CN106796175B - 用于探测、检测和分析分子的光学系统和检测芯片 - Google Patents

用于探测、检测和分析分子的光学系统和检测芯片 Download PDF

Info

Publication number
CN106796175B
CN106796175B CN201580054416.4A CN201580054416A CN106796175B CN 106796175 B CN106796175 B CN 106796175B CN 201580054416 A CN201580054416 A CN 201580054416A CN 106796175 B CN106796175 B CN 106796175B
Authority
CN
China
Prior art keywords
sample
sample well
energy
instrument
excitation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201580054416.4A
Other languages
English (en)
Chinese (zh)
Other versions
CN106796175A (zh
Inventor
乔纳森·M·罗思伯格
阿里·卡比里
杰森·W·斯克勒
布雷特·J·格亚凡思
杰瑞米·拉基
杰勒德·施密德
保罗·E·格伦
罗伦斯·C·威斯特
本杰明·西普里亚尼
基思·G·法夫
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quantum Si Inc
Original Assignee
Quantum Si Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum Si Inc filed Critical Quantum Si Inc
Publication of CN106796175A publication Critical patent/CN106796175A/zh
Application granted granted Critical
Publication of CN106796175B publication Critical patent/CN106796175B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/04—Batch operation; multisample devices
    • G01N2201/0446—Multicell plate, sequential
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Optics & Photonics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Diffracting Gratings Or Hologram Optical Elements (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Optical Measuring Cells (AREA)
CN201580054416.4A 2014-08-08 2015-08-07 用于探测、检测和分析分子的光学系统和检测芯片 Active CN106796175B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462035242P 2014-08-08 2014-08-08
US62/035,242 2014-08-08
PCT/US2015/044378 WO2016023010A1 (en) 2014-08-08 2015-08-07 Optical system and assay chip for probing, detecting, and analyzing molecules

Publications (2)

Publication Number Publication Date
CN106796175A CN106796175A (zh) 2017-05-31
CN106796175B true CN106796175B (zh) 2021-01-05

Family

ID=53887235

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580054416.4A Active CN106796175B (zh) 2014-08-08 2015-08-07 用于探测、检测和分析分子的光学系统和检测芯片

Country Status (10)

Country Link
US (5) US9921157B2 (enExample)
EP (2) EP3194933B1 (enExample)
JP (1) JP6812341B2 (enExample)
KR (2) KR102472061B1 (enExample)
CN (1) CN106796175B (enExample)
AU (1) AU2015300778B2 (enExample)
BR (1) BR112017002489B1 (enExample)
CA (1) CA2957543A1 (enExample)
MX (1) MX383892B (enExample)
WO (1) WO2016023010A1 (enExample)

Families Citing this family (157)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US8933526B2 (en) * 2009-07-15 2015-01-13 First Solar, Inc. Nanostructured functional coatings and devices
US9599508B2 (en) 2012-05-18 2017-03-21 Rebellion Photonics, Inc. Divided-aperture infra-red spectral imaging system
WO2013173541A1 (en) 2012-05-18 2013-11-21 Rebellion Photonics, Inc. Divided-aperture infra-red spectral imaging system for chemical detection
CA2881823C (en) 2012-08-20 2019-06-11 Illumina, Inc. Method and system for fluorescence lifetime based sequencing
EP4480582A1 (en) 2013-01-18 2024-12-25 Biomeme, Inc. Analytic device
CN204138341U (zh) * 2013-04-18 2015-02-04 崔波 硅衬底上的硅柱阵列
JP5808021B2 (ja) * 2013-07-16 2015-11-10 国立大学法人名古屋大学 電子親和力の低下処理装置に用いられる活性化容器及びキット、該キットを含む電子親和力の低下処理装置、フォトカソード電子ビーム源、並びに、フォトカソード電子ビーム源を含む電子銃、自由電子レーザー加速器、透過型電子顕微鏡、走査型電子顕微鏡、電子線ホログラフィー顕微鏡、電子線描画装置、電子線回折装置及び電子線検査装置
GB201319438D0 (en) * 2013-11-04 2013-12-18 Univ Lancaster Waveguide
CA2870419C (en) 2013-11-12 2023-05-16 Rebellion Photonics, Inc. Divided-aperture infra-red spectral imaging system
MX392471B (es) 2013-11-17 2025-03-24 Quantum Si Inc Sistema optico y chip de ensayo para sondear, detectar y analizar moleculas
US9756263B2 (en) 2014-05-01 2017-09-05 Rebellion Photonics, Inc. Mobile gas and chemical imaging camera
US11290662B2 (en) 2014-05-01 2022-03-29 Rebellion Photonics, Inc. Mobile gas and chemical imaging camera
US10458905B2 (en) 2014-07-07 2019-10-29 Rebellion Photonics, Inc. Gas leak emission quantification with a gas cloud imager
EP3194935B1 (en) 2014-08-08 2018-10-31 Quantum-si Incorporated Integrated device for temporal binning of received photons
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
BR112017002501B1 (pt) 2014-08-08 2022-06-07 Quantum-Si Incorporated Dispositivos integrados, sistemas, método de detecção de presença de molécula em amostra, método de formação de dispositivo integrado e método de análise de espécime
KR102472061B1 (ko) * 2014-08-08 2022-11-29 퀀텀-에스아이 인코포레이티드 분자들을 프로빙, 검출 및 분석하기 위한 광학계 및 검정 칩
JP6179682B2 (ja) * 2015-01-22 2017-08-16 株式会社村田製作所 空隙配置構造体およびその製造方法
US10267956B2 (en) 2015-04-14 2019-04-23 California Institute Of Technology Multi-wavelength optical dielectric metasurfaces
JP6878306B2 (ja) * 2015-05-20 2021-05-26 クアンタム−エスアイ インコーポレイテッドＱｕａｎｔｕｍ−Ｓｉ Ｉｎｃｏｒｐｏｒａｔｅｄ パルスレーザ及び生物分析システム
CA3241702A1 (en) 2015-05-29 2016-12-08 Rebellion Photonics, Inc. Hydrogen sulfide imaging system
KR102027264B1 (ko) * 2015-08-18 2019-10-01 가부시키가이샤 후지킨 농도 측정 장치
US10881336B2 (en) * 2015-08-21 2021-01-05 California Institute Of Technology Planar diffractive device with matching diffraction spectrum
US9927477B1 (en) * 2015-09-09 2018-03-27 Cpg Technologies, Llc Object identification system and method
US10097281B1 (en) 2015-11-18 2018-10-09 Hypres, Inc. System and method for cryogenic optoelectronic data link
US10352856B2 (en) * 2015-12-14 2019-07-16 Massachusetts Institute Of Technology Apparatus and methods for spectroscopy and broadband light emission using two-dimensional plasmon fields
US10670782B2 (en) 2016-01-22 2020-06-02 California Institute Of Technology Dispersionless and dispersion-controlled optical dielectric metasurfaces
KR20180111999A (ko) 2016-02-17 2018-10-11 테서렉트 헬스, 인코포레이티드 수명 촬상 및 검출 응용을 위한 센서 및 디바이스
FI20165148A7 (fi) * 2016-02-25 2017-08-26 Arcdia Int Oy Ltd Kaksoisfotoniviritteistä fluoresenssia hyödyntävä bioaffiniteettimääritysmenetelmä
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
EP3446157B1 (en) * 2016-04-22 2023-05-17 Illumina, Inc. Photonic stucture-based devices and compositions for use in luminescent imaging of multiple sites within a pixel, and methods of using the same
KR102627237B1 (ko) * 2016-06-01 2024-01-23 퀀텀-에스아이 인코포레이티드 분자들을 검출 및 분석하기 위한 통합 디바이스
AU2017274412B2 (en) * 2016-06-01 2022-07-21 Quantum-Si Incorporated Pulse caller and base caller
JP6771147B2 (ja) * 2016-06-22 2020-10-21 パナソニックＩｐマネジメント株式会社 浴光システム及び携帯端末
US11124827B2 (en) * 2016-06-23 2021-09-21 Roche Sequencing Solutions, Inc. Period-to-period analysis of AC signals from nanopore sequencing
US11373278B2 (en) * 2016-09-30 2022-06-28 University Of Utah Research Foundation Lensless imaging device
EP3305170B1 (en) * 2016-10-04 2020-12-02 ams AG Fluorescence lifetime sensor module and method of determining a fluorescence lifetime using a sensor module
WO2018075957A1 (en) 2016-10-21 2018-04-26 Rebellion Photonics, Inc. Gas imaging system
EP3529573A4 (en) 2016-10-21 2020-05-20 Rebellion Photonics, Inc. Mobile gas and chemical imaging camera
US10890528B2 (en) * 2016-10-27 2021-01-12 Sharp Kabushiki Kaisha Fluorescent testing system, molecular testing method, and fluorescent testing method
WO2018112170A1 (en) 2016-12-16 2018-06-21 Quantum-Si Incorporated Compact beam shaping and steering assembly
CA3046680A1 (en) 2016-12-19 2018-06-28 Quantum-Si Incorporated Loading molecules into sample wells for analysis
CN110168732B (zh) 2016-12-22 2024-03-08 宽腾矽公司 具有直接合并像素的整合式光电侦测器
WO2018132487A1 (en) * 2017-01-10 2018-07-19 Photoswitch Biosciences, Inc. Systems and methods for detection
CN207396531U (zh) 2017-01-31 2018-05-22 杭州探真纳米科技有限公司 一种悬臂末端纳米探针
WO2018156795A1 (en) 2017-02-22 2018-08-30 Rebellion Photonics, Inc. Systems and methods for monitoring remote installations
EP4414690A3 (en) * 2017-03-20 2024-11-20 MGI Tech Co., Ltd. Biosensors for biological or chemical analysis and methods of manufacturing the same
US10379288B2 (en) * 2017-04-07 2019-08-13 Lijie Qiao Method and device having a saturable absorber for filtering
US10488651B2 (en) 2017-04-10 2019-11-26 California Institute Of Technology Tunable elastic dielectric metasurface lenses
WO2018204810A1 (en) 2017-05-05 2018-11-08 Quantum-Si Incorporated Substrates having modified surface reactivity and antifouling properties in biological reactions
JP7072847B2 (ja) * 2017-05-12 2022-05-23 公立大学法人大阪 被検出物質の検出キットおよびそれを備えた検出システム、ならびに、被検出物質の検出キットの製造方法
US20180364223A1 (en) * 2017-06-16 2018-12-20 Krista Michelle Fretes Portable single-molecule bio-sensing device
AU2018282868B2 (en) * 2017-06-16 2023-10-19 Duke University Resonator networks for improved label detection, computation, analyte sensing, and tunable random number generation
CN107463727B (zh) * 2017-06-27 2021-01-19 浙江大学 基于向量式有限元和fpga的混合试验方法
US10222350B2 (en) * 2017-07-12 2019-03-05 International Business Machines Corporation High sensitivity force gauge with parallel dipole line trap system
KR102786367B1 (ko) * 2017-07-24 2025-03-26 퀀텀-에스아이 인코포레이티드 광학 거부 광자 구조체들
CN111133293A (zh) * 2017-07-24 2020-05-08 宽腾矽公司 手持式大规模并行生物光电仪器
EP3438649B1 (en) * 2017-07-31 2020-03-11 Vestel Elektronik Sanayi ve Ticaret A.S. Identification tag and method of identifying an object
CN107332106B (zh) * 2017-08-01 2019-10-15 复旦大学 全硅分布式反馈激光器
EP3669018A4 (en) 2017-08-18 2021-05-26 Nautilus Biotechnology, Inc. Binding reagent selection methods
EP3682024A4 (en) 2017-09-15 2021-05-12 Biomeme, Inc. METHODS AND SYSTEMS FOR AUTOMATIC SAMPLE PROCESSING
US10811772B2 (en) * 2017-09-18 2020-10-20 The United States Of America As Represented By The Secretary Of The Army Concentric, co-located and interleaved dual band antenna array
CN111527397B (zh) 2017-11-09 2023-11-17 逆转光子公司 红外ir成像系统及其光学窗口遮蔽检测方法
JP7524061B2 (ja) 2017-12-15 2024-07-29 バイオミーム インコーポレイテッド サンプルを分析するためのポータブル装置及び方法
CN109932316A (zh) * 2017-12-18 2019-06-25 长光华大基因测序设备（长春）有限公司 基因测序光学装置
NL2020615B1 (en) 2017-12-26 2019-07-02 Illumina Inc Image sensor structure
CN111512155B (zh) 2017-12-28 2022-07-05 豪夫迈·罗氏有限公司 测量和去除来自交流信号驱动的纳米孔dna测序系统的随机信号中的噪声
EP3737503A2 (en) * 2018-01-08 2020-11-18 Quantum-Si Incorporated System and methods for electrokinetic loading of sub-micron-scale reaction chambers
AU2019205496B2 (en) 2018-01-08 2021-08-19 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
MX2020007904A (es) 2018-01-26 2020-09-07 Quantum Si Inc Llamado de pulso y base habilitado por maquina de aprendizaje para dispositivos de secuenciacion.
NL2021357A (en) * 2018-01-31 2018-08-16 Asml Netherlands Bv Two-dimensional diffraction grating
GB201802597D0 (en) * 2018-02-16 2018-04-04 Vision Rt Ltd A calibration object for calibrating a patient monitoring system
CA3108295A1 (en) 2018-06-22 2019-12-26 Quantum-Si Incorporated Integrated photodetector with charge storage bin of varied detection time
CN109085224B (zh) * 2018-08-27 2023-11-03 浙江大学 用于细胞表面区域atp检测的敏感微电极
EP3844486A1 (en) * 2018-08-29 2021-07-07 Quantum-Si Incorporated System and methods for detecting lifetime using photon counting photodetectors
CN110910888B (zh) * 2018-09-17 2022-06-14 中国移动通信集团设计院有限公司 语音识别装置及方法
US10796191B2 (en) * 2018-09-26 2020-10-06 Stmicroelectronics Sa Device and method for processing a histogram of arrival times in an optical sensor
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
EP3884048A4 (en) 2018-11-20 2022-08-17 Nautilus Biotechnology, Inc. DESIGN AND SELECTION OF AFFINITY REAGENTS
CN109603942B (zh) * 2019-02-15 2021-12-24 京东方科技集团股份有限公司 微流控装置和微流控方法
CN113544493A (zh) * 2019-03-05 2021-10-22 宽腾矽公司 用于集成装置的光学吸收滤光器
JP7483745B2 (ja) * 2019-03-21 2024-05-15 バイオミーム インコーポレイテッド 多機能性分析デバイス
EP3963091A4 (en) 2019-04-29 2023-07-19 Nautilus Biotechnology, Inc. METHODS AND SYSTEMS FOR ON-CHIP INTEGRATED SINGLE MOLECULE DETECTION
US12313627B2 (en) 2019-05-01 2025-05-27 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US11828709B2 (en) 2019-05-17 2023-11-28 GeneSense Technology Inc. Analytical system for molecule detection and sensing
US11011366B2 (en) * 2019-06-06 2021-05-18 Kla Corporation Broadband ultraviolet illumination sources
US11009611B2 (en) 2019-06-18 2021-05-18 Eagle Technology, Llc Radiation detection system with surface plasmon resonance detection and related methods
CN110229747A (zh) * 2019-06-18 2019-09-13 京东方科技集团股份有限公司 基因测序芯片、设备及制备方法
US11085878B2 (en) 2019-06-18 2021-08-10 Eagle Technology, Llc Radiation detection system with surface plasmon resonance detection and related methods
EP3980755A1 (en) 2019-06-19 2022-04-13 Quantum-Si Incorporated Optical nanostructure rejecter for an integrated device and related methods
KR20220025853A (ko) 2019-06-28 2022-03-03 퀀텀-에스아이 인코포레이티드 광학적 및 전기적 2차 경로 제거
MY209672A (en) 2019-06-28 2025-07-30 Illumina Inc Flowcells with linear waveguides
CN114514420A (zh) 2019-08-08 2022-05-17 宽腾矽公司 集成光学装置中的增加的发射收集效率
EP3779408A1 (de) * 2019-08-15 2021-02-17 optek-Danulat GmbH Messvorrichtung und verfahren zur bestimmung einer stoffkonzentration
AU2020364058A1 (en) 2019-10-11 2022-05-26 Quantum-Si Incorporated Surface modification in the vapor phase
FR3102848B1 (fr) * 2019-10-30 2021-11-19 Commissariat Energie Atomique Dispositif d'excitation et de détection de photoluminescence
KR20220091534A (ko) 2019-10-31 2022-06-30 퀀텀-에스아이 인코포레이티드 강화된 드레인을 갖는 픽셀
CN111123428B (zh) * 2019-12-24 2022-03-08 中国科学院苏州生物医学工程技术研究所 一种零模波导孔孔壁的修饰方法及零模波导孔结构
CN111123429B (zh) * 2019-12-24 2022-03-08 中国科学院苏州生物医学工程技术研究所 涂覆有锚定物质的零模波导孔的制备方法及波导孔结构
EP4088319A1 (en) 2020-01-14 2022-11-16 Quantum-si Incorporated Sensor for lifetime plus spectral characterization
CN115210969A (zh) 2020-01-14 2022-10-18 宽腾矽公司 调幅激光器
US11060138B1 (en) * 2020-01-17 2021-07-13 Element Biosciences, Inc. Nucleic acid sequencing systems
US20210285881A1 (en) * 2020-02-18 2021-09-16 Pacific Biosciences Of California, Inc. Highly multiplexed nucleic acid sequencing systems
US11719639B2 (en) 2020-03-02 2023-08-08 Quantum-Si Incorporated Integrated sensor for multi-dimensional signal analysis
CN113686940A (zh) * 2020-03-20 2021-11-23 上海芯像生物科技有限公司 用于分子检测和感测的高通量分析系统
EP4133324B1 (en) * 2020-04-06 2025-10-08 Rarecyte, Inc. Optical trains for imaging systems and spectral edge detection
EP3901612B1 (en) * 2020-04-23 2024-06-12 Sensirion AG Integrated particulate matter sensor with cavity
CN111551531B (zh) * 2020-05-19 2023-04-18 北京金诺美科技股份有限公司 一种荧光激发系统及实时荧光定量pcr仪
WO2021236720A1 (en) * 2020-05-20 2021-11-25 Ysi, Inc. Extended solid angle turbidity sensor
US11555994B2 (en) * 2020-05-22 2023-01-17 The Texas A&M University System Wide-field deep UV Raman microscope
CA3182266A1 (en) * 2020-06-11 2021-12-16 Vadim Lobanov Methods and systems for computational decoding of biological, chemical, and physical entities
CN116323965A (zh) * 2020-07-23 2023-06-23 生命技术公司 使用染料的用于生物分析的组合物、系统和方法
EP4213989A4 (en) 2020-09-18 2025-01-15 Biomeme, Inc. Portable devices and methods for analyzing samples
KR20230093003A (ko) 2020-10-22 2023-06-26 퀀텀-에스아이 인코포레이티드 순차적으로 결합된 전하 저장소를 갖는 집적 회로 및 연관된 기술들
WO2022087438A1 (en) * 2020-10-23 2022-04-28 Quantum-Si Incorporated Systems and methods for sample process scaling
US12181460B2 (en) 2020-11-30 2024-12-31 Gentroma, Inc. Semiconductor nanosensing device with multilayer graphene sheet for sequencing or sensing nucleic acids
JP2023554368A (ja) 2020-12-15 2023-12-27 クアンタム－エスアイ インコーポレイテッド 単一分子速度論による分子バーコード分析
AU2022209365A1 (en) 2021-01-21 2023-07-20 Nautilus Subsidiary, Inc. Systems and methods for biomolecule preparation
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
CN112986206B (zh) * 2021-05-20 2021-07-30 北京百奥纳芯生物科技有限公司 一种检测基因芯片杂交结果的方法
CN113640327B (zh) * 2021-06-03 2023-07-25 中国工程物理研究院材料研究所 一种大曲率微小件表面多层金属薄膜的无损检测方法
CN113433104A (zh) * 2021-07-05 2021-09-24 夏晓艺 基于等离子体增强的单分子检测模块、防伪标签及应用
IL310281A (en) 2021-07-21 2024-03-01 Element Biosciences Inc Optical systems for nucleic acid sequencing and methods thereof
CA3227872A1 (en) 2021-09-09 2023-03-16 James Henry JOLY Characterization and localization of protein modifications
EP4405683A1 (en) 2021-09-22 2024-07-31 Nautilus Subsidiary, Inc. Methods and systems for determining polypeptide interactions
EP4420167A4 (en) 2021-10-22 2025-05-07 Illumina Inc. Semiconductor light sensing
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
US11662526B1 (en) * 2021-12-09 2023-05-30 Visera Technologies Company Ltd. Optical structure
KR102578658B1 (ko) 2021-12-10 2023-09-14 한국세라믹기술원 미소 충전 샘플링에 의한 ｐｅｂ 유도 에너지 분석 방법
AU2023241893A1 (en) 2022-03-29 2024-09-26 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
CN114879243B (zh) * 2022-04-13 2023-04-25 中国科学院近代物理研究所 一种基于光子计数的束流光谱检测装置及方法
TWI830229B (zh) * 2022-05-11 2024-01-21 國立成功大學 微型檢測機及其檢測模組
US20250376716A1 (en) 2022-06-21 2025-12-11 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
US12146833B2 (en) * 2022-10-14 2024-11-19 Toyota Motor Engineering & Manufacturing North America, Inc. Chiral sensing with harmonic generation from a metasurface fabricated on a SOI wafer
EP4375644A1 (en) * 2022-11-22 2024-05-29 Gnothis Holding AG Fingerprint analysis of single molecule events
EP4382614A1 (en) * 2022-12-06 2024-06-12 Gnothis Holding AG High yield support
WO2024124073A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. A method comprising performing on a single-analyte array at least 50 cycles of a process
US20240201182A1 (en) 2022-12-15 2024-06-20 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
WO2024206122A1 (en) 2023-03-24 2024-10-03 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces
CN116106263B (zh) * 2023-04-07 2023-06-16 成都甄识科技有限公司 一种高灵敏度高品质因数的超表面局域等离激元传感器
WO2024229409A2 (en) * 2023-05-03 2024-11-07 Tomphyzx.Llc Optical probe for measuring photon density
WO2024238038A1 (en) * 2023-05-12 2024-11-21 Redshift Bioanalytics, Inc. Matched optical filter
WO2024243237A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
US20240411078A1 (en) * 2023-06-08 2024-12-12 Ii-Vi Delaware, Inc. Polarizer, diffraction grating and meta surface fabrication via ion implantation
US12444022B2 (en) * 2023-06-30 2025-10-14 Fei Company Focus stacking applications for sample preparation
CN117042425B (zh) * 2023-07-03 2024-08-09 成都飞机工业（集团）有限责任公司 一种吸波型频率选择表面电磁屏蔽结构
US20250066841A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
WO2025137166A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
CN117608082B (zh) * 2024-01-23 2024-05-03 江苏金视传奇科技有限公司 压缩成像系统、压缩成像方法
WO2025160569A1 (en) * 2024-01-26 2025-07-31 Pumpkinseed Technologies, Inc. Methods and systems for nucleic acid sequence determination
WO2025199236A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
WO2025212338A1 (en) 2024-04-01 2025-10-09 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014031157A1 (en) * 2012-08-20 2014-02-27 Illumina, Inc. Method and system for fluorescence lifetime based sequencing

Family Cites Families (164)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
JPH05281041A (ja) * 1992-03-31 1993-10-29 Omron Corp 分光器
CA2155186A1 (en) 1993-02-01 1994-08-18 Kevin M. Ulmer Methods and apparatus for dna sequencing
US6456326B2 (en) 1994-01-28 2002-09-24 California Institute Of Technology Single chip camera device having double sampling operation
US5471515A (en) 1994-01-28 1995-11-28 California Institute Of Technology Active pixel sensor with intra-pixel charge transfer
PL317379A1 (en) 1994-05-27 1997-04-01 Ciba Geigy Ag Supercritically excited luminescence detecting process
JPH0815590A (ja) * 1994-06-29 1996-01-19 Olympus Optical Co Ltd 光学要素位置決め装置
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5814565A (en) 1995-02-23 1998-09-29 University Of Utah Research Foundation Integrated optic waveguide immunosensor
US6261797B1 (en) 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
US5990506A (en) 1996-03-20 1999-11-23 California Institute Of Technology Active pixel sensors with substantially planarized color filtering elements
US5929986A (en) * 1996-08-26 1999-07-27 Kaiser Optical Systems, Inc. Synchronous spectral line imaging methods and apparatus
US6198869B1 (en) 1996-11-18 2001-03-06 Novartis Ag Measuring device and its use
ATE273381T1 (de) 1997-02-12 2004-08-15 Eugene Y Chan Verfahren zur analyse von polymeren
EP2208789B1 (en) 1997-03-12 2015-07-22 Applied Biosystems, LLC DNA polymerases having improved labeled nucleotide incorporation properties
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US6825921B1 (en) 1999-11-10 2004-11-30 Molecular Devices Corporation Multi-mode light detection system
EP0921196A1 (en) 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
DE19810879A1 (de) 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
WO1999049082A2 (en) 1998-03-23 1999-09-30 Invitrogen Corporation Modified nucleotides and methods useful for nucleic acid sequencing
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
WO1999057321A1 (en) 1998-05-01 1999-11-11 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
GB9810350D0 (en) 1998-05-14 1998-07-15 Ciba Geigy Ag Organic compounds
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6716394B2 (en) 1998-08-11 2004-04-06 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6280939B1 (en) 1998-09-01 2001-08-28 Veeco Instruments, Inc. Method and apparatus for DNA sequencing using a local sensitive force detector
US6232075B1 (en) 1998-12-14 2001-05-15 Li-Cor, Inc. Heterogeneous assay for pyrophosphate detection
AU3174600A (en) 1999-03-10 2000-09-28 Asm Scientific, Inc. A method for direct nucleic acid sequencing
EP1681356B1 (en) 1999-05-19 2011-10-19 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6137117A (en) 1999-06-21 2000-10-24 The United States Of America As Represented By The Secretary Of The Navy Integrating multi-waveguide sensor
US6399335B1 (en) 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
US6331438B1 (en) 1999-11-24 2001-12-18 Iowa State University Research Foundation, Inc. Optical sensors and multisensor arrays containing thin film electroluminescent devices
US7175811B2 (en) 2000-04-28 2007-02-13 Edgelight Biosciences Micro-array evanescent wave fluorescence detection device
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
JP2004516810A (ja) 2000-06-07 2004-06-10 リ−コール インコーポレーティッド 電荷スイッチヌクレオチド
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
US7158224B2 (en) 2000-06-25 2007-01-02 Affymetrix, Inc. Optically active substrates
FR2813121A1 (fr) 2000-08-21 2002-02-22 Claude Weisbuch Dispositif perfectionne de support d'elements chromophores
EP1320596A4 (en) 2000-08-30 2005-01-12 Univ Rochester REVERSE TRANSCRIPTION REACTION METHOD USING REVERSE TRANSCRIPTASE CODED BY RETROTRANSPOSON NON-LTR
WO2002023167A1 (en) 2000-09-11 2002-03-21 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Fluidics system
AU2002258997A1 (en) 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
US7052839B2 (en) 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7041812B2 (en) 2001-08-29 2006-05-09 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US7727722B2 (en) 2001-08-29 2010-06-01 General Electric Company Ligation amplification
US7244566B2 (en) 2001-08-29 2007-07-17 Ge Healthcare Bio-Sciences Corp. Analyte detection
US7223541B2 (en) 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
US7033762B2 (en) 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7256019B2 (en) 2001-08-29 2007-08-14 Ge Healthcare Bio-Sciences Corp. Terminal phosphate blocked nucleoside polyphosphates
JP2005508630A (ja) 2001-09-14 2005-04-07 インヴィトロジェン コーポレーション Ｄｎａポリメラーゼとその変異体
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7179654B2 (en) 2002-03-18 2007-02-20 Agilent Technologies, Inc. Biochemical assay with programmable array detection
US6924887B2 (en) 2002-03-27 2005-08-02 Sarnoff Corporation Method and apparatus for generating charge from a light pulse
US6768122B2 (en) * 2002-06-04 2004-07-27 National Taiwan University Multiphoton excitation microscope for biochip fluorescence assay
US7244961B2 (en) * 2002-08-02 2007-07-17 Silicon Valley Scientific Integrated system with modular microfluidic components
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
JP4896708B2 (ja) 2003-02-05 2012-03-14 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 新規リンカー類を有する末端リン酸標識ヌクレオチド類
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
JP4268461B2 (ja) * 2003-06-24 2009-05-27 浜松ホトニクス株式会社 時間分解測定装置
US20050074784A1 (en) * 2003-10-07 2005-04-07 Tuan Vo-Dinh Integrated biochip with continuous sampling and processing (CSP) system
WO2005073407A1 (en) * 2003-10-07 2005-08-11 Ut-Battelle, Llc Advanced integrated circuit biochip
US7002688B2 (en) * 2003-10-16 2006-02-21 Pria Diagnostics, Inc. Multilens optical assembly for a diagnostic device
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005069737A2 (en) 2004-01-27 2005-08-04 Ramot At Tel Aviv University Ltd. Method and system for detecting analytes
DE602005020421D1 (de) 2004-02-19 2010-05-20 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US8128871B2 (en) * 2005-04-22 2012-03-06 Alverix, Inc. Lateral flow assay systems and methods
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
WO2006044078A2 (en) 2004-09-17 2006-04-27 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US8685711B2 (en) * 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
CA2588122A1 (en) * 2004-11-16 2006-05-26 Helicos Biosciences Corporation Tirf single molecule analysis and method of sequencing nucleic acids
US7345764B2 (en) 2005-02-07 2008-03-18 Vladimir Bulovic Apparatus and method for a slim format spectrometer
US20060223067A1 (en) 2005-03-31 2006-10-05 Paolo Vatta Mutant DNA polymerases and methods of use
US7738086B2 (en) 2005-05-09 2010-06-15 The Trustees Of Columbia University In The City Of New York Active CMOS biosensor chip for fluorescent-based detection
EP1907584A2 (en) 2005-06-28 2008-04-09 Agencourt Personal Genomics Corporation Methods of producing and sequencing modified polynucleotides
WO2007045998A2 (en) 2005-07-01 2007-04-26 Dako Denmark A/S New nucleic acid base pairs
US7426322B2 (en) 2005-07-20 2008-09-16 Searete Llc. Plasmon photocatalysis
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
DE602006016984D1 (de) 2005-12-12 2010-10-28 Us Gov Health & Human Serv Sonde zum sequenzieren von nukleinsäure und verwendungsverfahren
AU2006331512B2 (en) 2005-12-22 2012-02-23 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
CA2633524A1 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
US7995202B2 (en) * 2006-02-13 2011-08-09 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7715001B2 (en) 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7692783B2 (en) 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US20070188736A1 (en) * 2006-02-16 2007-08-16 Fouquet Julie E Obtaining measurement and baseline signals for evaluating assay test strips
JP4782593B2 (ja) * 2006-03-13 2011-09-28 株式会社日立製作所 光検出装置
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
US20080050747A1 (en) 2006-03-30 2008-02-28 Pacific Biosciences Of California, Inc. Articles having localized molecules disposed thereon and methods of producing and using same
DE102006030541B4 (de) 2006-06-23 2010-05-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Optische Anordnung
US8207509B2 (en) 2006-09-01 2012-06-26 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
CA2662521C (en) 2006-09-01 2016-08-09 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
JP5395323B2 (ja) * 2006-09-29 2014-01-22 ブレインビジョン株式会社 固体撮像素子
US20100096561A1 (en) * 2006-10-05 2010-04-22 Koninklijke Philips Electronics N.V. Methods and systems for detection with front irradiation
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
FR2908888B1 (fr) 2006-11-21 2012-08-03 Centre Nat Rech Scient Dispositif pour la detection exaltee de l'emission d'une particule cible
EP1925929A1 (en) * 2006-11-27 2008-05-28 Koninklijke Philips Electronics N.V. Multivariate detection of molecules in bioassay
US8821799B2 (en) * 2007-01-26 2014-09-02 Palo Alto Research Center Incorporated Method and system implementing spatially modulated excitation or emission for particle characterization with enhanced sensitivity
ATE509265T1 (de) 2007-03-22 2011-05-15 Univ Strasbourg Etablissement Public A Caractere Scient Culturel Et Professionel Gerät zur sortierung und konzentration elektromagnetischer energie und vorrichtung mit mindestens einem derartigen gerät
EP2134871B1 (en) 2007-03-30 2012-03-07 Pacific Biosciences of California, Inc. Systems and methods for enhancing fluorescent signals
US7873085B2 (en) 2007-10-23 2011-01-18 Andrei Babushkin Method and device for controlling optical output of laser diode
WO2009082706A1 (en) 2007-12-21 2009-07-02 The Trustees Of Columbia University In The City Of New York Active cmos sensor array for electrochemical biomolecular detection
WO2009091847A2 (en) 2008-01-14 2009-07-23 Life Technologies Corporation Compositions, methods and systems for single molecule sequencing
AU2009212165A1 (en) 2008-02-07 2009-08-13 Pacific Biosciences Of California, Inc. CIS reactive oxygen quenchers integrated into linkers
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
WO2009102470A2 (en) 2008-02-12 2009-08-20 Pacific Biosciences Of California, Inc. Compositions and methods for use in analytical reactions
US7968702B2 (en) 2008-03-13 2011-06-28 Pacific Biosciences Of California, Inc. Labeled reactants and their uses
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
CN102084001B (zh) 2008-03-28 2015-03-18 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序的组合物和方法
US8236499B2 (en) 2008-03-28 2012-08-07 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
US8143030B2 (en) 2008-09-24 2012-03-27 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2009145818A1 (en) 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc Single molecule loading methods and compositions
AU2009251883B2 (en) 2008-03-31 2014-09-11 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
WO2009145828A2 (en) 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc. Two slow-step polymerase enzyme systems and methods
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
US8420366B2 (en) 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US9127259B2 (en) 2008-03-31 2015-09-08 Pacific Biosciences Of California, Inc. Enzymes resistant to photodamage
CA2735979A1 (en) 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc. Engineering polymerases and reaction conditions for modified incorporation properties
CA2974241C (en) 2008-09-16 2020-01-07 Pacific Biosciences Of California, Inc. Substrates and optical systems and methods of use thereof
US8921046B2 (en) 2008-09-19 2014-12-30 Pacific Biosciences Of California, Inc. Nucleic acid sequence analysis
US8481264B2 (en) 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8247216B2 (en) 2008-09-30 2012-08-21 Pacific Biosciences Of California, Inc. Ultra-high multiplex analytical systems and methods
EP2182523B1 (en) 2008-10-31 2013-01-09 CSEM Centre Suisse d'Electronique et de Microtechnique SA -Recherche et Développement Charge sampling device and method based on a MOS-transmission line
WO2010057185A1 (en) 2008-11-17 2010-05-20 Pacific Biosciences Of California, Inc. Phospholink nucleotides for sequencing applications
JP2010161629A (ja) * 2009-01-08 2010-07-22 Fujifilm Corp 固体撮像素子及びその駆動方法並びに撮像装置
CA2750874A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Hybridization linkers
US20100261185A1 (en) 2009-03-27 2010-10-14 Life Technologies Corporation Labeled enzyme compositions, methods and systems
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
US8501406B1 (en) 2009-07-14 2013-08-06 Pacific Biosciences Of California, Inc. Selectively functionalized arrays
WO2011040971A2 (en) 2009-09-30 2011-04-07 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8278728B2 (en) 2009-10-17 2012-10-02 Florida Institute Of Technology Array of concentric CMOS photodiodes for detection and de-multiplexing of spatially modulated optical channels
US9579402B2 (en) 2009-12-04 2017-02-28 Biotium, Inc. Heterocycle-substituted xanthene dyes
JP6017107B2 (ja) 2009-12-28 2016-10-26 ソニー株式会社 イメージセンサ及びその製造方法、並びにセンサデバイス
US20110236983A1 (en) 2009-12-29 2011-09-29 Joseph Beechem Single molecule detection and sequencing using fluorescence lifetime imaging
EP2537053B1 (en) 2010-02-19 2016-11-02 Pacific Biosciences of California, Inc. An analytical device comprising an optode array chip
US8994946B2 (en) 2010-02-19 2015-03-31 Pacific Biosciences Of California, Inc. Integrated analytical system and method
JP5021054B2 (ja) * 2010-05-25 2012-09-05 キヤノン株式会社 屈折率分布計測方法および屈折率分布計測装置
US8865078B2 (en) * 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8865077B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
JP2012047719A (ja) * 2010-07-28 2012-03-08 Furukawa Electric Advanced Engineering Co Ltd マルチ光測定装置、マルチ光測定方法、マルチ光スイッチ
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
US8773665B1 (en) * 2011-04-01 2014-07-08 Emcore Corporation Compact fiber optic gyroscope
WO2013024637A1 (ja) * 2011-08-12 2013-02-21 オリンパス株式会社 蛍光粒子の検出方法
JP6010034B2 (ja) * 2011-08-30 2016-10-19 オリンパス株式会社 標的粒子の検出方法
KR102130856B1 (ko) * 2011-09-30 2020-07-08 라이프 테크놀로지스 코포레이션 생물학적 분석을 위한 광학 시스템 및 방법
US8778849B2 (en) 2011-10-28 2014-07-15 Illumina, Inc. Microarray fabrication system and method
US9606060B2 (en) 2012-01-13 2017-03-28 California Institute Of Technology Filterless time-domain detection of one or more fluorophores
US8906660B2 (en) 2012-02-01 2014-12-09 Pacific Biosciences Of California, Inc. Recombinant polymerases with increased phototolerance
EP3222627B1 (en) 2012-02-15 2019-08-07 Pacific Biosciences of California, Inc. Polymerase enzyme substrates with protein shield
WO2013171197A1 (en) 2012-05-15 2013-11-21 Ait Austrian Institute Of Technology Gmbh Compact plasmon-enhanced fluorescence biosensor
US9372308B1 (en) 2012-06-17 2016-06-21 Pacific Biosciences Of California, Inc. Arrays of integrated analytical devices and methods for production
WO2014065329A1 (ja) * 2012-10-25 2014-05-01 浜松ホトニクス株式会社 細胞観察装置及び細胞観察方法
EP3734255B1 (en) 2012-12-18 2022-10-19 Pacific Biosciences Of California, Inc. An optical analytical device
MX392471B (es) * 2013-11-17 2025-03-24 Quantum Si Inc Sistema optico y chip de ensayo para sondear, detectar y analizar moleculas
US9765395B2 (en) 2014-04-28 2017-09-19 Nanomedical Diagnostics, Inc. System and method for DNA sequencing and blood chemistry analysis
BR112017002501B1 (pt) 2014-08-08 2022-06-07 Quantum-Si Incorporated Dispositivos integrados, sistemas, método de detecção de presença de molécula em amostra, método de formação de dispositivo integrado e método de análise de espécime
KR102472061B1 (ko) * 2014-08-08 2022-11-29 퀀텀-에스아이 인코포레이티드 분자들을 프로빙, 검출 및 분석하기 위한 광학계 및 검정 칩
EP3194935B1 (en) 2014-08-08 2018-10-31 Quantum-si Incorporated Integrated device for temporal binning of received photons
US9666748B2 (en) 2015-01-14 2017-05-30 International Business Machines Corporation Integrated on chip detector and zero waveguide module structure for use in DNA sequencing
EP4220256A1 (en) 2015-03-16 2023-08-02 Pacific Biosciences of California, Inc. Analytical system comprising integrated devices and systems for free-space optical coupling

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014031157A1 (en) * 2012-08-20 2014-02-27 Illumina, Inc. Method and system for fluorescence lifetime based sequencing

Also Published As

Publication number Publication date
KR20170041848A (ko) 2017-04-17
US11879841B2 (en) 2024-01-23
US11175227B2 (en) 2021-11-16
MX2017001807A (es) 2018-02-08
EP3194933A1 (en) 2017-07-26
US10371634B2 (en) 2019-08-06
US20220120685A1 (en) 2022-04-21
EP4424846A2 (en) 2024-09-04
MX383892B (es) 2025-03-14
BR112017002489A2 (pt) 2017-12-05
AU2015300778B2 (en) 2021-02-25
AU2015300778A1 (en) 2017-03-16
KR102472061B1 (ko) 2022-11-29
BR112017002489B1 (pt) 2023-02-14
US20190383739A1 (en) 2019-12-19
US20180231465A1 (en) 2018-08-16
JP2017531168A (ja) 2017-10-19
US20240248034A1 (en) 2024-07-25
JP6812341B2 (ja) 2021-01-13
CA2957543A1 (en) 2016-02-11
US20160041095A1 (en) 2016-02-11
CN106796175A (zh) 2017-05-31
WO2016023010A1 (en) 2016-02-11
KR102738114B1 (ko) 2024-12-06
US9921157B2 (en) 2018-03-20
KR20220165282A (ko) 2022-12-14
EP4424846A3 (en) 2024-11-20
EP3194933B1 (en) 2024-05-01

Similar Documents

Publication Publication Date Title
CN106796175B (zh) 用于探测、检测和分析分子的光学系统和检测芯片
CN106796176B (zh) 用于对分子进行探测、检测和分析的带外部光源的集成装置
CN111235246B (zh) 用于探测、检测和分析分子的光学系统和测定芯片
HK40055175B (en) Integrated device with external light source for probing, detecting, and analyzing molecules
HK40055175A (en) Integrated device with external light source for probing, detecting, and analyzing molecules
HK1241967A1 (en) Instrument for time resolved reading of fluorescence based assay chip
HK1228964A1 (en) Optical system and assay chip for probing, detecting and analyzing molecules
HK1228964B (en) Optical system and assay chip for probing, detecting and analyzing molecules

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant