JP6404227B2 - hUTC増殖用栄養強化培地 - Google Patents

hUTC増殖用栄養強化培地 Download PDF

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Publication number
JP6404227B2
JP6404227B2 JP2015547532A JP2015547532A JP6404227B2 JP 6404227 B2 JP6404227 B2 JP 6404227B2 JP 2015547532 A JP2015547532 A JP 2015547532A JP 2015547532 A JP2015547532 A JP 2015547532A JP 6404227 B2 JP6404227 B2 JP 6404227B2
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cells
cell
medium
culture
microcarriers
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JP2016501026A (ja
JP2016501026A5 (enExample
Inventor
バティア・ラビンダー
ホン・エル・エス・クローダイン
オズターク・サデッティン・エス
カマラジュ・ベンカット・エイチ
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DePuy Synthes Products Inc
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DePuy Synthes Products Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0605Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0037Serum-free medium, which may still contain naturally-sourced components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
    • C12N5/0075General culture methods using substrates using microcarriers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0665Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin
    • C12N2500/25Insulin-transferrin; Insulin-transferrin-selenium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/32Amino acids
    • C12N2500/33Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2015547532A 2012-12-14 2013-12-12 hUTC増殖用栄養強化培地 Expired - Fee Related JP6404227B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/715,532 2012-12-14
US13/715,532 US20140170748A1 (en) 2012-12-14 2012-12-14 Nutrient Enriched Media for hUTC Growth
PCT/US2013/074615 WO2014093598A1 (en) 2012-12-14 2013-12-12 NUTRIENT ENRICHED MEDIA FOR hUTC GROWTH

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2018165126A Division JP6595061B2 (ja) 2012-12-14 2018-09-04 単離された臍帯組織由来細胞を培養する方法

Publications (3)

Publication Number Publication Date
JP2016501026A JP2016501026A (ja) 2016-01-18
JP2016501026A5 JP2016501026A5 (enExample) 2017-02-16
JP6404227B2 true JP6404227B2 (ja) 2018-10-10

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Family Applications (2)

Application Number Title Priority Date Filing Date
JP2015547532A Expired - Fee Related JP6404227B2 (ja) 2012-12-14 2013-12-12 hUTC増殖用栄養強化培地
JP2018165126A Expired - Fee Related JP6595061B2 (ja) 2012-12-14 2018-09-04 単離された臍帯組織由来細胞を培養する方法

Family Applications After (1)

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JP2018165126A Expired - Fee Related JP6595061B2 (ja) 2012-12-14 2018-09-04 単離された臍帯組織由来細胞を培養する方法

Country Status (17)

Country Link
US (1) US20140170748A1 (enExample)
EP (2) EP2931875B1 (enExample)
JP (2) JP6404227B2 (enExample)
KR (2) KR102151588B1 (enExample)
CN (2) CN105026551B (enExample)
AR (2) AR093985A1 (enExample)
AU (2) AU2013359265B2 (enExample)
BR (1) BR112015013685A8 (enExample)
CA (1) CA2895079C (enExample)
ES (1) ES2667573T3 (enExample)
MX (2) MX364731B (enExample)
PH (1) PH12015501310B1 (enExample)
PL (1) PL2931875T3 (enExample)
RU (2) RU2684306C2 (enExample)
SG (2) SG11201504547SA (enExample)
TW (1) TWI620818B (enExample)
WO (1) WO2014093598A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025198277A1 (ko) * 2024-03-18 2025-09-25 주식회사 심플플래닛 세포 증식 활성이 우수한 식용 배지 조성물

Families Citing this family (16)

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US10791730B2 (en) * 2016-01-14 2020-10-06 DePuy Synthes Products, Inc. Composition and methods for cryopreservation of hUTC
US10894947B1 (en) * 2016-04-29 2021-01-19 Hope Biosciences, Llc Method for generating protein rich conditioned medium
US11111480B2 (en) 2016-04-29 2021-09-07 Hope Biosctences, Llc Culture media for multipotent stem cells
CN106434532A (zh) * 2016-12-22 2017-02-22 叶宗耀 一种培养肝细胞的培养基及其制备方法
CN106957815B (zh) * 2017-03-16 2021-05-07 杨涛 一种用于人类多潜能干细胞的无血清培养基的配方
JP7181534B2 (ja) * 2017-03-28 2022-12-01 味の素株式会社 未分化維持培地添加剤
CN107119017B (zh) * 2017-05-25 2020-05-26 山东省医药生物技术研究中心 一种骨肉瘤细胞的无血清培养基及其制备方法
KR20210068488A (ko) * 2018-09-28 2021-06-09 아지노모토 가부시키가이샤 동물 세포의 배양용 첨가물, 배양용 배지 및 배양 방법
EP3947642A4 (en) * 2019-04-05 2022-12-14 The University of Hong Kong CULTURE MEDIUM FOR MAMMALIAN PLURIPOTENTE STEM CELLS, COMPOSITION AND METHODS
WO2020219812A1 (en) 2019-04-26 2020-10-29 Allogene Therapeutics, Inc. Methods of manufacturing allogeneic car t cells
EP3983481A1 (en) * 2019-06-13 2022-04-20 Corning Incorporated Dissolvable microcarriers for culturing cells and related methods
CN114058573B (zh) * 2020-08-03 2024-04-05 上海原天生物科技有限公司 一种含有生物素的培养基
CN112608892B (zh) * 2020-12-25 2023-11-14 深圳博雅感知药业有限公司 血小板裂解物用于无血清分离和传代培养脐带间充质干细胞的方法
KR20220104321A (ko) 2021-01-18 2022-07-26 주식회사 엑소코바이오 엑소좀 및/또는 세포외 소포체의 생성 촉진 내지는 생산성 향상 방법
US11735303B2 (en) 2021-06-22 2023-08-22 David Haase Apparatus and method for determining a composition of a replacement therapy treatment
WO2024253069A1 (ja) * 2023-06-06 2024-12-12 東ソー株式会社 温度応答性マイクロキャリア

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JP2993614B2 (ja) * 1990-11-20 1999-12-20 有限会社野々川商事 新規化粧料、グルコサミノグリカン生成促進剤および美白剤
US7556958B1 (en) 1997-04-08 2009-07-07 The Rockefeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025198277A1 (ko) * 2024-03-18 2025-09-25 주식회사 심플플래닛 세포 증식 활성이 우수한 식용 배지 조성물

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AU2013359265B2 (en) 2019-01-31
AR118040A2 (es) 2021-09-15
HK1215050A1 (en) 2016-08-12
CA2895079A1 (en) 2014-06-19
JP2018183195A (ja) 2018-11-22
ES2667573T3 (es) 2018-05-11
US20140170748A1 (en) 2014-06-19
CN111690602A (zh) 2020-09-22
BR112015013685A8 (pt) 2019-11-05
EP2931875B1 (en) 2018-03-21
MX364731B (es) 2019-05-06
TW201439321A (zh) 2014-10-16
SG10201801924VA (en) 2018-05-30
KR101948237B1 (ko) 2019-02-14
SG11201504547SA (en) 2015-07-30
EP3382007A1 (en) 2018-10-03
CN105026551B (zh) 2020-07-03
JP2016501026A (ja) 2016-01-18
PH12015501310A1 (en) 2015-09-02
PL2931875T3 (pl) 2018-08-31
TWI620818B (zh) 2018-04-11
AU2013359265A1 (en) 2015-07-16
PH12015501310B1 (en) 2015-09-02
RU2018112452A (ru) 2019-03-01
WO2014093598A1 (en) 2014-06-19
MX2018013469A (es) 2020-11-12
RU2684306C2 (ru) 2019-04-05
KR20190016612A (ko) 2019-02-18
RU2653449C2 (ru) 2018-05-08
AU2019202191B2 (en) 2021-01-21
RU2015128276A (ru) 2017-01-23
BR112015013685A2 (pt) 2017-07-11
MX2015007594A (es) 2016-03-15
EP2931875A1 (en) 2015-10-21
CN105026551A (zh) 2015-11-04
JP6595061B2 (ja) 2019-10-23
KR102151588B1 (ko) 2020-09-04
RU2018112452A3 (enExample) 2019-03-01
KR20150094760A (ko) 2015-08-19
AR093985A1 (es) 2015-07-01
CA2895079C (en) 2021-06-29
AU2019202191A1 (en) 2019-04-18

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