WO2007075438A3 - Polypeptides comprising unnatural amino acids, methods for their production and uses therefor - Google Patents

Polypeptides comprising unnatural amino acids, methods for their production and uses therefor Download PDF

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Publication number
WO2007075438A3
WO2007075438A3 PCT/US2006/047949 US2006047949W WO2007075438A3 WO 2007075438 A3 WO2007075438 A3 WO 2007075438A3 US 2006047949 W US2006047949 W US 2006047949W WO 2007075438 A3 WO2007075438 A3 WO 2007075438A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
amino acids
unnatural amino
polypeptides
production
Prior art date
Application number
PCT/US2006/047949
Other languages
French (fr)
Other versions
WO2007075438A2 (en
Inventor
George Church
David Liu
Original Assignee
Codon Devices Inc
George Church
David Liu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codon Devices Inc, George Church, David Liu filed Critical Codon Devices Inc
Publication of WO2007075438A2 publication Critical patent/WO2007075438A2/en
Publication of WO2007075438A3 publication Critical patent/WO2007075438A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Certain aspects of the present invention provide artificial polypeptides containing ; one or more unnatural amino acids and methods for producing and using the artificial polypeptides.
PCT/US2006/047949 2005-12-15 2006-12-15 Polypeptides comprising unnatural amino acids, methods for their production and uses therefor WO2007075438A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US75139705P 2005-12-15 2005-12-15
US60/751,397 2005-12-15

Publications (2)

Publication Number Publication Date
WO2007075438A2 WO2007075438A2 (en) 2007-07-05
WO2007075438A3 true WO2007075438A3 (en) 2007-10-18

Family

ID=38050881

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/047949 WO2007075438A2 (en) 2005-12-15 2006-12-15 Polypeptides comprising unnatural amino acids, methods for their production and uses therefor

Country Status (1)

Country Link
WO (1) WO2007075438A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9023601B2 (en) 2002-09-12 2015-05-05 Gen9, Inc. Microarray synthesis and assembly of gene-length polynucleotides
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US20100297698A1 (en) * 2007-10-08 2010-11-25 Radha Krishnakumar System and method for producing synthetic microorganisms capable of translating proteins containing non-standard amino acids
BRPI0906388A2 (en) * 2008-02-08 2015-07-07 Scripps Research Inst Immune tolerance breakdown with a genetically encoded unnatural amino acid
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
EP4039363A1 (en) 2010-11-12 2022-08-10 Gen9, Inc. Protein arrays and methods of using and making the same
EP3594340B1 (en) 2011-08-26 2021-06-30 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP3543350B1 (en) 2012-04-24 2021-11-10 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2014004393A1 (en) 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
CN109844105A (en) 2016-07-11 2019-06-04 得克萨斯州大学系统董事会 Recombinant polypeptide and its production method comprising selenocysteine
CA3034701A1 (en) * 2016-08-30 2018-03-08 Board Of Regents, The University Of Texas System Production of seleno-biologics in genomically recoded organisms
CN112553239B (en) * 2019-09-25 2023-10-13 深圳华大生命科学研究院 Genome rearrangement control system and method based on unnatural amino acid
WO2022235825A1 (en) * 2021-05-04 2022-11-10 Opentrons LabWorks Inc. Methods and compositions for controlling release factor activity and uses thereof
WO2023070114A2 (en) * 2021-10-22 2023-04-27 Terremoto Biosciences, Inc. Reversible lysine covalent modifiers of egfr and uses thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHIN J W ET AL: "An Expanded Eukaryotic Genetic Code", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 301, 15 August 2003 (2003-08-15), pages 964 - 967, XP002996001, ISSN: 0036-8075 *
LIU WENSHE ET AL: "Genetic incorporation of unnatural amino acids into proteins in mammalian cells.", NATURE METHODS MAR 2007, vol. 4, no. 3, March 2007 (2007-03-01), pages 239 - 244, XP009084564, ISSN: 1548-7091 *
SAKAMOTO K ET AL: "Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 30, no. 21, 1 November 2002 (2002-11-01), pages 4692 - 4699, XP002976283, ISSN: 0305-1048 *
XIE J ET AL: "An expanding genetic code", METHODS : A COMPANION TO METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC., NEW YORK, NY, US, vol. 36, no. 3, July 2005 (2005-07-01), pages 227 - 238, XP004997375, ISSN: 1046-2023 *
ZHANG ZHIWEN ET AL: "Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 101, no. 24, 15 June 2004 (2004-06-15), pages 8882 - 8887, XP002435941, ISSN: 0027-8424 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9023601B2 (en) 2002-09-12 2015-05-05 Gen9, Inc. Microarray synthesis and assembly of gene-length polynucleotides
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis

Also Published As

Publication number Publication date
WO2007075438A2 (en) 2007-07-05

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