CN108779448B - α-淀粉酶组合变体 - Google Patents

α-淀粉酶组合变体 Download PDF

Info

Publication number
CN108779448B
CN108779448B CN201680081366.3A CN201680081366A CN108779448B CN 108779448 B CN108779448 B CN 108779448B CN 201680081366 A CN201680081366 A CN 201680081366A CN 108779448 B CN108779448 B CN 108779448B
Authority
CN
China
Prior art keywords
amylase
alpha
starch
enzyme
variant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201680081366.3A
Other languages
English (en)
Chinese (zh)
Other versions
CN108779448A (zh
Inventor
W·奎瓦斯
V·夏尔马
D·E·维尔德斯
李祥揆
D·芬南
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco US Inc filed Critical Danisco US Inc
Publication of CN108779448A publication Critical patent/CN108779448A/zh
Application granted granted Critical
Publication of CN108779448B publication Critical patent/CN108779448B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
CN201680081366.3A 2015-12-09 2016-12-09 α-淀粉酶组合变体 Active CN108779448B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562265301P 2015-12-09 2015-12-09
US62/265,301 2015-12-09
PCT/US2016/066031 WO2017100720A1 (en) 2015-12-09 2016-12-09 Alpha-amylase combinatorial variants

Publications (2)

Publication Number Publication Date
CN108779448A CN108779448A (zh) 2018-11-09
CN108779448B true CN108779448B (zh) 2023-08-18

Family

ID=57799785

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680081366.3A Active CN108779448B (zh) 2015-12-09 2016-12-09 α-淀粉酶组合变体

Country Status (6)

Country Link
US (4) US11920170B2 (https=)
EP (2) EP3387124B1 (https=)
JP (1) JP2019500058A (https=)
CN (1) CN108779448B (https=)
DK (1) DK3387124T3 (https=)
WO (1) WO2017100720A1 (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3387124B1 (en) * 2015-12-09 2021-05-19 Danisco US Inc. Alpha-amylase combinatorial variants
US11879127B2 (en) 2017-08-23 2024-01-23 Danisco Us Inc. Methods and compositions for efficient genetic modifications of Bacillus licheniformis strains
JP2021522799A (ja) * 2018-05-04 2021-09-02 ダニスコ・ユーエス・インク マルトデキストリン及び特殊シロップを生成するための簡素化プロセス
US11541105B2 (en) 2018-06-01 2023-01-03 The Research Foundation For The State University Of New York Compositions and methods for disrupting biofilm formation and maintenance
US12584119B2 (en) * 2018-07-31 2026-03-24 Danisco Us Inc Variant alpha-amylases having amino acid substitutions that lower the PKA of the general acid
WO2020077331A2 (en) * 2018-10-12 2020-04-16 Danisco Us Inc Alpha-amylases with mutations that improve stability in the presence of chelants
CN113302270A (zh) * 2018-12-03 2021-08-24 诺维信公司 低pH粉末洗涤剂组合物
EP3987022A1 (en) * 2019-06-24 2022-04-27 Novozymes A/S Alpha-amylase variants
WO2021030267A1 (en) 2019-08-14 2021-02-18 Danisco Us Inc Compositions and methods for increased protein production in bacillus licheniformis
EP4048683A2 (en) * 2019-10-24 2022-08-31 Danisco US Inc Variant maltopentaose/maltohexaose-forming alpha-amylases
KR20220119607A (ko) * 2019-12-20 2022-08-30 헨켈 아게 운트 코. 카게아아 디스페르신 ix를 포함하는 세정 조성물
WO2021236715A1 (en) * 2020-05-21 2021-11-25 Dupont Nutrition Biosciences Aps Unihibited amylases for brewing with high tannin materials
JP7510309B2 (ja) * 2020-08-31 2024-07-03 花王株式会社 α－アミラーゼ変異体
WO2022071451A1 (ja) * 2020-10-02 2022-04-07 花王株式会社 α－アミラーゼ変異体
WO2023023642A2 (en) * 2021-08-20 2023-02-23 Danisco Us Inc. Methods and compositions for enhanced protein production in bacillus cells
MX2024006004A (es) * 2021-11-18 2024-08-06 Danisco Us Inc Alfa-amilasas de alto rendimiento para la licuefacción de almidón.
JP2023095355A (ja) * 2021-12-24 2023-07-06 花王株式会社 アミラーゼ配合洗浄剤組成物
CN119630280A (zh) 2022-05-14 2025-03-14 诺维信公司 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995034642A1 (en) * 1994-06-15 1995-12-21 Kirin Beer Kabushiki Kaisha Novel transferase and amylase, process for producing the enzymes,use thereof, and gene coding for the same
JPH10262661A (ja) * 1997-03-25 1998-10-06 Cci Corp 新規なα−アミラーゼおよびその単離方法
JP2008000139A (ja) * 1994-06-15 2008-01-10 Kirin Holdings Co Ltd 新規トランスフェラーゼ及びアミラーゼ、それらの製造法及び利用、並びに該新規酵素類の遺伝子
WO2014164800A1 (en) * 2013-03-11 2014-10-09 Danisco Us Inc. Alpha-amylase combinatorial variants
WO2015050723A1 (en) * 2013-10-03 2015-04-09 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
WO2015077126A1 (en) * 2013-11-20 2015-05-28 Danisco Us Inc. Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof
WO2017100720A1 (en) * 2015-12-09 2017-06-15 Danisco Us Inc. Alpha-amylase combinatorial variants

Family Cites Families (186)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
JPS5534046A (en) 1978-09-01 1980-03-10 Cpc International Inc Novel glucoamyrase having excellent heat resistance and production
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
NO840200L (no) 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US4536477A (en) 1983-08-17 1985-08-20 Cpc International Inc. Thermostable glucoamylase and method for its production
US5422267A (en) 1984-05-22 1995-06-06 Robert R. Yocum Industrial yeast comprising an integrated glucoamylase gene
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
US5801038A (en) 1984-05-29 1998-09-01 Genencor International Inc. Modified subtilisins having amino acid alterations
US5972682A (en) 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US4587215A (en) 1984-06-25 1986-05-06 Uop Inc. Highly thermostable amyloglucosidase
US4628031A (en) 1984-09-18 1986-12-09 Michigan Biotechnology Institute Thermostable starch converting enzymes
JPH0697997B2 (ja) 1985-08-09 1994-12-07 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ 新規の酵素的洗浄剤添加物
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
ATE110768T1 (de) 1986-08-29 1994-09-15 Novo Nordisk As Enzymhaltiger reinigungsmittelzusatz.
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
DE3854249T2 (de) 1987-08-28 1996-02-29 Novonordisk As Rekombinante Humicola-Lipase und Verfahren zur Herstellung von rekombinanten Humicola-Lipasen.
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
EP0471265B1 (en) 1988-01-07 1995-10-25 Novo Nordisk A/S Specific protease
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体ｄｎａ、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
WO1989009259A1 (en) 1988-03-24 1989-10-05 Novo-Nordisk A/S A cellulase preparation
US5776757A (en) 1988-03-24 1998-07-07 Novo Nordisk A/S Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof
CA2030554C (en) 1989-06-29 2001-08-28 Wilhelmus J. Quax Mutant microbial .alpha.-amylases with increased thermal, acid, and/or alkaline stability
GB8915658D0 (en) 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
EP0528828B2 (de) 1990-04-14 1997-12-03 Genencor International GmbH Alkalische bacillus-lipasen, hierfür codierende dna-sequenzen sowie bacilli, die diese lipasen produzieren
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
CA2082279C (en) 1990-05-09 2007-09-04 Grethe Rasmussen Cellulase preparation comprising an endoglucanase enzyme
US5162210A (en) 1990-06-29 1992-11-10 Iowa State University Research Foundation Process for enzymatic hydrolysis of starch to glucose
DE69129988T2 (de) 1990-09-13 1999-03-18 Novo Nordisk A/S, Bagsvaerd Lipase-varianten
SK21093A3 (en) 1990-09-28 1993-10-06 Procter & Gamble Polyhydroxy fatty acid amide surfactants to enhace enzyme performance
DE69133035T2 (de) 1991-01-16 2003-02-13 The Procter & Gamble Company, Cincinnati Kompakte Waschmittelzusammensetzungen mit hochaktiven Cellulasen
DK0583420T3 (da) 1991-04-30 1996-07-29 Procter & Gamble Builderholdige flydende detergenter med borsyre-polyol-kompleks til inhibering af proteolytisk enzym
EP0511456A1 (en) 1991-04-30 1992-11-04 The Procter & Gamble Company Liquid detergents with aromatic borate ester to inhibit proteolytic enzyme
KR100258460B1 (ko) 1991-05-01 2000-06-01 한센 핀 베네드 안정화 효소 및 세제 조성물
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
DK72992D0 (da) 1992-06-01 1992-06-01 Novo Nordisk As Enzym
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
EP0651794B1 (en) 1992-07-23 2009-09-30 Novozymes A/S MUTANT $g(a)-AMYLASE, DETERGENT AND DISH WASHING AGENT
EP1431389A3 (en) 1992-10-06 2004-06-30 Novozymes A/S Cellulase variants
US5281526A (en) 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
ATE287946T1 (de) 1993-04-27 2005-02-15 Genencor Int Neuartige lipasevarianten zur verwendung in reinigungsmitteln
DK52393D0 (https=) 1993-05-05 1993-05-05 Novo Nordisk As
DK76893D0 (https=) 1993-06-28 1993-06-28 Novo Nordisk As
US5335791A (en) 1993-08-12 1994-08-09 Simco/Ramic Corporation Backlight sorting system and method
JP2859520B2 (ja) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物
CN1189558C (zh) 1993-10-08 2005-02-16 诺沃奇梅兹有限公司 淀粉酶变体
EP0724631A1 (en) 1993-10-13 1996-08-07 Novo Nordisk A/S H 2?o 2?-stable peroxidase variants
JPH07143883A (ja) 1993-11-24 1995-06-06 Showa Denko Kk リパーゼ遺伝子及び変異体リパーゼ
EP0746618B1 (en) 1994-02-22 2002-08-21 Novozymes A/S A method of preparing a variant of a lipolytic enzyme
ES2364774T3 (es) 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA Enzimas mejoradas y detergentes que las contienen.
US5691295A (en) 1995-01-17 1997-11-25 Cognis Gesellschaft Fuer Biotechnologie Mbh Detergent compositions
WO1995023221A1 (en) 1994-02-24 1995-08-31 Cognis, Inc. Improved enzymes and detergents containing them
WO1995024471A1 (en) 1994-03-08 1995-09-14 Novo Nordisk A/S Novel alkaline cellulases
DK0753057T3 (da) 1994-03-29 2006-01-30 Novozymes As Alkalisk Bacillus-amylase
JP3851656B2 (ja) 1994-05-04 2006-11-29 ジェネンコア インターナショナル インコーポレーテッド 改善された界面活性剤耐性を有するリパーゼ
CA2194571C (en) 1994-06-17 2009-02-24 Jan Metske Van Der Laan Novel amylolytic enzymes derived from the b. licheniformis .alpha.-amylase, having improved characteristics
WO1995035381A1 (en) 1994-06-20 1995-12-28 Unilever N.V. Modified pseudomonas lipases and their use
WO1996000292A1 (en) 1994-06-23 1996-01-04 Unilever N.V. Modified pseudomonas lipases and their use
JPH10504197A (ja) 1994-08-11 1998-04-28 ジェネンコア インターナショナル インコーポレーテッド 改善洗浄用組成物
DE69535733T2 (de) 1994-10-06 2009-04-23 Novozymes A/S Ein enzympräparat mit endoglucanase aktivität
BE1008998A3 (fr) 1994-10-14 1996-10-01 Solvay Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci.
BR9509525A (pt) 1994-10-26 1995-10-26 Novo Nordisk As Construção de dna vetor de expressão recombinante célula processo para produzir a enzima que exibe atividade lipolítica enzima que exibe atividade lipolítica preparação de enzima aditivo de detergente e composição de detergente
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
DK2199378T3 (da) 1995-02-03 2012-10-29 Novozymes As alfa-amylase mutanter
JPH08228778A (ja) 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
EP0815209B2 (en) 1995-03-17 2015-02-25 Novozymes A/S Novel endoglucanases
EP0815193A1 (en) 1995-03-24 1998-01-07 Genencor International Inc. An improved laundry detergent composition comprising amylase
AU6414196A (en) 1995-07-14 1997-02-18 Novo Nordisk A/S A modified enzyme with lipolytic activity
WO1997007202A1 (en) 1995-08-11 1997-02-27 Novo Nordisk A/S Novel lipolytic enzymes
AU6513096A (en) 1995-07-19 1997-02-18 Novo Nordisk A/S Treatment of fabrics
WO1997010342A1 (en) 1995-09-13 1997-03-20 Genencor International, Inc. Alkaliphilic and thermophilic microorganisms and enzymes obtained therefrom
EP0904360B1 (en) 1996-04-30 2013-07-31 Novozymes A/S alpha-AMYLASE MUTANTS
US5763385A (en) 1996-05-14 1998-06-09 Genencor International, Inc. Modified α-amylases having altered calcium binding properties
US6211134B1 (en) 1996-05-14 2001-04-03 Genecor International, Inc. Mutant α-amylase
AU3938997A (en) 1996-08-26 1998-03-19 Novo Nordisk A/S A novel endoglucanase
BR9711479B1 (pt) 1996-09-17 2009-08-11 variante de celulase tendo uma resistência aumentada a tensìdeo de ánion.
CA2265734A1 (en) 1996-10-08 1998-04-16 Novo Nordisk A/S Diaminobenzoic acid derivatives as dye precursors
BR9712473B1 (pt) 1996-11-04 2009-08-11 variantes de subtilase e composições.
US6300116B1 (en) 1996-11-04 2001-10-09 Novozymes A/S Modified protease having improved autoproteolytic stability
AU1461497A (en) 1996-12-09 1998-07-03 Genencor International, Inc. Proteins Having Increased Stability
WO1998034946A1 (en) 1997-02-12 1998-08-13 Massachusetts Institute Of Technology Daxx, a novel fas-binding protein that activates jnk and apoptosis
AU7908898A (en) 1997-07-04 1999-01-25 Novo Nordisk A/S Family 6 endo-1,4-beta-glucanase variants and cleaning composit ions containing them
US6008026A (en) 1997-07-11 1999-12-28 Genencor International, Inc. Mutant α-amylase having introduced therein a disulfide bond
US6080568A (en) 1997-08-19 2000-06-27 Genencor International, Inc. Mutant α-amylase comprising modification at residues corresponding to A210, H405 and/or T412 in Bacillus licheniformis
AR017331A1 (es) 1997-10-13 2001-09-05 Novozymes As Polipeptidos mutantes de alfa-amilasas, aditivo para detergentes y composiciones detergentes que los comprenden.
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
CA2308119C (en) 1997-10-30 2014-06-03 Novo Nordisk A/S .alpha.-amylase mutants
WO1999028448A1 (en) 1997-11-26 1999-06-10 Novo Nordisk A/S Thermostable glucoamylase
AU2207099A (en) 1997-12-24 1999-07-19 Genencor International, Inc. An improved method of assaying for a preferred enzyme and/or preferred detergentcomposition
AU2411699A (en) 1998-02-18 1999-09-06 Novo Nordisk A/S Alkaline bacillus amylase
RU2258739C2 (ru) 1998-02-27 2005-08-20 Новозимс А/С ВАРИАНТ МАЛЬТОГЕННОЙ α-АМИЛАЗЫ
AU761751B2 (en) 1998-02-27 2003-06-12 Novozymes A/S Amylolytic enzyme variants
CA2323068A1 (en) 1998-03-09 1999-09-16 Novo Nordisk A/S Enzymatic preparation of glucose syrup from starch
CA2331340A1 (en) 1998-07-15 2000-01-27 Novozymes A/S Glucoamylase variants
DE19834180A1 (de) 1998-07-29 2000-02-03 Benckiser Nv Zusammensetzung zur Verwendung in einer Geschirrspülmaschine
US6376450B1 (en) 1998-10-23 2002-04-23 Chanchal Kumar Ghosh Cleaning compositions containing multiply-substituted protease variants
US6197565B1 (en) 1998-11-16 2001-03-06 Novo-Nordisk A/S α-Amylase variants
DK2290060T3 (en) 1999-03-30 2017-03-06 Novozymes As The alpha-amylase variants
ATE422538T1 (de) 1999-03-31 2009-02-15 Novozymes As Polypeptide mit alkaliner alpha-amylase-aktivität und für diese kodierende nukleinsäuren
JP4745503B2 (ja) 1999-03-31 2011-08-10 ノボザイムス アクティーゼルスカブ アルカリα−アミラーゼ活性を有するポリペプチド及びそれらをコードする核酸
JP4741128B2 (ja) 1999-08-20 2011-08-03 ノボザイムス アクティーゼルスカブ アルカリバチルスアミラーゼ
US6254645B1 (en) 1999-08-20 2001-07-03 Genencor International, Inc. Enzymatic modification of the surface of a polyester fiber or article
US6933140B1 (en) 1999-11-05 2005-08-23 Genencor International, Inc. Enzymes useful for changing the properties of polyester
JP2003513666A (ja) 1999-11-10 2003-04-15 ノボザイムス アクティーゼルスカブ フンガミル様アルファ−アミラーゼ変異体
WO2001064852A1 (en) 2000-03-03 2001-09-07 Novozymes A/S Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
AU2001240473A1 (en) 2000-03-08 2001-09-17 Novozymes A/S Variants with altered properties
US20030104969A1 (en) 2000-05-11 2003-06-05 Caswell Debra Sue Laundry system having unitized dosing
AU2001258229A1 (en) 2000-05-12 2001-11-26 Novozymes A/S Alpha-amylase variants with altered 1,6-activity
WO2001096537A2 (en) 2000-06-14 2001-12-20 Novozymes A/S Pre-oxidized alpha-amylase
EP1370648A2 (en) 2000-08-01 2003-12-17 Novozymes A/S Alpha-amylase mutants with altered properties
EP1326965A2 (en) 2000-10-13 2003-07-16 Novozymes A/S Alpha-amylase variant with altered properties
EP1516918B2 (en) 2000-11-27 2015-07-01 The Procter & Gamble Company Detergent package
ES2273912T3 (es) 2000-11-27 2007-05-16 THE PROCTER & GAMBLE COMPANY Metodo para lavar vajillas.
EP2159279A3 (en) 2001-05-15 2010-05-12 Novozymes A/S Alpha-amylase variant with altered properties
DE10138753B4 (de) * 2001-08-07 2017-07-20 Henkel Ag & Co. Kgaa Wasch- und Reinigungsmittel mit Hybrid-Alpha-Amylasen
EP1354939A1 (en) 2002-04-19 2003-10-22 The Procter & Gamble Company Pouched cleaning compositions
WO2005003311A2 (en) 2003-06-25 2005-01-13 Novozymes A/S Enzymes for starch processing
ES2425351T3 (es) 2003-06-25 2013-10-14 Novozymes A/S Polipéptidos que tienen actividad de alfa-amilasa y polinucleótidos que codifican los mismos
CA2538349C (en) 2003-06-25 2014-08-12 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
US20080124427A1 (en) 2003-08-22 2008-05-29 Novozymes A/S Process for preparing a dough comprising a starch-degrading glucogenic exo-amy-lase of family 13
CA2534710A1 (en) 2003-08-22 2005-03-03 Novozymes A/S Fungal alpha-amylase variants
EP1516917B1 (en) 2003-09-22 2006-07-26 The Procter & Gamble Company Liquid unit dose detergent composition
US8703459B2 (en) 2003-12-05 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Catalytic domains of beta(1,4)-galactosyltransferase I having altered metal ion specificity
AU2004312117B2 (en) 2004-01-08 2011-09-15 Novozymes A/S Amylase
DE102004020720A1 (de) 2004-04-28 2005-12-01 Henkel Kgaa Verfahren zur Herstellung von Wasch- oder Reinigungsmitteln
US7413887B2 (en) 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
GB0416155D0 (en) 2004-07-20 2004-08-18 Unilever Plc Laundry product
WO2006012902A2 (en) 2004-08-02 2006-02-09 Novozymes A/S Creation of diversity in polypeptides
WO2006012899A1 (en) 2004-08-02 2006-02-09 Novozymes A/S Maltogenic alpha-amylase variants
EP2258837A1 (en) 2004-09-10 2010-12-08 Novozymes North America, Inc. Methods for preventing, removing, reducing, or disrupting biofilm
GB0423986D0 (en) 2004-10-29 2004-12-01 Unilever Plc Method of preparing a laundry product
EP1828379A1 (en) 2004-12-15 2007-09-05 Novozymes A/S Alkaline bacillus amylase
EP1926753B1 (en) 2004-12-22 2010-11-24 Novozymes A/S Hybrid enzymes consisting of an endo-amylase first amino acid sequence and a carbohydrate-binding module as second amino acid sequence
MX2007015471A (es) 2005-06-24 2008-04-04 Novozymes As Amilasas para uso farmaceutico.
KR20080066921A (ko) 2005-10-12 2008-07-17 제넨코 인터내셔날 인코포레이티드 저장-안정성 중성 메탈로프로테아제의 용도 및 제조
EP1996688B1 (en) 2006-03-22 2011-10-26 The Procter & Gamble Company Liquid treatment composition
GB0613069D0 (en) 2006-06-30 2006-08-09 Unilever Plc Laundry articles
CA2655737A1 (en) 2006-06-30 2008-01-03 Novozymes A/S Bacterial alpha-amylase variants
RU2509152C2 (ru) 2006-07-18 2014-03-10 ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН Cпособ мытья посуды
KR20090101193A (ko) 2006-12-21 2009-09-24 다니스코 유에스 인크. 바실러스 종 １９５ 의 알파-아밀라아제 폴리펩티드에 대한 조성물 및 용도
GB0700931D0 (en) 2007-01-18 2007-02-28 Reckitt Benckiser Nv Dosage element and a method of manufacturing a dosage element
EP2121910A1 (en) 2007-02-01 2009-11-25 Novozymes A/S Alpha-amylase and its use
US8021863B2 (en) 2007-02-19 2011-09-20 Novozymes A/S Polypeptides with starch debranching activity
EP2215201A2 (en) 2007-11-05 2010-08-11 Danisco US Inc. Alpha-amylase variants with altered properties
WO2009098229A2 (en) 2008-02-04 2009-08-13 Danisco Us Inc., Genencor Division A method of preparing food products using ts23 alpha-amylase
US8066818B2 (en) 2008-02-08 2011-11-29 The Procter & Gamble Company Water-soluble pouch
EP2088187B2 (en) 2008-02-08 2020-01-22 The Procter and Gamble Company Process For Making A Water-Soluble Pouch
US20090233830A1 (en) 2008-03-14 2009-09-17 Penny Sue Dirr Automatic detergent dishwashing composition
EP2107107A1 (en) 2008-04-02 2009-10-07 The Procter and Gamble Company Water-soluble pouch comprising a detergent composition
US8530216B2 (en) 2008-05-16 2013-09-10 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
BRPI0915606A2 (pt) 2008-06-06 2021-03-09 Danisco Us Inc. Alfa-amilases variantes de bacillus subtilis e métodos de uso das mesmas
CA2726451A1 (en) 2008-06-06 2009-12-10 Danisco Us Inc. Compositions and methods comprising variant microbial proteases
ATE539141T1 (de) 2008-06-13 2012-01-15 Procter & Gamble Beutel mit mehreren kammern
CN102209778B (zh) 2008-11-11 2014-10-15 丹尼斯科美国公司 包含丝氨酸蛋白酶变体的组合物和方法
US8753861B2 (en) 2008-11-11 2014-06-17 Danisco Us Inc. Protease comprising one or more combinable mutations
US20100125046A1 (en) 2008-11-20 2010-05-20 Denome Frank William Cleaning products
CN102292444A (zh) 2008-11-20 2011-12-21 诺维信股份有限公司 具有淀粉分解增强活性的多肽和编码该多肽的多核苷酸
EP2213717B1 (en) 2009-01-28 2017-06-28 The Procter & Gamble Company Laundry multi-compartment pouch composition
WO2010088447A1 (en) 2009-01-30 2010-08-05 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
WO2010091221A1 (en) 2009-02-06 2010-08-12 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
EP2216393B1 (en) 2009-02-09 2024-04-24 The Procter & Gamble Company Detergent composition
BRPI1009263A2 (pt) 2009-03-10 2015-10-06 Danisco Us Inc alfa-amilases relacionadas com cepa de bacillus megaterium dsm90 e métodos de uso das mesmas.
DK2414514T3 (en) 2009-04-01 2015-08-24 Danisco Us Inc FORMATIONS AND PROCEDURES COMPREHENSIVE Alpha- amylase variants WITH CHANGED PROPERTIES
WO2010117511A1 (en) 2009-04-08 2010-10-14 Danisco Us Inc. Halomonas strain wdg195-related alpha-amylases, and methods of use, thereof
GB0906281D0 (en) 2009-04-09 2009-05-20 Reckitt Benckiser Nv Detergent compositions
ES2642318T3 (es) 2009-05-19 2017-11-16 The Procter & Gamble Company Un método para imprimir película soluble en agua
US8728790B2 (en) 2009-12-09 2014-05-20 Danisco Us Inc. Compositions and methods comprising protease variants
WO2011076897A1 (en) 2009-12-22 2011-06-30 Novozymes A/S Use of amylase variants at low temperature
CN102933227A (zh) 2009-12-22 2013-02-13 诺维信公司 包含增强性多肽和淀粉降解酶的组合物及其用途
US20120270267A1 (en) 2010-01-04 2012-10-25 Novozymes A/S Alpha-Amylases
US8905236B2 (en) 2010-01-29 2014-12-09 Monosol, Llc Water-soluble film having improved dissolution and stress properties, and packets made therefrom
US9896673B2 (en) 2010-02-10 2018-02-20 Novozymes A/S Compositions of high stability alpha amylase variants
US20110240510A1 (en) 2010-04-06 2011-10-06 Johan Maurice Theo De Poortere Optimized release of bleaching systems in laundry detergents
EP3095861B1 (en) 2010-05-06 2019-06-26 The Procter and Gamble Company Consumer products with protease variants
EP2576762A4 (en) 2010-06-03 2013-12-04 Mascoma Corp YEAST FOR THE EXPRESSION OF SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOVERIC PROCESSING USING STARCH AND CELLULOSE
ES2527679T5 (es) 2010-06-24 2022-04-19 Procter & Gamble Artículos solubles de dosis unitaria que comprenden un polímero catiónico
PL2609183T3 (pl) 2010-08-23 2019-05-31 Henkel IP & Holding GmbH Kompozycje detergentowe w dawkach jednostkowych i sposoby ich wytwarzania i zastosowania
WO2012059336A1 (en) 2010-11-03 2012-05-10 Henkel Ag & Co. Kgaa Laundry article having cleaning properties
GB201101536D0 (en) 2011-01-31 2011-03-16 Reckitt Benckiser Nv Cleaning article
CN103764823B (zh) 2011-05-05 2018-05-11 丹尼斯科美国公司 包含丝氨酸蛋白酶变体的组合物和方法
RU2014121491A (ru) 2011-10-28 2015-12-10 ДАНИСКО ЮЭс ИНК. Варианты вариантной альфа-амилазы, образующей мальтогексаозу
CN104379737B (zh) 2012-06-08 2018-10-23 丹尼斯科美国公司 对淀粉聚合物具有增强的活性的变体α淀粉酶
JP6474992B2 (ja) 2014-10-21 2019-02-27 浜松ホトニクス株式会社 光検出ユニット

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995034642A1 (en) * 1994-06-15 1995-12-21 Kirin Beer Kabushiki Kaisha Novel transferase and amylase, process for producing the enzymes,use thereof, and gene coding for the same
JP2008000139A (ja) * 1994-06-15 2008-01-10 Kirin Holdings Co Ltd 新規トランスフェラーゼ及びアミラーゼ、それらの製造法及び利用、並びに該新規酵素類の遺伝子
JPH10262661A (ja) * 1997-03-25 1998-10-06 Cci Corp 新規なα−アミラーゼおよびその単離方法
WO2014164800A1 (en) * 2013-03-11 2014-10-09 Danisco Us Inc. Alpha-amylase combinatorial variants
WO2014164834A1 (en) * 2013-03-11 2014-10-09 Danisco Us Inc. Alpha-amylase combinatorial variants
WO2014164777A1 (en) * 2013-03-11 2014-10-09 Danisco Us Inc. Alpha-amylase combinatorial variants
CN105229148A (zh) * 2013-03-11 2016-01-06 丹尼斯科美国公司 α-淀粉酶组合变体
CN105229147A (zh) * 2013-03-11 2016-01-06 丹尼斯科美国公司 α-淀粉酶组合变体
CN110777132A (zh) * 2013-03-11 2020-02-11 丹尼斯科美国公司 α-淀粉酶组合变体
WO2015050723A1 (en) * 2013-10-03 2015-04-09 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
WO2015077126A1 (en) * 2013-11-20 2015-05-28 Danisco Us Inc. Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof
WO2017100720A1 (en) * 2015-12-09 2017-06-15 Danisco Us Inc. Alpha-amylase combinatorial variants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Improving the Thermostability of Raw-Starch-Digesting Amylase from a Cytophaga sp. by Site-Directed Mutagenesis;Rong-Jen Shiau等;《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》;20030430;第69卷(第4期);第2383-2385页 *

Also Published As

Publication number Publication date
EP3901257A1 (en) 2021-10-27
US20260049296A1 (en) 2026-02-19
JP2019500058A (ja) 2019-01-10
US20180163191A1 (en) 2018-06-14
US20190144842A1 (en) 2019-05-16
WO2017100720A1 (en) 2017-06-15
US20240376457A1 (en) 2024-11-14
DK3387124T3 (da) 2021-08-23
BR112018011755A2 (pt) 2018-12-04
EP3387124A1 (en) 2018-10-17
US11920170B2 (en) 2024-03-05
EP3387124B1 (en) 2021-05-19
CN108779448A (zh) 2018-11-09
US12480110B2 (en) 2025-11-25

Similar Documents

Publication Publication Date Title
US12480110B2 (en) Alpha-amylase combinatorial variants
DK202000115Y3 (da) Alfa-amylase-varianter afledt fra alfa-amylasen af Cytophaga sp. amylase (CspAmy2)
CN110662836B (zh) α-淀粉酶组合变体
CN105229147B (zh) α-淀粉酶组合变体
CN104884614A (zh) α-淀粉酶变体
EP2771458A2 (en) Variant maltohexaose-forming alpha-amylase variants
CN105960456A (zh) 对蛋白酶裂解敏感性降低的变体α-淀粉酶及其使用方法
CN104583412A (zh) 采用棒曲霉α-淀粉酶和支链淀粉酶由淀粉制备葡萄糖的方法
EP3060659A1 (en) Alpha-amylases from exiguobacterium, and methods of use, thereof
WO2014099525A1 (en) Paenibacillus curdlanolyticus amylase, and methods of use, thereof
CN104769106A (zh) 使用来自埃默森篮状菌(TALAROMYCES EMERSONII)的α-淀粉酶进行糖化的方法
EP2922951A1 (en) Amylase with maltogenic properties
WO2014200656A1 (en) Alpha-amylase from streptomyces umbrinus
WO2014200658A1 (en) Alpha-amylase from promicromonospora vindobonensis
WO2014200657A1 (en) Alpha-amylase from streptomyces xiamenensis
EP3011020A1 (en) Alpha-amylase from bacillaceae family member
CN104321437A (zh) 使用来自棒曲霉(ASPERGILLUS CLAVATUS)的α-淀粉酶进行糖化
CN104822829A (zh) 具有产麦芽糖特性的淀粉酶
BR122024025098A2 (pt) Variante recombinante de uma alfa-amilase, método para a remoção e composição de detergente
BR112018011755B1 (pt) Variante recombinante de uma alfa-amilase, método para converter amido em oligossacarídeos e composição para liquefazer amido

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant