KR900700615A - 사람 호중구 화학주성 인자 폴리펩티드의 제조방법 - Google Patents
사람 호중구 화학주성 인자 폴리펩티드의 제조방법Info
- Publication number
- KR900700615A KR900700615A KR1019890702514A KR890702514A KR900700615A KR 900700615 A KR900700615 A KR 900700615A KR 1019890702514 A KR1019890702514 A KR 1019890702514A KR 890702514 A KR890702514 A KR 890702514A KR 900700615 A KR900700615 A KR 900700615A
- Authority
- KR
- South Korea
- Prior art keywords
- dna
- sequence represented
- nucleotide sequence
- formula
- chemotactic factor
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims 7
- 108090001007 Interleukin-8 Proteins 0.000 title claims 5
- 102000004890 Interleukin-8 Human genes 0.000 title claims 5
- 239000002773 nucleotide Substances 0.000 claims 7
- 125000003729 nucleotide group Chemical group 0.000 claims 7
- 229920001184 polypeptide Polymers 0.000 claims 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims 3
- 241000588724 Escherichia coli Species 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims 1
- 150000001413 amino acids Chemical group 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 150000001450 anions Chemical class 0.000 claims 1
- 238000005277 cation exchange chromatography Methods 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000006378 damage Effects 0.000 claims 1
- 238000002523 gelfiltration Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 238000005185 salting out Methods 0.000 claims 1
- -1 salting out Chemical class 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 238000000108 ultra-filtration Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5421—IL-8
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (10)
- 사람 호중구 화학주성 인자 폴리펩티드의 제조방법으로서, 상기 폴리펩티드를 코드화하는 DNA가 삽입되어 있는 발현벡터로 형질전환된 세포를 배양함에 의한 방법.
- 제1항에 있어서, 사람 호중구 화학주성 인자 폴리펩티드가 다음식[I]에 의해 표시되는 아미노산 배열로 이루어지는 폴리펩티드인 것을 특징으로 하는 방법.
- 제1항에 있어서, 사람 호중구 화학주성 인자 폴리펩티드를 코드화하는 DNA가 다음식 [A]로 표시되는 염기배열, 또는 그의 축퇴성 배열로 이루어지는 DNA인 것을 특징으로 하는 방법.
- 제1항에 있어서, 형질전환된 세포가 대장균인 것을 특징으로 하는 방법.
- 제1항에 있어서, 발현벡터로서 재조합 플라스미드 pHNP101을 사용하는 것을 특징으로 하는 방법.
- 제3항에 있어서, 도시된 식[A]로 표시되는 염기 배열 및 상기 식[A]로 표시된 염기배열 위쪽에 다음식 [B]로 표시되는 염기배열을 가지고 있는 DNA.5′-XGGAGGTTTYATTATG-3′ 식 [B](상기식에서, X는 (A)X를 의미하고, x는 1내지 5이며, Y는 (A)y(T)Z를 의미하고, y는 0 내지 3이며 z는 0또는 1이다.)
- 제3항에 도시된 식[A]로 표시되는 염기배열 및 상기 식[A]로 표시된 염기배열의 위쪽에 다음식[A]로 표시된 염기배열을 가지고 있는 DNA.5′-AAAAGGAGGTTTAAATTATG-3' 식 [C]
- 제6항 또는 7항에 있어서, 제6항 또는 7항의 DNA의 위쪽에 대장균에서의 전사를 제어하는 프로모터활성을 활성을 가지고 있는 DNA가 추가로 연결되어 있는 거을 특징으로 하는 DNA.
- 제8항에 있어서, 프로모터가 트립토판 오페론의 프로모터인 것을 특징으로 하는 DNA.
- 제1항 내지 5항중 어느 하나의 방법에 의하여 제조되는 사람 호중구 화학주성 인자폴리펩티드의 정제방법에 있어서, 상기 폴리펩티드의 처리에 있어서 다음 단계, 형질전환체의 파괴, 핵산을 제거하기 위한 처리, 염석, 음이온 및/또는 양이온 교환 크로마토그래피, 한외여과, 및 겔 여과의 조합에 의하여 이루어지는 것을 특징으로 하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18916488A | 1988-05-02 | 1988-05-02 | |
| US189,164 | 1988-05-02 | ||
| PCT/JP1989/000437 WO1989010962A1 (en) | 1988-05-02 | 1989-04-26 | Process for producing a human neutrophil chemotactic factor polypeptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR900700615A true KR900700615A (ko) | 1990-08-16 |
Family
ID=22696203
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019890702514A KR900700615A (ko) | 1988-05-02 | 1989-04-26 | 사람 호중구 화학주성 인자 폴리펩티드의 제조방법 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5306627A (ko) |
| EP (1) | EP0413818B1 (ko) |
| JP (1) | JPH03505037A (ko) |
| KR (1) | KR900700615A (ko) |
| AT (1) | ATE116366T1 (ko) |
| DE (1) | DE68920321T2 (ko) |
| WO (1) | WO1989010962A1 (ko) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL194982C (nl) | 1987-11-19 | 2003-04-10 | Novartis Ag | Neutrofiel-activerende factor (NAF) in geïsoleerde vorm, werkwijze voor het bereiden van NAF, expressievector met een codering voor NAF en een NAF bevattend farmaceutisch preparaat. |
| US5652338A (en) | 1988-03-16 | 1997-07-29 | The United States Of America As Represented By The Department Of Health And Human Services | Neutrophil chemotactic factor |
| WO1991008231A1 (en) * | 1989-11-29 | 1991-06-13 | Brigham And Women's Hospital | [Ala IL-8]77 AS A LEUKOCYTE ADHESION INHIBITOR |
| US5241049A (en) * | 1989-12-22 | 1993-08-31 | Zymogenetics, Inc. | Neutrophil chemoattractants |
| ATE527278T1 (de) | 2002-12-16 | 2011-10-15 | Genmab As | Humane monoklonale antikörper gegen interleukin 8 (il-8) |
| JP2007006419A (ja) * | 2005-06-27 | 2007-01-11 | Pioneer Electronic Corp | スピーカ用ダンパ、スピーカおよびスピーカの組立方法 |
| SG11202005323SA (en) | 2018-01-12 | 2020-07-29 | Bristol Myers Squibb Co | Combination therapy with anti-il-8 antibodies and anti-pd-1 antibodies for treating cancer |
-
1989
- 1989-04-26 WO PCT/JP1989/000437 patent/WO1989010962A1/en active IP Right Grant
- 1989-04-26 JP JP1504941A patent/JPH03505037A/ja active Pending
- 1989-04-26 DE DE68920321T patent/DE68920321T2/de not_active Expired - Lifetime
- 1989-04-26 AT AT89905179T patent/ATE116366T1/de not_active IP Right Cessation
- 1989-04-26 EP EP89905179A patent/EP0413818B1/en not_active Expired - Lifetime
- 1989-04-26 KR KR1019890702514A patent/KR900700615A/ko not_active Application Discontinuation
-
1991
- 1991-06-04 US US07/711,275 patent/US5306627A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH03505037A (ja) | 1991-11-07 |
| DE68920321T2 (de) | 1995-05-24 |
| DE68920321D1 (de) | 1995-02-09 |
| WO1989010962A1 (en) | 1989-11-16 |
| EP0413818B1 (en) | 1994-12-28 |
| EP0413818A1 (en) | 1991-02-27 |
| US5306627A (en) | 1994-04-26 |
| ATE116366T1 (de) | 1995-01-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |