ATE80180T1 - Herstellung eines igg-bindenden proteins zum erleichtern der weiterverarbeitung mittels ''protein-engineering''. - Google Patents

Herstellung eines igg-bindenden proteins zum erleichtern der weiterverarbeitung mittels ''protein-engineering''.

Info

Publication number
ATE80180T1
ATE80180T1 AT86850416T AT86850416T ATE80180T1 AT E80180 T1 ATE80180 T1 AT E80180T1 AT 86850416 T AT86850416 T AT 86850416T AT 86850416 T AT86850416 T AT 86850416T AT E80180 T1 ATE80180 T1 AT E80180T1
Authority
AT
Austria
Prior art keywords
recombinant dna
protein
sequence
fragment
dna molecule
Prior art date
Application number
AT86850416T
Other languages
German (de)
English (en)
Inventor
Lars Abrahamsen
Tomas Moks
Bjoern Nilsson
Mathias Uhlen
Original Assignee
Kabigen Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kabigen Ab filed Critical Kabigen Ab
Application granted granted Critical
Publication of ATE80180T1 publication Critical patent/ATE80180T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Diabetes (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT86850416T 1985-12-13 1986-12-02 Herstellung eines igg-bindenden proteins zum erleichtern der weiterverarbeitung mittels ''protein-engineering''. ATE80180T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE8505922A SE8505922D0 (sv) 1985-12-13 1985-12-13 Construction of an igg binding protein to facilitate downstream processing using protein engineering
EP86850416A EP0230869B1 (en) 1985-12-13 1986-12-02 Construction of an igg binding protein to facilitate downstream processing using protein engineering

Publications (1)

Publication Number Publication Date
ATE80180T1 true ATE80180T1 (de) 1992-09-15

Family

ID=20362468

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86850416T ATE80180T1 (de) 1985-12-13 1986-12-02 Herstellung eines igg-bindenden proteins zum erleichtern der weiterverarbeitung mittels ''protein-engineering''.

Country Status (11)

Country Link
US (1) US5143844A (en, 2012)
EP (1) EP0230869B1 (en, 2012)
JP (1) JPH0811069B2 (en, 2012)
AT (1) ATE80180T1 (en, 2012)
AU (1) AU600244B2 (en, 2012)
CA (1) CA1311430C (en, 2012)
DE (1) DE3686646T2 (en, 2012)
DK (1) DK600486A (en, 2012)
ES (1) ES2046177T3 (en, 2012)
GR (1) GR3006178T3 (en, 2012)
SE (1) SE8505922D0 (en, 2012)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK528285D0 (da) * 1985-11-15 1985-11-15 Novo Industri As Peptider samt praeparater indeholdende disse
SE8505921D0 (sv) * 1985-12-13 1985-12-13 Kabigen Ab A method to export gene products to the growth medium of gram negative bacteria
SE8601940D0 (sv) * 1986-04-25 1986-04-25 Kabigen Ab Preparation of fused proteins, antibodies and processes therefor
US5985599A (en) * 1986-05-29 1999-11-16 The Austin Research Institute FC receptor for immunoglobulin
JPH02500329A (ja) * 1987-05-21 1990-02-08 クリエイテイブ・バイオマリキユールズ・インコーポレーテツド ターゲット化多機能蛋白質
JPH082315B1 (en, 2012) * 1987-05-29 1996-01-17 Sagami Chuo Kagaku Kenkyusho
JP2566325B2 (ja) * 1987-11-20 1996-12-25 クリエイティブ・バイオマリキュールズ・インコーポレーテッド 免疫複合体の選択的除去
US5243040A (en) * 1987-11-20 1993-09-07 Creative Biomolecules DNA encoding a protein which enables selective removal of immune complexes
US5084398A (en) * 1987-11-20 1992-01-28 Creative Biomolecules Selective removal of immune complexes
SE8800981D0 (sv) * 1988-03-17 1988-03-17 Nygren Per Ake A recombinant fusion protein, its use and a recombinant vector
SE509359C2 (sv) * 1989-08-01 1999-01-18 Cemu Bioteknik Ab Användning av stabiliserade protein- eller peptidkonjugat för framställning av ett läkemedel
GB8927008D0 (en) * 1989-11-29 1990-01-17 Ciba Geigy Novel process for the production of unfused protein in e.coli
US5681814A (en) * 1990-06-07 1997-10-28 Genentech, Inc. Formulated IGF-I Composition
US5595887A (en) * 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
US6077694A (en) * 1990-09-21 2000-06-20 The United States Of America As Represented By The Department Of Health And Human Services Method for over-expression and rapid purification of biosynthetic proteins
US5863783A (en) * 1991-03-27 1999-01-26 Gist-Brocades, N.V. Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
US5736363A (en) * 1991-07-29 1998-04-07 British Bio-Technology Limited IGF-II analogues
US5989887A (en) * 1992-11-25 1999-11-23 Dsm N.V. Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin
SE9504019D0 (sv) * 1995-11-13 1995-11-13 Pharmacia Ab Method for production of proteins
US5917026A (en) * 1996-02-05 1999-06-29 Loewenadler; Bjoern Fusion proteins of immunopotentiating activity
US5962270A (en) 1996-02-06 1999-10-05 Bionebraska, Inc. Recombinant preparation of calcitonin fragments and use thereof in the preparation of calcitonin and related analogs
WO1998011913A1 (en) * 1996-09-16 1998-03-26 Dalhousie University Use of igf-i for the treatment of polycystic kidney disease and related indications
US6593290B1 (en) 1996-11-01 2003-07-15 Genentech, Inc. Treatment of inner ear hair cells
US6156728A (en) 1996-11-01 2000-12-05 Genentech, Inc. Treatment of inner ear hair cells
US6420518B1 (en) 1997-04-04 2002-07-16 Genetech, Inc. Insulin-like growth factor agonist molecules
US6121416A (en) 1997-04-04 2000-09-19 Genentech, Inc. Insulin-like growth factor agonist molecules
PT1141015E (pt) 1999-01-06 2009-10-13 Genentech Inc Variantes mutantes do factor de crescimento do tipo insulina (igf)i
DK1141014T3 (da) 1999-01-06 2005-04-11 Genentech Inc Insulinlignende vækstfaktor (IGF) i mutantvariant
US6559122B1 (en) 1999-04-08 2003-05-06 Genentech, Inc. Formulated composition
ATE389416T1 (de) 2000-05-16 2008-04-15 Genentech Inc Behandlung von knorpelerkrankungen
US20030157561A1 (en) * 2001-11-19 2003-08-21 Kolkman Joost A. Combinatorial libraries of monomer domains
SE0200943D0 (sv) 2002-03-25 2002-03-25 Amersham Biosciences Ab Mutant protein
US20050003548A1 (en) * 2002-07-22 2005-01-06 Nikolay Korokhov Targeted adenoviral vector displaying immunoglobulin-binding domain and uses thereof
CA2538342C (en) 2003-09-12 2013-01-08 Tercica, Inc. Methods for treatment of insulin-like growth factor-1 (igf-1) deficiency
WO2005033132A2 (en) * 2003-10-03 2005-04-14 Genentech, Inc. Igf binding proteins
DE05733048T1 (de) 2004-04-06 2007-05-10 Affibody Ab Serumalbuminbindende peptidkonjugate zur arzneimittelherstellung
EP2465870A1 (en) 2005-11-07 2012-06-20 Genentech, Inc. Binding polypeptides with diversified and consensus VH/VL hypervariable sequences
RU2470941C2 (ru) 2005-12-02 2012-12-27 Дженентек, Инк. Связывающие полипептиды и их применения
JP2008048607A (ja) 2006-08-22 2008-03-06 Chisso Corp Ｆｃ結合ドメインとカルシウム結合発光蛋白質との融合蛋白質、それをコードする遺伝子およびそれらの用途
GB2442118B (en) 2006-09-19 2011-08-17 Chisso Corp Process for production of recombinant proteins as a soluble form
CA2666053C (en) 2006-12-06 2011-03-29 Repligen Corporation Nucleic acids encoding recombinant protein a
US8198043B2 (en) 2006-12-08 2012-06-12 General Electric Company Two helix binders
US8592555B2 (en) 2008-08-11 2013-11-26 Emd Millipore Corporation Immunoglobulin-binding proteins with improved specificity
SG195555A1 (en) 2008-12-24 2013-12-30 Emd Millipore Corp Caustic stable chromatography ligands
CN102365361B (zh) 2009-03-24 2015-09-02 株式会社钟化 对免疫球蛋白具有亲和性的蛋白质和免疫球蛋白结合性亲和配体
RU2598248C2 (ru) 2009-04-02 2016-09-20 Роше Гликарт Аг Полиспецифичные антитела, включающие антитела полной длины и одноцепочечные фрагменты fab
RU2573915C2 (ru) 2009-09-16 2016-01-27 Дженентек, Инк. Содержащие суперспираль и/или привязку белковые комплексы и их применение
EP2544785B1 (en) * 2010-03-08 2015-09-30 GE Healthcare Bio-Sciences AB Immunoglobulin g fc region binding polypeptide
EP2557157B1 (en) 2010-03-24 2017-02-08 Kaneka Corporation Protein capable of binding specifically to immunoglobulin, and immunoglobulin-binding affinity ligand
AR080793A1 (es) 2010-03-26 2012-05-09 Roche Glycart Ag Anticuerpos biespecificos
WO2011133886A2 (en) 2010-04-23 2011-10-27 Genentech, Inc. Production of heteromultimeric proteins
WO2012021773A1 (en) 2010-08-13 2012-02-16 Genentech, Inc. Antibodies to il-1beta and il-18, for treatment of disease
CN103068846B9 (zh) 2010-08-24 2016-09-28 弗·哈夫曼-拉罗切有限公司 包含二硫键稳定性Fv片段的双特异性抗体
EP2654915B1 (en) 2010-12-20 2018-10-24 GE Healthcare BioProcess R&D AB Affinity chromatography matrix
JP5974343B2 (ja) 2010-12-20 2016-08-23 ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ アフィニティークロマトグラフィーマトリックス
SG191153A1 (en) 2010-12-23 2013-07-31 Hoffmann La Roche Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery
MX355255B (es) 2011-02-04 2018-04-11 Genentech Inc Variantes de fc y métodos para su producción.
US10689447B2 (en) 2011-02-04 2020-06-23 Genentech, Inc. Fc variants and methods for their production
KR20140007394A (ko) * 2011-03-25 2014-01-17 가부시키가이샤 가네카 면역 글로불린 결합성 신규 폴리펩티드
SG10201604554WA (en) 2011-06-08 2016-07-28 Emd Millipore Corp Chromatography matrices including novel staphylococcus aureus protein a based ligands
CN104093744A (zh) 2011-10-11 2014-10-08 弗·哈夫曼-拉罗切有限公司 双特异性抗体的改进的组装
WO2013119966A2 (en) 2012-02-10 2013-08-15 Genentech, Inc. Single-chain antibodies and other heteromultimers
JP6420756B2 (ja) * 2012-03-28 2018-11-07 ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ アフィニティークロマトグラフィーマトリックス
WO2014001325A1 (en) 2012-06-27 2014-01-03 F. Hoffmann-La Roche Ag Method for making antibody fc-region conjugates comprising at least one binding entity that specifically binds to a target and uses thereof
RU2639287C2 (ru) 2012-06-27 2017-12-20 Ф. Хоффманн-Ля Рош Аг Способ отбора и получения высокоселективных и мультиспецифичных нацеливающих групп с заданными свойствами, включающих по меньшей мере две различные связывающие группировки, и их применения
EP3019520B1 (en) 2013-07-10 2019-12-11 GE Healthcare BioProcess R&D AB Mutated immunoglobulin-binding polypeptides
EP3050902B1 (en) 2013-09-27 2018-11-21 Kaneka Corporation Process for producing porous cellulose beads using alkali aqueous solution, and porous cellulose beads produced by this process
JP6715186B2 (ja) 2014-05-06 2020-07-01 ジェネンテック， インコーポレイテッド 哺乳動物細胞を用いたヘテロ多量体タンパク質の生成
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
WO2016087416A1 (en) 2014-12-03 2016-06-09 F. Hoffmann-La Roche Ag Multispecific antibodies
CN108064286A (zh) 2015-01-26 2018-05-22 株式会社钟化 突变型免疫球蛋白κ链可变区结合性肽
JPWO2016121701A1 (ja) 2015-01-26 2017-11-09 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
JP6424101B2 (ja) * 2015-01-30 2018-11-14 Jnc株式会社 Ｆｃ結合ドメインとカルシウム結合発光蛋白質との融合蛋白質、それをコードする遺伝子およびそれらの用途
JP6805831B2 (ja) 2015-02-05 2020-12-23 三菱ケミカル株式会社 免疫グロブリンに親和性を有するタンパク質、およびそれを用いたアフィニティ分離剤、液体クロマトグラフィー用カラム
WO2017195638A1 (ja) 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
JP6987424B2 (ja) 2016-05-11 2022-01-05 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックスを洗浄および／または消毒する方法
WO2017194597A1 (en) 2016-05-11 2017-11-16 Ge Healthcare Bioprocess R&D Ab Separation matrix
JP7106187B2 (ja) 2016-05-11 2022-07-26 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックスを保存する方法
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
CA3072288A1 (en) 2017-08-07 2019-02-14 Repligen Corporation Fc binding proteins with cysteine in the c-terminal helical region
EP3521304B9 (en) 2018-02-01 2023-10-04 Repligen Corporation Fc binding proteins with cysteine in the c-terminal helical region
GB202018588D0 (en) 2020-11-26 2021-01-13 Cytiva Bioprocess R & D Ab Separation matrix
GB202113626D0 (en) 2021-09-24 2021-11-10 Cytiva Bioprocess R & D Ab FC binding polypeptides

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4769326A (en) * 1980-02-29 1988-09-06 The Regents Of The University Of California Expression linkers
SE8300693L (sv) * 1983-02-09 1984-08-10 Sven Lofdahl Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta

Also Published As

Publication number Publication date
DE3686646T2 (de) 1993-04-08
DK600486A (da) 1987-06-14
CA1311430C (en) 1992-12-15
AU6633186A (en) 1987-06-18
SE8505922D0 (sv) 1985-12-13
DK600486D0 (da) 1986-12-12
AU600244B2 (en) 1990-08-09
EP0230869A3 (en) 1988-08-03
GR3006178T3 (en, 2012) 1993-06-21
EP0230869A2 (en) 1987-08-05
JPS62190087A (ja) 1987-08-20
DE3686646D1 (de) 1992-10-08
EP0230869B1 (en) 1992-09-02
ES2046177T3 (es) 1994-02-01
JPH0811069B2 (ja) 1996-02-07
US5143844A (en) 1992-09-01

Similar Documents

Publication Publication Date Title
ATE80180T1 (de) Herstellung eines igg-bindenden proteins zum erleichtern der weiterverarbeitung mittels ''protein-engineering''.
Kodaira et al. The dnaX gene encodes the DNA polymerase III holoenzyme τ subunit, precursor of the γ subunit, the dnaZ gene product
Rüther et al. Easy identification of cDNA clones.
IL173399A0 (en) Circular dna molecule with conditional origin of replication which contains nucleic acid of interest and selection marker but does not encode its replication initiation protein, method for preparing the same and use thereof in gene therapy
Pettigrew et al. Escherichia coli glycerol kinase. Cloning and sequencing of the glpK gene and the primary structure of the enzyme.
IL57491A (en) Recombinant dna,methods for the production thereof and proteins or polypeptides produced thereby
IE63991B1 (en) Novel fusion proteins and their purification
ZA822855B (en) Bacillus cloning vectors,recombinant dna molecules,bacillus hosts transformed with them and methods for expressing foreign dna sequences and producing polypeptides coded thereby
ATE294871T1 (de) Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
DK1005540T3 (da) IKK-beta-proteiner, nukleinsyrer og fremgangsmåder
NZ510367A (en) Interleukin 17-like receptor protein nucleic acid sequence encoding the interleukin receptor protein and method of expressing protein in recombinant vector
Lodwick et al. DNA sequence analysis of the imp UV protection and mutation operon of the plasmid TP110: identification of a third gene
IL88151A (en) Interleukin-7 protein, dna sequences encoding it, its preparation and compositions containing it
JPS6420095A (en) Polypeptide having anti-blood coagulating action
Miele et al. Gene organization and nucleotide sequence of the primase region of IncP plasmids RP4 and R751
DK0805204T3 (da) Bitestikel - specifikt receptorprotein og anvendelse heraf
DE60029791D1 (de) Rezeptor eines neurotrophen faktors (gfr-alpha-4)
Prieto et al. Characterization, overproduction and purification of the product of gene 1 of Bacillus subtilis phage φ29
KR900700615A (ko) 사람 호중구 화학주성 인자 폴리펩티드의 제조방법
Taylor et al. Similarities in the heavy and light chains of tetanus toxin suggested by their amino acid compositions
Renlund et al. Peptide mapping of HIV polypeptides expressed in Escherichia coli: Quality control of different batches and identification of tryptic fragments containing residues of aromatic amino acids or cysteine
DK551786D0 (da) Rekombinant-dna-sekvens, der koder for aminosyresekvenser i et toksin, expressionssystem omfattende samme og vaertsceller transformeret hermed
Morishita et al. A brain-specific γ subunit of G protein freed from the corresponding β subunit under non-denaturing conditions
WO1998025957A3 (en) Human prt1-like subunit protein (hprt1) and human eif4g-like protein (p97) genes
EP0213463B1 (en) Interleukin 2 receptor and a method for its production

Legal Events

Date Code Title Description
REN Ceased due to non-payment of the annual fee