JPS5678589A - Preparation of enzyme useful for cultivation of animal tissue cell - Google Patents
Preparation of enzyme useful for cultivation of animal tissue cellInfo
- Publication number
- JPS5678589A JPS5678589A JP6964880A JP6964880A JPS5678589A JP S5678589 A JPS5678589 A JP S5678589A JP 6964880 A JP6964880 A JP 6964880A JP 6964880 A JP6964880 A JP 6964880A JP S5678589 A JPS5678589 A JP S5678589A
- Authority
- JP
- Japan
- Prior art keywords
- cultivation
- animal tissue
- tissue cell
- enzyme
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: To prepare an enzyme useful for the cultivation of an animal tissue cell, by cultivating a microorganism belonging to the genus Bacillus.
CONSTITUTION: A microorganism, e.g. Bacillus polymyxa (FERM-P No.412), belonging to the genus Bacillus is cultivated in a liquid culture medium containing a carbon source and a nitrogen source at 35W37°C and pH 5W8 for 2W7 days to produce an enzyme which is useful for the cultivation of an animal tissue cell and shows an action as a neutral protease and a mild proteolytic action on casein in the culture medium. The resultant enzyme is then collected.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6964880A JPS5678589A (en) | 1980-05-27 | 1980-05-27 | Preparation of enzyme useful for cultivation of animal tissue cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6964880A JPS5678589A (en) | 1980-05-27 | 1980-05-27 | Preparation of enzyme useful for cultivation of animal tissue cell |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6112773A Division JPS5651747B2 (en) | 1973-05-31 | 1973-05-31 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5678589A true JPS5678589A (en) | 1981-06-27 |
| JPS5714836B2 JPS5714836B2 (en) | 1982-03-26 |
Family
ID=13408867
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6964880A Granted JPS5678589A (en) | 1980-05-27 | 1980-05-27 | Preparation of enzyme useful for cultivation of animal tissue cell |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5678589A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005026333A1 (en) * | 2003-09-09 | 2005-03-24 | Japan Science And Technology Agency | Technique for suspension culture of adhesive cells using protease and method of producing virus with the use of the cells as host |
| CN102612650A (en) * | 2009-11-18 | 2012-07-25 | 合同酒精株式会社 | Electrophoretic analysis method |
-
1980
- 1980-05-27 JP JP6964880A patent/JPS5678589A/en active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005026333A1 (en) * | 2003-09-09 | 2005-03-24 | Japan Science And Technology Agency | Technique for suspension culture of adhesive cells using protease and method of producing virus with the use of the cells as host |
| CN102612650A (en) * | 2009-11-18 | 2012-07-25 | 合同酒精株式会社 | Electrophoretic analysis method |
| US8951397B2 (en) | 2009-11-18 | 2015-02-10 | Godo Shusei Co., Ltd. | Electrophoretic analysis method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5714836B2 (en) | 1982-03-26 |
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