CN109705175B - 使用模拟移动床色谱纯化中性人乳寡糖的方法 - Google Patents

使用模拟移动床色谱纯化中性人乳寡糖的方法 Download PDF

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CN109705175B
CN109705175B CN201811603666.0A CN201811603666A CN109705175B CN 109705175 B CN109705175 B CN 109705175B CN 201811603666 A CN201811603666 A CN 201811603666A CN 109705175 B CN109705175 B CN 109705175B
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human milk
lacto
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chromatography
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CN109705175A (zh
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斯特凡·詹尼文
马库斯·海福力克
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Kohansen Breast Milk Oligosaccharides Co ltd
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Kohansen Hmo Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/40Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/70Carbohydrates; Sugars; Derivatives thereof
    • A61K31/702Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00Drugs for disorders of the alimentary tract or the digestive system
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/10Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1814Recycling of the fraction to be distributed
    • B01D15/1821Simulated moving beds
    • B01D15/185Simulated moving beds characterised by the components to be separated
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    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361Ion-exchange
    • B01D15/362Cation-exchange
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/02Reverse osmosis; Hyperfiltration ; Nanofiltration
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/42Electrodialysis; Electro-osmosis ; Electro-ultrafiltration; Membrane capacitive deionization
    • B01D61/422Electrodialysis
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00Processes for the preparation of sugar derivatives
    • C07H1/06Separation; Purification
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00Processes for the preparation of sugar derivatives
    • C07H1/06Separation; Purification
    • C07H1/08Separation; Purification from natural products
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K35/744Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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CN201811603666.0A 2013-10-04 2014-10-02 使用模拟移动床色谱纯化中性人乳寡糖的方法 Active CN109705175B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP13187369.7A EP2857410A1 (en) 2013-10-04 2013-10-04 Process for purification of 2´-fucosyllactose using simulated moving bed chromatography
EP13187369.7 2013-10-04
PCT/EP2014/071145 WO2015049331A1 (en) 2013-10-04 2014-10-02 Process for purification of a neutral human milk oligosaccharide using simulated moving bed chromatography
CN201480054230.4A CN105814070A (zh) 2013-10-04 2014-10-02 使用模拟移动床色谱纯化中性人乳寡糖的方法

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CN109705175B true CN109705175B (zh) 2022-07-05

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US (2) US10435427B2 (enExample)
EP (2) EP2857410A1 (enExample)
JP (1) JP6666243B2 (enExample)
KR (1) KR102298281B1 (enExample)
CN (2) CN109705175B (enExample)
BR (1) BR112016007342B1 (enExample)
DK (1) DK3063159T3 (enExample)
ES (1) ES2868249T3 (enExample)
MX (2) MX373919B (enExample)
PH (1) PH12016500594B1 (enExample)
PL (1) PL3063159T3 (enExample)
RU (1) RU2685537C2 (enExample)
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AU2018295414A1 (en) * 2017-07-07 2020-01-16 Societe Des Produits Nestle S.A. Human milk fortifier
WO2019012461A1 (en) 2017-07-12 2019-01-17 Glycom A/S AMORPHOUS MIXTURE COMPRISING A NEUTRAL MONO- OR OLIGOSACCHARIDE AND A NON-GLUCIDIC ACID COMPONENT
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CN111164090A (zh) * 2017-09-29 2020-05-15 菲仕兰坎皮纳荷兰公司 用于从微生物发酵纯化中性母乳寡糖(hmo)的方法
EP3494805B1 (en) 2017-12-08 2025-08-13 Chr. Hansen HMO GmbH Spray-dried tetrasaccharides
PL3494804T3 (pl) 2017-12-08 2025-10-06 Chr. Hansen HMO GmbH Suszona rozpyłowo 3-fukozylolaktoza
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CN111447845B (zh) * 2017-12-08 2022-10-28 科汉森母乳低聚糖股份有限公司 喷雾干燥的唾液酸乳糖
PL3524067T3 (pl) 2018-02-08 2025-06-09 Chr. Hansen HMO GmbH Suszona rozpyłowo mieszanina oligosacharydów mleka ludzkiego
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KR20210006916A (ko) 2018-05-07 2021-01-19 젠와인 바이오테크놀로지 게엠바하 미생물 발효에 의해 수득된 탄수화물로부터 락토-N-네오테트라오스 (LNnT)를 정제하기 위한 간단한 방법
WO2019226431A1 (en) * 2018-05-23 2019-11-28 Dsm Ip Assets B.V. Production of oligosaccharides
US20210298328A1 (en) * 2018-08-08 2021-09-30 Mars, Incoroprated Pet food products comprising oligosaccharides and methods of use
AU2019362595B2 (en) 2018-10-18 2024-12-19 Basf Se Crystalline form II of 2'-O-fucosyllactose, process for its preparation, nutritional, cosmetic or pharmaceutical formulation containing the same
EP3741770A1 (en) * 2019-05-21 2020-11-25 Jennewein Biotechnologie GmbH Purification of oligosaccharides from a fermentation broth by using filtration
EP3750902A1 (en) * 2019-06-14 2020-12-16 Jennewein Biotechnologie GmbH Process for the purification of lacto-n-neotetraose
WO2021011905A1 (en) * 2019-07-18 2021-01-21 The Regents Of The University Of California Human milk oligosaccharides for control of dietary response and metabolic phenotype
MX2022002949A (es) 2019-09-24 2022-04-06 Prolacta Bioscience Inc Composiciones y metodos para el tratamiento de enfermedades inflamatorias e inmunes.
CN110672756B (zh) * 2019-11-07 2020-12-29 江南大学 一种奶粉中2’-岩藻糖基乳糖含量的检测方法
CN115003681B (zh) * 2020-01-29 2024-07-05 帝斯曼知识产权资产管理有限公司 用于回收和纯化人乳寡糖的方法
KR20220155595A (ko) * 2020-03-20 2022-11-23 글리콤 에이/에스 고체 hmo 생성물을 제공하는 방법, 및 이에 의해 수득된 고체 hmo 생성물
WO2021250191A1 (en) * 2020-06-12 2021-12-16 Basf Se Improved demineralization of fermentation broths and purification of fine chemicals such as oligosaccharides
IL300573A (en) 2020-08-14 2023-04-01 Prolacta Bioscience Inc Breast milk oligosaccharide compositions for use with bacteriotherapy
CN112526022A (zh) * 2020-11-27 2021-03-19 内蒙古伊利实业集团股份有限公司 一种乳中母乳低聚糖的检测方法
JP2024502629A (ja) 2021-01-12 2024-01-22 プロラクタ バイオサイエンス,インコーポレイテッド シンバイオティック治療レジメン
EP4418878A1 (en) * 2021-10-18 2024-08-28 DSM IP Assets B.V. A method for providing an amorphous hmo product by drying
CN117003803A (zh) 2022-05-07 2023-11-07 山东恒鲁生物科技有限公司 一种三糖的新晶型
CN115873051B (zh) 2022-05-17 2024-06-25 山东恒鲁生物科技有限公司 三糖的新晶型
CN115073539B (zh) * 2022-07-21 2023-06-16 南京工业大学 一种2’-岩藻糖基乳糖分离纯化的方法
CN115260255B (zh) * 2022-08-30 2024-06-28 南京工业大学 一种利用模拟移动床分离纯化2’-岩藻糖基乳糖的方法
WO2024130119A2 (en) 2022-12-16 2024-06-20 Prolacta Bioscience, Inc. Synbiotic compositions for short chain fatty acid production
CN115856151B (zh) * 2022-12-27 2025-08-12 深圳市中科朗健生物技术有限公司 一种同时测定多种人乳低聚糖的高效液相色谱检测方法
CN115838390A (zh) * 2022-12-30 2023-03-24 保龄宝生物股份有限公司 一种利用三组分色谱分离技术制备高含量2′-岩藻糖基乳糖的方法
WO2024165630A1 (en) * 2023-02-07 2024-08-15 N.V. Nutricia Nutritional composition with improved segregation resistance
CN118805902A (zh) * 2023-04-19 2024-10-22 科·汉森有限公司 母乳低聚糖
WO2025056571A1 (en) 2023-09-12 2025-03-20 Frieslandcampina Nederland B.V. Milk extracellular vesicle enrichment process
CN117089503B (zh) 2023-10-17 2024-01-02 保龄宝生物股份有限公司 一种大肠杆菌k-12 mg1655 blbyzt6及其应用
WO2025149372A1 (en) 2024-01-09 2025-07-17 Frieslandcampina Nederland B.V. Milk extracellular vesicle enrichment process using peg
CN119192253B (zh) * 2024-09-06 2025-10-21 虹摹生物科技(上海)有限公司 一种岩藻糖基乳糖及其纯化方法
CN120847064B (zh) * 2025-09-23 2025-12-05 吉林省科技创新研究院有限公司 一种液体中微量可见异物分析鉴定方法

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