BR9712761B1 - mÉtodo para a produÇço de 1,3- propanodiol e microorganismo hospedeiro transformado. - Google Patents
mÉtodo para a produÇço de 1,3- propanodiol e microorganismo hospedeiro transformado. Download PDFInfo
- Publication number
- BR9712761B1 BR9712761B1 BRPI9712761-2A BR9712761A BR9712761B1 BR 9712761 B1 BR9712761 B1 BR 9712761B1 BR 9712761 A BR9712761 A BR 9712761A BR 9712761 B1 BR9712761 B1 BR 9712761B1
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- Prior art keywords
- glycerol
- propanediol
- seq
- genes
- enzyme
- Prior art date
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- 235000011009 potassium phosphates Nutrition 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
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- LGUKXHPIRLIAGC-UHFFFAOYSA-N propane-1,3-diol;propane-1,2,3-triol Chemical compound OCCCO.OCC(O)CO LGUKXHPIRLIAGC-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/82—Proteins from microorganisms
- Y10S530/823—Lower fungi, e.g. mold
- Y10S530/824—Yeasts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3060196P | 1996-11-13 | 1996-11-13 | |
| US60/030,601 | 1996-11-13 | ||
| PCT/US1997/020292 WO1998021339A1 (en) | 1996-11-13 | 1997-11-10 | Method for the production of 1,3-propanediol by recombinant organisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR9712761A BR9712761A (pt) | 1999-10-26 |
| BR9712761B1 true BR9712761B1 (pt) | 2010-02-23 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI9712761-2A BR9712761B1 (pt) | 1996-11-13 | 1997-11-10 | mÉtodo para a produÇço de 1,3- propanodiol e microorganismo hospedeiro transformado. |
| BRPI9714313-8A BR9714313B1 (pt) | 1996-11-13 | 1997-11-13 | Método para a produção de recombinantes de 1,3-propanodiol |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI9714313-8A BR9714313B1 (pt) | 1996-11-13 | 1997-11-13 | Método para a produção de recombinantes de 1,3-propanodiol |
Country Status (18)
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|---|---|
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| EP (2) | EP0942989B1 (enExample) |
| JP (2) | JP4327909B2 (enExample) |
| KR (1) | KR100509310B1 (enExample) |
| CN (2) | CN1236063C (enExample) |
| AT (2) | ATE452979T1 (enExample) |
| AU (2) | AU733534B2 (enExample) |
| BR (2) | BR9712761B1 (enExample) |
| CA (2) | CA2269088C (enExample) |
| DE (2) | DE69739715D1 (enExample) |
| DK (1) | DK0946732T3 (enExample) |
| ES (1) | ES2336858T3 (enExample) |
| ID (1) | ID21487A (enExample) |
| IL (2) | IL129722A0 (enExample) |
| MY (1) | MY131754A (enExample) |
| NZ (1) | NZ335392A (enExample) |
| WO (2) | WO1998021339A1 (enExample) |
| ZA (1) | ZA9710194B (enExample) |
Families Citing this family (171)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5686276A (en) | 1995-05-12 | 1997-11-11 | E. I. Du Pont De Nemours And Company | Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism |
| US6428767B1 (en) | 1995-05-12 | 2002-08-06 | E. I. Du Pont De Nemours And Company | Method for identifying the source of carbon in 1,3-propanediol |
| CA2267198C (en) | 1996-11-13 | 2008-06-17 | E.I. Du Pont De Nemours And Company | Method for the production of glycerol by recombinant organisms |
| ID21487A (id) * | 1996-11-13 | 1999-06-17 | Du Pont | Metoda pembuatan 1,3 - propandiol dengan organisme rekombinan |
| CA2311601C (en) | 1997-12-02 | 2008-07-08 | E.I. Du Pont De Nemours And Company | Method for the production of glycerol by recombinant organisms |
| GB9806762D0 (en) * | 1998-03-31 | 1998-05-27 | Neutec Pharma Plc | Treatment and diagnosis of staphylococcal infections |
| US7074608B1 (en) | 1998-05-12 | 2006-07-11 | E. I. Du Pont De Nemours And Company | Method for the production of 1,3-propanediol by recombinant organisms comprising genes for coenzyme B12 synthesis |
| US6432686B1 (en) | 1998-05-12 | 2002-08-13 | E. I. Du Pont De Nemours And Company | Method for the production of 1,3-propanediol by recombinant organisms comprising genes for vitamin B12 transport |
| EP1105514B1 (en) | 1998-08-04 | 2008-02-06 | Metabolix, Inc. | Polyhydroxyalkanoate production from polyols |
| US6468773B1 (en) * | 1999-05-19 | 2002-10-22 | Genencor International, Inc. | Mutant 1,3-propandiol dehydrogenase |
| FR2796081B1 (fr) | 1999-07-09 | 2003-09-26 | Agronomique Inst Nat Rech | Procede de preparation du 1,3-propanediol par un micro-organisme recombinant en l'absence de coenzyme b12 ou de l'un de ses precurseurs |
| EP1586647A1 (en) * | 1999-08-18 | 2005-10-19 | E.I. du Pont de Nemours and Company | Process for the biological production of 1,3-propanediol with high titer |
| PT1204755E (pt) | 1999-08-18 | 2006-11-30 | Du Pont | Processo para a produção biológica de 1, 3-propanodiol |
| ATE338139T1 (de) * | 1999-08-30 | 2006-09-15 | Wisconsin Alumni Res Found | Herstellung von 3-hydroxypropionsäure in rekombinanten organismen |
| US6803218B1 (en) | 1999-09-24 | 2004-10-12 | Genencor Intl., Inc. | Enzymes which dehydrate glycerol |
| FR2800751B1 (fr) | 1999-11-09 | 2003-08-29 | Roquette Freres | Procede de production de 1,3 propanediol par voie fermentaire |
| FR2801058B1 (fr) | 1999-11-16 | 2002-01-18 | Roquette Freres | Procede de purification du 1,3-propanediol a partir d'un milieu de fermentation |
| EP1211234A3 (en) | 2000-11-29 | 2003-11-26 | Samsung Electronics Co., Ltd. | Process for preparing 1,3-alkandiols from 3-hydroxyesters |
| US6617478B2 (en) | 2000-11-29 | 2003-09-09 | Samsung Electronics Co., Ltd. | Process for preparing a 1,3-alkandiol from 3-hydroxyester |
| US7309597B2 (en) * | 2002-01-18 | 2007-12-18 | Cargill, Incorporated | Alanine 2,3-Aminomutase |
| EP1499709B1 (en) | 2002-04-22 | 2012-01-04 | E.I. Du Pont De Nemours And Company | Promoter and plasmid system for genetic engineering |
| BR0311449A (pt) * | 2002-05-08 | 2005-03-15 | Basf Plant Science Gmbh | Processo para aumentar o teor total de óleo em um organismo vegetal ou um tecido, órgão, parte, célula ou material de propagação do mesmo, cassete de expressão transgênica, vetor transgênico, organismo vegetal transgênico, ou tecido, órgão, parte, célula ou material de propagação do mesmo, e, uso dos mesmos |
| US7972832B2 (en) | 2002-08-16 | 2011-07-05 | Danisco Us Inc. | Variant Hypocrea jecorina CBH1 cellulases |
| EP1546304B1 (en) | 2002-10-04 | 2013-05-08 | Danisco US Inc. | Improved production of bacterial strains |
| AU2003287028B2 (en) | 2002-10-04 | 2008-09-04 | E.I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high yield |
| AU2003287625A1 (en) * | 2002-11-06 | 2004-06-03 | University Of Florida | Materials and methods for the efficient production of acetate and other products |
| BR0316897A (pt) | 2002-12-16 | 2005-11-08 | Du Pont | Método de seleção para desidratases dependentes de b12, sequências de ácido nucléico, desidratase mutante dependente de b12, método para a criação de mutantes de desidratase dependente de b12 e métodos para a identificação de desidratase dependente de b12 |
| MXPA05009553A (es) | 2003-03-10 | 2006-03-17 | Broin And Associates Inc | Metodo para producir etanol utilizando almidon como materia prima. |
| US20050233030A1 (en) * | 2004-03-10 | 2005-10-20 | Broin And Associates, Inc. | Methods and systems for producing ethanol using raw starch and fractionation |
| WO2004085611A2 (en) * | 2003-03-21 | 2004-10-07 | The Research Foundation Of State University Of New York | Model for mutually exclusive domain folding molecular switch |
| WO2005001065A2 (en) | 2003-04-01 | 2005-01-06 | Genencor International, Inc. | Variant humicola grisea cbh1.1 |
| US7056439B2 (en) * | 2003-05-06 | 2006-06-06 | Tate & Lyle Ingredidents Americas, Inc. | Process for producing 1, 3-propanediol |
| US7919658B2 (en) | 2003-05-06 | 2011-04-05 | E. I. Du Pont De Nemours And Company | Purification of biologically-produced 1,3-propanediol |
| AU2004293789B2 (en) | 2003-11-21 | 2009-07-23 | Genencor International, Inc. | Expression of granular starch hydrolyzing enzymes in trichoderma and process for producing glucose from granular starch substrates |
| US20050239181A1 (en) * | 2004-03-10 | 2005-10-27 | Broin And Associates, Inc. | Continuous process for producing ethanol using raw starch |
| WO2005093060A1 (ja) * | 2004-03-26 | 2005-10-06 | Nippon Shokubai Co., Ltd. | １，３−プロパンジオール及び／又は３−ヒドロキシプロピオン酸を製造する方法 |
| EP1786831A4 (en) * | 2004-07-30 | 2008-01-23 | Cargill Inc | Alanine 2,3-aminomutases |
| EP1838849B1 (en) | 2004-12-30 | 2015-08-26 | Danisco US Inc. | Variant hypocrea jecorina cbh2 cellulases |
| US20070029252A1 (en) * | 2005-04-12 | 2007-02-08 | Dunson James B Jr | System and process for biomass treatment |
| US7781191B2 (en) * | 2005-04-12 | 2010-08-24 | E. I. Du Pont De Nemours And Company | Treatment of biomass to obtain a target chemical |
| BRPI0612944B1 (pt) * | 2005-04-12 | 2017-11-28 | E.I.Du Pont De Nemours And Company | Method of production of target substance |
| WO2006119386A2 (en) * | 2005-05-02 | 2006-11-09 | Broin And Associates, Inc. | Methods and systems for producing ethanol using raw starch and fractionation |
| US7524660B2 (en) | 2005-05-05 | 2009-04-28 | E.I. Du Pont De Nemours And Company | Utilization of fructose in microbial production strains |
| CN1327001C (zh) * | 2005-06-03 | 2007-07-18 | 清华大学 | 利用生物柴油副产物甘油生产1,3－丙二醇的方法 |
| BRPI0611855A2 (pt) * | 2005-06-16 | 2010-10-05 | Wabash Technologies Inc | conjunto de pedal com um mecanismo de histerese |
| CN100427605C (zh) * | 2005-06-17 | 2008-10-22 | 清华大学 | 一种由粗淀粉原料生产1,3-丙二醇和2,3-丁二醇的方法 |
| DE102005048818A1 (de) | 2005-10-10 | 2007-04-12 | Degussa Ag | Mikrobiologische Herstellung von 3-Hydroxypropionsäure |
| US7919289B2 (en) | 2005-10-10 | 2011-04-05 | Poet Research, Inc. | Methods and systems for producing ethanol using raw starch and selecting plant material |
| EP1948814B1 (en) | 2005-10-26 | 2018-11-21 | Butamax (TM) Advanced Biofuels LLC | Fermentive production of four carbon alcohols |
| US8273558B2 (en) | 2005-10-26 | 2012-09-25 | Butamax(Tm) Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| US9303225B2 (en) | 2005-10-26 | 2016-04-05 | Butamax Advanced Biofuels Llc | Method for the production of isobutanol by recombinant yeast |
| CN100392071C (zh) * | 2005-12-05 | 2008-06-04 | 中国农业大学 | 产乳酸途径缺失的工程菌及其构建方法与应用 |
| CN100463968C (zh) * | 2006-01-27 | 2009-02-25 | 华侨大学 | 一种由生物催化转化甘油制备1,3-丙二醇和二羟基丙酮的方法 |
| EA015438B1 (ru) * | 2006-02-06 | 2011-08-30 | ДСМ АйПи АССЕТС Б.В. | Новые оксидоредуктазы и их применение |
| US20070275139A1 (en) * | 2006-02-10 | 2007-11-29 | Melissa Joerger | Food compositions comprising renewably-based, biodegradable1,3-propanediol |
| CN105175257A (zh) * | 2006-02-10 | 2015-12-23 | 杜邦塔特和莱尔生物产品有限责任公司 | 包括生物学基1,3-丙二醇的单酯和二酯的组合物 |
| CN1935991B (zh) * | 2006-06-21 | 2010-05-12 | 南宁中诺生物工程有限责任公司 | 产气荚膜梭菌甘油脱水酶基因及其1,3-丙二醇的生产方法 |
| JP5130811B2 (ja) * | 2006-07-19 | 2013-01-30 | 東レ株式会社 | 連続発酵による１，３−プロパンジオールの製造方法 |
| DE102006039203B4 (de) * | 2006-08-22 | 2014-06-18 | Evonik Degussa Gmbh | Verfahren zur Herstellung von durch Kristallisation gereinigter Acrylsäure aus Hydroxypropionsäure sowie Vorrichtung dazu |
| JP2010508013A (ja) * | 2006-10-31 | 2010-03-18 | メタボリック エクスプローラー | グリセロールから１，３−プロパンジオールを高収量で生物学的に製造する方法 |
| WO2008052596A1 (en) * | 2006-10-31 | 2008-05-08 | Metabolic Explorer | Process for the biological production of n-butanol with high yield |
| WO2008061187A1 (en) * | 2006-11-15 | 2008-05-22 | Dupont Tate & Lyle Bio Products Company, Llc | Preservative compositions comprising renewably-based, biodegradable 1,3-propanediol |
| DE102007015583A1 (de) | 2007-03-29 | 2008-10-02 | Albert-Ludwigs-Universität Freiburg | Ein Enzym zur Herstellung von Methylmalonyl-Coenzym A oder Ethylmalonyl-Coenzym A sowie dessen Verwendung |
| CN101130782B (zh) * | 2007-07-23 | 2011-01-26 | 江南大学 | 以葡萄糖为底物产1,3-丙二醇重组酿酒酵母的构建方法 |
| US7819976B2 (en) * | 2007-08-22 | 2010-10-26 | E. I. Du Pont De Nemours And Company | Biomass treatment method |
| US8445236B2 (en) * | 2007-08-22 | 2013-05-21 | Alliance For Sustainable Energy Llc | Biomass pretreatment |
| US7807419B2 (en) * | 2007-08-22 | 2010-10-05 | E. I. Du Pont De Nemours And Company | Process for concentrated biomass saccharification |
| US20090053800A1 (en) * | 2007-08-22 | 2009-02-26 | Julie Friend | Biomass Treatment Apparatus |
| DE102007041862A1 (de) | 2007-09-03 | 2009-03-19 | Evonik Degussa Gmbh | Mikrobiologische Herstellung von Isoprenoiden |
| EP2594645A3 (en) | 2007-09-21 | 2013-11-06 | BASF Plant Science GmbH | Plants with increased yield |
| CN101205541B (zh) * | 2007-10-18 | 2010-12-08 | 北京化工大学 | 重组表达载体和用其转化的宿主细胞发酵甘油高产1,3-丙二醇的方法 |
| US8048624B1 (en) | 2007-12-04 | 2011-11-01 | Opx Biotechnologies, Inc. | Compositions and methods for 3-hydroxypropionate bio-production from biomass |
| DE102007060705A1 (de) | 2007-12-17 | 2009-06-18 | Evonik Degussa Gmbh | ω-Aminocarbonsäuren oder ihre Lactame, herstellende, rekombinante Zellen |
| MX2010006679A (es) | 2007-12-17 | 2010-11-30 | Univ Amsterdam | Reduccion de co2 inducida con luz para compuestos orgánicos que sirven como combustibles o como semiproductos industriales por un autotrofo que contiene un casete genico fermentador. |
| EP2628796A1 (en) | 2008-06-06 | 2013-08-21 | Danisco US Inc. | Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials |
| DE102008002715A1 (de) | 2008-06-27 | 2009-12-31 | Evonik Röhm Gmbh | 2-Hydroxyisobuttersäure produzierende rekombinante Zelle |
| DE102008041299A1 (de) | 2008-08-18 | 2010-02-25 | Evonik Degussa Gmbh | Neuartiges, universell einsetzbares addiction-System |
| WO2010031083A2 (en) * | 2008-09-15 | 2010-03-18 | Opx Biotechnologies, Inc. | Methods, systems and compositions related to reduction of conversions of microbially produced 3-hydroxypropionic acid (3-hp) to aldehyde metabolites |
| WO2010066411A2 (en) | 2008-12-10 | 2010-06-17 | Direvo Industrial Biotechnology Gmbh | Polypeptides having cellobiohydrolase ii activity |
| US20100159521A1 (en) * | 2008-12-19 | 2010-06-24 | E. I. Du Pont De Nemours And Company | Ozone treatment of biomass to enhance enzymatic saccharification |
| US8450094B1 (en) | 2009-03-03 | 2013-05-28 | Poet Research, Inc. | System for management of yeast to facilitate the production of ethanol |
| HUE025623T2 (en) | 2009-03-03 | 2016-04-28 | Poet Res Inc | A method of fermenting biomass for ethanol production |
| US9068206B1 (en) | 2009-03-03 | 2015-06-30 | Poet Research, Inc. | System for treatment of biomass to facilitate the production of ethanol |
| US20100233771A1 (en) * | 2009-03-03 | 2010-09-16 | Mcdonald William F | System for pre-treatment of biomass for the production of ethanol |
| CN104862293A (zh) | 2009-06-03 | 2015-08-26 | 丹尼斯科美国公司 | 具有改善的表达、活性和/或稳定性的纤维素酶变体,及其用途 |
| US8809027B1 (en) | 2009-09-27 | 2014-08-19 | Opx Biotechnologies, Inc. | Genetically modified organisms for increased microbial production of 3-hydroxypropionic acid involving an oxaloacetate alpha-decarboxylase |
| JP2013505727A (ja) | 2009-09-27 | 2013-02-21 | オーピーエックス バイオテクノロジーズ， インコーポレイテッド | ３−ヒドロキシプロピオン酸および他の生成物を生成するための方法 |
| US8852903B2 (en) | 2009-10-23 | 2014-10-07 | E I Du Pont De Nemours And Company | Co-metabolism of fructose and glucose in microbial production strains |
| DE102009046623A1 (de) | 2009-11-11 | 2011-05-12 | Evonik Röhm Gmbh | Verwendung eines zu einem MeaB-Protein homologen Proteins zur Erhöhung der enzymatischen Aktivität einer 3-Hydroxycarbonsäure-CoA-Mutase |
| DE102010014680A1 (de) | 2009-11-18 | 2011-08-18 | Evonik Degussa GmbH, 45128 | Zellen, Nukleinsäuren, Enzyme und deren Verwendung sowie Verfahren zur Herstellung von Sophorolipiden |
| US20110125118A1 (en) * | 2009-11-20 | 2011-05-26 | Opx Biotechnologies, Inc. | Production of an Organic Acid and/or Related Chemicals |
| US20110136190A1 (en) | 2009-12-04 | 2011-06-09 | E. I. Du Pont De Nemours And Company | Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose |
| US8034592B2 (en) | 2009-12-10 | 2011-10-11 | The Curators Of The University Of Missouri | Fossil fuel-free process of lignocellulosic pretreatment with biological hydrogen production |
| CN102781901B (zh) | 2010-02-11 | 2016-08-03 | 梅塔玻利克斯公司 | 用于从经遗传修饰的聚羟基链烷酸酯生物质制备单体组分的方法 |
| KR101144235B1 (ko) * | 2010-02-25 | 2012-05-10 | 한국과학기술연구원 | 바이오에탄올 폐발효액을 이용한 부탄올의 제조방법 |
| WO2011132511A1 (ja) * | 2010-04-19 | 2011-10-27 | サッポロビール株式会社 | 新規微生物並びにそれを用いた水素製造方法、１，３－プロパンジオール製造方法及びバイオディーゼル廃液の処理方法 |
| US8906235B2 (en) | 2010-04-28 | 2014-12-09 | E I Du Pont De Nemours And Company | Process for liquid/solid separation of lignocellulosic biomass hydrolysate fermentation broth |
| US8721794B2 (en) | 2010-04-28 | 2014-05-13 | E I Du Pont De Nemours And Company | Production of high solids syrup from lignocellulosic biomass hydrolysate fermentation broth |
| KR101220499B1 (ko) * | 2010-05-25 | 2013-01-10 | 한국생명공학연구원 | 글리세롤로부터 3-하이드록시프로피온산을 고수율로 제조하는 방법 |
| JP5998130B2 (ja) | 2010-07-12 | 2016-09-28 | ユニヴェルシテイト ヘントＵｎｉｖｅｒｓｉｔｅｉｔ Ｇｅｎｔ | 付加価値バイオ生成物の生成のための代謝操作された生物 |
| DE102010032484A1 (de) | 2010-07-28 | 2012-02-02 | Evonik Goldschmidt Gmbh | Zellen und Verfahren zur Herstellung von Rhamnolipiden |
| US20130224804A1 (en) * | 2010-09-01 | 2013-08-29 | Eric Knight | Expression of steady state metabolic pathways |
| US8129170B1 (en) | 2010-12-06 | 2012-03-06 | E.I. Du Pont De Nemours And Company | Recombinant bacteria having the ability to metabolize sucrose |
| EP3483279A1 (en) * | 2011-04-05 | 2019-05-15 | Lallemand Hungary Liquidity Management LLC | Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors |
| EP2697349A4 (en) | 2011-04-13 | 2015-01-07 | Elc Man Llc | SOFT ANIONIC SURFACTANTS USEFUL IN BODY HYGIENE COMPOSITIONS |
| JP5852732B2 (ja) | 2011-04-13 | 2016-02-03 | イーエルシー マネージメント エルエルシー | パーソナルケア組成物用コンディショニング剤 |
| FR2976949B1 (fr) * | 2011-06-21 | 2015-08-28 | Sanofi Sa | Procede de preparation de levures genetiquement transformees capables de produire une molecule d'interet a haut titre |
| US8829206B2 (en) | 2011-06-24 | 2014-09-09 | Eastman Chemical Company | Production of cyclic acetals or ketals using solid acid catalysts |
| US9000229B2 (en) | 2011-06-24 | 2015-04-07 | Eastman Chemical Company | Production of hydroxy ether hydrocarbons by vapor phase hydrogenolysis of cyclic acetals and ketals |
| US8829207B2 (en) | 2011-06-24 | 2014-09-09 | Eastman Chemical Company | Production of cyclic acetals by reactive distillation |
| US9388105B2 (en) | 2011-06-24 | 2016-07-12 | Eastman Chemical Company | Production of hydroxy ether hydrocarbons by liquid phase hydrogenolysis of cyclic acetals or cyclic ketals |
| US9056313B2 (en) | 2011-06-24 | 2015-06-16 | Eastman Chemical Company | Catalysts for the production of hydroxy ether hydrocarbons by vapor phase hydrogenolysis of cyclic acetals and ketals |
| US8969598B2 (en) | 2011-06-24 | 2015-03-03 | Eastman Chemical Company | Production of cyclic acetals or ketals using liquid-phase acid catalysts |
| US8785697B2 (en) | 2011-06-24 | 2014-07-22 | Eastman Chemical Company | Nickel modified catalyst for the production of hydroxy ether hydrocarbons by vapor phase hydrogenolysis of cyclic acetals and ketals |
| EP2540834A1 (en) | 2011-06-29 | 2013-01-02 | Metabolic Explorer | Method for the preparation of 1,3-propanediol |
| JP2013013353A (ja) * | 2011-07-01 | 2013-01-24 | Daicel Corp | １，３−プロパンジオールの製造方法 |
| JP2013070675A (ja) * | 2011-09-28 | 2013-04-22 | Daicel Corp | アルキルジオールを産生する組換え菌の培養方法 |
| DE102012201360A1 (de) | 2012-01-31 | 2013-08-01 | Evonik Industries Ag | Zellen und Verfahren zur Herstellung von Rhamnolipiden |
| US9017961B2 (en) | 2012-03-05 | 2015-04-28 | E.I. Du Pont De Nemours And Company | Recombinant bacteria comprising novel sucrose transporters |
| US8686114B2 (en) | 2012-03-05 | 2014-04-01 | E I Du Pont De Nemours And Company | Variant sucrose transporter polypeptides |
| CN102634543A (zh) * | 2012-05-04 | 2012-08-15 | 杭玉玲 | 微生物发酵生产1,3-丙二醇的方法 |
| BR112014030203B1 (pt) | 2012-06-08 | 2021-10-13 | Cj Cheiljedang Corporation | Processo para produção de produtos de ácido acrílico de base biológica |
| EP2882843A4 (en) | 2012-08-10 | 2016-04-06 | Opx Biotechnologies Inc | MICRO-ORGANISMS AND METHOD FOR THE PRODUCTION OF FATTY ACIDS AND PRODUCTS DERIVED FROM FATTY ACIDS |
| KR101437040B1 (ko) * | 2012-08-17 | 2014-09-02 | 삼성전자 주식회사 | 삼투압 보호제 첨가에 의한 1,3-프로판디올 생산방법 및 생산용 배지 |
| CA2882276C (en) * | 2012-08-28 | 2016-11-01 | Lanzatech New Zealand Limited | Recombinant microorganisms expressing a stereospecific diol dehydratase enzyme |
| EP3290515A1 (en) | 2012-12-12 | 2018-03-07 | Danisco US Inc. | Variants of cellobiohydrolases |
| US20140178954A1 (en) | 2012-12-20 | 2014-06-26 | E I Du Pont De Nemours And Company | Expression of xylose isomerase activity in yeast |
| US20150376152A1 (en) | 2013-02-13 | 2015-12-31 | Metabolix, Inc. | Process for Ultra Pure Chemical Production from Biobased Raw Starting Materials |
| US9187743B2 (en) | 2013-03-11 | 2015-11-17 | E I Du Pont De Nemours And Company | Bacterial xylose isomerases active in yeast cells |
| US8669076B1 (en) | 2013-03-11 | 2014-03-11 | E I Du Pont De Nemours And Company | Cow rumen xylose isomerases active in yeast cells |
| US20140273105A1 (en) | 2013-03-12 | 2014-09-18 | E I Du Pont De Nemours And Company | Gradient pretreatment of lignocellulosic biomass |
| US10584860B2 (en) * | 2013-03-14 | 2020-03-10 | Ideal Industries, Llc | Linear light fixture with interchangeable light engine unit |
| ES2735024T3 (es) * | 2013-03-14 | 2019-12-13 | Du Pont | Glicerol-3-fosfato deshidrogenasa para la producción de butanol |
| WO2014160262A1 (en) | 2013-03-14 | 2014-10-02 | Abengoa Bioenergy New Technologies, Llc | Methods for converting cellulosic waste to bioproducts |
| EP2976141A4 (en) | 2013-03-15 | 2016-10-05 | Cargill Inc | FLASHING FOR PRODUCTION CLEANING AND RECOVERY |
| US20150057465A1 (en) | 2013-03-15 | 2015-02-26 | Opx Biotechnologies, Inc. | Control of growth-induction-production phases |
| CN106795483A (zh) | 2013-07-19 | 2017-05-31 | 嘉吉公司 | 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法 |
| US11408013B2 (en) | 2013-07-19 | 2022-08-09 | Cargill, Incorporated | Microorganisms and methods for the production of fatty acids and fatty acid derived products |
| US10035749B2 (en) | 2013-09-03 | 2018-07-31 | Myriant Corporation | Process for manufacturing acrylic acid, acrylonitrile and 1,4-butanediol from 1,3-propanediol |
| US9328360B2 (en) | 2013-09-06 | 2016-05-03 | The Curators Of The University Of Missouri | Conversion of glycerol to 1,3-propanediol under haloalkaline conditions |
| US10227623B2 (en) | 2013-11-24 | 2019-03-12 | E I Du Pont De Nemours And Company | High force and high stress destructuring of cellulosic biomass |
| KR101532736B1 (ko) | 2014-02-24 | 2015-07-09 | 한국생명공학연구원 | 2,3-부탄디올 합성 유전자가 결실된 미생물 변이체를 이용한 1,3-프로판디올의 제조방법 |
| FR3015186A1 (fr) * | 2014-03-11 | 2015-06-26 | Antoine Piccirilli | Utilisation d'une composition renfermant du 1,3-propanediol comme e-liquide |
| EP2949214A1 (en) | 2014-05-26 | 2015-12-02 | Evonik Degussa GmbH | Methods of producing rhamnolipids |
| WO2016007350A1 (en) | 2014-07-09 | 2016-01-14 | Danisco Us Inc. | Preconditioning of lignocellulosic biomass |
| EP2993228B1 (en) | 2014-09-02 | 2019-10-09 | Cargill, Incorporated | Production of fatty acid esters |
| EP3056561A1 (de) | 2015-02-16 | 2016-08-17 | Evonik Degussa GmbH | Mikroorganismen mit reduzierter Enzymaktivität |
| JP2018505690A (ja) | 2015-02-19 | 2018-03-01 | エボニック デグサ ゲーエムベーハーＥｖｏｎｉｋ Ｄｅｇｕｓｓａ ＧｍｂＨ | ラムノ脂質合成 |
| HK1246493A1 (zh) * | 2015-04-20 | 2018-09-07 | Pi-克瑞斯托株式会社 | 有源矩阵阵列装置的制造方法和通过该方法制造的有源矩阵阵列装置 |
| CN104774879B (zh) * | 2015-04-29 | 2017-10-20 | 大连理工大学 | 一种混菌发酵甘油生产1,3‑丙二醇的方法 |
| WO2017017292A1 (es) | 2015-07-29 | 2017-02-02 | Abengoa Bioenergía Nuevas Tecnologías, S. A. | Expresión de enzimas beta-xilosidasas recombinantes |
| US9968531B2 (en) | 2015-08-05 | 2018-05-15 | Dupont Tate & Lyle Bio Products Company, Llc | Deodorants containing 1,3-propanediol |
| EP3192866A1 (en) | 2016-01-15 | 2017-07-19 | CIC nanoGUNE - Asociación Centro de Investigación Cooperativa en Nanociencias | Endocellulases and uses thereof |
| BR112018070645A2 (pt) | 2016-04-08 | 2019-02-05 | Du Pont | células de levedura recombinante, métodos para produzir uma célula de levedura e método para produzir um composto alvo |
| JP7597507B2 (ja) | 2016-10-24 | 2024-12-10 | エボニック オペレーションズ ゲーエムベーハー | 代替グルコーストランスポーターを使用してラムノリピドを製造するための細胞および方法 |
| JP7143292B2 (ja) | 2016-10-24 | 2022-09-28 | エボニック オペレーションズ ゲーエムベーハー | 減少したグルコースデヒドロゲナーゼ活性を有するラムノリピド産生細胞 |
| WO2018144701A2 (en) | 2017-02-02 | 2018-08-09 | Cargill Incorporated | Genetically modified cells that produce c6-c10 fatty acid derivatives |
| EP3502126A1 (en) | 2017-12-19 | 2019-06-26 | CIC nanoGUNE - Asociación Centro de Investigación Cooperativa en Nanociencias | Ancestral cellulases and uses thereof |
| KR102103408B1 (ko) * | 2018-01-16 | 2020-04-23 | 한국과학기술원 | 1,3-프로판디올 생성능을 가지는 변이미생물 및 이를 이용한 1,3-pdo의 제조방법 |
| WO2019152755A1 (en) * | 2018-02-01 | 2019-08-08 | Invista North America S.A.R.L. | Methods and materials for the biosynthesis of beta hydroxy acids and/or derivatives thereof and/or compounds related thereto |
| JP7411557B2 (ja) | 2018-02-09 | 2024-01-11 | エボニック オペレーションズ ゲーエムベーハー | グルコリピドを含有する混合組成物 |
| CN111386345B (zh) * | 2018-10-30 | 2023-05-16 | 绿色地球研究所株式会社 | 1,3-丙二醇的制造方法 |
| CN109370969B (zh) * | 2018-11-12 | 2020-09-04 | 江南大学 | 一种重组克雷伯氏杆菌在制备1，3-丙二醇中的应用 |
| CN109337852B (zh) * | 2018-11-12 | 2020-09-04 | 江南大学 | 一种重组克雷伯氏杆菌在生产1,3-丙二醇中的应用 |
| CN109750011B (zh) * | 2019-02-20 | 2022-05-10 | 中国科学院天津工业生物技术研究所 | 一种甘露糖6-磷酸磷酸酶及甘露糖生物制备方法 |
| KR20210128742A (ko) * | 2020-04-17 | 2021-10-27 | 에스케이이노베이션 주식회사 | 글리세롤 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법 |
| US20230348942A1 (en) * | 2020-11-05 | 2023-11-02 | Lg Chem, Ltd. | Microorganisms having capability of producing 3-hydroxypropionic acid from glucose and uses thereof |
| CN112625994B (zh) * | 2021-01-05 | 2022-04-15 | 清华大学 | 一种重组需钠弧菌及其应用 |
| CN114806981B (zh) * | 2021-01-18 | 2023-05-23 | 中国科学院上海高等研究院 | 克雷伯氏菌工程菌在生产1,3-丙二醇中的应用 |
| CN114107145B (zh) * | 2021-11-04 | 2024-05-07 | 清华大学 | 一种重组微生物及其在生产1,3-丙二醇中的应用 |
| EP4709845A1 (en) | 2023-05-11 | 2026-03-18 | Evonik Operations GmbH | Glucolipid production |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3634764A1 (de) | 1986-10-11 | 1988-04-14 | Mueller Haidmann Roland Dipl I | Aus platten zusammengesetzte decke |
| DE3734764A1 (de) * | 1987-10-14 | 1989-05-03 | Huels Chemische Werke Ag | Verfahren zur herstellung von propandiol-(1,3) |
| DE3878564T2 (de) * | 1988-12-12 | 1993-08-19 | Unilever Nv | Verfahren zur mikrobiologischen herstellung von 1,3-propandiol aus glycerin. |
| US5686276A (en) * | 1995-05-12 | 1997-11-11 | E. I. Du Pont De Nemours And Company | Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism |
| US5633362A (en) * | 1995-05-12 | 1997-05-27 | E. I. Du Pont De Nemours And Company | Production of 1,3-propanediol from glycerol by recombinant bacteria expressing recombinant diol dehydratase |
| US5599689A (en) * | 1995-05-12 | 1997-02-04 | E. I. Du Pont De Nemours And Company | Process for making 1,3-propanediol from carbohydrates using mixed microbial cultures |
| ID21487A (id) * | 1996-11-13 | 1999-06-17 | Du Pont | Metoda pembuatan 1,3 - propandiol dengan organisme rekombinan |
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