CN115279925A - 新型冠状病毒双重检测试剂盒 - Google Patents

新型冠状病毒双重检测试剂盒 Download PDF

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CN115279925A
CN115279925A CN202080095719.1A CN202080095719A CN115279925A CN 115279925 A CN115279925 A CN 115279925A CN 202080095719 A CN202080095719 A CN 202080095719A CN 115279925 A CN115279925 A CN 115279925A
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primer
kit
seq
detection
probe
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蒋析文
范建
彭海龙
彭荣俞
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Guangzhou Da'an Gene Co ltd
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Guangzhou Da'an Gene Co ltd
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12N2770/00011—Details
    • C12N2770/20011—Coronaviridae
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

本发明提供了新型冠状病毒双重检测试剂盒，具体的地，本发明公开了一种多重检测新型冠状病毒核酸的试剂盒及方法，能够同时检测新型冠状病毒的2个核酸靶标，具有极高的灵敏度和特异性，并且显著提高了病毒鉴定的准确性。

Description

PCT国内申请，说明书已公开。

Claims (15)

  1. PCT国内申请，权利要求书已公开。
CN202080095719.1A 2020-02-06 2020-04-10 新型冠状病毒双重检测试剂盒 Pending CN115279925A (zh)

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CN202010078064 2020-02-06
CN2020100780649 2020-02-06
PCT/CN2020/084231 WO2021155638A1 (zh) 2020-02-06 2020-04-10 新型冠状病毒双重检测试剂盒

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CN115279925A true CN115279925A (zh) 2022-11-01

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US (1) US11649511B2 (zh)
EP (1) EP3882364B1 (zh)
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WO (1) WO2021155638A1 (zh)

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CN111808990A (zh) * 2020-06-24 2020-10-23 阿吉安(福州)基因医学检验实验室有限公司 2019-nCoV核酸检测试剂盒
CN111893217A (zh) * 2020-08-18 2020-11-06 上海派森诺生物科技股份有限公司 一种新型冠状病毒组合物及其试剂盒及其检测方法
CN112063752A (zh) * 2020-08-20 2020-12-11 广东省科学院动物研究所 一种通用型冠状病毒pcr引物及其应用
CN112210623A (zh) * 2020-09-10 2021-01-12 合肥金域医学检验实验室有限公司 一种快速筛查SARS-CoV-2病毒核酸的质控体系
CN112458204A (zh) * 2020-11-03 2021-03-09 厦门大学 一种新型冠状病毒核酸检测试剂盒及检测方法
CN112662809A (zh) * 2020-12-02 2021-04-16 湖州市中心医院 一种用于新型冠状病毒covid-19检测的核酸组合物及其应用
CN112553373A (zh) * 2020-12-08 2021-03-26 首都医科大学附属北京朝阳医院 用于新型冠状病毒2019-nCoV核酸快速检测的试剂盒及检测方法
CN112522445A (zh) * 2020-12-23 2021-03-19 中国科学院上海微系统与信息技术研究所 用于新型冠状病毒检测的引物探针组合、试剂盒及方法
CN113481324B (zh) * 2021-07-06 2023-12-05 广州达安基因股份有限公司 检测新型冠状病毒及其d614g突变株的方法和试剂盒
CN113652505A (zh) * 2021-08-13 2021-11-16 广州达安基因股份有限公司 检测新型冠状病毒及其voc-202012/01突变株的方法和试剂盒
CN114350849A (zh) * 2021-12-21 2022-04-15 圣湘生物科技股份有限公司 检测SARS-CoV-2的组合物、试剂盒、方法及其用途
CN114410840A (zh) * 2022-01-10 2022-04-29 广州达安基因股份有限公司 检测新型冠状病毒及其n501y突变位点的试剂盒及检测方法

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CN1450172A (zh) 2003-04-26 2003-10-22 杭州华大基因研发中心 检测非典型性肺炎病毒的方法及其试剂盒
GB2401175A (en) * 2003-05-02 2004-11-03 Hong Kong Dna Chips Ltd Detection of SARS virus by PCR
EP1642978A4 (en) * 2003-06-27 2007-03-21 Eiken Chemical METHOD FOR DETECTING SARS CORONAVIRUS
US20050112558A1 (en) 2003-09-24 2005-05-26 The Chinese University Of Hong Kong Prognostic PCR assay for severe acute respiratory syndrome (SARS)
US20100279273A1 (en) * 2007-07-17 2010-11-04 Universite Laval Nucleic acid sequences for the amplification and detection of respiratory viruses
CN103484565B (zh) 2013-09-02 2016-06-08 湖北朗德医疗科技有限公司 一种实时荧光rt-pcr检测冠状病毒的试剂盒及其应用
CN104846125B (zh) 2015-06-15 2017-10-20 深圳市易瑞生物技术有限公司 一种用于检测mers的荧光rt‑pcr引物、探针和试剂盒及检测方法
CN110468237B (zh) * 2016-03-10 2023-06-30 广州达安基因股份有限公司 检测中东呼吸综合征冠状病毒的引物探针混合液及试剂盒
CN107090519B (zh) 2017-04-05 2020-03-20 苏州协云基因科技有限公司 呼吸道常见病原体多重rt-pcr联合基因芯片检测试剂盒
CN109097499A (zh) * 2018-09-19 2018-12-28 浙江农林大学 一种用于检测pedv-tgev核酸的双重荧光定量rt-pcr检测试剂盒
CN109355437A (zh) * 2018-12-11 2019-02-19 上海捷诺生物科技有限公司 一种呼吸道病原体多重检测试剂盒
CN109576397B (zh) * 2018-12-28 2022-10-14 广州达安基因股份有限公司 一种人类免疫缺陷病毒1型核酸定量检测试剂盒
CN110273026B (zh) 2019-06-20 2022-07-05 广州达安基因股份有限公司 呼吸道感染多重检测试剂盒及检测方法
WO2021152181A1 (en) 2020-01-30 2021-08-05 Biomedrex Ab Virus therapy

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WO2021155638A1 (zh) 2021-08-12
US11649511B2 (en) 2023-05-16
EP3882364B1 (en) 2023-08-23
CN111304369A (zh) 2020-06-19
EP3882364A4 (en) 2022-01-05
EP3882364A1 (en) 2021-09-22
CN111304369B (zh) 2023-06-30
US20210404023A1 (en) 2021-12-30

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