BRPI9612938B8 - método para cultivo de lawsonia intracellularis em células mccoy, bem como uso das referidas células. - Google Patents

método para cultivo de lawsonia intracellularis em células mccoy, bem como uso das referidas células.

Info

Publication number
BRPI9612938B8
BRPI9612938B8 BRPI9612938A BRPI9612938A BRPI9612938B8 BR PI9612938 B8 BRPI9612938 B8 BR PI9612938B8 BR PI9612938 A BRPI9612938 A BR PI9612938A BR PI9612938 A BRPI9612938 A BR PI9612938A BR PI9612938 B8 BRPI9612938 B8 BR PI9612938B8
Authority
BR
Brazil
Prior art keywords
bacteria
cells
infected cells
attenuated
cultivate
Prior art date
Application number
BRPI9612938A
Other languages
English (en)
Portuguese (pt)
Inventor
P Knittel Jeffrey
B Roof Michael
Original Assignee
Boehringer Ingelheim Vetmedica Inc
Boehringer Ingelheim/Nobl Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27041284&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI9612938(B8) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US08/465,337 external-priority patent/US5714375A/en
Application filed by Boehringer Ingelheim Vetmedica Inc, Boehringer Ingelheim/Nobl Laboratories Inc filed Critical Boehringer Ingelheim Vetmedica Inc
Publication of BRPI9612938B1 publication Critical patent/BRPI9612938B1/pt
Publication of BRPI9612938B8 publication Critical patent/BRPI9612938B8/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/105—Delta proteobacteriales, e.g. Lawsonia; Epsilon proteobacteriales, e.g. campylobacter, helicobacter
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56922—Campylobacter
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/521—Bacterial cells; Fungal cells; Protozoal cells inactivated (killed)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
    • A61K2039/552—Veterinary vaccine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S424/00—Drug, bio-affecting and body treating compositions
    • Y10S424/825—Bacterial vaccine for porcine species, e.g. swine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Food Science & Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Oncology (AREA)
BRPI9612938A 1995-06-05 1996-06-05 método para cultivo de lawsonia intracellularis em células mccoy, bem como uso das referidas células. BRPI9612938B8 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/465,337 US5714375A (en) 1995-06-05 1995-06-05 Ileal symbiont intracellularis propagation in suspended host cells
US08/658,194 US5885823A (en) 1995-06-05 1996-06-04 Lawsonia intracellularis cultivation, anti-Lawsonia intracellularis vaccines and diagnostic agents
PCT/US1996/009576 WO1996039629A1 (en) 1995-06-05 1996-06-05 Lawsonia intracellularis cultivation, anti-lawsonia intracellularis vaccines and diagnostic agents
BRPI9608338-7A BR9608338B1 (pt) 1995-06-05 1996-06-05 vacina para a indução de uma resposta imune a bactérias de l-intracelularis em um animal não humano.

Publications (2)

Publication Number Publication Date
BRPI9612938B1 BRPI9612938B1 (pt) 2010-07-27
BRPI9612938B8 true BRPI9612938B8 (pt) 2017-09-12

Family

ID=27041284

Family Applications (2)

Application Number Title Priority Date Filing Date
BRPI9612938A BRPI9612938B8 (pt) 1995-06-05 1996-06-05 método para cultivo de lawsonia intracellularis em células mccoy, bem como uso das referidas células.
BRPI9608338-7A BR9608338B1 (pt) 1995-06-05 1996-06-05 vacina para a indução de uma resposta imune a bactérias de l-intracelularis em um animal não humano.

Family Applications After (1)

Application Number Title Priority Date Filing Date
BRPI9608338-7A BR9608338B1 (pt) 1995-06-05 1996-06-05 vacina para a indução de uma resposta imune a bactérias de l-intracelularis em um animal não humano.

Country Status (23)

Country Link
US (1) US5885823A (cg-RX-API-DMAC7.html)
EP (5) EP1655607B1 (cg-RX-API-DMAC7.html)
JP (4) JP3765834B2 (cg-RX-API-DMAC7.html)
KR (4) KR100758614B1 (cg-RX-API-DMAC7.html)
AT (3) ATE289069T1 (cg-RX-API-DMAC7.html)
AU (1) AU717045B2 (cg-RX-API-DMAC7.html)
BG (1) BG64292B1 (cg-RX-API-DMAC7.html)
BR (2) BRPI9612938B8 (cg-RX-API-DMAC7.html)
CA (1) CA2222643C (cg-RX-API-DMAC7.html)
CZ (1) CZ295933B6 (cg-RX-API-DMAC7.html)
DE (5) DE69634334T2 (cg-RX-API-DMAC7.html)
DK (3) DK1403643T3 (cg-RX-API-DMAC7.html)
ES (3) ES2173055T3 (cg-RX-API-DMAC7.html)
FR (1) FR07C0071I2 (cg-RX-API-DMAC7.html)
HU (1) HU230199B1 (cg-RX-API-DMAC7.html)
LU (1) LU91405I2 (cg-RX-API-DMAC7.html)
NL (1) NL300331I2 (cg-RX-API-DMAC7.html)
NZ (1) NZ319994A (cg-RX-API-DMAC7.html)
PL (6) PL187850B1 (cg-RX-API-DMAC7.html)
PT (3) PT1645567E (cg-RX-API-DMAC7.html)
RO (1) RO118885B1 (cg-RX-API-DMAC7.html)
RU (2) RU2265849C2 (cg-RX-API-DMAC7.html)
WO (1) WO1996039629A1 (cg-RX-API-DMAC7.html)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7022328B1 (en) 1995-11-30 2006-04-04 Australian Pork Limited Therapeutic and diagnostic compositions
FR2772047B1 (fr) 1997-12-05 2004-04-09 Ct Nat D Etudes Veterinaires E Sequence genomique et polypeptides de circovirus associe a la maladie de l'amaigrissement du porcelet (map), applications au diagnostic et a la prevention et/ou au traitement de l'infection
BR0011292A (pt) * 1999-05-13 2002-02-26 Pfizer Prod Inc Gene derivado de lawsonia e proteìnas, peptìdeos e polipeptìdeos sodc relacionados e seus usos
US7029683B1 (en) 1999-05-13 2006-04-18 Pfizer Products, Inc. Lawsonia derived gene and related hemolysin polypeptides, peptides and proteins and their uses
US7052697B1 (en) * 1999-05-13 2006-05-30 Agriculture Victoria Services Pty Ltd Lawsonia derived gene and related OmpH polypeptides, peptides and proteins and their uses
CA2372102A1 (en) * 1999-05-13 2000-11-23 Australian Pork Limited Lawsonia derived gene and related omph polypeptides, peptides and proteins and their uses
BR0011294A (pt) * 1999-05-13 2002-02-26 Pfizer Prod Inc Gene derivado lawsonia e polipeptìdeos, peptìdeos e proteìnas flge relacionados e seus usos
NZ515363A (en) * 1999-05-13 2003-07-25 Pfizer Prod Inc Lawsonia derived gene and related hemolysin polypeptides, peptides and proteins and their uses
US6605696B1 (en) * 1999-10-22 2003-08-12 Pfizer, Inc. Lawsonia intracellularis proteins, and related methods and materials
AU9315101A (en) * 2000-09-29 2002-04-08 Univ Arizona Improved vaccines for proliferative ileitis and methods of making and using the same
AUPR138100A0 (en) 2000-11-10 2000-12-07 Agriculture Victoria Services Pty Ltd Novel therapeutic compositions for treating infection by lawsonia spp
NZ520600A (en) * 2000-11-10 2004-08-27 Agriculture Victoria Serv Pty Novel therapeutic compositions for treating infection by Lawsonia spp
ATE501258T1 (de) * 2000-12-20 2011-03-15 Intervet Int Bv Lawsonia intracellularis impfstoff
US20030087421A1 (en) * 2001-07-11 2003-05-08 Gebhart Connie J Lawsonia intracellularis
JP2006501846A (ja) * 2002-10-04 2006-01-19 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ ローソニア・イントラセルラリス由来の核酸およびポリペプチド配列ならびに使用方法
UA87665C2 (ru) * 2003-07-25 2009-08-10 Берингер Ингельхайм Ветмедика, Инк. Авирулентный изолят lawsonia intracellularis европейского происхождения, вакцина для иммунизации животных, применение заявленного авирулентного изолята как вакцины, способ получения вакцины, способ культивирования lawsonia intracellularis, способ диагностики пролиферативной энтеропатии свиней и способ получения антисыворотки к lawsonia intracellularis
CA2536989A1 (en) 2003-09-12 2005-03-24 Akzo Nobel N.V. Lawsonia intracellularis subunit vaccine
WO2005056586A1 (en) * 2003-12-09 2005-06-23 Intervet International B.V Lawsonia intracellularis 26 kd subunit vaccine
JP2007537721A (ja) * 2004-01-22 2007-12-27 インターベツト・インターナシヨナル・ベー・ベー ローソニア・イントラセルラーリスのサブユニットワクチン
EP1609870A1 (en) * 2004-06-24 2005-12-28 Boehringer Ingelheim Vetmedica Gmbh Method of diagnosing lawsonia intracellularis
MY146476A (en) * 2004-06-24 2012-08-15 Boehringer Ingelheim Vetmed Method of diagnosing lawsonia intracellularis
US20060021536A1 (en) * 2004-07-30 2006-02-02 Limin Song Method for creating an absorbent article exhibiting a harmonic color scheme
KR100621882B1 (ko) * 2004-09-30 2006-09-19 건국대학교 산학협력단 돼지 증식성 회장염 감염돈에서 로소니아인트라셀룰라리스 균을 분리하는 방법
CA2586093A1 (en) * 2004-11-24 2006-06-01 Pharmacia & Upjohn Company Llc Methods for cultivating lawsonia intracellularis
US20070212373A1 (en) * 2004-12-08 2007-09-13 Akzo Nobel N.V. Lawsonia Intracellularis 26 Kd Subunit Vaccine
US7833707B2 (en) 2004-12-30 2010-11-16 Boehringer Ingelheim Vetmedica, Inc. Methods of overexpression and recovery of porcine circovirus type 2 ORF2
US7700285B1 (en) 2005-12-29 2010-04-20 Boehringer Ingelheim Vetmedica, Inc. PCV2 immunogenic compositions and methods of producing such compositions
UA95602C2 (ru) 2004-12-30 2011-08-25 Берингер Ингельхейм Ветмедика, Инк. Иммуногенная композиция цвс2 и способы приготовления такой композиции
US8834891B2 (en) 2005-03-14 2014-09-16 Boehringer Ingelheim Vetmedica, Inc. Immunogenic compositions comprising Lawsonia intracellularis
US8398994B2 (en) 2005-07-15 2013-03-19 Boehringer Ingelheim Vetmedica, Inc. Lawsonia vaccine and methods of use thereof
MY180002A (en) 2005-12-29 2020-11-19 Boehringer Ingelheim Vetmedica Inc Multivalent pcv2 immunogenic compositions and methods of producing compositions
CN101426522B (zh) 2005-12-29 2014-11-12 贝林格尔.英格海姆维特梅迪卡有限公司 Pcv2免疫原性组合物用于减轻猪临床症状的用途
US8470336B2 (en) * 2006-05-25 2013-06-25 Boehringer Ingelheim Vetmedica, Inc. Vaccination of young animals against Lawsonia intracellularis infections
US20080241190A1 (en) * 2006-11-13 2008-10-02 Boehringer Ingelheim Vetmedica, Inc. Vaccination of horses against lawsonia intracellularis
EP2859900A1 (en) 2006-12-11 2015-04-15 Boehringer Ingelheim Vetmedica, Inc. Effective method of treatment of porcine circovirus and lawsonia intracellularis infections
WO2008076915A2 (en) * 2006-12-15 2008-06-26 Boehringer Ingelheim Vetmedica, Inc. Treatment of pigs with pcv2 antigen
EP1941903A1 (en) 2007-01-03 2008-07-09 Boehringer Ingelheim Vetmedica Gmbh Prophylaxis and treatment of PRDC
EP1958644A1 (en) * 2007-02-13 2008-08-20 Boehringer Ingelheim Vetmedica Gmbh Prevention and treatment of sub-clinical pcvd
US7829274B2 (en) 2007-09-04 2010-11-09 Boehringer Ingelheim Vetmedica, Inc. Reduction of concomitant infections in pigs by the use of PCV2 antigen
WO2009037262A2 (en) 2007-09-17 2009-03-26 Boehringer Ingelheim Vetmedica, Inc. Method of preventing early lawsonia intracellularis infections
JP2011500034A (ja) * 2007-10-12 2011-01-06 ファイザー・インク ロウソニア・イントラセルラリス（ｌａｗｓｏｎｉａｉｎｔｒａｃｅｌｌｕｌａｒｉｓ）を培養する方法
CN101932336B (zh) * 2007-12-31 2014-07-09 贝林格尔.英格海姆维特梅迪卡有限公司 有外来氨基酸插入的pcv2 orf2病毒样颗粒
EP2242511A4 (en) 2008-01-23 2012-10-24 Boehringer Ingelheim Vetmed IMMUNOGENIC COMPOSITIONS OF MYCOPLASMA HYOPNEUMONIAE PCV2, AND METHODS FOR PRODUCING SUCH COMPOSITIONS
US20110033495A1 (en) * 2008-02-15 2011-02-10 Boehringer Ingelheim Vetmedica, Inc. Methods and compositions for reducing the impact of enteric diseases
TWI551295B (zh) 2008-04-18 2016-10-01 英特威特國際股份有限公司 防備胞內勞森菌（Ｌａｗｓｏｎｉａ ｉｎｔｒａｃｅｌｌｕｌａｒｉｓ）用疫苗
TWI449533B (zh) 2008-04-18 2014-08-21 Intervet Int Bv 防備胞內勞森菌（Ｌａｗｓｏｎｉａ ｉｎｔｒａｃｅｌｌｕｌａｒｉｓ）、豬肺炎黴漿菌（Ｍｙｃｏｐｌａｓｍａ ｈｙｏｐｎｅｕｍｏｎｉａｅ）及豬環狀病毒（Ｐｏｒｃｉｎｅ ｃｉｒｃｏ ｖｉｒｕｓ）用疫苗
ES2534556T3 (es) * 2008-07-22 2015-04-24 Intervet International Bv Bacteria Lawsonia intracellularis de un nuevo serotipo, vacuna basada en esa bacteria, anticuerpos adecuados para diagnosticar el nuevo serotipo de Lawsonia intracellularis e hibridomas para producir dichos anticuerpos
US8142760B2 (en) * 2008-09-05 2012-03-27 Nathan Len Winkelman Vaccination for Lawsonia intracellularis
WO2011006880A1 (en) * 2009-07-14 2011-01-20 Intervet International B.V. Lawsonia intracellularis propagation
AR078253A1 (es) * 2009-09-02 2011-10-26 Boehringer Ingelheim Vetmed Metodos para reducir la actividad antivirica en composiciones pcv-2 y composiciones pcv-2 con mejor inmunogenicidad
ES2606131T3 (es) 2009-11-09 2017-03-22 Intervet International B.V. Método para cultivar bacterias Lawsonia intracellularis en células McCoy infectadas de forma persistente
EP2938747B1 (en) 2012-12-28 2019-05-01 Boehringer Ingelheim Vetmedica GmbH Method of making a mycoplasma vaccine
CN105579060B (zh) 2013-09-25 2021-03-16 硕腾服务有限责任公司 Pcv2b趋异株疫苗组合物以及使用方法
WO2015051099A1 (en) 2013-10-02 2015-04-09 Boehringer Ingelheim Vetmedica, Inc. Pcv2 orf2 protein variant and virus like particles composed thereof
US10751405B2 (en) 2015-02-04 2020-08-25 Intervet Inc. Vaccine for use against subclinical Lawsonia infection in a pig
ES2798282T3 (es) * 2015-02-04 2020-12-10 Intervet Int Bv Una vacuna para usar contra la infección asintomática por Lawsonia en un cerdo
KR20180062468A (ko) * 2016-11-18 2018-06-11 녹십자수의약품(주) 신규한 로소니아 인트라셀룰라리스 균주 및 이의 용도
EP3758740A1 (en) 2018-02-28 2021-01-06 Boehringer Ingelheim Vetmedica GmbH Method for reducing skatole and/or indole in animals
KR20230044474A (ko) 2020-07-24 2023-04-04 베링거 인겔하임 애니멀 헬스 유에스에이 인크. 조합 돼지 백신
EP4426342A1 (en) 2021-11-01 2024-09-11 Boehringer Ingelheim Vetmedica GmbH Immunogenic gel compositions
WO2024068637A1 (en) 2022-09-27 2024-04-04 Intervet International B.V. A method of culturing lawsonia intracellularis bacteria
WO2024260953A1 (en) * 2023-06-23 2024-12-26 Intervet International B.V. A method of culturing lawsonia intracellularis bacteria
CN119185351B (zh) * 2024-09-25 2025-09-16 武汉轻工大学 Li0004蛋白靶点在制备抑制劳森菌感染细胞增殖的药物中的应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4132597A (en) * 1976-06-09 1979-01-02 Ab Medipharm Method for cultivation of bacteria
US4237218A (en) * 1979-02-09 1980-12-02 Bio-Rad Laboratories, Inc. Micro-carrier cell culture
JPS5991879A (ja) * 1982-11-16 1984-05-26 Hideaki Yamada シユ−ドモナス属細菌の培養法
DK594084A (da) * 1984-12-12 1986-06-13 Novo Industri As Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning
JPH0644865B2 (ja) * 1985-10-29 1994-06-15 三菱瓦斯化学株式会社 カンピロバクタ−属細菌の培養方法
US5338670A (en) * 1986-07-28 1994-08-16 The United States Of America As Represented By The Department Of Health And Human Services Production of bordetella pertussis toxin with a low concentration of iron
IT1227657B (it) * 1988-12-01 1991-04-23 Mini Ricerca Scient Tecnolog Antibiotici ab-021 e processo per la loro produzione
JPH0822221B2 (ja) * 1988-12-28 1996-03-06 日東化学工業株式会社 シュードモナス属細菌の培養法
JPH02308754A (ja) * 1989-05-24 1990-12-21 Yakult Honsha Co Ltd 乳発酵食品の製造法
US5296221A (en) * 1990-01-31 1994-03-22 Sani-Ei Sucrochemical Co., Ltd. Lactobacillus johnsonii ferm bp-2680 lactic acid bacteria preparations using the same and a process of manufacturing the preparations
WO1992007934A1 (en) * 1990-11-01 1992-05-14 Iowa State University Research Foundation, Inc. Bacterial attenuation method and vaccine
SU1768637A1 (en) * 1990-12-04 1992-10-15 Inst Virusologii Im D I Ivanov Strain of human immunodeficient virus 1-type for preparation of diagnostic preparation
CA2123222C (en) * 1991-11-15 2001-02-27 Donald A. Dearwester Gram-negative bacterial vaccines
DE4208785A1 (de) * 1992-03-19 1993-09-23 Degussa Verfahren zum auffinden von insertionselementen (is-elemente) oder transposonen
US5610059A (en) * 1992-11-09 1997-03-11 University Of Arizona Etiological agent for porcine enteritis

Also Published As

Publication number Publication date
PT1403643E (pt) 2009-10-08
RU2305707C2 (ru) 2007-09-10
KR20050048651A (ko) 2005-05-24
PT1645567E (pt) 2007-11-19
JP2007151554A (ja) 2007-06-21
EP1645567B1 (en) 2007-09-12
ES2294620T3 (es) 2008-04-01
ATE441863T1 (de) 2009-09-15
JP3920300B2 (ja) 2007-05-30
EP2199795A2 (en) 2010-06-23
RO118885B1 (ro) 2003-12-30
JP3765834B2 (ja) 2006-04-12
BG64292B1 (bg) 2004-08-31
KR20060129549A (ko) 2006-12-15
DK0843818T3 (da) 2005-05-09
DE69637251T3 (de) 2016-06-02
JP2006061165A (ja) 2006-03-09
AU717045B2 (en) 2000-03-16
WO1996039629A1 (en) 1996-12-12
PT843818E (pt) 2005-04-29
FR07C0071I2 (fr) 2010-12-31
DE843818T1 (de) 2002-11-28
EP1403643B2 (en) 2018-10-03
EP1655607B1 (en) 2015-05-13
ES2294620T5 (es) 2016-03-23
PL189287B1 (pl) 2005-07-29
JPH11507225A (ja) 1999-06-29
HK1010911A1 (en) 1999-07-02
DE69638016D1 (de) 2009-10-15
JP4263212B2 (ja) 2009-05-13
CZ295933B6 (cs) 2005-12-14
EP2199795A3 (en) 2010-11-03
BR9608338B1 (pt) 2009-05-05
CA2222643C (en) 2013-08-13
KR100513481B1 (ko) 2006-01-27
EP0843818A1 (en) 1998-05-27
DK1645567T3 (da) 2007-11-26
ATE289069T1 (de) 2005-02-15
NL300331I1 (nl) 2008-03-03
MX9709464A (es) 1998-06-30
KR20050046741A (ko) 2005-05-18
EP1645567A1 (en) 2006-04-12
LU91405I2 (fr) 2008-02-21
PL189187B1 (pl) 2005-07-29
PL189286B1 (pl) 2005-07-29
DK1645567T4 (en) 2016-04-11
DK1403643T3 (da) 2010-01-04
CZ389997A3 (cs) 1998-07-15
ES2173055T3 (es) 2005-07-01
FR07C0071I1 (cg-RX-API-DMAC7.html) 2008-01-02
EP2199795B1 (en) 2016-03-30
BRPI9612938B1 (pt) 2010-07-27
HUP9900884A3 (en) 2001-01-29
NZ319994A (en) 2000-01-28
KR100544857B1 (ko) 2006-01-24
EP1655607A1 (en) 2006-05-10
PL189250B1 (pl) 2005-07-29
EP0843818B1 (en) 2005-02-09
EP1403643A1 (en) 2004-03-31
DE69637251T2 (de) 2008-06-12
BG102130A (bg) 1998-09-30
JP5078740B2 (ja) 2012-11-21
KR100758614B1 (ko) 2007-09-13
RU2265849C2 (ru) 2005-12-10
PL187850B1 (pl) 2004-10-29
DE122007000102I1 (de) 2008-04-17
ES2173055T1 (es) 2002-10-16
AU6262796A (en) 1996-12-24
JP2008266340A (ja) 2008-11-06
PL187849B1 (pl) 2004-10-29
EP1645567B2 (en) 2016-01-06
RU2005114739A (ru) 2006-11-20
HU230199B1 (hu) 2015-10-28
ATE373015T1 (de) 2007-09-15
EP1403643B1 (en) 2009-09-02
DE69634334D1 (de) 2005-03-17
BR9608338A (pt) 1999-01-05
EP0843818A4 (en) 2002-02-06
PL325872A1 (en) 1998-08-17
DE69637251D1 (de) 2007-10-25
CA2222643A1 (en) 1996-12-12
ES2332587T3 (es) 2010-02-09
NL300331I2 (nl) 2008-07-01
EP0843818B2 (en) 2018-12-19
KR19990022509A (ko) 1999-03-25
US5885823A (en) 1999-03-23
HUP9900884A2 (hu) 1999-07-28
DE69634334T2 (de) 2006-04-13

Similar Documents

Publication Publication Date Title
ES2294620T5 (es) Cultivo de Lawsonia intracellularis, vacunas anti-Lawsonia intracellularis y agentes de diagnóstico
ES2090297T3 (es) Produccion de proteinas que utilizan recombinacion homologa.
MY134726A (en) Lawsonia intracellularis cultivation, anti-lawsonia intracellularis vaccines and diagnostic agents
AU634148B2 (en) Use of cellulase preparations in the cultivation and use of cellulose-producing microorganisms
TH112991A (th) การเลี้ยง Lawsonia intracellularis, วัคซีนต้าน Lawsonia intracellularis และสารสำหรับใช้วินิจฉัย
JPS5743684A (en) Preparation of maltose dehydrogenase
JPS57132880A (en) Preparation of choline oxidase by fermentation method
KR880002995A (ko) 푸럭토 올리고(Fructo-oligo)당의 제조방법
Byalebeka Effect of the host genotype on Bradyrhizobium acceptance and performance on peanuts (Arachis hypogaea L.)
KR940014768A (ko) 복령재배방법
JPS55131391A (en) Preparation of heat-resistant cellulase
JPS5786292A (en) Preparation of n-benzoyl-l-alanine acylase
GB1110710A (en) Method of cultivating leishmania
JPS5678585A (en) Cultivation of phototrophic bacterium
JPS56106589A (en) Preparation of glutathione reductase
JPS56160990A (en) Preparation of yeast fungal body
DK529687A (da) Fremgangsmaade til fremstilling af l-arginin ved fermentering
JPS57206386A (en) Preparation of ethanolamine oxidase
JPS57206381A (en) Variant strain of brevibacterium ammoniagenes
GB951559A (en) Newcastle disease vaccine and the preparation thereof

Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07B Technical examination (opinion): publication cancelled [chapter 7.2 patent gazette]

Free format text: ANULADA A PUBLICACAO POR TER SIDO INDEVIDA REF A RPI 1962

B25A Requested transfer of rights approved

Owner name: BOEHRINGER INGELHEIM VETMEDICA, INC. (US)

Free format text: TRANSFERIDO POR FUSAO DE: BOEHRINGER INGELHEIM/NOBL LABORATORIES, INC.

B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 27/07/2010, OBSERVADAS AS CONDICOES LEGAIS.

B24C Patent annual fee: request for for restoration

Free format text: REFERENTE A 10A ANUIDADE.

B24E Patent annual fee: resolution cancelled

Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 2074 DE 05/10/2010.

B22O Other matters related to patents and certificates of addition of invention: legal action concerning patent

Free format text: INPI-52400.061189/2013 ORIGEM: JUIZO DA 013A VARA FEDERAL DO RIO DE JANEIRO PROCESSO NO 0132256-78.2013.4.02.5101 ACAO DE NULIDADE DAS PATENTES SUBMETIDAS AO MAILBOX (ART. 229, PARAGRAFO UNICO DA LPI); ALTERNATIVAMENTE, A DECRETACAO DA NULIDADE PARCIAL PARA CORRECAO DO PRAZO DE VIGENCIA; SUBSIDIARIAMENTE, CASO SE ENTENDA NAO SER O CASO DE NULIDADE, A CORRECAO DO ATO ADMINISTRATIVO PARA ADEQUACAO DA VIGENCIA DAS PATENTES. AUTOR: INSTITUTO NACIONAL DA PROPRIEDADE INDUSTRIAL - INPI REU:BOEHRINGER INGELHEIM CORPORATION, BOEHRINGER INGELHEIM KG, BOEHRINGER INGELHEIM VETMEDICA, INC.

B16C Correction of notification of the grant [chapter 16.3 patent gazette]
B21A Patent or certificate of addition expired [chapter 21.1 patent gazette]

Free format text: PATENTE EXTINTA EM 27/07/2020.