CN100374556C - 脂解酶变体 - Google Patents

脂解酶变体 Download PDF

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Publication number
CN100374556C
CN100374556C CNB99813810XA CN99813810A CN100374556C CN 100374556 C CN100374556 C CN 100374556C CN B99813810X A CNB99813810X A CN B99813810XA CN 99813810 A CN99813810 A CN 99813810A CN 100374556 C CN100374556 C CN 100374556C
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CN
China
Prior art keywords
lipolytic enzyme
activity
amino acid
e1sppcgrrp
lipase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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CNB99813810XA
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English (en)
Chinese (zh)
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CN1331742A (zh
Inventor
柯尔斯滕·博杰森
阿伦·斯文德森
克劳斯·C·富格尔桑
阿南特·P·沙姆康特
基姆·博尔奇
杰斯珀·文达
安德烈亚斯·皮特里
桑尼·S·格拉德
吉特·布多尔夫森
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Novozymes AS
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Novozymes AS
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Publication of CN1331742A publication Critical patent/CN1331742A/zh
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Publication of CN100374556C publication Critical patent/CN100374556C/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
    • C12H1/02—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material
    • C12H1/04—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material with the aid of ion-exchange material or inert clarification material, e.g. adsorption material
    • C12H1/0408—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material with the aid of ion-exchange material or inert clarification material, e.g. adsorption material with the aid of inorganic added material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
    • C12H1/02—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material
    • C12H1/04—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material with the aid of ion-exchange material or inert clarification material, e.g. adsorption material
    • C12H1/0416—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material with the aid of ion-exchange material or inert clarification material, e.g. adsorption material with the aid of organic added material
    • C12H1/0424—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material with the aid of ion-exchange material or inert clarification material, e.g. adsorption material with the aid of organic added material with the aid of a polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Inorganic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Seasonings (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Dairy Products (AREA)
  • Fats And Perfumes (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
CNB99813810XA 1998-11-27 1999-11-29 脂解酶变体 Expired - Lifetime CN100374556C (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DKPA199801572 1998-11-27
DKPA199801572 1998-11-27
DKPA199900391 1999-03-22
DKPA199900391 1999-03-22
DKPA199901481 1999-10-15
DKPA199901481 1999-10-15

Publications (2)

Publication Number Publication Date
CN1331742A CN1331742A (zh) 2002-01-16
CN100374556C true CN100374556C (zh) 2008-03-12

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Family Applications (1)

Application Number Title Priority Date Filing Date
CNB99813810XA Expired - Lifetime CN100374556C (zh) 1998-11-27 1999-11-29 脂解酶变体

Country Status (11)

Country Link
EP (10) EP2302044A1 (cg-RX-API-DMAC10.html)
JP (3) JP4723087B2 (cg-RX-API-DMAC10.html)
CN (1) CN100374556C (cg-RX-API-DMAC10.html)
AT (1) ATE441704T1 (cg-RX-API-DMAC10.html)
AU (2) AU785464B2 (cg-RX-API-DMAC10.html)
BR (1) BRPI9915711B1 (cg-RX-API-DMAC10.html)
CA (2) CA2715086A1 (cg-RX-API-DMAC10.html)
DE (1) DE69941364D1 (cg-RX-API-DMAC10.html)
DK (1) DK2290059T3 (cg-RX-API-DMAC10.html)
ES (1) ES2328429T3 (cg-RX-API-DMAC10.html)
TR (1) TR200101497T2 (cg-RX-API-DMAC10.html)

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EP2302044A1 (en) * 1998-11-27 2011-03-30 Novozymes A/S Lipolytic enzyme variants
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CN102533440B (zh) * 2004-07-16 2017-09-19 杜邦营养生物科学有限公司 用酶使油脱胶的方法
WO2006133698A2 (en) * 2005-06-13 2006-12-21 Novozymes A/S Production of degummed fatty acid alkyl esters
CA2612648A1 (en) * 2005-06-24 2006-12-28 Novozymes A/S Lipases for pharmaceutical use
JP2009523461A (ja) * 2006-01-23 2009-06-25 ザ プロクター アンド ギャンブル カンパニー 洗剤組成物
CN110093330B (zh) * 2012-10-12 2023-11-28 丹尼斯科美国公司 包含脂解酶变体的组合物和方法
CN105814199B (zh) 2013-12-10 2019-12-13 天野酶株式会社 修饰型脂肪酶及其用途
EP3149178B1 (en) 2014-05-27 2020-07-15 Novozymes A/S Lipase variants and polynucleotides encoding same
CN104293744B (zh) * 2014-08-19 2017-04-12 浙江工业大学 来源于嗜热踝节菌的脂肪酶突变体及应用
EP3227442B1 (en) * 2014-12-05 2022-02-16 Novozymes A/S Lipase variants and polynucleotides encoding same
CA2965232A1 (en) * 2014-12-09 2016-06-16 Novozymes A/S Lipase variants and polynucleotides encoding same
CA2966292C (en) * 2014-12-22 2022-10-04 Novozymes A/S Detergent compositions, lipase variants and polynucleotides encoding same
JP7058227B2 (ja) * 2017-02-06 2022-04-21 株式会社日清製粉ウェルナ パン粉ミックス
CN108497298A (zh) * 2017-02-27 2018-09-07 诺维信公司 一种酶组合物及其应用
CN108497462A (zh) * 2017-02-27 2018-09-07 诺维信公司 一种含脂肪酶的面粉改良剂及应用
AU2018383752B2 (en) * 2017-12-13 2025-01-30 Codexis, Inc. Carboxyesterase polypeptides for amide coupling
JP2019165727A (ja) * 2018-03-22 2019-10-03 物産フードサイエンス株式会社 ドウにおけるリパーゼの新規用途
AU2019360247B2 (en) * 2018-10-17 2026-01-08 Perfect Day, Inc. Recombinant components and compositions for use in food products
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WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN114455204B (zh) * 2022-01-17 2023-09-29 黑龙江省绿色食品科学研究院 一种复合降血压多肽可食性包装膜的制备方法
JP2023119721A (ja) * 2022-02-17 2023-08-29 物産フードサイエンス株式会社 米飯のほぐれ、パラパラ感、粒感、硬さおよび油感を向上するための組成物ならびに米飯の製造方法

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