CA2895079C - Nutrient enriched media for hutc growth - Google Patents

Nutrient enriched media for hutc growth Download PDF

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Publication number
CA2895079C
CA2895079C CA2895079A CA2895079A CA2895079C CA 2895079 C CA2895079 C CA 2895079C CA 2895079 A CA2895079 A CA 2895079A CA 2895079 A CA2895079 A CA 2895079A CA 2895079 C CA2895079 C CA 2895079C
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Prior art keywords
cells
cell
microcarriers
medium
serum
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CA2895079A
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English (en)
French (fr)
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CA2895079A1 (en
Inventor
Ravinder Bhatia
L.S. Klaudyne Hong
Sadettin S. OZTURK
Venkat H. KAMARAJU
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DePuy Synthes Products Inc
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DePuy Synthes Products Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0037Serum-free medium, which may still contain naturally-sourced components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0605Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
    • C12N5/0075General culture methods using substrates using microcarriers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0665Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin
    • C12N2500/25Insulin-transferrin; Insulin-transferrin-selenium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/32Amino acids
    • C12N2500/33Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2895079A 2012-12-14 2013-12-12 Nutrient enriched media for hutc growth Active CA2895079C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/715,532 US20140170748A1 (en) 2012-12-14 2012-12-14 Nutrient Enriched Media for hUTC Growth
US13/715,532 2012-12-14
PCT/US2013/074615 WO2014093598A1 (en) 2012-12-14 2013-12-12 NUTRIENT ENRICHED MEDIA FOR hUTC GROWTH

Publications (2)

Publication Number Publication Date
CA2895079A1 CA2895079A1 (en) 2014-06-19
CA2895079C true CA2895079C (en) 2021-06-29

Family

ID=49881127

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2895079A Active CA2895079C (en) 2012-12-14 2013-12-12 Nutrient enriched media for hutc growth

Country Status (17)

Country Link
US (1) US20140170748A1 (enExample)
EP (2) EP3382007A1 (enExample)
JP (2) JP6404227B2 (enExample)
KR (2) KR102151588B1 (enExample)
CN (2) CN111690602A (enExample)
AR (2) AR093985A1 (enExample)
AU (2) AU2013359265B2 (enExample)
BR (1) BR112015013685A8 (enExample)
CA (1) CA2895079C (enExample)
ES (1) ES2667573T3 (enExample)
MX (2) MX364731B (enExample)
PH (1) PH12015501310B1 (enExample)
PL (1) PL2931875T3 (enExample)
RU (2) RU2684306C2 (enExample)
SG (2) SG10201801924VA (enExample)
TW (1) TWI620818B (enExample)
WO (1) WO2014093598A1 (enExample)

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CN108471736A (zh) 2016-01-14 2018-08-31 德普伊新特斯产品公司 用于冷冻保存hutc的组合物和方法
US10894947B1 (en) * 2016-04-29 2021-01-19 Hope Biosciences, Llc Method for generating protein rich conditioned medium
US11111480B2 (en) 2016-04-29 2021-09-07 Hope Biosctences, Llc Culture media for multipotent stem cells
CN106434532A (zh) * 2016-12-22 2017-02-22 叶宗耀 一种培养肝细胞的培养基及其制备方法
CN106957815B (zh) * 2017-03-16 2021-05-07 杨涛 一种用于人类多潜能干细胞的无血清培养基的配方
CN110494559B (zh) * 2017-03-28 2023-10-31 味之素株式会社 不分化地维持用培养基添加剂
CN107119017B (zh) * 2017-05-25 2020-05-26 山东省医药生物技术研究中心 一种骨肉瘤细胞的无血清培养基及其制备方法
JPWO2020067502A1 (ja) * 2018-09-28 2021-08-30 味の素株式会社 動物細胞の培養用添加物、培養用培地および培養方法
US20220145264A1 (en) * 2019-04-05 2022-05-12 The University Of Hong Kong Culture medium for mammalian expanded potential stem cells, composition, and methods thereof
JP7650818B2 (ja) 2019-04-26 2025-03-25 アロジーン セラピューティクス,インコーポレイテッド 同種car t細胞を製造する方法
EP3983481A1 (en) * 2019-06-13 2022-04-20 Corning Incorporated Dissolvable microcarriers for culturing cells and related methods
CN114058573B (zh) * 2020-08-03 2024-04-05 上海原天生物科技有限公司 一种含有生物素的培养基
CN112608892B (zh) * 2020-12-25 2023-11-14 深圳博雅感知药业有限公司 血小板裂解物用于无血清分离和传代培养脐带间充质干细胞的方法
KR20220104321A (ko) 2021-01-18 2022-07-26 주식회사 엑소코바이오 엑소좀 및/또는 세포외 소포체의 생성 촉진 내지는 생산성 향상 방법
US11735303B2 (en) 2021-06-22 2023-08-22 David Haase Apparatus and method for determining a composition of a replacement therapy treatment
JPWO2024253069A1 (enExample) * 2023-06-06 2024-12-12
KR20250140345A (ko) * 2024-03-18 2025-09-25 주식회사 심플플래닛 세포 증식 활성이 우수한 식용 배지 조성물

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JP2993614B2 (ja) * 1990-11-20 1999-12-20 有限会社野々川商事 新規化粧料、グルコサミノグリカン生成促進剤および美白剤
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ES2370012T3 (es) * 2002-01-09 2011-12-12 Alberto Gorrochategui Barrueta Composición para la creación, regeneración y reparación tisular mediante un implante biológico que porta células enriquecido con concentrado de plaquetas y complementos.
US20060223177A1 (en) * 2003-06-27 2006-10-05 Ethicon Inc. Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
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Also Published As

Publication number Publication date
KR20190016612A (ko) 2019-02-18
EP2931875A1 (en) 2015-10-21
HK1215050A1 (en) 2016-08-12
AR093985A1 (es) 2015-07-01
MX364731B (es) 2019-05-06
AU2013359265B2 (en) 2019-01-31
RU2684306C2 (ru) 2019-04-05
WO2014093598A1 (en) 2014-06-19
AU2019202191B2 (en) 2021-01-21
JP2018183195A (ja) 2018-11-22
US20140170748A1 (en) 2014-06-19
AR118040A2 (es) 2021-09-15
AU2019202191A1 (en) 2019-04-18
RU2653449C2 (ru) 2018-05-08
JP6404227B2 (ja) 2018-10-10
RU2018112452A (ru) 2019-03-01
MX2015007594A (es) 2016-03-15
MX2018013469A (es) 2020-11-12
RU2018112452A3 (enExample) 2019-03-01
SG11201504547SA (en) 2015-07-30
CN105026551B (zh) 2020-07-03
KR20150094760A (ko) 2015-08-19
JP6595061B2 (ja) 2019-10-23
EP3382007A1 (en) 2018-10-03
CN111690602A (zh) 2020-09-22
JP2016501026A (ja) 2016-01-18
PH12015501310A1 (en) 2015-09-02
CN105026551A (zh) 2015-11-04
BR112015013685A8 (pt) 2019-11-05
ES2667573T3 (es) 2018-05-11
PH12015501310B1 (en) 2015-09-02
KR101948237B1 (ko) 2019-02-14
CA2895079A1 (en) 2014-06-19
RU2015128276A (ru) 2017-01-23
KR102151588B1 (ko) 2020-09-04
BR112015013685A2 (pt) 2017-07-11
PL2931875T3 (pl) 2018-08-31
EP2931875B1 (en) 2018-03-21
TW201439321A (zh) 2014-10-16
TWI620818B (zh) 2018-04-11
SG10201801924VA (en) 2018-05-30
AU2013359265A1 (en) 2015-07-16

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