ES2046177T3 - Construccion de una proteina enlazante a igg para facilitar el tratamiento aguas abajo utilizando ingenieria de proteinas. - Google Patents

Construccion de una proteina enlazante a igg para facilitar el tratamiento aguas abajo utilizando ingenieria de proteinas.

Info

Publication number
ES2046177T3
ES2046177T3 ES198686850416T ES86850416T ES2046177T3 ES 2046177 T3 ES2046177 T3 ES 2046177T3 ES 198686850416 T ES198686850416 T ES 198686850416T ES 86850416 T ES86850416 T ES 86850416T ES 2046177 T3 ES2046177 T3 ES 2046177T3
Authority
ES
Spain
Prior art keywords
recombinant dna
protein
sequence
fragment
dna molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198686850416T
Other languages
English (en)
Inventor
Lars Abrahamsen
Tomas Moks
Bjorn Nilsson
Mathias Uhlen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Health AB
Original Assignee
Kabi Pharmacia AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kabi Pharmacia AB filed Critical Kabi Pharmacia AB
Application granted granted Critical
Publication of ES2046177T3 publication Critical patent/ES2046177T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

LA INVENCION SE REFIERE A: UN FRAGMENTO RECOMBINANTE DE ADN(Z) QUE CODIFICA UN DOMINIO DE UNION A INMUNOGLOBULINA G RELACIONADO CON LA PROTEINA A ESTAFILOCOCICA, QUE SE CARACTERIZA PORQUE LA METIONINA CODON DE DICHO FRAGMENTO HA SIDO REEMPLAZADA POR UN CODON DE OTRO RESTO DE AMINOACIDO LO QUE POSIBILITA LA EXPRESION DE UNA PROTEINA EXENTA DE METIONINA; UNA SECUENCIA DE ADN RECOMBINANTE QUE CONTIENE AL MENOS DOS DE TALES FRAGMENTOS; UNA SECUENCIA DE ADN RECOMBINANTE QUE CONTIENE DICHOS FRAGMENTOS PRECEDIDOS DE UNA SECUENCIA DE SEÑAL Y SEGUIDOS DE UNA SECUENCIA NUCLEOTIDA QUE CODIFICA LA SECUENCIA AMINO-ACIDO: ALA-GLU-HIS-ASP-GLU -ALA; UNA MOLECULA DE ADN RECOMBINANTE QUE CONTIENE DICHA SECUENCIA DE ADN RECOMBINANTE Y FUSIONADA EN 3'' A NIVEL DE ADN UN GEN DE PRODUCCION, QUE PUEDE EXPRESAR UNA PROTEINA; UN PROCESO PARA DESDOBLAR UNA PROTEINA FUNDIDA EXPRESADA EN UN SISTEMA BIOLOGICO POR DICHA MOLECULA RECOMBINANTE DE ADN; UN VECTOR PLASMIDICO QUE CONTIENE DICHA MOLECULA DE ADN RECOMBINANTE; Y UNA CELULA BACTERIANA QUE LLEVA LA MOLECULA DE ADN RECOMBINANTE.
ES198686850416T 1985-12-13 1986-12-02 Construccion de una proteina enlazante a igg para facilitar el tratamiento aguas abajo utilizando ingenieria de proteinas. Expired - Lifetime ES2046177T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8505922A SE8505922D0 (sv) 1985-12-13 1985-12-13 Construction of an igg binding protein to facilitate downstream processing using protein engineering

Publications (1)

Publication Number Publication Date
ES2046177T3 true ES2046177T3 (es) 1994-02-01

Family

ID=20362468

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198686850416T Expired - Lifetime ES2046177T3 (es) 1985-12-13 1986-12-02 Construccion de una proteina enlazante a igg para facilitar el tratamiento aguas abajo utilizando ingenieria de proteinas.

Country Status (11)

Country Link
US (1) US5143844A (es)
EP (1) EP0230869B1 (es)
JP (1) JPH0811069B2 (es)
AT (1) ATE80180T1 (es)
AU (1) AU600244B2 (es)
CA (1) CA1311430C (es)
DE (1) DE3686646T2 (es)
DK (1) DK600486A (es)
ES (1) ES2046177T3 (es)
GR (1) GR3006178T3 (es)
SE (1) SE8505922D0 (es)

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US4769326A (en) * 1980-02-29 1988-09-06 The Regents Of The University Of California Expression linkers
SE8300693L (sv) * 1983-02-09 1984-08-10 Sven Lofdahl Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta

Also Published As

Publication number Publication date
GR3006178T3 (es) 1993-06-21
DE3686646D1 (de) 1992-10-08
JPH0811069B2 (ja) 1996-02-07
DE3686646T2 (de) 1993-04-08
EP0230869A3 (en) 1988-08-03
ATE80180T1 (de) 1992-09-15
SE8505922D0 (sv) 1985-12-13
JPS62190087A (ja) 1987-08-20
CA1311430C (en) 1992-12-15
US5143844A (en) 1992-09-01
AU600244B2 (en) 1990-08-09
EP0230869A2 (en) 1987-08-05
EP0230869B1 (en) 1992-09-02
AU6633186A (en) 1987-06-18
DK600486A (da) 1987-06-14
DK600486D0 (da) 1986-12-12

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