ATE82320T1 - Verfahren zur ausscheidung von genprodukten in das wachstumsmedium von gram-negativen bakterien. - Google Patents
Verfahren zur ausscheidung von genprodukten in das wachstumsmedium von gram-negativen bakterien.Info
- Publication number
- ATE82320T1 ATE82320T1 AT86850415T AT86850415T ATE82320T1 AT E82320 T1 ATE82320 T1 AT E82320T1 AT 86850415 T AT86850415 T AT 86850415T AT 86850415 T AT86850415 T AT 86850415T AT E82320 T1 ATE82320 T1 AT E82320T1
- Authority
- AT
- Austria
- Prior art keywords
- sub
- recombinant dna
- structural gene
- protein
- gram
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 12
- 241000894006 Bacteria Species 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- 230000029142 excretion Effects 0.000 title 1
- 239000001963 growth medium Substances 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 abstract 6
- 108020004511 Recombinant DNA Proteins 0.000 abstract 4
- 238000010276 construction Methods 0.000 abstract 4
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 230000000875 corresponding effect Effects 0.000 abstract 1
- 230000028564 filamentous growth Effects 0.000 abstract 1
- 239000013600 plasmid vector Substances 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
- 230000005945 translocation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
- C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Chemical & Material Sciences (AREA)
- Virology (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8505921A SE8505921D0 (sv) | 1985-12-13 | 1985-12-13 | A method to export gene products to the growth medium of gram negative bacteria |
| EP86850415A EP0225860B1 (de) | 1985-12-13 | 1986-12-02 | Verfahren zur Ausscheidung von Genprodukten in das Wachstumsmedium von gram-negativen Bakterien |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE82320T1 true ATE82320T1 (de) | 1992-11-15 |
Family
ID=20362467
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT86850415T ATE82320T1 (de) | 1985-12-13 | 1986-12-02 | Verfahren zur ausscheidung von genprodukten in das wachstumsmedium von gram-negativen bakterien. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US5156959A (de) |
| EP (1) | EP0225860B1 (de) |
| JP (2) | JPH082314B2 (de) |
| AT (1) | ATE82320T1 (de) |
| AU (1) | AU597317B2 (de) |
| CA (1) | CA1314010C (de) |
| DE (1) | DE3687112T2 (de) |
| DK (1) | DK175020B1 (de) |
| ES (1) | ES2052497T3 (de) |
| GR (1) | GR3006597T3 (de) |
| SE (1) | SE8505921D0 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE8601940D0 (sv) * | 1986-04-25 | 1986-04-25 | Kabigen Ab | Preparation of fused proteins, antibodies and processes therefor |
| JPH03505401A (ja) * | 1988-06-27 | 1991-11-28 | ジェネックス・コーポレーション | 組換えタンパク質の培養培地への熱放出 |
| JPH04500754A (ja) * | 1988-09-30 | 1992-02-13 | アライド―シグナル・インコーポレーテッド | 異種遺伝子発現のための改良細菌株 |
| EP0550771B1 (de) * | 1991-07-25 | 1999-09-29 | Oriental Yeast Co., Ltd. | Künstliches immunoglobulin-bindendes protein |
| EP0695359B1 (de) * | 1993-03-29 | 1997-06-04 | E.I. Du Pont De Nemours & Company Incorporated | Ein verfahren zur erhöhung der produktion von biologisch aktiven rekombinanten proteinen |
| US6958148B1 (en) | 1998-01-20 | 2005-10-25 | Pericor Science, Inc. | Linkage of agents to body tissue using microparticles and transglutaminase |
| KR100312456B1 (ko) | 1999-03-13 | 2001-11-03 | 윤덕용 | 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자 |
| JP3553858B2 (ja) | 1999-08-25 | 2004-08-11 | 東洋紡績株式会社 | 血管網類似構造体を有する細胞培養用モジュール |
| JP4220756B2 (ja) | 2002-10-28 | 2009-02-04 | 極東製薬工業株式会社 | 培養器、培養器の製造方法及び培養方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4338397A (en) * | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
| IE56720B1 (en) * | 1983-02-07 | 1991-11-20 | Battelle Memorial Institute | Methods and compositions for expression of competent eukaryotic gene products |
| SE8300693L (sv) * | 1983-02-09 | 1984-08-10 | Sven Lofdahl | Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta |
| US4472302A (en) * | 1983-03-09 | 1984-09-18 | Merck & Co., Inc. | Heat shock process for the isolation of bacterial protein |
| US4624922A (en) * | 1983-12-09 | 1986-11-25 | Rikagaku Kenkyusho | Plasmid, method for construction of the same, microorganism carrying the plasmid and method for cultivation of the microorganism |
| FR2556740B1 (fr) * | 1983-12-14 | 1987-02-27 | Merieux Inst | Nouveaux mutants d'escherichia coli, capables d'excreter dans le milieu extracellulaire des proteines periplasmiques, leur obtention et leur application a la preparation de beta-lactamases ou de proteines hybrides de beta-lactamases |
| SE8505922D0 (sv) * | 1985-12-13 | 1985-12-13 | Kabigen Ab | Construction of an igg binding protein to facilitate downstream processing using protein engineering |
-
1985
- 1985-12-13 SE SE8505921A patent/SE8505921D0/xx unknown
-
1986
- 1986-12-02 EP EP86850415A patent/EP0225860B1/de not_active Expired
- 1986-12-02 ES ES86850415T patent/ES2052497T3/es not_active Expired - Lifetime
- 1986-12-02 AT AT86850415T patent/ATE82320T1/de not_active IP Right Cessation
- 1986-12-02 DE DE8686850415T patent/DE3687112T2/de not_active Expired - Fee Related
- 1986-12-09 AU AU66332/86A patent/AU597317B2/en not_active Ceased
- 1986-12-12 JP JP61296510A patent/JPH082314B2/ja not_active Expired - Lifetime
- 1986-12-12 DK DK198606003A patent/DK175020B1/da not_active IP Right Cessation
- 1986-12-12 CA CA000525185A patent/CA1314010C/en not_active Expired - Fee Related
-
1990
- 1990-10-24 US US07/602,935 patent/US5156959A/en not_active Expired - Lifetime
-
1992
- 1992-12-21 GR GR920403005T patent/GR3006597T3/el unknown
-
1994
- 1994-03-28 JP JP6079184A patent/JP2504723B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DK175020B1 (da) | 2004-04-26 |
| EP0225860B1 (de) | 1992-11-11 |
| JPH0746988A (ja) | 1995-02-21 |
| DE3687112T2 (de) | 1993-06-03 |
| JPH082314B2 (ja) | 1996-01-17 |
| DE3687112D1 (de) | 1992-12-17 |
| JP2504723B2 (ja) | 1996-06-05 |
| JPS62190088A (ja) | 1987-08-20 |
| CA1314010C (en) | 1993-03-02 |
| ES2052497T3 (es) | 1994-07-16 |
| SE8505921D0 (sv) | 1985-12-13 |
| DK600386A (da) | 1987-06-14 |
| AU6633286A (en) | 1987-06-18 |
| DK600386D0 (da) | 1986-12-12 |
| EP0225860A2 (de) | 1987-06-16 |
| US5156959A (en) | 1992-10-20 |
| GR3006597T3 (de) | 1993-06-30 |
| AU597317B2 (en) | 1990-05-31 |
| EP0225860A3 (en) | 1988-10-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69534822D1 (de) | Verfahren zur bakteriellen herstellung von heterologen polypeptiden mit disulfidbrücke | |
| DK102886D0 (da) | Proteinfaktorer | |
| NZ190524A (en) | Recombinant dna method for producing specific protein or polypeptide cleaving of bacterial gene for extracellular or periplasmic carrier protein | |
| KR880014110A (ko) | 인체 프로아포지방단백질 a-i의 발현 | |
| IE801214L (en) | Microbial expression of quasi-synthetic genes | |
| DE3586386T2 (de) | Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung. | |
| IE820069L (en) | Recombinant dna encoding a polypeptide | |
| FI853056A0 (fi) | Menetelmä hirudiinin vaikutusta omaavan polypeptidin valmistamiseksi | |
| ES2000278A6 (es) | Procedimiento para preparar una proteina de fusion y para preparar una proteina eucariotica. | |
| ATE82320T1 (de) | Verfahren zur ausscheidung von genprodukten in das wachstumsmedium von gram-negativen bakterien. | |
| JO1694B1 (en) | New chemical products | |
| FR2645174B1 (fr) | Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant | |
| ATE91717T1 (de) | Verfahren zur herstellung von proteinen in loeslicher form. | |
| DE3850456D1 (de) | Klonierung des isoamylaseenzym kodierenden Gens und seine Verwendung zur Herstellung dieses Enzyms. | |
| ATE122097T1 (de) | Expressionsvektor und verfahren zur regulierbaren herstellung von proteinen in eukaryonten. | |
| KR840004163A (ko) | 효모에 의한 이종성 단백질의 표현, 처리 및 분비방법 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| EEIH | Change in the person of patent owner | ||
| REN | Ceased due to non-payment of the annual fee |