JPS5786292A - Preparation of n-benzoyl-l-alanine acylase - Google Patents

Preparation of n-benzoyl-l-alanine acylase

Info

Publication number
JPS5786292A
JPS5786292A JP55161907A JP16190780A JPS5786292A JP S5786292 A JPS5786292 A JP S5786292A JP 55161907 A JP55161907 A JP 55161907A JP 16190780 A JP16190780 A JP 16190780A JP S5786292 A JPS5786292 A JP S5786292A
Authority
JP
Japan
Prior art keywords
alanine
benzoyl
acylase
preparation
culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP55161907A
Other languages
Japanese (ja)
Other versions
JPS5941717B2 (en
Inventor
Takahiro Harada
Eiji Miyagawa
Tsutomu Yoshida
Yasuhito Kido
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujirebio Inc
Original Assignee
Fujirebio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujirebio Inc filed Critical Fujirebio Inc
Priority to JP55161907A priority Critical patent/JPS5941717B2/en
Publication of JPS5786292A publication Critical patent/JPS5786292A/en
Publication of JPS5941717B2 publication Critical patent/JPS5941717B2/en
Expired legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To collect N-benzoyl-L-alanine acylase from a culture, by cultivating a microorganism, belonging to the genus Corynebacterium, and capable of producing the N-benzoyl-L-alanine acylase.
CONSTITUTION: Corynebacterium equi H-7 (FERM-P No. 5765) is cultivated in a culture medium containing a yeast extract, meat extract, etc. as a nitrogen source and an inorganic salt and N-benzoyl-L-alanine or hippuric acid as a carbon source at about 30°C and a pH of 5W8 for 20W48hr with aeration. Then N-benzoyl-L-alanine acylase accumulated in the microbial cell is collected. The resultant enzyme is a novel enzyme, capable of hydrolyzing the N-benzoyl-L- alanine to form L-alanine and benzoic acid, and having a molecular weight of about 320,000.
COPYRIGHT: (C)1982,JPO&Japio
JP55161907A 1980-11-19 1980-11-19 Method for producing N-benzoyl-L-alanine acylase Expired JPS5941717B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55161907A JPS5941717B2 (en) 1980-11-19 1980-11-19 Method for producing N-benzoyl-L-alanine acylase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55161907A JPS5941717B2 (en) 1980-11-19 1980-11-19 Method for producing N-benzoyl-L-alanine acylase

Publications (2)

Publication Number Publication Date
JPS5786292A true JPS5786292A (en) 1982-05-29
JPS5941717B2 JPS5941717B2 (en) 1984-10-09

Family

ID=15744280

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55161907A Expired JPS5941717B2 (en) 1980-11-19 1980-11-19 Method for producing N-benzoyl-L-alanine acylase

Country Status (1)

Country Link
JP (1) JPS5941717B2 (en)

Also Published As

Publication number Publication date
JPS5941717B2 (en) 1984-10-09

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