WO2018205691A1

WO2018205691A1 - System for processing histopathological specimen

Info

Publication number: WO2018205691A1
Application number: PCT/CN2018/074373
Authority: WO
Inventors: 张磊; 曾斌; 吴汉环; 李娟�
Original assignee: 南昌德漫多科技有限公司
Priority date: 2017-05-09
Filing date: 2018-01-27
Publication date: 2018-11-15
Also published as: CN106979878A; CN106979878B

Abstract

Disclosed is a system for processing a histopathological specimen, which system belongs to the technical field of medical devices. The system for processing a histopathological specimen comprises a movable clamping unit, a dehydration and wax immersion unit (2-1), a slicing unit (3-1), a slide glass dyeing unit (4-1), an image scanning and display unit and a main control unit. By using each of the units above, the series of processing steps, involving fixing, dehydrating, embedding, slicing, rinsing, dyeing and photographing a histopathological specimen after taking materials, can be automatically completed, thereby reducing the workload of skilled persons, preventing the skilled persons from coming into contact with toxic and harmful substances, and shortening the processing time of histopathological specimens.

Description

Histopathological specimen processing system

Technical field

The invention relates to the technical field of medical instruments, in particular to a tissue pathological specimen processing system.

Background technique

The prerequisite for accurate pathological diagnosis is scientific and standardized treatment of histopathological specimens (hereinafter referred to as specimens), and the production of qualified pathological sections is provided to the pathologist. The processing of specimens includes several major steps such as taking, fixing, dehydrating, embedding, slicing, bleaching, dyeing, and sealing. Among them, staining can be divided into hematoxylin and eosin staining, special enzyme histochemical staining, immunohistochemical staining, in situ hybridization and the like. The implementation of these steps requires the use of a variety of volatile toxic agents that have varying degrees of hazard to the health of the technician. This requires a highly automated device to reduce manual operations and thus protect the health of the technician.

Pathological diagnosis needs to be accurate, and timely, and the sooner the pathological diagnosis is obtained, the more favorable it is for the diagnosis and treatment of the patient's disease. The traditional specimen processing process needs to go through 3-5 days or even longer. The reason is that on the one hand, each step of the specimen has a clear processing time requirement, and insufficient time will affect the quality of the slice, thus affecting the diagnosis; The aspect is that each step is completed until the next step, because the technician's work handover, rest and other factors waste time. Therefore, a highly automated device is needed to process the specimen, and the time required for the connection between the different steps is saved under the premise that the processing time of each step is sufficient, the artificial time waste is avoided, and the time required for the processing of the specimen is shortened, thereby Let patients get a pathological diagnosis earlier.

After the specimen has undergone all the processing and is made into a qualified tissue section, it will be read by the pathologist and a diagnosis will be made. If other pathologists are required for remote consultation, the tissue slices should be manually placed on a digital pathology scanner for image acquisition to obtain a digitized image for remote transmission. If the direct scan becomes a digital image after the slice is created, it will save manual work and save time.

At present, a large number of automatic dewatering machines, automatic dyeing machines, automatic film sealers and fully automatic digital pathology scanners have been put into practical work, but these devices only replace the staff to complete one of the steps, and can not be continuous. All the steps are completed, and manual steps are still required between the different steps, and some steps can only be performed manually, such as slicing and drifting.

If the whole process of specimen fixation, dehydration, embedding, slicing, bleaching, staining and image acquisition can be realized, it can meet the requirements of simpler, more reliable and more efficient specimen processing.

Summary of the invention

In order to solve the above technical problems, the present invention provides a histopathological specimen processing system capable of automatically completing a tissue specimen processing flow in sequence.

The technical solution adopted by the invention is:

A histopathological specimen processing system, comprising

a movable clamping unit for gripping the specimen and moving the tissue specimen or the carrier on which the specimen is placed, comprising a robot and a guide rail for moving the robot, the end of the robot being a rotating member and being mounted with a jaw;

- dehydration dipping wax unit for washing, dehydrating, transparent, dipping wax and embedding the specimen, realizing the specimen into the wax block specimen box and making into a wax block, including a dehydrating waxing mechanism and a dehydrating liquid cylinder group. a wax supply mechanism, a third pipette and a first temperature adjustment mechanism; the dewatering and waxing mechanism is provided with a plurality of independent cavities, and a plurality of wax block specimen boxes are installed in the cavity; the dehydration cylinder group For storing the sample dehydration liquid and providing the dehydration waxing mechanism for storing the liquid paraffin and supplying the dehydration waxing mechanism, and the third pipette for transferring the liquid for dehydration of the tissue specimen; the temperature control The mechanism is used to regulate the melting and solidification of paraffin;

- a slicing unit for slicing and rinsing a specimen embedded in a wax specimen box, obtaining a slice from an embedded specimen in the wax block, including a slicer body, a wax block specimen box clamping mechanism, and a sectioning tank a mechanism, a first moving mechanism and a second moving mechanism; the middle portion of the slicer body protrudes forwardly to form a boss, and a first moving mechanism for longitudinally reciprocating movement of the boss is mounted, and the surface of the boss is mounted a wax block clamping mechanism; the base of the slicer body extends forwardly below the above-mentioned boss to form a slicing platform, and the slicing platform is provided with a slicing groove mechanism, and a slicing groove is also installed between the slicing groove mechanism and the surface of the platform. a second moving mechanism that reciprocates laterally of the mechanism;

a slide dyeing unit for baking, dewaxing, and dyeing the tissue specimen slides that have been sliced in the above-described slicing unit, including a first staining group and a second staining group; the first staining group including the first staining group a chamber, a second temperature adjustment mechanism, a first dyeing cylinder group and a first pipette; the second dyeing group comprises a second dyeing chamber, a second dyeing cylinder group and a second pipette;

An image scanning display unit for collecting an image of the pathological slice;

a main controller unit for controlling and organizing the operation of the electrical devices in the above units and for connecting to the external control collection terminal, including the main control chip and the external connection port.

The robot, the guide rail and the jaw in the movable clamping unit are formed as a six-axis robot.

The jaws of the six-axis robot are also provided with an identification reader.

The wax block sample box comprises a box body and a specimen carrier groove, wherein the sample carrier groove is outwardly convex, and is composed of an outer groove of the separable sample carrier and an inner groove of the sample carrier, and the groove faces of the two are matched. A fine through hole is opened, and the tissue specimen is placed between the outer groove of the sample carrier and the inner groove of the sample carrier, and the to-be-cut surface is attached to the outer groove of the sample carrier.

In the cauterization cavity of the dehydration wax-discharging mechanism, a wax block specimen box carrier strip is sequentially arranged in the axial direction, and the wax block specimen box is sequentially mounted on the surface of the wax block specimen box carrier strip.

The wax block specimen box carrying strip is provided with a front connecting end and a rear connecting end, respectively, which are respectively rotatably connected with the front frame and the rear frame of the block cavity, and the front connecting end protrudes outwardly from the frame to form a rotating shaft The rotating shaft is equipped with a gear, the gears of the adjacent wax block specimen box carrier meshes with each other, and a stepping motor for driving the gear rotation is installed at the gear of the wax block specimen box carrier bar located at the first position.

The wax feeding mechanism comprises: an air compressor, an electric control valve, a wax storage tank and a wax conveying tube, wherein the wax conveying tube communicates with the cauterization cavity and the wax storage tank of the dehydration and waxing mechanism, and the liquid paraffin is in the block Flow between the chamber and the storage tank.

The wax block clamping mechanism comprises a clamping opening and a push rod, and an opening is arranged on one side of the clamping opening, and the wax block specimen box is inserted into the clamping opening through the opening, the clamping mouth and the wax block sample box The longitudinal direction is an interference fit, and the clamping opening is laterally matched with the wax block specimen box; the other side of the clamping opening is a closed frame, and a push rod is connected to the outer side of the closed frame, and the closed frame is The push rods are connected by an elastomer.

The grooving mechanism has a groove surface that is inclined downward, and further includes

a blade device, the front surface of the front end of the dicing groove mechanism is laterally disposed with a blade and the blade edge is upward, and the front end of the dicing groove mechanism is disposed at a sipe for loading the blade;

- a left slice chamber, the left slice chamber is provided with a separable sliced piece drawer;

a right slice cavity separated from the left slice cavity by a partition and independent of each other, wherein the right slice cavity is divided into an upper cavity and a lower cavity and separated by a partition, and a slide table is mounted on the left side of the upper cavity. a slide clip is disposed on the right side of the upper chamber, the slide clip is in the shape of a cavity of the inner interlayer, and a scanning window for dip dyeing is disposed on a front surface thereof, and a slit for entering the slide is disposed on a side surface thereof; a nip of the slide holder corresponding to a lateral position of the slide surface of the slide table; a left side of the slide table is provided with a slide squeegee for pushing the slide piece carrying the tissue slice to the right to the load In the clip. a liquid outflow device comprising a first outflow port and a second outflow port, each controlled by a solenoid valve, the first outflow port being embedded in a partition between the upper chamber and the lower chamber for drawing liquid from the upper chamber into the lower chamber, a second outlet is provided on the bottom side of the lower chamber for discharging the liquid in the lower chamber;

- an intracavity liquid circulation device comprising an inlet pipe, a filter, a water pump and an outlet pipe, the inlet pipe mouth being provided in the lower chamber for extracting liquid in the lower chamber by a water pump, the filter being used for The liquid entering the liquid inlet tube is inserted into the partition between the upper chamber and the lower chamber for discharging the liquid extracted in the lower chamber into the upper chamber through the water pump;

a third temperature adjustment mechanism for heating the liquid in the upper chamber;

a slice detecting device comprising a detecting port disposed above the slicing mechanism.

The upper end and the lower end of the slide squeegee are fixed ends, and the upper end and the lower end are symmetrically connected with a sliding piece, and the sliding piece reciprocates laterally through the slide rail of the bottom; the bottom of the slide table is further provided with a slide piece for the slide piece, After the top slide is pushed open, a new slide is bounced; a press wheel is arranged above the slide table, and the bottom of the press wheel is attached to the slide for avoiding when the slide scraper pushes the slide The slide moves perpendicular to the slide.

The sipe is an electromagnet sipe.

a dye bath is regularly disposed in the first dyeing chamber, and the carrier clip is inserted into the dyeing tank, and the second temperature adjusting mechanism includes a heating body for adjusting the liquid temperature and the baking sheet in the first dyeing chamber. .

A slide holder mounting strip is disposed in the second dyeing chamber, and the slide clip mounting strip is sequentially provided with a plurality of slide clip mounting ports.

The image scanning display unit includes a scanning mechanism and an image display.

The first pipette, the second pipette or the third pipette includes a pipette and a pipetting guide, and the pipetting guide is composed of an X-axis pipetting guide and a Y-axis pipetting guide.

The histopathological specimen processing system of the invention can automatically complete the tissue specimen processing flow in turn, and the advantages thereof are as follows:

1. The series of processing steps of fixing, dehydrating, embedding, slicing, bleaching, dyeing and image collecting of the histopathological specimen after taking the material can be automatically completed, and the present invention omits the step of sealing the sheet, directly inserting the clip Scanning to obtain digital images helps save time;

2, fully automated operation, to avoid contact with toxic and harmful reagents;

3, the entire process standardization, to avoid the differences between different slice experiments, improve the quality of histopathological sections;

4. The processes are closely linked to shorten the processing time of histopathological specimens;

5. The digital image of the pathological slice obtained finally is scanned and transmitted instantly, which is convenient for remote consultation.

Instruction sheet

Figure 1 is a schematic view showing the overall structure of the system of the present invention;

2 is a schematic structural view of a six-axis robot;

Figure 3 is a schematic structural view of a dehydration waxing mechanism;

Figure 4 is a schematic structural view of a wax block specimen box;

Figure 5 is a schematic structural view of a wax supply mechanism;

6 is a schematic view showing the overall structure of a slicing unit;

Figure 7 is a schematic structural view of a wax block specimen box clamping mechanism;

Figure 8 is a schematic structural view of a slicing mechanism;

Figure 9 is a schematic view showing the structure of the liquid circulation in the right slice chamber;

Figure 10 is a schematic view showing the structure of the first dyeing chamber;

Figure 11 is a schematic view showing the structure of the second dyeing chamber;

Figure 12 is a schematic view showing the structure of the first (second, third) pipette;

Figure 13 is a connection diagram of the main controller unit.

Illustration:

In Fig. 1, a 1-1 six-axis robot, a 2-1 dehydrated wax unit, a 3-l slicing unit, a 4-l slide dyeing unit, a 5-l scanning mechanism, a 5-2 display, and a 6-1 first shift Liquid device, 6-2 second pipette, 6-3 third pipette, 7-1 dewatering cylinder group, 7-2 first dyeing liquid cylinder group, 7-3 second dyeing liquid cylinder group;

In Figure 2, 1-1 six-axis robot, l-2 jaws, 1-3 identification reader;

In Fig. 3, 2-2 dehydration waxing mechanism, 2-3 block cavity, 2-4 wax block specimen box carrier, 2-5 gear, 2-6 stepping motor, 2-7 wax block specimen box;

In Fig. 4, 2-7 wax block specimen box, 2-8 box body, 2-9 specimen carrier trough outer groove, 2-10 specimen carrier trough inner groove;

In Figure 5, 2-11 air compressor, 2-12 electric control valve, 2-13 storage wax tank, 2-14 wax tube;

In Fig. 6, the 3-1 slicing unit, the 3-2 slicing machine base, the 3-3 wax block specimen box clamping mechanism, the 3-4 first moving mechanism, the 3-5 slicing mechanism, and the 3-6 second movement Institution, 3-27 slice detection device;

In Fig. 7, 3-3 wax block specimen box clamping mechanism, 3-7 clamping mouth, 3-8 push rod, 3-9 spring;

In Figure 8, 3-5 slice slot mechanism, 3-10 blade, 3-11 electromagnet slot, 3-12 left slice cavity, 3-13 sliced block drawer, 3-14 right slice cavity, 3-15 Cavity, 3-16 lower chamber, 3-17 slide table, 3-18 slide holder, 3-19 slide scraper, 3-20 pressure roller;

In Figure 9, 3-21 first outflow port, 3-22 second outflow port, 3-23 inlet pipe, 3-24 filter, 3-25 water pump, 3-26 outlet pipe;

In Fig. 10, 4-2 first dyeing chamber, 4-3 dyeing tank, 3-18 slide holder;

In Fig. 11, 4-4 slide clip mounting strip, 4-5 slide clip mounting port, 3-18 slide clip;

In Figure 12, the 6-4 pipette, 6-5X axis pipetting guide, 6-6Y axis pipetting guide.

detailed description

The invention will be further described below in conjunction with the accompanying drawings.

Referring to Figure 1, Figure 1 of the present invention provides a histopathological specimen processing system including a movable clamping unit, a dehydrated waxing unit 2-1, a slicing unit 3-1, a slide dyeing unit 4-1, and an image scanning. Acquisition unit and main control unit. The movable clamping unit is used for clamping the specimen and the moving tissue specimen or the carrier with the specimen, and the dehydrated waxing unit 2-1 is used for washing, dehydrating, transparent, dipping and embedding the specimen, thereby loading the specimen into the specimen. The wax block specimen box 2-7 was made into a wax block. The slicing unit 3-1 is for slicing and rinsing the specimen embedded in the wax specimen box 2-7 to obtain a slice from the embedded specimen in the wax block. The slide dyeing unit 4-1 performs baking, dewaxing, and dyeing of the tissue sample slides which have been sliced in the above-described slicing unit 3-1. The image scanning acquisition unit is used to acquire an image of the stained tissue section. The main control unit is used for controlling and organizing the operation of the electrical devices in the above units, and connecting with the external control collection terminal, including the main control chip and the external connection port, and the control connection relationship between the control chip and other units is as shown in FIG. Shown.

As shown in FIG. 1 and FIG. 2, the movable clamping unit of the unit is clamped and transferred by a six-axis robot 1-1 for slicing, carrier clips 3-18, etc., and the six-axis robot ll can be extended to The various locations in the processing system shown in Figure 1. At the same time, in the process of dehydrating, dipping, and embedding the tissue specimen, the wax block specimen box 2-7 is marked with an identification code, and therefore, the claw portion of the six-axis robot 1-1 is also provided with a logo. The reader 1-3 is used for reading information such as identifying the source of each tissue specimen, and is convenient for recording and tracking information.

The dehydration dipping wax unit is composed of a dehydration dip wax mechanism 2-2, a dehydration liquid cylinder group 7-1, a wax supply mechanism, a third pipette 6-3, and a first temperature adjustment mechanism. As shown in Fig. 3, the dehydration waxing mechanism 2-2, in the embodiment, the dehydration and waxing mechanism 2-2 is provided with three independent cavities 2-3, and the adjacent cavities 2-3 pass through the partitions. Separated. In each of the cavities 2-3, the wax block specimen box carrier strips 2-4 are sequentially arranged in the axial direction, and the wax block specimen box carrier strips 2-4 are sequentially provided with depressions for the wax-filled specimen box 2-7. The shape and size of the recess coincide with the outer slots 2-9 of the specimen carrier. The wax block specimen box carrier strip 2-4 is provided with a front connecting end and a rear connecting end, and the two are respectively rotatably connected with the front frame and the rear frame of the block cavity 2-3, and the front connecting end protrudes out of the frame Forming a rotating shaft, the rotating shaft is equipped with gears 2-5, the gears 2-5 of the adjacent wax block specimen box carrying strips 2-4 are meshed with each other, and the gears 2-5 of the wax block specimen box carrying strips 2-4 located at the first position A stepping motor 2-6 for driving the gear 2-5 is mounted.

The wax block sample box 2-7 shown in FIG. 4 includes a box body 2-8 and a specimen carrier, and the wax block sample box 2-7 includes a box body 2-8 and a specimen carrier groove, and the specimen carrier groove is convex outward. The detachable specimen carrier outer groove 2-9 and the sample carrier inner groove 2-10 are formed and matched with each other, and the groove faces of both of them are provided with fine through holes, and the tissue specimen is placed on the outer groove of the sample carrier. 2-9 and the groove 2-10 between the specimen carrier, and the surface to be cut is attached to the outer groove of the specimen carrier. The wax block specimen box 2-7 is made of plastic, and does not undergo significant deformation after contacting the reagent of the specimen, and the specimen carrier portion can be easily cut by the blade 3-10 of the microtome; after the specimen is placed, the specimen contains The grooves 2-10 in the groove recess the compliance inward to accommodate the specimen.

The dehydration liquid cylinder group 7-1 is composed of a plurality of ported containers containing the tissue sample dehydration liquid, and is arranged in order on the dehydration and waxing mechanism 2-2 side, when the tissue specimen is subjected to dehydration and transparency steps. The corresponding dehydrating liquid is sucked from the container by the third pipette 6-3 and transferred to the cauterization chamber 2-3 of the dehydration dipping wax mechanism 2-2 to submerge the respective wax block specimen boxes 2-7.

The wax supply mechanism shown in FIG. 5 includes an air compressor 2-11, an electric control valve 2-12, and a wax conveying tube 2-14, and a wax tube 2-14 is connected to the caulking chamber of the dehydrating and waxing mechanism 2-2. Body 2-3 and storage tank 2-13, liquid paraffin flows between the cavities 2-3 and the storage tanks 2-13. After the liquid paraffin enters the block cavity 2-3 and is immersed in the wax block specimen box carrier strip 2-4, the wax block sample box carrier strip 2-4 is rotated to the horizontal position, so that the block cavity 2-3 can be made After most of the paraffin wax returns to the storage tank 2-13, some paraffin remains in the depression of the wax block specimen box carrier 2-4. When the temperature is lowered below the melting point of the paraffin wax, the paraffin wax in the depression solidifies. To make a wax block. The paraffin is recycled, and when the paraffin in the storage tank 2-13 is insufficient, the artificial addition is timely.

In the dehydration and transparency stage of the tissue specimen, when the wax block specimen box carrier 2-4 is in the lateral tilt position, the depression corresponding to the installation wax block specimen box 2-7 does not leave liquid, that is, each liquid acts on the tissue specimen. After a certain period of time, it needs to be completely removed before the next liquid can be added. The wax block specimen box carrier strips 2-4 are inclined, and the sample carrier tank of the wax block specimen box 2-7 does not leave a added dehydration liquid. . When entering the waxing and embedding stage, the wax block specimen box carrier strip 2-4 is placed in a horizontal horizontal position, and the liquid corresponding to the volume thereof can be retained in the depression. At this time, the paraffin wax is added to the wax specimen box through the wax supply mechanism. In the specimen carrier of 2-7, the paraffin wax other than the depression is caused to flow away from the cavities 2-3 to the storage tank 2-13, and the temperature of the cauterization chamber 2-3 is lowered by the first temperature adjustment mechanism. The wax is solidified to successfully form a wax block containing the tissue specimen, and then the molded wax block is held by the six-axis robot 1-1 and transferred to the slicing unit 3-1.

The slicing unit 3-1 shown in Figs. 1 and 6 is composed of a slicing machine body 3-2, a wax block specimen box chucking mechanism 3-3, a slicing tank mechanism 3-5, a first moving mechanism 3-4, and The second moving mechanism 3-6 is composed. As shown in FIG. 7, the middle section of the slicer base 3-2 protrudes forward to form a boss, and the first moving mechanism 3-4 is a longitudinal limitable slider and a sliding surface, and is mounted on the boss and the slicer base. The joint of the body 3-2 allows the boss to reciprocate longitudinally. The surface of the boss is mounted with a wax block clamping mechanism, and the wax block clamping mechanism includes a clamping opening 3-7 and a push rod 3-8, and an opening is provided on one side of the clamping opening 3-7. In operation, the wax block specimen box 2-7 is moved from the block cavity 2-3 to the opening insertion accommodating port 3-7 by the six-axis robot ll, the spigot port 3-7 and the wax block sample box 2 - 7 The longitudinal direction is an interference fit, and the clamping opening 3-7 is laterally matched with the wax block specimen box 2-7, which can make the wax block sample box 2-7 not move when slicing up and down, and can also facilitate the wax. The specimen box 2-7 is horizontally accessed. The other side of the clamping opening 3-7 is a closed frame, and a push rod 3-8 is connected to the outer side of the closed frame, and the closed frame and the push rod 3-8 are connected by a pair of symmetrical springs 3-9, in operation When replacing the wax block specimen box 2-7, the six-axis robot 1-1 taps the push rod 3-8, and the wax block specimen box 2-7 can be ejected from the wax block clamping mechanism, the six-axis robot 1 The -1 block can be easily removed.

The bottom of the slicer base 3-2 extends forwardly below the above-mentioned boss to form a slicing platform. The slicing platform is provided with a slicing mechanism 3-5, and the second moving mechanism 3-6 is a laterally constrainable slider and sliding. The surface is mounted at the joint of the slicing platform and the slicing mechanism 3-5 so that the slicing mechanism 3-5 can be reciprocally moved laterally. As shown in Fig. 8, the slicing groove mechanism 3-5 has a groove surface which is slightly inclined downward. The mechanism includes a blade 3-10 replacing device, a left slicing chamber 3-12, a right slicing chamber 3-14, a liquid outflow device, Intracavity liquid circulation device, third temperature adjustment mechanism and slice detection device 3-27. The front surface of the front end of the slicing groove mechanism 3-5 is laterally disposed with the blade 3-10 and the cutting edge faces upward (the corresponding one of the left slicing chamber and the right slicing chamber), and the front end of the slicing slot mechanism 3-5 is disposed on the blade 3- 10 Electromagnet sipe 3-11. When the blade 3-10 is replaced, the electromagnet slot 3-11 is de-energized, the old blade 3-10 is taken out by the six-axis robot 1-1, and the new blade 3-10 is placed in the electromagnet slot 3- 11; during operation, the electromagnet sipe 3-11 is energized to suck the blade 3-10 to perform the slicing work. The electromagnet is de-energized, the robot removes the blade of the left slicing chamber, and the blade of the right slicing chamber is placed in the slot of the left slicing chamber. The six-axis robot removes the new blade from the new blade storage box and installs the knife in the right slicing chamber. In the slot, at this time, the electromagnet is energized to suck and install the two blades that are replaced. Since the left slice cavity is used to rough the wax block, the blade is not required, and the right slice cavity is used to obtain the tissue slice. The blade is required to be high and must be sharp and free of defects. Therefore, when the blade is replaced, the right slice cavity is The blade is placed in the left slice cavity for reuse, which saves the blade.

The left slice chamber 3-12 and the right slice chamber 3-14 are separated by a partition and are independent of each other. After the wax block specimen box 2-7 is placed in the dicing slot mechanism 3-5, the specimen in the wax block specimen box 2-7 needs to be trimmed before being formally sliced, that is, the wax block specimen box 2-7 The convex outer layer is cut and cut until the tissue surface of the tissue specimen is complete. Therefore, the left slice chamber 3-12 is provided with a separable and replaceable sliced fragment drawer 3-13, and the specimen in the wax block specimen box 2-7 is trimmed. When the cut pieces are directly dropped into the sliced pieces drawers 3-13, the sliced pieces of drawers 3-13 can be cleaned or replaced during cleaning, and the cut pieces are not scattered and scattered, and it is difficult to clean them up. . The right slice chamber 3-14 is divided into an upper chamber 3-15 and a lower chamber 3-16 and separated by a partition. A slide table 3-17 is mounted on the left side of the upper chamber 3-15, and a slide clip 3-18 is disposed on the right side of the upper chamber 3-15. The slide clip is a cavity shape of the inner sandwich, and the front surface thereof is provided. There are scanning windows for dip dyeing and image acquisition. The carrier clips 3-18 are made of transparent plastic material or glass material to avoid corrosion, deformation, etc., which may be caused by the action of the dyeing liquid when entering the dyeing step. The scanning windows of the sheets 3-18 are placed toward the bottom of the upper chamber, and the sides thereof are provided with the slits for the slides to enter, and the jaws of the slide holders 3-18 correspond to the lateral positions of the surface slides of the slide table 3-17. The left side of the slide table 3-17 is provided with a slide scraper 3-19, and the upper end and the lower end of the slide scraper 3-19 are fixed ends, and the upper end and the lower end are symmetrically connected with a slide piece, and the slide piece passes through the slide rail at the bottom. Transverse reciprocating movement for pushing the slide with the tissue slice to the right into the slide holder 3-18; above the slide table 3-17, there is a pressure roller 3-20, which is 3- 20 consists of 4 rubber wheels and is divided into four corners of the slide. The bottom of each rubber wheel is attached to the surface of the slide. It is used to prevent the slide from moving perpendicular to the slide when the slide scraper 3-19 pushes the slide. There is also a slide shrapnel on the bottom of the slide table 3-17 for popping up a new slide after the top slide is pushed open.

As shown in FIG. 9, the liquid outflow device includes a first outflow port 3-21 and a second outflow port 3-22, both of which are controlled by a solenoid valve, and the first outflow port 3-21 is embedded in the upper chamber 3-15 and the lower chamber 3-16. Among the partitions, the liquid for the upper chamber 3-15 is pumped into the lower chamber 3-16, and the second outlet port 3-22 is provided at the bottom side of the lower chamber 3-16 for the lower chamber 3-16. The liquid is released. The intracavity liquid circulation device comprises a liquid inlet tube 3-23, a filter 3-24, a water pump 3-25 and a liquid outlet tube 3-26, and the inlet tube 3-23 nozzle is disposed in the lower chamber 3-16 for The liquid in the lower chamber 3-16 is pumped through the water pump 3-25, and the filter 3-24 is used to filter the liquid entering the inlet tube 3-23, and the outlet tube 3-26 is embedded in the upper chamber 3-15 and below. The partitions between the chambers 3-16 serve to flow the liquid extracted in the lower chambers 3-16 through the water pumps 3-25 into the upper chambers 3-15. The heating body of the third temperature adjustment mechanism is disposed in the upper chamber 3-15. Used to heat the liquid in the upper chamber 3-15. The slice detecting device 3-27 is configured to detect and determine whether the sliced flake has reached the requirement. The imaging device has a detecting port and a camera disposed above the slicing mechanism 3-5, and the slice screen is captured and transmitted for analysis.

When the slice is formally sliced, the slice slot is moved to the left, so that the right slice slot is located directly below the wax block clamping mechanism, and the upper cavity 3-15 of the right slice cavity 3-14 is filled with water (the upper cavity 3 is passed through the third temperature adjustment mechanism) After the -15 water temperature is controlled at 45 ° C), the liquid surface is immersed in the portion of the blade 3-10 which is less than 1 mm. When the tissue section of the wax block specimen box 2-7 is cut, it will float directly into the water, and the sliced tissue sections will be connected end to end, and the end of the last cut tissue section is connected to the blade. The cut tissue section is monitored by the detecting device. When the wrinkles of the tissue section disappear, that is, the first outflow port 3-21 is controlled to slowly lower the water surface of the upper chamber 3-15, and the tissue section will not flow along the water. It is attached to the slide down, and then the slide slide 3-19 is pushed by the six-axis robot 1-l to push the slide with the tissue slice to the right, and inserted into the slide clip 3-18 to control the six-axis robot 1- 1 The carrier clip 3-18 is taken out and transferred to the slide dyeing unit 4-1, and the new slide holder 3-18 is replaced by the six-axis robot 1-1. At this time, the water transferred to the lower chamber 3-16 is transferred to the inlet pipe 3-23 by the water pump 3-25 through the intracavity liquid circulation device, filtered through the filter 3-24, and then re-circulated through the outlet pipe 3-26. Return to the upper chamber 3-15, at this time, replace the new slide with the slide shrapnel, and repeat the above slicing work.

The slide dyeing unit 4-1 is composed of a first staining group and a second staining group, wherein the first staining group is a staining group used for performing a conventional staining step, and the second staining group is subjected to special staining (such as special enzyme histochemical staining). The staining group used in the steps of immunohistochemical staining, in situ hybridization, etc.

The first staining group shown in FIG. 10 includes a first staining chamber 4-2, a second temperature adjusting mechanism, a first staining cylinder group 7-2, and a first pipette 6-1. The first dyeing chamber 4-2 is regularly provided with a plurality of dyeing tanks 4-3, the carrier clips 3-18 are inserted into the dyeing tank, and the second temperature adjusting mechanism comprises a heating body for adjusting the first dyeing chamber 4 - 2 liquid temperature and baking sheet, the heating body is a resistance wire heated aluminum sheet provided in the first dyeing chamber 4-2. The carrier clips 3-18 are picked up by the six-axis robot 1-1 and inserted into different dye baths 4-3, and the dyeing liquid is transferred from the first pipette set 7-1 from the first dyeing tank group 7-2. The corresponding dyeing liquid is sucked and sent to the carrier clip 3-18 of the corresponding dyeing tank 4-3, and after the delivery is completed, the used staining liquid is sucked back through the first pipette 6-1 and transported to the first The original portion of the dyeing liquid cylinder group 7-2 was reused, and the dyeing was sequentially performed in accordance with this method.

The second staining group shown in Fig. 11 includes a second staining chamber, a second staining cylinder group 7-3, and a second pipette 6-2. As shown in FIG. 12, the second dyeing chamber is provided with a slide holder mounting strip 4-4, and the slide holder mounting strip 4-4 is sequentially provided with a plurality of slide holder mounting ports 4-5, since the second dyeing group is For special dyeing, the frequency of use is generally lower than that of the first dyeing group, so the carrier clip 3-18 of the slide holder mounting strip 4-4 can be loaded in a smaller number than the carrier clip in the first dyeing group 3- The number of 18s that can be loaded. When the second dyeing group performs the dyeing, the liquid added to the slide holder for the tissue treatment is no longer returned to the dyeing liquid cylinder group 7-3, but is completed by the vacuum suction hole provided on the slide holder mounting strip 4-4. The liquid after the dyeing step is aspirated and flows to the waste tank.

Figure 12 is a schematic view showing the structure of the first pipette 6-1, the second pipette 6-2, and the third pipette 6-3, both of which are composed of a pipetting 6-4 and a pipetting guide, wherein pipetting The guide rail is composed of an X-axis pipetting guide 6-5 and a Y-axis pipetting guide 6-6, so that the pipetting gun 6-4 can be moved to any position of the unit to pick up and discharge the relevant liquid.

As shown in FIG. 1, the image scanning display unit includes a scanning mechanism 5-1 and an image display 5-2, and the scanning mechanism 5-1 is provided with a carrier clip 3-18 delivery port. After the dyed carrier clip 3-18 is completed, the cedar oil can be added in the last step, the sealing step in the conventional step can be omitted, and the carrier clip 3-18 can be directly scanned to obtain a corresponding image, and the image display 5 is obtained. Displayed in -2.

The above description is only a preferred embodiment of the present invention, and the description thereof is more specific and detailed, but is not to be construed as limiting the scope of the invention. It should be noted that various modifications, improvements and substitutions may be made by those skilled in the art without departing from the spirit and scope of the invention. Therefore, the scope of the invention should be determined by the appended claims.

Claims

A histopathological specimen processing system, characterized in that:

a movable clamping unit for gripping the specimen and moving the tissue specimen or the carrier on which the specimen is placed, comprising a robot and a guide rail for moving the robot, the end of the robot being a rotating member and being mounted with a jaw;

- Dehydrated waxing unit for washing, dehydrating, transparent, dipping wax and embedding the specimen, and inserting the specimen into the wax block specimen box and making it into a wax block, including a dehydrating waxing mechanism, a dehydrating liquid cylinder group, a wax supply mechanism, a third pipette and a first temperature adjustment mechanism; the dewatering and waxing mechanism is provided with a plurality of independent cavities, and a plurality of wax block specimen boxes are installed in the cavity; the dehydration cylinder group For storing the sample dehydration liquid and providing the dehydration waxing mechanism for storing the liquid paraffin and supplying the dehydration waxing mechanism, and the third pipette for transferring the liquid for dehydration of the tissue specimen; the temperature control The mechanism is used to regulate the melting and solidification of paraffin;

- a slicing unit for slicing and rinsing the specimen embedded in the wax specimen box, obtaining a slice from the specimen embedded in the wax block, including a slicing machine body, a wax block specimen box clamping mechanism, and a sectioning tank a mechanism, a first moving mechanism and a second moving mechanism; the middle portion of the slicer body protrudes forwardly to form a boss, and a first moving mechanism for longitudinally reciprocating movement of the boss is mounted, and the surface of the boss is mounted a wax block clamping mechanism; the base of the slicer body extends forwardly below the above-mentioned boss to form a slicing platform, and the slicing platform is provided with a slicing groove mechanism, and a slicing groove is also installed between the slicing groove mechanism and the surface of the platform. a second moving mechanism that reciprocates laterally of the mechanism;

a slide dyeing unit for baking, dewaxing, and dyeing the tissue specimen slides that have been sliced in the above-described slicing unit, including a first staining group and a second staining group; the first staining group including the first staining group a chamber, a second temperature adjustment mechanism, a first dyeing cylinder group and a first pipette; the second dyeing group comprises a second dyeing chamber, a second dyeing cylinder group and a second pipette;

An image scanning display unit for collecting an image of the pathological slice;

a main controller unit for controlling and organizing the operation of the electrical devices in the above units and for connecting to the external control collection terminal, including the main control chip and the external connection port.
A histopathological specimen processing system according to claim 1, wherein the robot, the guide rail and the jaw in the movable grip unit are formed as a six-axis robot.
A histopathological specimen processing system according to claim 2, wherein the jaws of the six-axis robot are further provided with an identification reader.
The histopathological specimen processing system according to claim 1, wherein the wax block specimen box comprises a box body and a specimen carrier, the specimen carrier groove is outwardly convex, and the separable specimen carrier is provided. The outer groove and the inner groove of the specimen carrier are formed and matched with each other, and the groove faces of the two grooves are respectively provided with fine through holes, and the tissue specimen is placed between the outer groove of the sample carrier and the inner groove of the sample carrier, and the The cut surface is attached to the outer groove of the sample carrier.
The histopathological specimen processing system according to claim 4, wherein in the cauterization cavity of the dehydration wax-discharging mechanism, a wax block specimen box carrier strip is installed in the axial direction, and the wax block specimen box is arranged. Installed sequentially on the surface of the wax block specimen box.
A histopathological specimen processing system according to claim 5, wherein said wax block specimen box carrier strip is provided with a front connecting end and a rear connecting end, respectively, and the front frame of said caulking cavity respectively And the rear frame is rotatably connected, and the front connecting end protrudes outwardly from the frame to form a rotating shaft, the rotating shaft is equipped with a gear, the gears of the adjacent wax block specimen box are meshed with each other, and the wax block specimen box in the first position is carried A stepping motor for driving the gear is mounted at the gear.
The histopathological specimen processing system according to claim 1, wherein the wax supplying mechanism comprises: an air compressor, an electric control valve, a wax storage tank and a wax conveying tube, wherein the wax conveying tube is connected to the The block cavity and the wax storage tank of the dehydration waxing mechanism, the liquid paraffin flows between the block cavity and the wax storage tank.
A histopathological specimen processing system according to claim 1, wherein the wax block clamping mechanism comprises a clamping opening and a push rod, and an opening is provided on one side of the clamping opening, the wax block specimen The cartridge is inserted into the clamping opening through the opening, the clamping opening is in an interference fit with the longitudinal direction of the wax block specimen box, and the clamping opening is laterally matched with the wax block specimen box; the other side of the clamping opening is The closed frame is connected with a push rod on the outer side of the closed frame, and the closed frame is connected to the push rod through an elastic body.
The histopathological specimen processing system according to claim 1, wherein the dicing groove mechanism has a groove surface that is inclined downward, and further includes

a blade device, the front surface of the front end of the dicing groove mechanism is laterally disposed with a blade and the blade edge is upward, and the front end of the dicing groove mechanism is disposed at a sipe for loading the blade;

- a left slice chamber, the left slice chamber is provided with a separable sliced piece drawer;

a right slice cavity separated from the left slice cavity by a partition and independent of each other, wherein the right slice cavity is divided into an upper cavity and a lower cavity and separated by a partition, and a slide table is mounted on the left side of the upper cavity. A slide clip is disposed on the right side of the upper chamber, and the slide clip is in the shape of a cavity of the inner interlayer, and a scanning window for dip dyeing and image collection is provided on the front surface thereof, and a slit for the slide into the side is provided on the side surface thereof. The nip of the slide holder corresponds to the lateral position of the surface slide of the slide table; the left side of the slide table is provided with a slide squeegee for pushing the slide piece carrying the tissue slice to the right In the slide clip;

a liquid outflow device comprising a first outflow port and a second outflow port, each controlled by a solenoid valve, the first outflow port being embedded in a partition between the upper chamber and the lower chamber for drawing liquid from the upper chamber into the lower chamber, a second outlet is provided on the bottom side of the lower chamber for discharging the liquid in the lower chamber;

- an intracavity liquid circulation device comprising an inlet pipe, a filter, a water pump and an outlet pipe, the inlet pipe opening being provided in the lower chamber for extracting liquid in the lower chamber by a water pump, the filter being used for Filtering the liquid entering the liquid inlet tube, the nozzle of the liquid outlet tube is embedded in the partition between the upper chamber and the lower chamber, and is used for discharging the liquid extracted in the lower chamber into the upper chamber through the water pump;

a third temperature adjustment mechanism for heating the liquid in the upper chamber;

a slice detecting device comprising a detecting port disposed above the slicing mechanism.
A histopathological specimen processing system according to claim 9, wherein the upper end and the lower end of the slide squeegee are fixed ends, and the upper end and the lower end are symmetrically connected with a sliding piece, and the sliding piece passes through the bottom sliding rail. Lateral reciprocating movement; the bottom of the slide table is further provided with a slide piece for playing a new piece of slide after the top slide is pushed open; a pressure wheel is arranged above the slide table, the pressure wheel The bottom is attached to the slide to prevent the slide from moving perpendicular to the slide when the slide is pushed.
A histopathological specimen processing system according to claim 9, wherein the sipe is an electromagnet sipe.
The histopathological specimen processing system according to claim 1, wherein the first dyeing chamber is regularly provided with a dyeing tank, and the carrier clip is inserted into the dyeing tank, and the second temperature adjusting mechanism is A heating body is included for adjusting the temperature of the liquid in the first dyeing chamber and the baking sheet.
A histopathological specimen processing system according to claim 1, wherein said second dyeing chamber is provided with a slide holder mounting strip, and the slide holder mounting strip is provided with a plurality of slide holder mounting ports in sequence.
A histopathological specimen processing system according to claim 1, wherein said image scanning display unit comprises a scanning mechanism and an image display.
A histopathological specimen processing system according to claim 1, wherein said first pipette, second pipette or third pipette comprises a pipetting gun and a pipetting guide, said The pipetting guide consists of an X-axis pipetting guide and a Y-axis pipetting guide.