维素膜（3）上设置第一检测线（T1）、第二检测线（T2）和质控线（C），当第一检测线（T1）上固相有纯化的高特异柯萨奇病毒A16抗原时，第二检测线（T2）上固相有纯化的高特异性肠道病毒71抗原，质控线（C）上喷点羊抗鼠IgG多克隆抗体；当第一检测线（T1）上固相有纯化的高特异肠道病毒71抗原，第二检测线（T2）上固相有纯化的高特异性柯萨奇病毒A16抗原，质控线（C）上喷点羊抗鼠IgG多克隆抗体。
2.根据权利要求1所述的柯萨奇病毒A16和肠道病毒71的IgM抗体联合检测装置，其特征在于：所述的第一检测线（T1）距离硝酸纤维素膜（3）的上缘3~12mm。
3.根据权利要求1所述的柯萨奇病毒A16和肠道病毒71的IgM抗体联合检测装置，其特征在于：所述的第二检测线（T2）距离硝酸纤维素膜（3）的上缘5~15mm。
4.根据权利要求1所述的柯萨奇病毒A16和肠道病毒71的IgM抗体联合检测装置，其特征在于：所述的质控线（C）距离硝酸纤维素膜（3）的上缘10~18mm。
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