AR112756A1 - Incorporación de nucleótidos no naturales, y su método - Google Patents

Incorporación de nucleótidos no naturales, y su método

Info

Publication number
AR112756A1
AR112756A1 ARP180101920A ARP180101920A AR112756A1 AR 112756 A1 AR112756 A1 AR 112756A1 AR P180101920 A ARP180101920 A AR P180101920A AR P180101920 A ARP180101920 A AR P180101920A AR 112756 A1 AR112756 A1 AR 112756A1
Authority
AR
Argentina
Prior art keywords
mutant
trna
unnatural amino
incorporation
mrna
Prior art date
Application number
ARP180101920A
Other languages
English (en)
Inventor
Floyd E Romesberg
Vivian T Dien
Aaron W Feldman
Emil C Fischer
Yorke Zhang
Hans Aerni
Carolina Caffaro
Jerod Ptacin
Original Assignee
Synthorx Inc
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synthorx Inc, Scripps Research Inst filed Critical Synthorx Inc
Publication of AR112756A1 publication Critical patent/AR112756A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01026—Pyrrolysine-tRNAPyl ligase (6.1.1.26)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

En la presente se divulgan métodos, composiciones y kits para la síntesis de proteínas que comprenden aminoácidos no naturales que utilizan un ARNt mutante. Reivindicación 1: Un método para producir una proteína que contiene un aminoácido no natural; dicho método caracterizado porque comprende: preparar un ARNt mutante, en donde el ARNt mutante comprende una secuencia anticodón mutante seleccionada de la Tabla 1 ó 2; preparar un ARNm mutante, en donde el ARNm mutante comprende una secuencia codón mutante seleccionada de la Tabla 1 ó 2; y sintetizar la proteína que contiene un aminoácido no natural utilizando el ARNt mutante y el ARNm mutante.
ARP180101920A 2017-07-11 2018-07-10 Incorporación de nucleótidos no naturales, y su método AR112756A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201762531325P 2017-07-11 2017-07-11

Publications (1)

Publication Number Publication Date
AR112756A1 true AR112756A1 (es) 2019-12-11

Family

ID=65002471

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP180101920A AR112756A1 (es) 2017-07-11 2018-07-10 Incorporación de nucleótidos no naturales, y su método

Country Status (15)

Country Link
US (4) US20200131555A1 (es)
EP (1) EP3652316A4 (es)
JP (2) JP7325341B2 (es)
KR (2) KR20240038157A (es)
CN (1) CN111051512A (es)
AR (1) AR112756A1 (es)
AU (1) AU2018300069A1 (es)
CA (1) CA3069321A1 (es)
EA (1) EA202090090A1 (es)
IL (1) IL271903A (es)
MA (1) MA49578A (es)
NZ (1) NZ761479A (es)
SG (1) SG11202000167SA (es)
TW (1) TWI821192B (es)
WO (1) WO2019014267A1 (es)

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