CA2993224A1 - Exosome compositions and use thereof for soft tissue repair - Google Patents
Exosome compositions and use thereof for soft tissue repair Download PDFInfo
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- CA2993224A1 CA2993224A1 CA2993224A CA2993224A CA2993224A1 CA 2993224 A1 CA2993224 A1 CA 2993224A1 CA 2993224 A CA2993224 A CA 2993224A CA 2993224 A CA2993224 A CA 2993224A CA 2993224 A1 CA2993224 A1 CA 2993224A1
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- exosomes
- heat shock
- stem cells
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| US11596656B2 (en) * | 2016-08-23 | 2023-03-07 | Albert Einstein College Of Medicine | Stem cell-produced microvesicles for treating tendon pathologies |
| CN107080753A (zh) * | 2017-04-20 | 2017-08-22 | 深圳市赛欧细胞生物科技有限公司 | 一种人脐带间充质干细胞源外泌体的美容制剂 |
| CN107006452A (zh) * | 2017-04-20 | 2017-08-04 | 深圳市赛欧细胞生物科技有限公司 | 一种人脐带间充质干细胞源外泌体冻干保存方法及其应用 |
| CN107245472B (zh) * | 2017-06-08 | 2021-08-06 | 北京智能宝生物科技有限公司 | 一种人间充质干细胞外泌体冻干粉的制备方法、使用方法 |
| WO2019004738A2 (ko) * | 2017-06-30 | 2019-01-03 | 주식회사 엑소코바이오 | 지방줄기세포 유래의 엑소좀을 유효성분으로 포함하는 조성물의 피부염 개선 용도 |
| WO2019004757A2 (ko) * | 2017-06-30 | 2019-01-03 | 주식회사 엑소코바이오 | 줄기세포 유래의 엑소좀을 유효성분으로 포함하는 조성물의 가려움증 억제 또는 개선 용도 |
| KR20190003316A (ko) | 2017-06-30 | 2019-01-09 | 주식회사 엑소코바이오 | 지방줄기세포 유래의 엑소좀 및/또는 세포외 소포체를 유효성분으로 포함하는 조성물의 피부염 개선 용도 |
| WO2019040790A1 (en) | 2017-08-23 | 2019-02-28 | Merakris Therapeutics, Llc | COMPOSITIONS CONTAINING AMNIOTIC COMPONENTS AND METHODS FOR THEIR PREPARATION AND USE |
| EP3681288B1 (en) * | 2017-09-11 | 2025-11-26 | Symbiocelltech, LLC | Adaptation of hollow-fiber-based cell culture technology for the manufacturing of neo-islets or exosomes from stem cells |
| CN111527199A (zh) | 2017-11-16 | 2020-08-11 | 得克萨斯大学体系董事会 | 用于制备来源于msc的外泌体的方法 |
| KR20190060646A (ko) | 2017-11-24 | 2019-06-03 | 주식회사 엑소코바이오 | 줄기세포 유래의 엑소좀 및/또는 세포외 소포체를 유효성분으로 포함하는 조성물의 피부장벽 강화 내지 기능 개선 용도 |
| WO2019103381A1 (ko) * | 2017-11-24 | 2019-05-31 | 주식회사 엑소코바이오 | 줄기세포 유래의 엑소좀을 유효성분으로 포함하는 조성물의 피부장벽 강화 내지 기능 개선 용도 |
| KR102039302B1 (ko) * | 2017-11-24 | 2019-10-31 | 주식회사 엑소코바이오 | 줄기세포 유래의 엑소좀을 유효성분으로 포함하는 조성물의 피부장벽 강화 내지 기능 개선 용도 |
| US12036325B2 (en) | 2017-12-14 | 2024-07-16 | Mayo Foundation For Medical Education And Research | Purified exosome products, method of making, and methods of using |
| WO2019139762A1 (en) * | 2018-01-09 | 2019-07-18 | Zen-Bio, Inc. | Exosome compositions and use thereof for joint disorders and diseases |
| JP7474702B2 (ja) | 2018-03-28 | 2024-04-25 | ボード オブ リージェンツ，ザ ユニバーシティ オブ テキサス システム | エキソソームから単離されたｄｎａにおけるエピジェネティック変化の同定法 |
| US20210244769A1 (en) * | 2018-05-04 | 2021-08-12 | Figene, Llc | Pain-reducing effects of fibroblasts and treatment of pain |
| RU2710368C2 (ru) * | 2018-05-29 | 2019-12-26 | Станислав Евгеньевич Волчков | Способ получения и концентрирования микроРНК-содержащих экзосом мультипотентных мезенхимально-стромальных клеток для применения в косметических и лекарственных средствах для стимуляции регенеративных процессов и замедления процессов старения |
| US12453743B2 (en) | 2018-05-30 | 2025-10-28 | Direct Biologics, Llc | Mesenchymal stem cell (MSC) growth factor and extracellular vesicle preparation in frozen or powdered form and methods of use |
| KR102646145B1 (ko) * | 2018-05-31 | 2024-03-11 | 주식회사 엑소코바이오 | 줄기세포 유래의 엑소좀을 유효성분으로 포함하는 모공 축소용 조성물 |
| WO2019231133A1 (ko) * | 2018-05-31 | 2019-12-05 | 주식회사 엑소코바이오 | 줄기세포 유래의 엑소좀을 유효성분으로 포함하는 모공 축소용 조성물 |
| CN108715834B (zh) * | 2018-06-01 | 2021-09-14 | 天晴干细胞股份有限公司 | 一种富含cd41+、cd81+微囊的血小板裂解液制备方法 |
| KR102176845B1 (ko) * | 2018-10-02 | 2020-11-10 | 주식회사 스템온 | 유도된 엑소좀을 포함하는 피부 재생 및 상처 치유용 조성물 |
| EP3930734A4 (en) | 2019-02-28 | 2022-12-07 | Merakris Therapeutics, LLC | EXTRACELLULAR VESICLES DERIVED FROM AMBIENT WATER AND THEIR USES IN WOUND HEALING |
| WO2020180311A1 (en) * | 2019-03-06 | 2020-09-10 | Zen-Bio, Inc. | Plant-based exosome compositions and use thereof for rejuvenating skin |
| CA3138177A1 (en) * | 2019-04-29 | 2020-11-05 | Direct Biologics Llc | Method for the treatment of periodontal disease using characterized mesenchymal stem cell growth factors and exosomes |
| EP3999083A4 (en) | 2019-07-18 | 2023-07-26 | Direct Biologics LLC | Preparations comprising mesenchymal stem cells and cannabinoids and methods of their use |
| JP2021040551A (ja) * | 2019-09-11 | 2021-03-18 | 富士フイルム株式会社 | 培地組成物、キット、間葉系幹細胞組成物の製造方法、間葉系幹細胞組成物、間葉系幹細胞、細胞培養上清および細胞外小胞 |
| CN110559254A (zh) * | 2019-10-20 | 2019-12-13 | 张新梅 | 一种外泌体组合物、其制备方法及应用 |
| CA3160078A1 (en) * | 2019-11-04 | 2021-05-14 | Omar Mohamed Salah Eldin Ahmed ELTOOKHY | Method for coating and drying heterogenous stem cell derived extra-cellular vesicles |
| CN110721198A (zh) * | 2019-11-08 | 2020-01-24 | 赵凯 | 一种预防癫痫产生脑损伤的外泌体喷雾的制作工艺 |
| WO2021147922A1 (zh) | 2020-01-20 | 2021-07-29 | 医微细胞生物技术(广州)有限公司 | 细胞外囊泡及其在皮肤产品的用途 |
| CN113134014B (zh) * | 2020-01-20 | 2023-09-26 | 医微细胞生物技术(广州)有限公司 | 囊泡在制备血友病药物中的应用 |
| US12077780B2 (en) | 2020-02-14 | 2024-09-03 | Allergan Sales, Llc | Conditioned medium from cells cultured under hypoxic conditions and uses thereof |
| US20240390551A1 (en) * | 2021-01-20 | 2024-11-28 | Research & Business Foundation Sungkyunkwan University | Filler composition for reducing skin wrinkles comprising stem cell-derived exosomes, hyaluronic acid, and bdde and method for preparing same |
| KR102368442B1 (ko) * | 2021-02-19 | 2022-02-28 | 주식회사 엑소코바이오 | 생리활성이 강화된 엑소좀의 생산방법 및 이의 응용 |
| TW202235614A (zh) * | 2021-03-11 | 2022-09-16 | 長新生醫國際股份有限公司 | 幹細胞凍乾粉之製造方法 |
| CN113041259B (zh) * | 2021-03-23 | 2023-05-12 | 哈尔滨科技实业开发有限公司 | 牙髓干细胞外泌体制剂及制备方法和应用 |
| CA3213489A1 (en) * | 2021-03-31 | 2022-10-06 | Giorgio Dell'acqua | Compositions comprising plant-derived exosome-like nanovesicles or exosomes and methods of use thereof |
| CN113384518A (zh) * | 2021-07-27 | 2021-09-14 | 山西遗传资源细胞库有限公司 | 一种干细胞外泌体应用于面膜的方法 |
| JPWO2023079655A1 (enExample) * | 2021-11-04 | 2023-05-11 | ||
| CN113940912B (zh) * | 2021-12-09 | 2024-04-16 | 深圳市泓浩生物科技有限公司 | 抗衰老的组合物及其应用 |
| CN116099056A (zh) * | 2023-04-07 | 2023-05-12 | 云南云科特色植物提取实验室有限公司 | 一种含仿生纹理结构的人工皮肤及其制备方法 |
| CN116410918B (zh) * | 2023-06-12 | 2023-08-25 | 成都诺医德医学检验实验室有限公司 | 一种皮肤类器官外泌体及其制备方法和应用 |
| WO2025030027A1 (en) * | 2023-08-03 | 2025-02-06 | Wisconsin Alumni Research Foundation | Extracellular vesicle therapy to treat orthopedic injury |
| CN118576721A (zh) * | 2024-05-27 | 2024-09-03 | 深圳市中安康华生物科技有限公司 | 一种外泌体复合生物制剂及其制备方法与应用 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5348945A (en) * | 1990-04-06 | 1994-09-20 | Wake Forest University | Method of treatment with hsp70 |
| US5593682A (en) * | 1995-12-29 | 1997-01-14 | Eastman Chemical Company | Skin treating composition |
| AT409379B (de) * | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
| JP2004331602A (ja) * | 2003-05-09 | 2004-11-25 | Arysta Lifescience Corp | 皮膚外用剤 |
| EP1514539A3 (en) * | 2003-05-14 | 2005-05-04 | Signal Investment & Management Co. | Micro-encapsulated topical analgesic for pain relief and sleeve comprising it |
| US20060143782A1 (en) * | 2004-10-07 | 2006-07-06 | Sherry Ajluni | Glove with skin lotion |
| US8022037B2 (en) * | 2007-01-08 | 2011-09-20 | University Of Southern California | Skin wound healing compositions and methods of use thereof |
| US20110003008A1 (en) * | 2008-02-22 | 2011-01-06 | Agency For Science, Technology And Research (A*Star) | Mesenchymal stem cell particles |
| CN103118691B (zh) * | 2010-08-23 | 2016-08-24 | 康干细胞生物科技有限公司 | 预防和治疗免疫病变和炎性疾病的包括用nod2激动剂处理的干细胞或其培养物的药物组合物 |
| JP5911874B2 (ja) * | 2010-10-18 | 2016-04-27 | エージェンシー フォー サイエンス，テクノロジー アンド リサーチ | 発毛を促進又は増強するためのエキソソームの使用 |
| US9119974B2 (en) * | 2011-03-04 | 2015-09-01 | Ahmed H. Al-Qahtani | Skin cream |
| CN109432126B (zh) * | 2011-03-11 | 2022-06-14 | 儿童医学中心公司 | 与间充质干细胞外来体相关的方法和组合物 |
| JP3171007U (ja) * | 2011-06-28 | 2011-10-13 | ヘルスアシスト株式会社 | 腕肩美容パックシート |
| US9427450B2 (en) * | 2012-01-31 | 2016-08-30 | Xon Cells, Inc. | Therapeutic immune modulation by stem cell secreted exosomes |
| EP2687219A1 (en) * | 2012-07-18 | 2014-01-22 | Universität Duisburg-Essen | Use of preparations comprising exosomes derived from mesenchymal stem cells (MSCs) in the prevention and therapy of inflammatory conditions |
| CN105209881B (zh) * | 2013-03-13 | 2021-01-22 | 迈阿密大学 | 从细胞培养上清液和生物流体分离并纯化微泡的方法 |
| WO2014197747A1 (en) * | 2013-06-05 | 2014-12-11 | The Trustees Of Columbia University In The City Of New York | Exosomes for orofacial diagnostics and therapeutics |
| JP6253361B2 (ja) * | 2013-11-21 | 2017-12-27 | ポーラ化成工業株式会社 | 皮膚外用剤 |
| JP2015101541A (ja) * | 2013-11-21 | 2015-06-04 | ポーラ化成工業株式会社 | 皮膚関連酵素活性化剤 |
| US9464272B2 (en) * | 2013-12-12 | 2016-10-11 | Stemnion, Inc. | Cell-derived composition |
| CN104488850B (zh) * | 2014-11-28 | 2016-11-02 | 广州赛莱拉干细胞科技股份有限公司 | 一种制备人羊膜间充质干细胞外泌体冻干粉的方法 |
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| EP3328403A4 (en) | 2019-04-17 |
| JP2018522593A (ja) | 2018-08-16 |
| JP2018522071A (ja) | 2018-08-09 |
| JP6980955B2 (ja) | 2021-12-15 |
| WO2017023690A1 (en) | 2017-02-09 |
| EP3328397A4 (en) | 2019-04-03 |
| SMT202300249T1 (it) | 2023-09-06 |
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