SE8505921D0 - A method to export gene products to the growth medium of gram negative bacteria - Google Patents

A method to export gene products to the growth medium of gram negative bacteria

Info

Publication number
SE8505921D0
SE8505921D0 SE8505921A SE8505921A SE8505921D0 SE 8505921 D0 SE8505921 D0 SE 8505921D0 SE 8505921 A SE8505921 A SE 8505921A SE 8505921 A SE8505921 A SE 8505921A SE 8505921 D0 SE8505921 D0 SE 8505921D0
Authority
SE
Sweden
Prior art keywords
sub
recombinant dna
structural gene
protein
gram
Prior art date
Application number
SE8505921A
Other languages
English (en)
Inventor
L Abrahamsen
T M B Nilsson
M Uhlen
Original Assignee
Kabigen Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Publication of SE8505921D0 publication Critical patent/SE8505921D0/sv
Priority to SE8505921A priority Critical patent/SE8505921D0/sv
Application filed by Kabigen Ab filed Critical Kabigen Ab
Priority to EP86850415A priority patent/EP0225860B1/en
Priority to DE8686850415T priority patent/DE3687112T2/de
Priority to AT86850415T priority patent/ATE82320T1/de
Priority to ES86850415T priority patent/ES2052497T3/es
Priority to AU66332/86A priority patent/AU597317B2/en
Priority to DK198606003A priority patent/DK175020B1/da
Priority to CA000525185A priority patent/CA1314010C/en
Priority to JP61296510A priority patent/JPH082314B2/ja
Priority to US07/602,935 priority patent/US5156959A/en
Priority to GR920403005T priority patent/GR3006597T3/el
Priority to JP6079184A priority patent/JP2504723B2/ja

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors (Somatomedins), e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
SE8505921A 1985-12-13 1985-12-13 A method to export gene products to the growth medium of gram negative bacteria SE8505921D0 (sv)

Priority Applications (12)

Application Number Priority Date Filing Date Title
SE8505921A SE8505921D0 (sv) 1985-12-13 1985-12-13 A method to export gene products to the growth medium of gram negative bacteria
EP86850415A EP0225860B1 (en) 1985-12-13 1986-12-02 A method to export gene products to the growth medium of gram negative bacteria
DE8686850415T DE3687112T2 (de) 1985-12-13 1986-12-02 Verfahren zur ausscheidung von genprodukten in das wachstumsmedium von gram-negativen bakterien.
AT86850415T ATE82320T1 (de) 1985-12-13 1986-12-02 Verfahren zur ausscheidung von genprodukten in das wachstumsmedium von gram-negativen bakterien.
ES86850415T ES2052497T3 (es) 1985-12-13 1986-12-02 Un metodo para exportar productos de gen al medio de crecimiento de bacterias gram-negativas.
AU66332/86A AU597317B2 (en) 1985-12-13 1986-12-09 A method to export gene products to the growth medium of gram negative bacteria
JP61296510A JPH082314B2 (ja) 1985-12-13 1986-12-12 グラム陰性菌の増殖培地に遺伝子生成物を輸送する方法
DK198606003A DK175020B1 (da) 1985-12-13 1986-12-12 Fremgangsåde til udtrykkelse og ekstracellulær udskillelse af proteiner i G(-)bakterie, rekombinant DNA-konstruktion og plasmidvektor kodende herfor, samt G(-)bakterie indeholdende disse
CA000525185A CA1314010C (en) 1985-12-13 1986-12-12 Method to export gene products to the growth medium of gram negative bacteria
US07/602,935 US5156959A (en) 1985-12-13 1990-10-24 Method to export gene products to the growth medium of gram negative bacteria
GR920403005T GR3006597T3 (sv) 1985-12-13 1992-12-21
JP6079184A JP2504723B2 (ja) 1985-12-13 1994-03-28 組換体ｄｎａとこれを含むプラスミドベクタ―とこのような組換体ｄｎａを保持する大腸菌

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8505921A SE8505921D0 (sv) 1985-12-13 1985-12-13 A method to export gene products to the growth medium of gram negative bacteria

Publications (1)

Publication Number Publication Date
SE8505921D0 true SE8505921D0 (sv) 1985-12-13

Family

ID=20362467

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8505921A SE8505921D0 (sv) 1985-12-13 1985-12-13 A method to export gene products to the growth medium of gram negative bacteria

Country Status (11)

Country Link
US (1) US5156959A (sv)
EP (1) EP0225860B1 (sv)
JP (2) JPH082314B2 (sv)
AT (1) ATE82320T1 (sv)
AU (1) AU597317B2 (sv)
CA (1) CA1314010C (sv)
DE (1) DE3687112T2 (sv)
DK (1) DK175020B1 (sv)
ES (1) ES2052497T3 (sv)
GR (1) GR3006597T3 (sv)
SE (1) SE8505921D0 (sv)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8601940D0 (sv) * 1986-04-25 1986-04-25 Kabigen Ab Preparation of fused proteins, antibodies and processes therefor
JPH03505401A (ja) * 1988-06-27 1991-11-28 ジェネックス・コーポレーション 組換えタンパク質の培養培地への熱放出
JPH04500754A (ja) * 1988-09-30 1992-02-13 アライド―シグナル・インコーポレーテッド 異種遺伝子発現のための改良細菌株
EP0550771B1 (en) * 1991-07-25 1999-09-29 Oriental Yeast Co., Ltd. Immunoglobulin-binding artificial protein
DE69403654T2 (de) * 1993-03-29 1997-12-11 Du Pont Ein verfahren zur erhöhung der produktion von biologisch aktiven rekombinanten proteinen
US6958148B1 (en) 1998-01-20 2005-10-25 Pericor Science, Inc. Linkage of agents to body tissue using microparticles and transglutaminase
KR100312456B1 (ko) 1999-03-13 2001-11-03 윤덕용 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자
JP3553858B2 (ja) 1999-08-25 2004-08-11 東洋紡績株式会社 血管網類似構造体を有する細胞培養用モジュール
JP4220756B2 (ja) 2002-10-28 2009-02-04 極東製薬工業株式会社 培養器、培養器の製造方法及び培養方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
NO172595C (no) * 1983-02-07 1993-08-11 Battelle Memorial Institute Fremgangsmaate og ekspresjonsplasmid for fremstilling av hemagglutinin under kontroll av drosophila hsp-promoter
SE8300693L (sv) * 1983-02-09 1984-08-10 Sven Lofdahl Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta
US4472302A (en) * 1983-03-09 1984-09-18 Merck & Co., Inc. Heat shock process for the isolation of bacterial protein
US4624922A (en) * 1983-12-09 1986-11-25 Rikagaku Kenkyusho Plasmid, method for construction of the same, microorganism carrying the plasmid and method for cultivation of the microorganism
FR2556740B1 (fr) * 1983-12-14 1987-02-27 Merieux Inst Nouveaux mutants d'escherichia coli, capables d'excreter dans le milieu extracellulaire des proteines periplasmiques, leur obtention et leur application a la preparation de beta-lactamases ou de proteines hybrides de beta-lactamases
SE8505922D0 (sv) * 1985-12-13 1985-12-13 Kabigen Ab Construction of an igg binding protein to facilitate downstream processing using protein engineering

Also Published As

Publication number Publication date
JPH082314B2 (ja) 1996-01-17
JP2504723B2 (ja) 1996-06-05
EP0225860A3 (en) 1988-10-19
AU597317B2 (en) 1990-05-31
AU6633286A (en) 1987-06-18
DK600386D0 (da) 1986-12-12
DE3687112T2 (de) 1993-06-03
JPS62190088A (ja) 1987-08-20
CA1314010C (en) 1993-03-02
EP0225860B1 (en) 1992-11-11
DK600386A (da) 1987-06-14
ES2052497T3 (es) 1994-07-16
JPH0746988A (ja) 1995-02-21
GR3006597T3 (sv) 1993-06-30
EP0225860A2 (en) 1987-06-16
US5156959A (en) 1992-10-20
ATE82320T1 (de) 1992-11-15
DE3687112D1 (de) 1992-12-17
DK175020B1 (da) 2004-04-26

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