CN103055003B - Pure Chinese medicinal composition for treating fin rot of fish and prawns and preparation method of pure Chinese medicinal composition - Google Patents
Pure Chinese medicinal composition for treating fin rot of fish and prawns and preparation method of pure Chinese medicinal composition Download PDFInfo
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Abstract
The invention relates to a pure Chinese medicinal composition for treating the fin rot of fish and prawns. The pure Chinese medicinal composition is prepared by mixing the following components in part by weight: 15 to 35 parts of red-knees herb, 15 to 35 parts of folium artemisiae argyi, 15 to 35 parts of sweet wormwood herb, 15 to 35 parts of propolis, 15 to 35 parts of tripterygium wilfordii, 15 to 35 parts of houttuynia cordata, 20 to 40 parts of astragalus, 20 to 40 parts of dried orange peel, 20 to 40 parts of bupleurum, 20 to 40 parts of szechuan lovage rhizome, 15 to 35 parts of Vietnamese sophora root and 20 to 40 parts of licorice root. The invention has the advantages that the pure Chinese medicinal composition is rational in formula, easy to prepare, free of toxic or side effects, medicament residues and medicament resistance and high in superfine grinding degree and bioavailability, can treat both symptoms and root causes and has remarkable curative effects, and clinical results prove that the pure Chinese medicinal composition has a special effect of treating the fin rot of the fish and the prawns.
Description
Technical field
The present invention relates to pure Chinese medicine composition for the treatment of fish and shrimp gill rot and preparation method thereof, belong to animal field of medicaments, particularly a kind of pure Chinese medicine composition that is specifically designed to treatment fish and shrimp gill rot and preparation method thereof.
Background technology
Fish and shrimp gill rot is a kind of common disease rapid, that the course of disease is grown that infects in the fall.Gill rot is mainly divided into three kinds, is respectively parasitic gill rot, bacterial gill rot and fungoid gill rot.Parasitic gill rot is the various gill diseases that parasite causes, colonizes on the gill of fish and breeding, and the gill portion that stimulates fish, secretes a large amount of mucus, and gill filament enlargement, variable color, have a strong impact on the breathing of fish; Bacterial gill rot disease is invaded and is caused by myxobacter, and show as the gill filament and rot, with mud, the hyperemia of gill cover inner surface, central epidermis is often etched into a duck eye that circle is transparent, is commonly called as blank; Fungoid gill rot is by the mould disease causing of the gill, shows as disease fish gill portion and is pale asphyxia, sometimes has point-like hyperemia or bleeding.
This disease very easily occurs, and spreads rapidly, wayward, often makes fishy fulminant ground dead, causes serious economic loss to fish and shrimp aquaculture.
This disease almost spreads all over all fish and shrimp cultivation country of the world, is widely distributed.Primary disease western medicine mainly uses antiparasitic and antibiosis anti-inflammatory drug, though receive certain effect, because its toxic and side effects by force, easily produces the problem such as drug resistance and drug residue, clinical practice is very limited or bans use of.At present, treat the most frequently used Chinese medicine of this disease because its prescription is unreasonable or just simple pulverizing, particle diameter is excessive, and drug release is slower, causes its bioavailability lower, and clinical effectiveness is not fine.
Therefore research and develop a kind of pure Chinese prescription for the treatment of fish and shrimp gill rot, micronizing, bioavailability are high, and the specific medicament for the treatment of both the principal and secondary aspects of a disease is one of this field scientific research technical staff new problem anxious to be resolved.
Summary of the invention
The object of the invention is to overcome above-mentioned weak point, provide a kind of reasonable recipe, preparation simple, micronizing, bioavailability are high, treating both the principal and secondary aspects of a disease, pure Chinese medicine composition of effective treatment fish and shrimp gill rot of successful and preparation method thereof.
Implementation of the present invention is as follows for achieving the above object: a kind of pure Chinese medicine composition for the treatment of fish and shrimp gill rot, by following component by weight mixed preparing form: Herba polygoni hydropiperis 15-35 part, Folium Artemisiae Argyi 15-35 part, Herba Artemisiae Annuae 15-35 part, propolis 15-35 part, Radix Tripterygii Wilfordii 15-35 part, Herba Houttuyniae 15-35 part, Radix Astragali 20-40 part, Pericarpium Citri Reticulatae 20-40 part, Radix Bupleuri 20-40 part, Rhizoma Chuanxiong 20-40 part, Radix Sophorae Tonkinensis 15-35 part, Radix Glycyrrhizae 20-40 part.
Described various component optimum ratio amount is: 25 parts of Herba polygoni hydropiperis, 25 parts of Folium Artemisiae Argyis, 25 parts of Herba Artemisiae Annuaes, 25 parts of propolis, 25 parts of Radix Tripterygii Wilfordiis, 25 parts of Herba Houttuyniae, 30 parts of the Radixs Astragali, 30 parts of Pericarpium Citri Reticulataes, 30 parts of Radix Bupleuri, 30 parts of Rhizoma Chuanxiongs, 25 parts of Radix Sophorae Tonkinensiss, 30 parts, Radix Glycyrrhizae.
Treat a preparation method for the pure Chinese medicine composition of fish and shrimp gill rot, carry out as follows:
(1) the each composition in prescription is crossed to 700 mesh sieves after micronizing respectively;
(2) take by weight Herba polygoni hydropiperis 15-35 part, Folium Artemisiae Argyi 15-35 part, Herba Artemisiae Annuae 15-35 part, propolis 15-35 part, Radix Tripterygii Wilfordii 15-35 part, Herba Houttuyniae 15-35 part, mix 5-10 minute to even;
(3) take by weight Radix Astragali 20-40 part, Pericarpium Citri Reticulatae 20-40 part, Radix Bupleuri 20-40 part, Rhizoma Chuanxiong 20-40 part, Radix Sophorae Tonkinensis 15-35 part, Radix Glycyrrhizae 20-40 part, mix 5-10 minute to even;
(4) merge the compositions that above-mentioned (2), (3) step make, continue to mix 10-15 minute to evenly and get final product.
The medicinal property of each component is as follows:
Herba polygoni hydropiperis: pungent, warm, return lung, liver, large intestine channel, there is the function of removing toxic substances, damp eliminating, dissipating blood stasis, hemostasis; Cure mainly dysentery, rheumatic arthralgia, carbuncle furuncle, eczema, traumatic hemorrhage etc.
Propolis: bitter, acrid, cold, return spleen, stomach warp, the function that there is anti-inflammation, antalgesic-antipruritic, promotion tissue regeneration, improve immunity, antiallergic is oxidized, stops blooding, protects gastrointestinal mucosa etc.
Rhizoma Chuanxiong: pungent, warm, return gallbladder, liver, pericardium channel, there is the function of blood-activating and qi-promoting, wind-expelling pain-stopping; Cure mainly traumatic injury, stagnation of QI-blood, puerperal blood stasis, rheumatic arthralgia etc.
Herba Artemisiae Annuae: bitter, acrid, cold, return liver, gallbladder meridian, there is the function of heat-clearing and toxic substances removing, protozoacide; Cure mainly coccidiosis, jaundice due to damp-heat etc.
Radix Tripterygii Wilfordii: pungent, bitter, cool, return liver, kidney channel, there is the function of expelling wind and removing dampness, removing obstruction in the collateral to relieve pain, reducing swelling and alleviating pain, removing toxic substances parasite killing; Cure mainly that skin is itched, rheumatic arthralgia, the yellow furunculosis of skin ulcer etc.
Herba Houttuyniae: pungent, be slightly cold, return lung meridian, there is the functions such as heat-clearing and toxic substances removing, detumescence and apocenosis, inducing diuresis for treating stranguria syndrome, cure mainly dysentery, intestinal Huang, lung abscess, acute mastitis, stranguria with turbid discharge etc.
The Radix Astragali: sweet, tepor, return spleen, lung meridian, there is the function of tonifying Qi and lifting yang, strengthening superficial resistance to stop perspiration, inducing diuresis to remove edema, expelling pus and toxin by strengthening QI, expelling pus and promoting granulation; Curing mainly asthenia of pulmonosplenic qi, sinking of QI of middle-JIAO, exterior deficiency spontaneous perspiration, deficiency of vital energy edema, carbuncle sore difficultly bursts, bursts for a long time and do not hold back etc.
Pericarpium Citri Reticulatae: bitter, pungent, warm, return lung, spleen channel, there is the function of regulating qi-flowing for strengthening spleen, drying dampness to eliminate phlegm; Cure mainly poor appetite, stomachache, tripe is swollen, have loose bowels, productive cough due to phlegm-damp etc.
Radix Bupleuri: pungent, bitter, be slightly cold, return liver, gallbladder, lung meridian, there is the function of delivering with inner, elevate a turnable ladder yang-energy, dispersing the stagnated live-QI to relieve the stagnation of QI; Cure mainly cold, fever, alternate attack of chill and fever, insufficiency of the spleen chronic diarrhea, proctoptosis etc.
Folium Artemisiae Argyi: acrid, bitter, warm, return liver,kidney,spleen warp, there is the function of dispersing cold for relieving pain, warming the meridian for stopping bleeding; Cure mainly anemofrigid-damp arthralgia, tripe abdomen cold type of pain etc.
Radix Sophorae Tonkinensis: cold, bitter, attach to the lung and stomach meridians, there is the function of heat-clearing and toxic substances removing, detumescence sore-throat relieving, expelling phlegm for arresting cough; Cure mainly laryngopharynx swelling and pain, lung-heat cough with asthma, the yellow furunculosis of skin ulcer etc.
Radix Glycyrrhizae: sweet, flat, return lung, the heart, spleen channel, there is invigorating the spleen and replenishing QI, expelling phlegm for arresting cough and middle emergency, removing toxic substances, coordinating the actions of various ingredients in a prescription, cushion toxicity, strong function; Cure mainly weakness of the spleen and stomach, cough with asthma, poisoning etc.
All herbal medicines, according to the theoretical compatible combination of Chinese veterinarian, for paathogenic factor and the clinical symptoms of fish and shrimp gill rot, are brought into play the effect for the treatment of both the principal and secondary aspects of a disease above.
The invention has the beneficial effects as follows: reasonable recipe, preparation is simple, and substep mixes more even; Pure Chinese medicine composition, treating both the principal and secondary aspects of a disease, cost is low, draws materials wide, and micronizing, bioavailability are high, curative effect highly significant.The present invention, according to Chinese veterinarian's theory, optimizes this 12 herbal medicine, and they is formed according to optimum ratio, and clinical practice proves that treatment fish and shrimp gill rot is had to specially good effect.
Detailed description of the invention
Below in conjunction with preferred embodiment, to according to detailed description of the invention provided by the invention, details are as follows:
Embodiment 1
A kind of pure Chinese medicine composition for the treatment of fish and shrimp gill rot and preparation method thereof: carry out as follows: (every part by 10g)
(1) the each composition in prescription is crossed to 700 mesh sieves after micronizing respectively;
(2) take by weight 150 parts of Herba polygoni hydropiperis, 150 parts of Folium Artemisiae Argyis, 150 parts of Herba Artemisiae Annuaes, 150 parts of propolis, 150 parts of Radix Tripterygii Wilfordiis, 150 parts of Herba Houttuyniae, mix 5-10 minute to even;
(3) take by weight 200 parts of the Radixs Astragali, 200 parts of Pericarpium Citri Reticulataes, 200 parts of Radix Bupleuri, 200 parts of Rhizoma Chuanxiongs, 150 parts of Radix Sophorae Tonkinensiss, 200 parts, Radix Glycyrrhizae, mix 5-10 minute to even;
(4) merge the compositions that above-mentioned (2), (3) step make, continue to mix 10-15 minute to evenly and get final product.
Embodiment 2
A kind of pure Chinese medicine composition for the treatment of fish and shrimp gill rot and preparation method thereof: carry out as follows: (every part by 10g)
(1) the each composition in prescription is crossed to 700 mesh sieves after micronizing respectively;
(2) take by weight 250 parts of Herba polygoni hydropiperis, 250 parts of Folium Artemisiae Argyis, 250 parts of Herba Artemisiae Annuaes, 250 parts of propolis, 250 parts of Radix Tripterygii Wilfordiis, 250 parts of Herba Houttuyniae, mix 5-10 minute to even;
(3) take by weight 300 parts of the Radixs Astragali, 300 parts of Pericarpium Citri Reticulataes, 300 parts of Radix Bupleuri, 300 parts of Rhizoma Chuanxiongs, 250 parts of Radix Sophorae Tonkinensiss, 300 parts, Radix Glycyrrhizae, mix 5-10 minute to even;
(4) merge the compositions that above-mentioned (2), (3) step make, continue to mix 10-15 minute to evenly and get final product.
Embodiment 3
A kind of pure Chinese medicine composition for the treatment of fish and shrimp gill rot and preparation method thereof: carry out as follows: (every part by 10g)
(1) the each composition in prescription is crossed to 700 mesh sieves after micronizing respectively;
(2) take by weight 350 parts of Herba polygoni hydropiperis, 350 parts of Folium Artemisiae Argyis, 350 parts of Herba Artemisiae Annuaes, 350 parts of propolis, 350 parts of Radix Tripterygii Wilfordiis, 350 parts of Herba Houttuyniae, mix 5-10 minute to even;
(3) take by weight 400 parts of the Radixs Astragali, 400 parts of Pericarpium Citri Reticulataes, 400 parts of Radix Bupleuri, 400 parts of Rhizoma Chuanxiongs, 350 parts of Radix Sophorae Tonkinensiss, 400 parts, Radix Glycyrrhizae, mix 5-10 minute to even;
(4) merge the compositions that above-mentioned (2), (3) step make, continue to mix 10-15 minute to evenly and get final product.
Clinical trial:
Clinical experiment animal selects 150 ages in days, body weight at 1.0kg-1.5kg, and Laboratory Diagnosed is the ill fish of Ctenopharyngodon idellus gill rot.Ill Ctenopharyngodon idellus isolation is raised separately, the complete feed of feeding, and be divided at random 3 groups, and every group of 30 tails, 3 groups of feeding environments are identical, and experiment is carried out 5 days altogether; Blank group is left intact, and medicine matched group uses treatment with " fish and shrimp rotten cheek is loose " by specification; Present composition group can be for oral administration by product of the present invention (the prepared compositions of the embodiment of the present invention 1), admixes bait feeding in 2% ratio, every day 2-3 time, is used in conjunction 3-5 days; After oral, reach the object for the treatment of disease by gastrointestinal absorption.Computational methods: mortality rate=dead animal number/experimental animal number × 100%, cure rate=healing number of animals/experimental animal number × 100%.Result (testing the 5th day) when following table is experiment end.
Table 1 is the field applicable cases contrast of the present invention and other medicines treatment Ctenopharyngodon idellus gill rot:
Numbering | Group | Mortality rate % | Cure rate % | When end, there is symptom rate % |
I | Blank group | 43.3 | 36.7 | 20.0 |
II | The fish and shrimp rotten cheek group of faling apart | 10.0 | 76.7 | 13.3 |
III | Present composition group | 3.3 | 86.7 | 10.0 |
This clinical effectiveness statistical analysis, the loose group of present composition treatment group and fish and shrimp rotten cheek extremely significantly (P < 0.01) of difference compared with aspect cure rate.
Clinical experiment animal selects 300 ages in days, body weight at 0.04kg-0.06kg, and Laboratory Diagnosed is the ill shrimp of Macrobrachium rosenbergii gill rot.Ill shrimp isolation is raised separately, the complete feed of feeding, and be divided at random 3 groups, and every group of 100 tails, 3 groups of feeding environments are identical, and experiment is carried out 5 days altogether; Blank group is left intact, and medicine matched group uses treatment with " fish and shrimp rotten cheek is loose " by specification; Present composition group can be for oral administration by product of the present invention (the prepared compositions of the embodiment of the present invention 1), admixes bait feeding in 2.5% ratio, every day 2-3 time, is used in conjunction 3-5 days; After oral, reach the object for the treatment of disease by gastrointestinal absorption.Computational methods: mortality rate=dead animal number/experimental animal number × 100%, cure rate=healing number of animals/experimental animal number × 100%.Result (testing the 5th day) when following table is experiment end.
Table 2 is field applicable cases contrasts of the present invention and other medicines treatment shrimp gill rot:
Numbering | Group | Mortality rate % | Cure rate % | When end, there is symptom rate % |
I | Blank group | 53 | 27 | 20 |
II | The fish and shrimp rotten cheek group of faling apart | 13 | 77 | 10 |
III | Present composition group | 8 | 85 | 7 |
This clinical effectiveness statistical analysis, the loose group of present composition treatment group and fish and shrimp rotten cheek significant difference (P < 0.05) compared with aspect cure rate.
Safety testing:
Choose 80 age in days healthy grass carp 30 tails, 5 times according to the made compositions recommended dose of the embodiment of the present invention 1 take orally 10 days, during medication, there is not any bad or Deviant Behavior performance in healthy grass carp, the drinking-water of searching for food is normal, and body weight increases that to compare difference not remarkable with blank group (being not administration of health group).
Choose healthy Macrobrachium rosenbergii 30 tails of 100 ages in days, 5 times according to the made compositions recommended dose of the embodiment of the present invention 1 take orally 10 days, during medication, there is not any bad or Deviant Behavior performance in healthy shrimp, the drinking-water of searching for food is normal, and body weight increases that to compare difference not remarkable with blank group (being not administration of health group).
The above-mentioned detailed description that pure Chinese medicine composition to this treatment fish and shrimp gill rot and preparation method thereof carries out with reference to embodiment; illustrative instead of determinate; can list several embodiment according to institute's limited range; therefore in the variation and the amendment that do not depart under general plotting of the present invention, within should belonging to protection scope of the present invention.
Claims (3)
1. treat a pure Chinese medicine composition for fish and shrimp gill rot, it is characterized in that by following component by weight mixed preparing form: Herba polygoni hydropiperis 15-35 part, Folium Artemisiae Argyi 15-35 part, Herba Artemisiae Annuae 15-35 part, propolis 15-35 part, Radix Tripterygii Wilfordii 15-35 part, Herba Houttuyniae 15-35 part, Radix Astragali 20-40 part, Pericarpium Citri Reticulatae 20-40 part, Radix Bupleuri 20-40 part, Rhizoma Chuanxiong 20-40 part, Radix Sophorae Tonkinensis 15-35 part, Radix Glycyrrhizae 20-40 part.
2. the pure Chinese medicine composition for the treatment of fish and shrimp gill rot according to claim 1, it is characterized in that by following component by weight mixed preparing form: 25 parts of Herba polygoni hydropiperis, 25 parts of Folium Artemisiae Argyis, 25 parts of Herba Artemisiae Annuaes, 25 parts of propolis, 25 parts of Radix Tripterygii Wilfordiis, 25 parts of Herba Houttuyniae, 30 parts of the Radixs Astragali, 30 parts of Pericarpium Citri Reticulataes, 30 parts of Radix Bupleuri, 30 parts of Rhizoma Chuanxiongs, 25 parts of Radix Sophorae Tonkinensiss, 30 parts, Radix Glycyrrhizae.
3. a preparation method for the pure Chinese medicine composition for the treatment of fish and shrimp gill rot according to claim 1, is characterized in that carrying out as follows:
(1) the each composition in prescription is crossed to 700 mesh sieves after micronizing respectively;
(2) take by weight Herba polygoni hydropiperis 15-35 part, Folium Artemisiae Argyi 15-35 part, Herba Artemisiae Annuae 15-35 part, propolis 15-35 part, Radix Tripterygii Wilfordii 15-35 part, Herba Houttuyniae 15-35 part, mix 5-10 minute to even;
(3) take by weight Radix Astragali 20-40 part, Pericarpium Citri Reticulatae 20-40 part, Radix Bupleuri 20-40 part, Rhizoma Chuanxiong 20-40 part, Radix Sophorae Tonkinensis 15-35 part, Radix Glycyrrhizae 20-40 part, mix 5-10 minute to even;
(4) merge the compositions that above-mentioned (2), (3) step make, continue to mix 10-15 minute to evenly and get final product.
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CN104257812A (en) * | 2014-09-29 | 2015-01-07 | 浙江海洋学院 | Fish medicine for treating branchiomycosis |
CN105918644A (en) * | 2015-10-16 | 2016-09-07 | 湖北文理学院 | Feed additive for control of goldfish fin rot disease and preparation method thereof |
CN106509486A (en) * | 2016-10-24 | 2017-03-22 | 广西智宝科技有限公司 | Crucian carp feed capable of preventing and treating branchia hyperemia disease and preparation method of crucian carp feed |
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CN1895419A (en) * | 2006-06-16 | 2007-01-17 | 天津生机集团有限公司 | Multiple Chinese-medicinal prescription for aquatic cultivation |
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CN102138994A (en) * | 2010-10-31 | 2011-08-03 | 仙桃市魏氏生物工程有限责任公司 | Pharmaceutical composition for preventing and treating black gill disease of aquatic animals |
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