ATE518010T1 - Aktive oberflächengekoppelte polymerasen - Google Patents

Aktive oberflächengekoppelte polymerasen

Info

Publication number
ATE518010T1
ATE518010T1 AT06848008T AT06848008T ATE518010T1 AT E518010 T1 ATE518010 T1 AT E518010T1 AT 06848008 T AT06848008 T AT 06848008T AT 06848008 T AT06848008 T AT 06848008T AT E518010 T1 ATE518010 T1 AT E518010T1
Authority
AT
Austria
Prior art keywords
active surface
surface coupled
polymerases
coupled polymerases
making
Prior art date
Application number
AT06848008T
Other languages
English (en)
Inventor
David Hanzel
Jonas Korlach
Paul Peluso
Geoffrey Otto
Thang Pham
David Rank
Stephen Turner
Original Assignee
Pacific Biosciences California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pacific Biosciences California filed Critical Pacific Biosciences California
Application granted granted Critical
Publication of ATE518010T1 publication Critical patent/ATE518010T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT06848008T 2005-12-22 2006-12-21 Aktive oberflächengekoppelte polymerasen ATE518010T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US75351505P 2005-12-22 2005-12-22
PCT/US2006/048973 WO2007075987A2 (en) 2005-12-22 2006-12-21 Active surface coupled polymerases

Publications (1)

Publication Number Publication Date
ATE518010T1 true ATE518010T1 (de) 2011-08-15

Family

ID=38218654

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06848008T ATE518010T1 (de) 2005-12-22 2006-12-21 Aktive oberflächengekoppelte polymerasen

Country Status (6)

Country Link
US (2) US8936926B2 (de)
EP (1) EP1963530B1 (de)
AT (1) ATE518010T1 (de)
AU (1) AU2006331512B2 (de)
CA (1) CA2633476C (de)
WO (1) WO2007075987A2 (de)

Families Citing this family (159)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
AU2006330947B2 (en) 2005-12-22 2012-04-12 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
CN101495656B (zh) 2006-06-07 2017-02-08 纽约哥伦比亚大学理事会 采用带修饰的核苷酸通过纳米通道进行dna序列测定
CA2700838A1 (en) 2007-09-28 2009-04-09 Pacific Biosciences Of California, Inc. Error-free amplification of dna for clonal sequencing
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
US7811810B2 (en) 2007-10-25 2010-10-12 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
US7767441B2 (en) 2007-10-25 2010-08-03 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
EP2263087B1 (de) 2008-03-13 2017-08-09 Pacific Biosciences of California, Inc. Markierte reaktanden und ihre verwendung
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
EP3045542B1 (de) 2008-03-28 2016-11-16 Pacific Biosciences of California, Inc. Verfahren zur nukleinsäuresequenzierung
EP2274446B1 (de) 2008-03-31 2015-09-09 Pacific Biosciences of California, Inc. Systeme und verfahren für polymeraseenzymreaktionen in zwei langsamen schritten
US8420366B2 (en) 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US9637380B2 (en) 2008-03-31 2017-05-02 Pacific Biosciences Of California, Inc. Nanoscale apertures having islands of functionality
CA2720046C (en) 2008-03-31 2018-07-24 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
EP2286217B1 (de) 2008-03-31 2015-02-18 Pacific Biosciences of California, Inc. Verfahren und zusammensetzung zur ladung von einzelmolekülen
US9127259B2 (en) 2008-03-31 2015-09-08 Pacific Biosciences Of California, Inc. Enzymes resistant to photodamage
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
EP2307540B1 (de) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzymporenstrukturen
JP2011527191A (ja) 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
EP2326733A2 (de) 2008-09-05 2011-06-01 Pacific Biosciences of California, Inc. Manipulierte polymerasen und reaktionsbedingungen für modifzierte einbringungseigenschaften
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
WO2010086622A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
BRPI1007215A2 (pt) 2009-01-30 2017-08-29 Oxford Nanopore Tech Ltd Método de acoplamento covalente de duas ou mais porções, primeira e segunda porções, primeiro porção acoplada a uma segunda porção, par de primeiro e segundo ligantes, e, uso de um par de ligantes.
US9778188B2 (en) 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
WO2011031313A2 (en) 2009-09-11 2011-03-17 Pacific Biosciences Of California, Inc. Zero-mode waveguides with non-reflecting walls
US8367159B2 (en) 2009-09-11 2013-02-05 Pacific Biosciences Of California, Inc. Methods for producing ZMWs with islands of functionality
US8911972B2 (en) 2009-12-16 2014-12-16 Pacific Biosciences Of California, Inc. Sequencing methods using enzyme conformation
US20110192723A1 (en) * 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US9410891B2 (en) 2010-02-19 2016-08-09 Pacific Biosciences Of California, Inc. Optics collection and detection system and method
US9482615B2 (en) * 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8865077B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8986930B2 (en) 2010-07-12 2015-03-24 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
US8889373B2 (en) 2010-08-12 2014-11-18 Eastman Chemical Company Enzyme catalyst immobilized on porous fluoropolymer support
WO2012027623A2 (en) 2010-08-25 2012-03-01 Pacific Biosciences Of California, Inc. Cyanine dyes
US8936911B2 (en) 2010-09-22 2015-01-20 Pacific Biosciences Of California, Inc. Purified extended polymerase/template complex for sequencing
ES2641871T3 (es) 2010-12-17 2017-11-14 The Trustees Of Columbia University In The City Of New York Secuenciación de ADN mediante síntesis usando nucleótidos modificados y detección con nanoporos
US9121059B2 (en) 2010-12-22 2015-09-01 Genia Technologies, Inc. Nanopore-based single molecule characterization
EP2658988B1 (de) 2010-12-27 2018-01-24 Ibis Biosciences, Inc. Verfahren und zusammensetzungen für die herstellung von nukleinsäureproben
US9581563B2 (en) 2011-01-24 2017-02-28 Genia Technologies, Inc. System for communicating information from an array of sensors
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
BR112014001699A2 (pt) 2011-07-25 2017-06-13 Oxford Nanopore Tech Ltd método para sequenciar de um polinucleotídeo alvo de filamento duplo, kit, métodos para preparar um polinucleotídeo alvo de filamento duplo para sequenciamento e para sequenciar um polinucleotídeo alvo de filamento duplo, e, aparelho
WO2013022778A1 (en) 2011-08-05 2013-02-14 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
US9347900B2 (en) 2011-10-14 2016-05-24 Pacific Biosciences Of California, Inc. Real-time redox sequencing
WO2013101743A2 (en) 2011-12-30 2013-07-04 Abbott Molecular, Inc. Microorganism nucelic acid purification from host samples
EP2802666B1 (de) 2012-01-13 2018-09-19 Data2Bio Genotypisierung durch eine sequenzierung der nächsten generation
US9528107B2 (en) 2012-01-31 2016-12-27 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
US8906660B2 (en) 2012-02-01 2014-12-09 Pacific Biosciences Of California, Inc. Recombinant polymerases with increased phototolerance
US9062091B2 (en) 2012-02-15 2015-06-23 Pacific Biosciences Of California, Inc. Polymerase enzyme substrates with protein shield
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
EP2836604B1 (de) 2012-04-09 2021-09-15 The Trustees of Columbia University in the City of New York Verfahren zur herstellung von nanoporen und verwendungen davon
CA2869546C (en) 2012-04-10 2020-07-21 Oxford Nanopore Technologies Limited Mutant lysenin pores
ES2833524T3 (es) 2012-05-02 2021-06-15 Ibis Biosciences Inc Secuenciación de ADN
WO2013166305A1 (en) 2012-05-02 2013-11-07 Ibis Biosciences, Inc. Dna sequencing
ES2683707T3 (es) 2012-05-02 2018-09-27 Ibis Biosciences, Inc. Secuenciación de ADN
US9315864B2 (en) 2012-05-18 2016-04-19 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes with sulfonic acid substituents
EP2850086B1 (de) 2012-05-18 2023-07-05 Pacific Biosciences Of California, Inc. Heteroarylcyaninfarbstoffe
GB2557509A (en) 2012-06-08 2018-06-20 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
WO2013188841A1 (en) 2012-06-15 2013-12-19 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
EP2875154B1 (de) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB-Verfahren zur Charakterisierung einer Nukleinsäure
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
CA2887665A1 (en) 2012-10-16 2014-04-24 Abbott Molecular Inc. Methods and apparatus to sequence a nucleic acid
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
US9290800B2 (en) 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
US10732183B2 (en) 2013-03-15 2020-08-04 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
EP2994544B1 (de) 2013-05-06 2019-10-02 Pacific Biosciences Of California, Inc. Elektronische echtzeit-sequenzierung
US10544449B2 (en) 2013-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
CN105308073B (zh) 2013-06-14 2019-08-13 加利福尼亚太平洋生物科学股份有限公司 双生物素化标签
ES2764096T3 (es) 2013-08-19 2020-06-02 Abbott Molecular Inc Bibliotecas de secuenciación de próxima generación
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
JP6461943B2 (ja) 2013-10-23 2019-01-30 ジェニア・テクノロジーズ・インコーポレイテッド ナノポアを備えた高速分子検知
US9567630B2 (en) 2013-10-23 2017-02-14 Genia Technologies, Inc. Methods for forming lipid bilayers on biochips
US9983135B2 (en) 2013-11-17 2018-05-29 Quantum-Si Incorporated Active-source-pixel, integrated device for rapid analysis of biological and chemical specimens
US10768181B2 (en) 2013-12-17 2020-09-08 The Brigham And Women's Hospital, Inc. Detection of an antibody against a pathogen
WO2015110813A1 (en) 2014-01-22 2015-07-30 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
CN106715453B (zh) 2014-03-24 2021-04-30 哥伦比亚大学董事会 用于生产带标签的核苷酸的化学方法
EP3137490B1 (de) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutante poren
EP3161157B1 (de) 2014-06-24 2024-03-27 Bio-Rad Laboratories, Inc. Digitales pcr-barcoding
EP3161490B1 (de) 2014-06-27 2019-10-02 Abbott Laboratories Zusammensetzungen und verfahren zur detektion des menschlichen pegivirus 2 (hpgv-2)
BR112017002485B1 (pt) 2014-08-08 2020-12-01 Quantum-Si Incorporated circuito integrado e método de fotodetecção
CA2957546A1 (en) 2014-08-08 2016-02-11 Quantum-Si Incorporated Integrated device with external light source for probing, detecting, and analyzing molecules
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
JP6812341B2 (ja) 2014-08-08 2021-01-13 クアンタム−エスアイ インコーポレイテッドＱｕａｎｔｕｍ−Ｓｉ Ｉｎｃｏｒｐｏｒａｔｅｄ 分子の探索、検出及び解析のための光学システム及びアッセイチップ
US10435685B2 (en) 2014-08-19 2019-10-08 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
EP3183367B1 (de) 2014-08-19 2019-06-26 Pacific Biosciences Of California, Inc. Zusammensetzungen und verfahren zur anreicherung von nukleinsäuren
WO2016034591A2 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant pores
WO2016044391A1 (en) 2014-09-17 2016-03-24 Ibis Biosciences, Inc. Sequencing by synthesis using pulse read optics
EP3204511B1 (de) 2014-10-07 2021-07-28 Oxford Nanopore Technologies Limited Mutante poren
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
WO2016154345A1 (en) 2015-03-24 2016-09-29 Pacific Biosciences Of California, Inc. Methods and compositions for single molecule composition loading
WO2016166232A1 (en) 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
ES2897823T3 (es) 2015-04-30 2022-03-02 Curevac Ag Poli(N)polimerasa inmovilizada
WO2017024017A1 (en) 2015-08-06 2017-02-09 Pacific Biosciences Of California, Inc. Systems and methods for selectively addressing sparsely arranged electronic measurement devices
WO2017027518A1 (en) 2015-08-10 2017-02-16 Stratos Genomics, Inc. Single molecule nucleic acid sequencing with molecular sensor complexes
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
US10814299B2 (en) 2015-11-18 2020-10-27 Pacific Biosciences Of California, Inc. Loading nucleic acids onto substrates
US10731211B2 (en) 2015-11-18 2020-08-04 Pacific Biosciences Of California, Inc. Methods and compositions for loading of polymerase complexes
US10781483B2 (en) 2015-11-20 2020-09-22 Pacific Biosciences Of California, Inc. Labeled nucleotide analogs, reaction mixtures, and methods and systems for sequencing
CN117024498A (zh) 2015-11-20 2023-11-10 加利福尼亚太平洋生物科学股份有限公司 受保护的染料标记的试剂
JP7237586B2 (ja) 2015-12-08 2023-03-13 カトリック ユニヴェルシテット ルーヴェン カーユー ルーヴェン リサーチ アンド ディベロップメント 修飾ナノポア、それを含む組成物およびその使用
US10730030B2 (en) 2016-01-08 2020-08-04 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
TWI734748B (zh) 2016-02-17 2021-08-01 美商太斯萊特健康股份有限公司 用於生命期成像及偵測應用之感測器及裝置
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
CA3020203A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN106021749B (zh) * 2016-05-25 2019-03-05 上海交通大学 反推二维格栅的稠度优化实现方法
US10544457B2 (en) 2016-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Methods and compositions for enriching compositions for polymerase enzyme complexes
US10711300B2 (en) 2016-07-22 2020-07-14 Pacific Biosciences Of California, Inc. Methods and compositions for delivery of molecules and complexes to reaction sites
US11543417B2 (en) 2016-08-29 2023-01-03 Oslo Universitetssykehus Hf ChIP-seq assays
ES2870639T3 (es) 2016-10-24 2021-10-27 Geneinfosec Inc Ocultación de información presente en los ácidos nucleicos
US11021738B2 (en) 2016-12-19 2021-06-01 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented DNA
CN118099177A (zh) 2016-12-22 2024-05-28 宽腾矽公司 具有直接合并像素的整合式光电侦测器
CN110573616B (zh) * 2017-04-18 2023-09-05 深圳华大智造科技股份有限公司 Phi29 DNA聚合酶及其编码基因与应用
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
MX2019013111A (es) 2017-05-05 2019-12-16 Quantum Si Inc Sustratos que tienen reactividad de superficie modificada y propiedades antiincrustantes en reacciones biologicas.
EP3625240A4 (de) 2017-05-18 2021-01-27 Ultima Genomics, Inc. Sequenzierung mit nichtnatürlichen nukleotiden
EP3642363B1 (de) 2017-06-20 2025-12-24 Bio-Rad Laboratories, Inc. Mda mit bead-oligonukleotid
CN110914290A (zh) 2017-06-30 2020-03-24 弗拉芒区生物技术研究所 新颖蛋白孔
TW201915473A (zh) 2017-07-24 2019-04-16 美商寬騰矽公司 手持，大量平行之生物光電裝置
US11162138B2 (en) 2017-10-30 2021-11-02 Pacific Biosciences Of California, Inc. Multi-amplitude modular labeled compounds
CN118186055A (zh) 2017-11-02 2024-06-14 生物辐射实验室股份有限公司 基于转座酶的基因组分析
CN111788485A (zh) 2017-12-22 2020-10-16 加利福尼亚太平洋生物科学股份有限公司 用于固定化的修饰型生物素结合蛋白
WO2019152395A1 (en) 2018-01-31 2019-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
CA3133334A1 (en) 2018-03-13 2019-09-19 Inanc ORTAC Methods for single molecule sequencing
US11512002B2 (en) 2018-04-18 2022-11-29 University Of Virginia Patent Foundation Silica materials and methods of making thereof
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
US11391626B2 (en) 2018-06-22 2022-07-19 Quantum-Si Incorporated Integrated photodetector with charge storage bin of varied detection time
WO2020006421A1 (en) 2018-06-29 2020-01-02 Pacific Biosciences Of California, Inc. Methods and compositions for delivery of molecules and complexes to reaction sites
EP4249651B1 (de) 2018-08-20 2025-01-29 Bio-Rad Laboratories, Inc. Generierung von nukleotidsequenzen durch barcode-kügelchen-co-lokalisierung in partitionen
EP3674702A1 (de) 2018-12-27 2020-07-01 Imec VZW Verfahren zur sequenzierung eines polynukleotids mittels eines biofet
US11773422B2 (en) 2019-08-16 2023-10-03 Microsoft Technology Licensing, Llc Regulation of polymerase using cofactor oxidation states
US11795450B2 (en) 2019-09-06 2023-10-24 Microsoft Technology Licensing, Llc Array-based enzymatic oligonucleotide synthesis
EP4028570A1 (de) 2019-10-11 2022-07-20 Quantum-Si Incorporated Oberflächenmodifizierung in der dampfphase
CN110713519A (zh) * 2019-11-02 2020-01-21 深圳市安帝宝科技有限公司 一种11-脱氢血栓烷b2抗原及其制备方法
US12006535B2 (en) 2019-11-19 2024-06-11 Sarmal, Inc. Methods and devices for detecting SARS-COV-2
JP2023502472A (ja) 2019-11-19 2023-01-24 サーマル，インコーポレイテッド Ｓａｒｓ－ｃｏｖ－２を検出する方法およびデバイス
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
US11702683B2 (en) 2020-05-28 2023-07-18 Microsoft Technology Licensing, Llc De novo polynucleotide synthesis with substrate-bound polymerase
WO2022084748A1 (en) * 2020-10-23 2022-04-28 Spindle Biotech, Inc. Compositions and methods for rna synthesis
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
WO2025244989A1 (en) 2024-05-21 2025-11-27 Pacific Biosciences Of California, Inc. Selectively functionalized nanoscale wells
WO2026019720A1 (en) 2024-07-15 2026-01-22 Pacific Biosciences Of California, Inc. Improved loading of molecules and complexes to reaction sites

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5496934A (en) 1993-04-14 1996-03-05 Yissum Research Development Company Of The Hebrew University Of Jerusalem Nucleic acids encoding a cellulose binding domain
US5614365A (en) 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US6383483B1 (en) 1995-01-06 2002-05-07 Désiré José Collen Staphylokinase derivatives with cysteine substitutions
US5998580A (en) * 1995-10-13 1999-12-07 Fay; Frederick F. Photosensitive caged macromolecules
US6261797B1 (en) * 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
US5972603A (en) 1996-02-09 1999-10-26 President And Fellows Of Harvard College DNA polymerase with modified processivity
US5736373A (en) 1996-09-23 1998-04-07 Becton, Dickinson And Company Thermostable DNA polymerase from Bacillus pallidus
AU9312598A (en) * 1997-09-10 1999-03-29 Allegheny University Of The Health Sciences Inhibitors of collagen assembly
CA2243985C (en) * 1997-09-11 2004-08-10 F. Hoffmann-La Roche Ag Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
US6077940A (en) * 1997-12-24 2000-06-20 Genentech, Inc. Free solution ligand interaction molecular separation method
DE19810879A1 (de) 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
DK1159453T3 (da) * 1999-03-10 2008-10-06 Asm Scient Inc Fremgangsmåde til direkte nukleinsyresekventering
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US20030050231A1 (en) * 2000-01-31 2003-03-13 Rosen Craig A. Nucleic acids, proteins, and antibodies
US20030140369A1 (en) * 2000-02-29 2003-07-24 Pioneer Hi-Bred International, Inc. Novel defense induced multi-drug resistance genes and uses thereof
ATE452989T1 (de) * 2000-03-27 2010-01-15 Univ Jefferson Zusammensetzungen und methoden zur identifizierung von krebszellen
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US20030036181A1 (en) * 2000-06-30 2003-02-20 Okkels Jens Sigurd Peptide extended glycosylated polypeptides
DE10034804A1 (de) * 2000-07-18 2002-01-31 Bayer Ag Verwendung von VLCFAE zum Identifizieren von herbizid wirksamen Verbindungen
WO2002029027A1 (en) 2000-10-04 2002-04-11 Ahram Biosystems Inc. Immobilized dna polymerase
US7238505B2 (en) 2000-10-04 2007-07-03 Ahram Biosystems Inc. Immobilized DNA polymerase
WO2002086088A2 (en) 2001-04-24 2002-10-31 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
US7205106B1 (en) 2001-07-20 2007-04-17 Roche Molecular Systems, Inc. Association of polymorphisms in IL4-related genes with autoimmune disease
US7560254B2 (en) 2001-08-29 2009-07-14 Ge Healthcare Bio-Sciences Corp. Allele specific primer extension
ATE458067T1 (de) 2001-08-29 2010-03-15 Ge Healthcare Bio Sciences Markierte nukleosidpolyphosphate
US20050187718A1 (en) * 2001-09-21 2005-08-25 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from Streptococcus pneumoniae
US7745116B2 (en) * 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
AU2006330947B2 (en) 2005-12-22 2012-04-12 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation

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US8323939B2 (en) 2012-12-04
US20080199932A1 (en) 2008-08-21
CA2633476A1 (en) 2007-07-05
WO2007075987A2 (en) 2007-07-05
US8936926B2 (en) 2015-01-20
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AU2006331512A1 (en) 2007-07-05
EP1963530A4 (de) 2009-08-05
EP1963530B1 (de) 2011-07-27

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