BR112015013685A8 - meio de cultura e solução de nutriente isenta de soro para crescimento de células dependentes de ancoragem e método para cultivar células derivadas de tecido do cordão umbilical isoladas - Google Patents
meio de cultura e solução de nutriente isenta de soro para crescimento de células dependentes de ancoragem e método para cultivar células derivadas de tecido do cordão umbilical isoladas Download PDFInfo
- Publication number
- BR112015013685A8 BR112015013685A8 BR112015013685A BR112015013685A BR112015013685A8 BR 112015013685 A8 BR112015013685 A8 BR 112015013685A8 BR 112015013685 A BR112015013685 A BR 112015013685A BR 112015013685 A BR112015013685 A BR 112015013685A BR 112015013685 A8 BR112015013685 A8 BR 112015013685A8
- Authority
- BR
- Brazil
- Prior art keywords
- serum
- anchor
- culture medium
- cell growth
- dependent cell
- Prior art date
Links
- 235000015097 nutrients Nutrition 0.000 title abstract 4
- 230000010261 cell growth Effects 0.000 title abstract 3
- 230000001419 dependent effect Effects 0.000 title abstract 3
- 239000001963 growth medium Substances 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 238000012258 culturing Methods 0.000 title 1
- 210000003954 umbilical cord Anatomy 0.000 title 1
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 abstract 4
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 4
- 102000004877 Insulin Human genes 0.000 abstract 2
- 108090001061 Insulin Proteins 0.000 abstract 2
- 102000004338 Transferrin Human genes 0.000 abstract 2
- 108090000901 Transferrin Proteins 0.000 abstract 2
- 150000001413 amino acids Chemical class 0.000 abstract 2
- 229940125396 insulin Drugs 0.000 abstract 2
- 239000002777 nucleoside Substances 0.000 abstract 2
- 125000003835 nucleoside group Chemical group 0.000 abstract 2
- 150000003839 salts Chemical class 0.000 abstract 2
- HVAKSLUOHARFLM-UHFFFAOYSA-N selenium;sodium Chemical compound [Se][Na] HVAKSLUOHARFLM-UHFFFAOYSA-N 0.000 abstract 2
- 210000002966 serum Anatomy 0.000 abstract 2
- 239000012581 transferrin Substances 0.000 abstract 2
- 235000013343 vitamin Nutrition 0.000 abstract 2
- 239000011782 vitamin Substances 0.000 abstract 2
- 229940088594 vitamin Drugs 0.000 abstract 2
- 229930003231 vitamin Natural products 0.000 abstract 2
- 230000012010 growth Effects 0.000 abstract 1
- 239000004017 serum-free culture medium Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
- C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Hematology (AREA)
- Rheumatology (AREA)
- Pregnancy & Childbirth (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/715,532 US20140170748A1 (en) | 2012-12-14 | 2012-12-14 | Nutrient Enriched Media for hUTC Growth |
| PCT/US2013/074615 WO2014093598A1 (en) | 2012-12-14 | 2013-12-12 | NUTRIENT ENRICHED MEDIA FOR hUTC GROWTH |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112015013685A2 BR112015013685A2 (pt) | 2017-07-11 |
| BR112015013685A8 true BR112015013685A8 (pt) | 2019-11-05 |
Family
ID=49881127
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112015013685A BR112015013685A8 (pt) | 2012-12-14 | 2013-12-12 | meio de cultura e solução de nutriente isenta de soro para crescimento de células dependentes de ancoragem e método para cultivar células derivadas de tecido do cordão umbilical isoladas |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US20140170748A1 (enExample) |
| EP (2) | EP3382007A1 (enExample) |
| JP (2) | JP6404227B2 (enExample) |
| KR (2) | KR102151588B1 (enExample) |
| CN (2) | CN111690602A (enExample) |
| AR (2) | AR093985A1 (enExample) |
| AU (2) | AU2013359265B2 (enExample) |
| BR (1) | BR112015013685A8 (enExample) |
| CA (1) | CA2895079C (enExample) |
| ES (1) | ES2667573T3 (enExample) |
| MX (2) | MX364731B (enExample) |
| PH (1) | PH12015501310B1 (enExample) |
| PL (1) | PL2931875T3 (enExample) |
| RU (2) | RU2684306C2 (enExample) |
| SG (2) | SG10201801924VA (enExample) |
| TW (1) | TWI620818B (enExample) |
| WO (1) | WO2014093598A1 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108471736A (zh) | 2016-01-14 | 2018-08-31 | 德普伊新特斯产品公司 | 用于冷冻保存hutc的组合物和方法 |
| US10894947B1 (en) * | 2016-04-29 | 2021-01-19 | Hope Biosciences, Llc | Method for generating protein rich conditioned medium |
| US11111480B2 (en) | 2016-04-29 | 2021-09-07 | Hope Biosctences, Llc | Culture media for multipotent stem cells |
| CN106434532A (zh) * | 2016-12-22 | 2017-02-22 | 叶宗耀 | 一种培养肝细胞的培养基及其制备方法 |
| CN106957815B (zh) * | 2017-03-16 | 2021-05-07 | 杨涛 | 一种用于人类多潜能干细胞的无血清培养基的配方 |
| CN110494559B (zh) * | 2017-03-28 | 2023-10-31 | 味之素株式会社 | 不分化地维持用培养基添加剂 |
| CN107119017B (zh) * | 2017-05-25 | 2020-05-26 | 山东省医药生物技术研究中心 | 一种骨肉瘤细胞的无血清培养基及其制备方法 |
| JPWO2020067502A1 (ja) * | 2018-09-28 | 2021-08-30 | 味の素株式会社 | 動物細胞の培養用添加物、培養用培地および培養方法 |
| US20220145264A1 (en) * | 2019-04-05 | 2022-05-12 | The University Of Hong Kong | Culture medium for mammalian expanded potential stem cells, composition, and methods thereof |
| JP7650818B2 (ja) | 2019-04-26 | 2025-03-25 | アロジーン セラピューティクス，インコーポレイテッド | 同種ｃａｒ ｔ細胞を製造する方法 |
| EP3983481A1 (en) * | 2019-06-13 | 2022-04-20 | Corning Incorporated | Dissolvable microcarriers for culturing cells and related methods |
| CN114058573B (zh) * | 2020-08-03 | 2024-04-05 | 上海原天生物科技有限公司 | 一种含有生物素的培养基 |
| CN112608892B (zh) * | 2020-12-25 | 2023-11-14 | 深圳博雅感知药业有限公司 | 血小板裂解物用于无血清分离和传代培养脐带间充质干细胞的方法 |
| KR20220104321A (ko) | 2021-01-18 | 2022-07-26 | 주식회사 엑소코바이오 | 엑소좀 및/또는 세포외 소포체의 생성 촉진 내지는 생산성 향상 방법 |
| US11735303B2 (en) | 2021-06-22 | 2023-08-22 | David Haase | Apparatus and method for determining a composition of a replacement therapy treatment |
| JPWO2024253069A1 (enExample) * | 2023-06-06 | 2024-12-12 | ||
| KR20250140345A (ko) * | 2024-03-18 | 2025-09-25 | 주식회사 심플플래닛 | 세포 증식 활성이 우수한 식용 배지 조성물 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
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| ZA776251B (en) * | 1976-11-11 | 1978-07-26 | Massachusetts Inst Technology | Improved cell culture microcarriers |
| AU653927B2 (en) * | 1990-01-22 | 1994-10-20 | United States of America, as represented by the Secretary, U.S. Department of Commerce, The | Co-independent growth medium for maintenance and propagation of cells |
| JP2993614B2 (ja) * | 1990-11-20 | 1999-12-20 | 有限会社野々川商事 | 新規化粧料、グルコサミノグリカン生成促進剤および美白剤 |
| US7556958B1 (en) | 1997-04-08 | 2009-07-07 | The Rockefeller University | Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof |
| US6900056B2 (en) * | 2001-02-15 | 2005-05-31 | Centocor, Inc. | Chemically defined medium for cultured mammalian cells |
| US9969980B2 (en) | 2001-09-21 | 2018-05-15 | Garnet Biotherapeutics | Cell populations which co-express CD49c and CD90 |
| ES2370012T3 (es) * | 2002-01-09 | 2011-12-12 | Alberto Gorrochategui Barrueta | Composición para la creación, regeneración y reparación tisular mediante un implante biológico que porta células enriquecido con concentrado de plaquetas y complementos. |
| US20060223177A1 (en) * | 2003-06-27 | 2006-10-05 | Ethicon Inc. | Postpartum cells derived from umbilical cord tissue, and methods of making and using the same |
| US8518390B2 (en) * | 2003-06-27 | 2013-08-27 | Advanced Technologies And Regenerative Medicine, Llc | Treatment of stroke and other acute neural degenerative disorders via intranasal administration of umbilical cord-derived cells |
| AU2004252571C1 (en) | 2003-06-27 | 2012-03-01 | Ethicon, Incorporated | Postpartum-derived cells for use in treatment of disease of the heart and circulatory system |
| WO2005007863A2 (en) | 2003-07-10 | 2005-01-27 | National Corn Growers Association | Distillers solubles as the primary constituent in protein blocks for livestock |
| JP2007522796A (ja) * | 2003-11-07 | 2007-08-16 | ユニヴァーシティ オブ フロリダ リサーチ ファウンデーション，インク． | 神経前駆細胞の培養と分化 |
| EP1812557A4 (en) * | 2004-10-29 | 2009-11-04 | Centocor Ortho Biotech Inc | COMPOSITIONS OF CHEMICALLY DEFINED MEDIA |
| ES2391034T3 (es) | 2005-12-19 | 2012-11-20 | Ethicon, Inc. | Expansión in vitro de células derivadas postparto en frascos rotatorios |
| GB2454386B (en) * | 2006-07-06 | 2011-07-06 | Es Cell Int Pte Ltd | Method for embryonic stem cell culture on a positively charged support surface |
| WO2008060541A2 (en) * | 2006-11-13 | 2008-05-22 | Ethicon, Incorporated | In vitro expansion of postpartum-derived cells using microcarriers |
| WO2008082525A1 (en) * | 2006-12-19 | 2008-07-10 | National Stem Cell Inc | Umbilical cord stem cell secreted product derived topical compositions and methods of use thereof |
| EP2028268A1 (en) * | 2007-08-20 | 2009-02-25 | Université Libre De Bruxelles | Generation of neuronal cells from pluripotent stem cells |
| CN100482783C (zh) * | 2007-11-27 | 2009-04-29 | 天津百若克医药生物技术有限责任公司 | 一种用于Vero细胞的培养基及其培养方法 |
| US10179900B2 (en) * | 2008-12-19 | 2019-01-15 | DePuy Synthes Products, Inc. | Conditioned media and methods of making a conditioned media |
| WO2011017050A1 (en) * | 2009-07-28 | 2011-02-10 | Corning Incorporated | Synthetic microcarriers for culturing cells |
-
2012
- 2012-12-14 US US13/715,532 patent/US20140170748A1/en not_active Abandoned
-
2013
- 2013-12-12 CA CA2895079A patent/CA2895079C/en active Active
- 2013-12-12 BR BR112015013685A patent/BR112015013685A8/pt active Search and Examination
- 2013-12-12 KR KR1020197003828A patent/KR102151588B1/ko not_active Expired - Fee Related
- 2013-12-12 SG SG10201801924VA patent/SG10201801924VA/en unknown
- 2013-12-12 ES ES13812432.6T patent/ES2667573T3/es active Active
- 2013-12-12 MX MX2015007594A patent/MX364731B/es active IP Right Grant
- 2013-12-12 SG SG11201504547SA patent/SG11201504547SA/en unknown
- 2013-12-12 AU AU2013359265A patent/AU2013359265B2/en not_active Ceased
- 2013-12-12 CN CN202010511518.7A patent/CN111690602A/zh active Pending
- 2013-12-12 KR KR1020157018827A patent/KR101948237B1/ko not_active Expired - Fee Related
- 2013-12-12 EP EP18159408.6A patent/EP3382007A1/en not_active Withdrawn
- 2013-12-12 RU RU2018112452A patent/RU2684306C2/ru active
- 2013-12-12 EP EP13812432.6A patent/EP2931875B1/en not_active Not-in-force
- 2013-12-12 PL PL13812432T patent/PL2931875T3/pl unknown
- 2013-12-12 WO PCT/US2013/074615 patent/WO2014093598A1/en not_active Ceased
- 2013-12-12 JP JP2015547532A patent/JP6404227B2/ja not_active Expired - Fee Related
- 2013-12-12 CN CN201380072759.4A patent/CN105026551B/zh not_active Expired - Fee Related
- 2013-12-12 RU RU2015128276A patent/RU2653449C2/ru active
- 2013-12-13 AR ARP130104702A patent/AR093985A1/es unknown
- 2013-12-13 TW TW102146011A patent/TWI620818B/zh not_active IP Right Cessation
-
2015
- 2015-06-09 PH PH12015501310A patent/PH12015501310B1/en unknown
- 2015-06-12 MX MX2018013469A patent/MX2018013469A/es unknown
-
2018
- 2018-09-04 JP JP2018165126A patent/JP6595061B2/ja not_active Expired - Fee Related
-
2019
- 2019-03-29 AU AU2019202191A patent/AU2019202191B2/en not_active Ceased
-
2020
- 2020-02-10 AR ARP200100348A patent/AR118040A2/es unknown
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