CN110999647A - Cutting propagation method of garlic cloves - Google Patents
Cutting propagation method of garlic cloves Download PDFInfo
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- CN110999647A CN110999647A CN201911305422.9A CN201911305422A CN110999647A CN 110999647 A CN110999647 A CN 110999647A CN 201911305422 A CN201911305422 A CN 201911305422A CN 110999647 A CN110999647 A CN 110999647A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses a cutting propagation method of garlic cloves, which promotes the garlic cloves to root by selecting the types of cutting slips, screening substrates and comprehensively controlling, testing and optimizing the conditions of illumination, temperature, moisture and the like. Collecting annual semi-lignified 1-year and 2-year branches with green stems, regulating and controlling growth hormone of the branches through disinfection and soaking in potassium indolebutyrate solution, cutting the branches in a matrix mixed by coconut chaff and perlite in a volume ratio of 1:1, controlling the temperature to be 20-30 ℃, controlling the humidity to be more than 80-100% and the like, and obtaining vegetative propagation seedlings of the garlic fruits in 3 months. The invention breaks through the problem of vegetative propagation of the garlic, provides a method which is simple in operation, low in cost and easy to popularize, lays a technical foundation for establishing a garlic cutting technical system, screening and breeding excellent germplasm of wild resources of the garlic and breeding new varieties of the garlic, and overcomes the bottleneck that the garlic is developed and utilized from the wild resources to the manpower.
Description
Technical Field
The invention relates to the technical field of plant vegetative propagation, in particular to a cutting propagation method of garlic cloves.
Background
Garlic fruits (Malania oleifera Chun et S.Lee) are the only wiggery plants of allium of Cyperaceae (Olacaceae), are the unique rare species in mountain areas of southeast Yunnan and Cassia karst in China, and are only sporadically distributed in the limestone mountain areas of Guansouth, Funing counties and the West Guangxi province of Yunnan southeast. The garlic fruit is formally named in 1980, and the seed kernel oil is found to be rich in nervonic acid in 1981, so that the great economic development value of the seed kernel oil is preliminarily recognized. The garlic seed kernel is rich in oil up to 51.9-64.5%, and the nervonic acid content in the seed kernel oil is up to 40-67%. Nervonic acid (scientific name: cis-15-tetracosenoic acid) is an ultra-long chain monounsaturated fatty acid, and has good curative effects on recovering nerve ending activity, promoting nerve cell growth and development, improving activity of cranial nerves, preventing encephalasthenia and the like. Nervonic acid has wide application prospect in the aspects of food (nutriment) for infants and children, health care products for adults and treatment medicines for neurological diseases, and is considered as the most promising brain health product in the 21 st century. Because both artificial chemical synthesis and biochemical synthesis of nervonic acid are difficult, the obtaining of nervonic acid resources from animals is limited, and the sustainable obtaining of nervonic acid resources from plants is a main solving way. Among the nervonic acid-containing plants found, the allium sativum seed kernel oil has the highest nervonic acid content, and is the most ideal resource plant for developing nervonic acid.
Due to the huge economic value of the garlic fruits, a very small amount of wild resources are disorderly picked, so that the endangered wild plants are threatened more seriously, and the resources are further exhausted. The number of wild plants of the garlic fruits is small, the fruiting amount is low, the total fruit yield is extremely limited, and artificial large-scale planting for replacing wild resources is a necessary way for reasonably developing and utilizing the garlic fruits. At present, the garlic cloves can only be propagated by seeds, and the transplanting survival rate of the seed seedlings is extremely low. Although the branches of the garlic bulbs can be grafted and propagated, a method of grafting bud stocks (seedling stocks are used as stock stocks) is necessary, and the garlic bulbs are single-species plants and have no stock stocks which can be replaced, so that the current grafting method cannot effectively propagate the garlic bulbs. The tissue culture breeding method is not successful at the present stage. The problem of the propagation of the garlic fruits becomes a bottleneck for the restriction of resource protection and development and utilization. Cutting propagation is commonly used for plants with sexual propagation difficulties or for the cultivation and propagation of plant lines. However, the cuttage propagation of the garlic fruit is extremely difficult, and the Wu-Yan-Jong shows that the cuttage propagation can only grow adventitious buds but has no differentiation of adventitious roots in the preliminary study of the protection of the garlic fruit; the stem tissue of the young tree is adopted to culture and grow callus, and adventitious buds are also differentiated, but the growth speed is slow and the multiplication is difficult. At present, no practical asexual propagation method is available. Therefore, the development of the technical research on the cutting propagation of the garlic cloves can play an important role in the development, utilization and protection of the garlic cloves.
Disclosure of Invention
In order to overcome the technical problems, the invention aims to provide a cutting propagation method of garlic cloves.
In order to achieve the purpose, the invention provides the following technical scheme:
a cutting propagation method of allium sativum fruits comprises the following steps:
s1, selecting cuttage facilities and preparing seedling raising medium
Cuttage is carried out in a greenhouse or a small plastic arched shed of an automatic timing spraying device, and the cuttage matrix is coconut husk and perlite (1: 1);
s2, collecting branches
Selecting annual semi-lignified, 1-year-old and 2-year-old branches with stems green, which germinate on the trunk or at the root and at the periphery of the crown, growing robustly and without diseases and insect pests, wrapping the branches with a wetting material to preserve moisture and prevent water evaporation;
s3, cutting ear preparation and hormone regulation treatment
Pruning the branches collected in the S2 into cutting slips with the length of 10-15 cm, wherein the scion strips contain 2 full buds, the upper ends of the cutting slips are horizontally sheared at the position 2cm above the buds, and the shearing mouths at the lower ends of the cutting slips are positioned below leaves or axillary buds and are sheared into single horse ear-shaped oblique cuts; 2-3 leaves are left in each cutting shoot for photosynthesis, so that nutrients and phytohormones are produced, and the cutting shoots are promoted to take root;
soaking the scion wood in 1000 times of mixed solution of thiediazole copper and azoxystrobin for 30 minutes, and then soaking in 1000mg/L potassium indolebutyrate solution for 30 seconds for cuttage;
s4, cutting method
Vertically inserting the cutting slips into a matrix, controlling the cutting depth within the range of 3-4 cm, wherein if the cutting depth is too deep, the air permeability of the base parts of the cutting slips is poor, the cutting slips are black and rotten, and if the cutting depth is too shallow, the cutting slips are prone to lodging; compacting by hand after cutting to ensure that the cutting slips are tightly contacted with the matrix, and watering the matrix thoroughly after cutting;
s5, controlling temperature, light and fertilizer after cuttage
Controlling the temperature in the shed to be 20-30 ℃, the relative humidity of air to be 80-100 percent,
ventilating and cooling according to weather conditions, reasonably setting spraying time intervals to ensure that the cuttage matrix is free from water shortage and water accumulation, and forming a layer of water film on the surface of the leaf;
making shading measures according to weather conditions; after callus or white root primordium appears, shading and air humidity are reduced, and the callus or white root primordium is irradiated by light before 9 am;
disinfecting 1 time in 1000 times of mixed solution of thiediazole copper and azoxystrobin every 7 days after cuttage; spraying 0.1% of monopotassium phosphate and 0.1% of urea mixed solution every 15 days;
periodically checking whether weeds and plant diseases and insect pests grow on the transplanting bed and taking corresponding measures to prevent and control.
Furthermore, in S1, the coconut coir is desalted coconut coir, and the EC value is determined to be less than 0.5mS/cm, and the coconut coir is washed when the EC value is higher than 0.5 mS/cm.
Further, in S2, cutting branches in cloudy days or in the early morning and evening when sunlight is mild so as to prevent the branches from losing water; completely lignified shoots that are too tender and brown cannot be used.
Furthermore, in S3, branches with diseases and insect pests cannot be used, and the branches are easy to be infected with bacteria and black rot because the humidity during cuttage is above 80%; when the temperature is too tender, the water will be easy to lose and the tea will be wilted and rotten; the brown branches with high lignification degree are too old and have slow rooting and are easy to rot for a long time.
Compared with the prior art, the invention has the following beneficial effects:
1. the cutting propagation method of the garlic cloves solves the problem that the garlic cloves do not root in vegetative propagation. Rooting can be realized 3 months after cuttage, the rooting rate reaches more than 60% after 50 days, the rooting number reaches more than 6, the root system is healthy and strong, and the transplanting survival rate is high. The invention is easy to operate and convenient for technical popularization.
2. The cutting propagation method of the garlic cloves provided by the invention lays a foundation for exploring and fixing excellent plant characters from wild garlic clove resources, establishing excellent strains of the garlic cloves, and breeding new varieties, artificial large-scale breeding and planting the garlic cloves. Overcomes the technical difficulty of vegetative propagation of the garlic and provides a key technical support for the industrialized development of the garlic.
Drawings
FIG. 1 shows the root growth of the cuttings obtained after 30 days of cutting according to the present invention;
FIG. 2 shows the current plant growth condition after 3 months of cutting according to the present invention;
FIG. 3 shows the root growth of the non-woven fabric bag and 5 months after cuttage.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The invention aims to solve the problems that the seed propagation of the existing garlic is limited, the vegetative propagation is difficult, and the protection and the industrial development of the garlic urgently need the vegetative propagation technology. The technical key of the cutting propagation of the garlic cloves is explored, and the cutting propagation method of the garlic cloves is provided, so that technical support is provided for the protection and reasonable utilization of rare plant garlic cloves. The invention adopts the following technical scheme:
(1) cuttage facility selection
According to the requirement of production scale, cuttage is carried out in a greenhouse or a small plastic arched shed of an automatic timing spraying device.
(2) The seedling raising medium is prepared by cutting medium coconut husk and perlite (1: 1).
Coconut coir is produced by processing coconut husk fiber, the processing place is generally at seaside, seawater is used during cleaning, the salt content is high, and roots are burned, so the EC value is noticed during purchase. Even if the coconut coir product is labeled as desalted, it is determined that the EC value is <0.5mS/cm, and that the washing is carried out at a value higher than 0.5 mS/cm. The coconut husk is placed into a snake skin bag and washed with clear water.
(3) And (4) branch collection, namely cutting branches in cloudy days or in the early morning and at the evening when sunlight is mild so as to prevent the branches from losing water. The annual semi-lignified, 1-year-old and 2-year-old branches with green stems are selected to grow strongly, have no diseases and insect pests and cannot be used for the completely lignified branches which are too tender and have brown color. Wrapping with moistening material to keep moisture and prevent water evaporation.
(4) Preparing cutting slips and regulating hormone: trimming the cut into cutting slips with the length of 10-15 cm, wherein each cutting slip contains 2 full buds, the upper ends of the cutting slips are horizontally trimmed at the positions 2cm above the buds, and the cutting openings at the lower ends of the cutting slips are positioned below leaves or axillary buds and are trimmed into single horse ear-shaped oblique cuts;
2-3 leaves are left in each cutting shoot for photosynthesis, so that nutrients and phytohormones are produced, and rooting of the cutting shoots is promoted.
Branches with diseases and insect pests cannot be used, and the branches are easy to be infected with bacteria and black rot due to the fact that the humidity of the branches is above 80% during cuttage; when the temperature is too tender, the water will be easy to lose and the tea will be wilted and rotten; the brown branches with high lignification degree are too old and have slow rooting and are easy to rot for a long time.
And (3) soaking the scion wood in 1000 times of mixed solution of the thiediazole copper and the azoxystrobin for 30 minutes, and then soaking and dipping in 1000mg/L potassium indolebutyrate solution for 30 seconds for cuttage.
(5) The cutting method comprises the step of vertically inserting the cutting slips into a matrix, wherein the cutting depth is controlled within the range of 3-4 cm, the cutting slips are poor in air permeability at the base parts of the cutting slips and prone to black rot when the cutting slips are too deep, and the cutting slips are prone to lodging when the cutting slips are too shallow.
After cutting, the cutting slips are compacted by hands to be tightly contacted with the matrix, and the matrix is thoroughly watered immediately after cutting.
(6) And (3) temperature, light and fertilizer management control after cuttage:
the temperature in the greenhouse is controlled to be 20-30 ℃, and the relative humidity of air is 80% -100%.
Ventilating and cooling according to weather conditions, reasonably setting spraying time intervals to ensure that the cuttage matrix is free from water shortage and water accumulation, and forming a layer of water film on the surface of the leaf;
making shading measures according to weather conditions; after callus or white root primordium appears, shading and air humidity are reduced, and the callus or white root primordium is irradiated by light before 9 am; disinfecting 1 time in 1000 times of mixed solution of thiediazole copper and azoxystrobin every 7 days after cuttage; spraying 0.1% of monopotassium phosphate and 0.1% of urea mixed solution every 15 days;
periodically checking whether weeds and plant diseases and insect pests grow on the transplanting bed and taking corresponding measures to prevent and control.
Example 2
Implementation place of greenhouse of forestry and grassland academy of sciences in Yunnan province
In the morning or at night when the temperature and the illumination are low, selecting annual semi-lignification, 1-year-old, 2-year-old and 3-year-old branch strips on the periphery of a crown of a garlic plant which is strong in growth, free of plant diseases and insect pests and free of mechanical damage as cutting strips, wherein the cutting strips are 0 cm-15 cm long, 3.0-6.0 mm in diameter, flat in upper incision, oblique in lower incision, 1-2 plump buds of each cutting strip, reserving 2-3 leaves, and removing 2/3 leaves.
And (3) soaking the cutting into 1000 times of mixed solution of thiediazole copper and azoxystrobin for 30 minutes, and then soaking and dipping in 500mg/L concentration potassium indolebutyrate for 30 seconds.
3 days before cuttage, a non-woven fabric nutrition bag filled with a matrix of coconut coir and perlite (1:1) and with the specification of 10cmX12cm is used for leaching potassium permanganate with the mass fraction of 0.1% through the matrix for disinfection.
The specific cutting mode is as follows:
vertically inserting the cutting slips into a matrix, wherein the cutting depth is 3cm, compacting after cutting to enable the cutting slips to be tightly connected with the matrix, and immediately watering the seedbed thoroughly after cutting.
The temperature in the greenhouse is controlled to be 20-30 ℃, and the relative humidity of air is 80% -100%.
Reasonably setting a spraying time interval according to weather conditions, ensuring that the cuttage matrix is free from water shortage and water accumulation, and forming a water film on the surface of the leaf;
making shading measures according to weather conditions;
performing regular spot inspection, reducing shading and air humidity after callus or white root primordium appears, and receiving light before 9 am; disinfecting 1 time in 1000 times of mixed solution of thiediazole copper and azoxystrobin every 7 days after cuttage; spraying 0.1% of monopotassium phosphate and 0.1% of urea mixed solution every 15 days;
periodically checking whether weeds, rotten cuttings and plant diseases and insect pests grow on the cutting bed, and taking corresponding measures to prevent and control.
Five months later, the rooting rate of each cutting is shown in table 1.
TABLE 1 rooting percentage for different cuttings
After 3 months of cultivation, the annual, annual or biennial branches have rooted. And (5) observing the rooting condition after 5 months, wherein the rooting rate of the semi-lignified branch in the current year reaches 44.44%.
Example 3
The implementation place is a greenhouse of Yunnan province forestry and grassland academy of sciences in 2019 and 5 months
And further performing tests of different matrix types according to the result of the cutting screening.
Selecting annual semi-lignified or 1-year-old branches at the periphery of a crown of a garlic plant which is strong in growth, free of diseases and insect pests and free of mechanical damage in the morning or evening when the temperature and the illumination are low, cutting 1 cm-15 cm long with the diameter of 3.0-6.0 mm, cutting the upper cut into a flat cut, cutting the lower cut into an oblique cut, keeping 2-3 leaves of 1-2 plump buds of each cutting shoot, and removing 2/3 leaves.
And (3) soaking the cutting into 1000 times of mixed solution of thiediazole copper and azoxystrobin for 30 minutes, and then soaking and dipping in 500mg/L concentration potassium indolebutyrate for 30 seconds.
3 days before cuttage, a non-woven fabric nutrition bag filled with a matrix with the specification of 10cmX12cm is soaked in potassium permanganate with the mass fraction of 0.1% to sterilize the matrix. The substrate types are perlite, river sand, vermiculite, coconut husk, perlite (1:1), soil, vermiculite and perlite (1:1) and 6 types of vermiculite and perlite, and 45 cutting slips are processed each time.
The specific cutting mode is as follows:
vertically inserting the cutting slips into a matrix, wherein the cutting depth is 3cm, compacting after cutting to enable the cutting slips to be tightly connected with the matrix, and immediately watering the seedbed thoroughly after cutting.
The temperature in the greenhouse is controlled to be 20-30 ℃, and the relative humidity of air is 80% -100%.
Reasonably setting a spraying time interval according to weather conditions, ensuring that the cuttage matrix is free from water shortage and water accumulation, and forming a water film on the surface of the leaf;
making shading measures according to weather conditions; after callus or white root primordium appears, shading and air humidity are reduced, and the callus or white root primordium is irradiated by light before 9 am; disinfecting 1 time in 1000 times of mixed solution of thiediazole copper and azoxystrobin every 7 days after cuttage; spraying 0.1% of monopotassium phosphate and 0.1% of urea mixed solution every 15 days;
periodically checking whether weeds, rotten cuttings and plant diseases and insect pests grow on the cutting bed, and taking corresponding measures to prevent and control.
Five months later, the rooting rate of the cuttings with different matrixes is shown in the table 2.
TABLE 2 cuttage rooting rate for different substrates
After 3 months of cultivation, roots had developed. And (5) observing the rooting condition after 5 months, wherein the rooting rate of the matrix is 51.11% of coconut coir and perlite (1: 1).
Example 4
Implementation place of greenhouse of forestry and grassland academy of sciences in Yunnan province
And through cutting and matrix screening, further carrying out tests of different plant growth hormones and concentrations on improving the rooting rate of the cutting.
Selecting annual semi-lignified or 1-year-old branches at the periphery of a crown of a garlic plant which is strong in growth, free of diseases and insect pests and free of mechanical damage in the morning or evening when the illumination is low, wherein cuttings l are 0 cm-15 cm long, the diameter is 3.0-6.0 mm, the upper cuts are flat, the lower cuts are oblique, 1-2 plump buds of each cutting shoot are reserved with 2-3 leaves, and the leaves are removed by 2/3.
The cutting is put into a mixed solution of 1000 times of thiabendazole copper and azoxystrobin for soaking for 30 minutes, and then is put into a 1000mg/L, 500m + g/L and 200mg/L potassium indolebutyrate solution and a 1000mg/L, 500mg/L and 200mg/L sodium naphthaleneacetate solution for dipping for 30 seconds.
3 days before cuttage, a non-woven fabric nutrition bag filled with a matrix of coconut coir and perlite (1:1) and with the specification of 10cmX12cm is used for leaching potassium permanganate with the mass fraction of 0.1% through the matrix for disinfection. 45 cuttings are planted in each treatment.
The specific cutting mode is as follows:
vertically inserting the cutting slips into a matrix, wherein the cutting depth is 3cm, compacting after cutting to enable the cutting slips to be tightly connected with the matrix, and immediately watering the seedbed thoroughly after cutting.
The temperature in the greenhouse is controlled to be 20-30 ℃, and the relative humidity of air is 80% -100%.
Reasonably setting a spraying time interval according to weather conditions, ensuring that the cuttage matrix is free from water shortage and water accumulation, and forming a water film on the surface of the leaf;
making shading measures according to weather conditions; after callus or white root primordium appears, shading and air humidity are reduced, and the callus or white root primordium is irradiated by light before 9 am; disinfecting 1 time in 1000 times of mixed solution of thiediazole copper and azoxystrobin every 7 days after cuttage; spraying 0.1% of monopotassium phosphate and 0.1% of urea mixed solution every 15 days;
periodically checking whether weeds, rotten cuttings and plant diseases and insect pests grow on the cutting bed, and taking corresponding measures to prevent and control.
Five months later, the rooting rate of the cuttings with different matrixes is shown in the table 3.
TABLE 3 rooting rate of different substrate cuttage
And (3) observing the rooting condition after 5 months, wherein the rooting rate reaches 66.67 percent after the seeds are soaked in 1000mg/L potassium indolebutyrate for 30 seconds.
FIG. 1 shows the root growth of the cuttings obtained by 30 days cutting according to the present invention; FIG. 2 shows the current plant growth condition after 3 months of cutting according to the present invention; FIG. 3 shows the root growth of the non-woven fabric bag and 5 months after cuttage.
The invention solves the problem that the garlic fruit does not root in vegetative propagation. Rooting can be realized 3 months after cuttage, the rooting rate reaches more than 60% after 50 days, the rooting number reaches more than 6, the root system is healthy and strong, and the transplanting survival rate is high. The invention is easy to operate and convenient for technical popularization.
The invention lays a foundation for exploring and fixing excellent plant characters from wild garlic fruit resources, establishing an excellent garlic fruit strain, and breeding new varieties, artificial large-scale breeding and planting garlic fruits. Overcomes the technical difficulty of vegetative propagation of the garlic and provides a key technical support for the industrialized development of the garlic.
The garlic fruits are the special tree species in the original Yunnan southeast and Guixi karst mountainous areas and are excellent plants for controlling the stony desertification in the areas. The large-scale planting of the garlic can effectively promote the vegetation recovery and ecological environment protection in the karst region. The origin of the garlic bulbs is also the gathering area of the remote and poor minority of China, and the garlic bulb industry with high economic value can effectively improve the income of local residents and promote the prosperity and stability of frontier areas.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (4)
1. A cutting propagation method of garlic cloves is characterized by comprising the following steps:
s1, selecting cuttage facilities and preparing seedling raising medium
Cuttage is carried out in a greenhouse or a small plastic arched shed of an automatic timing spraying device, and the cuttage matrix is coconut husk and perlite (1: 1);
s2, collecting branches
Selecting annual semi-lignified, 1-year-old and 2-year-old branches with stems green, which germinate on the trunk or at the root and at the periphery of the crown, growing robustly and without diseases and insect pests, wrapping the branches with a wetting material to preserve moisture and prevent water evaporation;
s3, cutting ear preparation and hormone regulation treatment
Pruning the branches collected in the S2 into cutting slips with the length of 10-15 cm, wherein the scion strips contain 2 full buds, the upper ends of the cutting slips are horizontally sheared at the position 2cm above the buds, and the shearing mouths at the lower ends of the cutting slips are positioned below leaves or axillary buds and are sheared into single horse ear-shaped oblique cuts; 2-3 leaves are left in each cutting shoot for photosynthesis, so that nutrients and phytohormones are produced, and the cutting shoots are promoted to take root;
soaking the scion wood in 1000 times of mixed solution of thiediazole copper and azoxystrobin for 30 minutes, and then soaking in 1000mg/L potassium indolebutyrate solution for 30 seconds for cuttage;
s4, cutting method
Vertically inserting the cutting slips into a matrix, controlling the cutting depth within the range of 3-4 cm, wherein if the cutting depth is too deep, the air permeability of the base parts of the cutting slips is poor, the cutting slips are black and rotten, and if the cutting depth is too shallow, the cutting slips are prone to lodging; compacting by hand after cutting to ensure that the cutting slips are tightly contacted with the matrix, and watering the matrix thoroughly after cutting;
s5, controlling temperature, light and fertilizer after cuttage
Controlling the temperature in the shed to be 20-30 ℃, the relative humidity of air to be 80-100 percent,
ventilating and cooling according to weather conditions, reasonably setting spraying time intervals to ensure that the cuttage matrix is free from water shortage and water accumulation, and forming a layer of water film on the surface of the leaf;
making shading measures according to weather conditions; after callus or white root primordium appears, shading and air humidity are reduced, and the callus or white root primordium is irradiated by light before 9 am;
disinfecting 1 time in 1000 times of mixed solution of thiediazole copper and azoxystrobin every 7 days after cuttage; spraying 0.1% of monopotassium phosphate and 0.1% of urea mixed solution every 15 days;
periodically checking whether weeds and plant diseases and insect pests grow on the transplanting bed and taking corresponding measures to prevent and control.
2. The cutting propagation method of the garlic cloves, as claimed in claim 1, wherein in S1, the coconut coir is desalted coconut coir, and the EC value is determined to be less than 0.5mS/cm, and the coconut coir is washed when the EC value is higher than 0.5 mS/cm.
3. The cutting propagation method of the garlic cloves according to claim 1, wherein in S2, the branches are cut in the shade or in the early morning or in the evening when the sunlight is mild, so as to avoid the branches losing water; completely lignified shoots that are too tender and brown cannot be used.
4. The cutting propagation method of the garlic fruits according to claim 1, wherein branches with diseases and insect pests cannot be used in S3, and the humidity during cutting is above 80% and is easy to be infected by bacteria and black rot; when the temperature is too tender, the water will be easy to lose and the tea will be wilted and rotten; the brown branches with high lignification degree are too old and have slow rooting and are easy to rot for a long time.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112154871A (en) * | 2020-09-28 | 2021-01-01 | 广西南药康园投资有限责任公司 | Seedling breeding method of milkweed |
| CN114946512A (en) * | 2022-04-01 | 2022-08-30 | 中国科学院昆明植物研究所 | Cultivation method for improving growth performance of garlic plant |
| CN115053812A (en) * | 2022-07-29 | 2022-09-16 | 右江民族医学院 | Method for establishing garlic fruit regeneration system |
| CN115136801A (en) * | 2022-07-18 | 2022-10-04 | 中国林业科学研究院林业研究所 | Full-illumination spraying cuttage method for ancient arborvitae |
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