CN109443890A - A kind of full-automatic cell staining reaction box - Google Patents
A kind of full-automatic cell staining reaction box Download PDFInfo
- Publication number
- CN109443890A CN109443890A CN201811526748.XA CN201811526748A CN109443890A CN 109443890 A CN109443890 A CN 109443890A CN 201811526748 A CN201811526748 A CN 201811526748A CN 109443890 A CN109443890 A CN 109443890A
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- box body
- reaction box
- reagent
- built
- filling block
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- 238000010186 staining Methods 0.000 title claims abstract description 21
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 51
- 238000010438 heat treatment Methods 0.000 claims abstract description 19
- 238000002347 injection Methods 0.000 claims abstract description 18
- 239000007924 injection Substances 0.000 claims abstract description 18
- 238000004043 dyeing Methods 0.000 abstract description 19
- 239000002699 waste material Substances 0.000 abstract description 7
- 238000012864 cross contamination Methods 0.000 abstract description 5
- 238000004064 recycling Methods 0.000 abstract description 4
- 239000005357 flat glass Substances 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 19
- 239000007788 liquid Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 210000000270 basal cell Anatomy 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000762 glandular Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of full-automatic cell staining reaction boxes, belong to bio-medical technology field, the reaction box is composed of reaction box body and built-in filling block, and reaction box body is rectangular, it is respectively arranged with heating tank in reaction box body surrounding wall outside wall surface, is provided with heating sheet in heating tank；The bottom corner of reaction box body is provided with a reagent injection port；It is oblique to react box body bottom, is provided with level sensing slot in side wall corresponding with reagent injection port adjacent pack bottom；An inlet is provided in the side wall opposite with level sensing slot is provided with, the configuration of the present invention is simple, collection sample introduction waste discharge, cleans, calorifies one, realize the full-automation of cell dyeing, the design of reaction box body bottom angled shape realizes the perfect recycling of reagent or drains, the cross contamination between reagent is avoided while saving reagent, makes coloration result more accurate, and the batch staining reaction suitable for Cell sheet glass is handled.
Description
Technical field
The present invention relates to a kind of full-automatic cell staining reaction boxes, belong to bio-medical technology field, are suitable for various thin
The staining reaction of born of the same parents' slide is handled.
Background technique
Cell dyeing be observation cellular morphology and analysis cell whether most common a kind of skill in the qualitative analyses such as lesion
Art is widely used in Clinicopathologic Diagnosis and experimental study.In order to observe cellular morphology and carry out brush choosing to sick cell, first
It needs to carry out dyeing processing to cell.Conventional cell dyeing be the slide that will be made be put into the reaction box equipped with different reagents into
Row dyeing processing, it is not only cumbersome in the colouring method, multiple reaction cavities are needed, and need special messenger and grasp
Make, is easy to appear operation order mistake.In order to improve such case, up to the present, the cell dyeing mode of improvement, which has, to be passed through
The mobile slide of mechanical arm completes the replacement of reagent to different reaction boxes, or by vacuumizing to reaction box.Such as Chinese patent
Notification number are as follows: CN202720149U, the day for announcing are as follows: 2013.02.06 utility model patent discloses a kind of Full Automatic Liquid basal cell
Dyeing machine passes through the mobile slide of mechanical arm to different reagent tanks.But the device needs multiple cylinder bodies, and holds between reagent
Easy cross contamination.And Chinese patent notification number are as follows: the CN207456903U day for announcing are as follows: a kind of dyeing is disclosed in 2018.06.05
Cylinder, but the staining jar has a single function, and slide is fixed in card slot, and limited amount has a single function, and is not carried out full-automation.
Summary of the invention
In view of the above problems, the purpose of the present invention is to provide one kind to need cell dyeing process only at one
The full-automatic cell staining reaction box completed in reaction box.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of full-automatic cell staining reaction box, the reaction box are composed of reaction box body and built-in filling block,
It is rectangular to react box body, reacts and is respectively arranged with heating tank in the wall outside wall surface of box body surrounding, heating is provided in heating tank
Piece；The bottom corner of reaction box body is provided with a reagent injection port；It is oblique to react box body bottom, from reagent injection port edge
The tilt angle of box body bottom direction be 1.74 °, from reagent injection port along box body bottom direction tilt angle be 1.77 °；With
Level sensing slot is provided in the corresponding side wall in reagent injection port adjacent pack bottom；The side opposite with level sensing slot is provided with
An inlet is provided on wall, inlet is located at below reaction box body upper surface；Reaction inboard wall of cartridge quadrangle connects equipped with arc
Slot, box cover are inserted in by patching leg and are patched in slot；Built-in filling block is rectangle, and handle is equipped at the top of built-in filling block,
The setting of wall surface upper end is fluted at left and right sides of built-in filling block, and the front and back wall surface bottom of built-in filling block slopes inwardly, built-in to fill out
Block is filled to be movably placed in reaction box body.
The built-in filling block is one group.
Due to using above technical scheme, compared with prior art, the present invention having the following advantages that and effect:
1. the present invention can be increased or decreased according to slide quantity and be filled out using the combining form of reaction box body and built-in filling block
The quantity of block is filled, so that slide is fully immersed in reagent.A small number of Coplins are not only suitable for, and can be used for dyeing in batches,
Amount of reagent is also saved while shortening loading time, improves dyeing efficiency.
2. filling block is rectangle built in, facilitates combination, at left and right sides of built-in filling block the setting of wall surface upper end it is fluted and
Front and back wall surface bottom slopes inwardly, and can form channel after combination and reagent is facilitated to circulate.
3. reaction box body bottom uses incline structure, allow the reagent after fully reacting along bottom angled surface current
Enter waste discharge mouth, the perfect recycling of reagent may be implemented and drain, effectively avoids the problem that cross contamination between reagent.
4. being equipped with heating device in reaction box body surrounding wall, good temperature environment is provided for reaction, is greatly improved
The accuracy of cell dyeing.In addition, heating sheet insulation sponge wrapping, realizes quickly heating and heat preservation.
5. being provided with level sensing slot in side wall corresponding with reagent injection port adjacent pack bottom, liquid level is carried out real-time
Monitoring, accurately controls amount of reagent, greatly reduces the waste of reagent.
6. box cover uses plug-in type, unlatching was not only facilitated, but also realize sealing, the volatilization of reagent, enhancing heat preservation can be prevented
Effect.
A kind of full-automatic cell staining reaction box of the invention, has saved reaction box, has avoided the cross contamination between reagent,
The complexity for reducing operation, the slide after making dyeing is cleaner, improves the accuracy rate of experimental result, and reagent can
With recycling, reagent is saved.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention.
Fig. 2 is the front view of Fig. 1.
Fig. 3 is the side view of Fig. 1.
Fig. 4 is the structural schematic diagram of built-in filling block.
Specific embodiment
Below with reference to embodiment and attached drawing to further instruction of the present invention.
Referring to attached drawing.
A kind of full-automatic cell staining reaction box, the reaction box by reaction box body 1 and built-in filling block 4 combination and
At reaction box body 1 is rectangular, reacts and is respectively arranged with heating tank 2 in 1 surrounding side wall outside wall surface of box body, is arranged in heating tank 2
Have heating sheet, heating sheet is connected with temperature switch, and with insulation sponge wrapping；The bottom corner of reaction box body 1 is provided with one
Reagent injection port, reagent injection port are also used as waste discharge mouth；It is oblique to react 1 bottom of box body, from reagent injection port along box body bottom
The tilt angle in 5 direction of portion is 1.74 °, and the tilt angle from reagent injection port along 6 direction of box body bottom is 1.77 °, is realized
The perfect of reagent is recycled and is drained, and effectively avoids the problem that reagent cross contamination；It is corresponding with reagent injection port adjacent pack bottom 6
Side wall on be provided with level sensing slot 3 to liquid level carry out real-time monitoring, realize the accurate control to amount of reagent, greatly reduce
The waste of reagent；An inlet is provided in the side wall opposite with level sensing slot 3 is provided with, inlet is located at reaction box
Below 1 upper surface of body, tap water is from top to bottom sprayed from inlet, not only accelerates the speed that water enters reaction box, Er Qieneng
Reaction box is cleaned cleaner, so that cleaning is more abundant；Reaction 1 inner wall quadrangle of box body is equipped with arc and patches slot, and box cover is logical
It crosses to patch leg and be inserted in and patch in slot, not only facilitated unlatching, but also realize sealing, the volatilization of reagent, enhancing heat preservation effect can be prevented
Fruit；Built-in filling block 4 is rectangle, and length dimension coincide with the length dimension for reacting 1 bottom 5 of box body, using Teflon system
Make, plays the role of etch-proof, fluted, the built-in 4 front and back wall surface of filling block of built-in 4 left and right sides wall surface upper end of filling block setting
Bottom slopes inwardly, and convenient for the circulation of reagent, built-in filling block 4 is movably placed in reaction box body 1.
The built-in filling block 4 is one group, the number of built-in filling block 4 can according to the number of actually required staining rack into
Row increases or decreases, and the staining rack number for being such as put into reaction box body 1 is N, and the number that reaction box body 1 accommodates built-in filling block 4 is
N, need to be put into built-in filling block 4 number be △ n (when △ n=n-N, △ n be 1, be put into built in the number of filling block 4 be 1,
And so on), reduce amount of reagent, reduces dyeing cost.
The application method of full-automatic cell staining reaction box of the present invention is as follows:
(1) Cell sheet glass made is placed on staining rack, the staining rack for being placed with slide is then put into reaction box body 1
In, the number n of built-in filling block 4 is accommodated according to the number N for the dyeing folder for being put into reaction box body 1 and reaction box body 1, can must be put into
The number of built-in filling block 4 is △ n (when △ n=n-N, △ n is 1, the number for being put into built-in filling block 4 is 1, and so on),
It closes the lid.
(2) reagent is reached in reaction box body 1 from reagent bottle by reagent injection port by peristaltic pump, with reagent liquid level
Rise, the position of capacitance sensor monitoring reagent liquid level is installed at reagent liquid level detection slot 3, when the liquid level of reagent reaches setting
When position, stop sample introduction, all pieces in this way can be flooded by reagent.
(3) heating device for reacting the heating tank 2 of 1 surrounding of box body is opened, and when being more than temperature needed for reaction, temperature is opened
Automatic disconnection is closed, heating is stopped.At the same time, reaction can carry out simultaneously.
(4) after completion of the reaction, the reagent of injection can flow into waste discharge mouth from the ramp-like configuration of reaction 1 bottom of box body, into
Row recycling drains.
(5) staining rack is taken out, the slide in staining rack is subjected to microscopy.
(6) tap water enters reaction box body 1 from lateral inflow mouth, is cleaned.After cleaning, water from reaction box body 1
The waste discharge mouth of bottom is discharged.
The dyeing that the present invention is suitable for various Cell sheet glasses is handled, such as is done pap staining processing and Feulgen dyeing and used
Women cervical cell, mammary glandular cell etc..Due to only needing a reaction box, and without other particular/special requirements, greatly reduce anti-
The cost of box is answered, rationally, structure is simple, and low in cost, dyeing quality is excellent and dyeing course is complete for reaction box design of the present invention
Automation.
Claims (2)
1. a kind of full-automatic cell staining reaction box, including reaction box body (1), it is characterised in that: the reaction box is by reacting
Box body (1) and built-in filling block (4) are composed, and reaction box body (1) is rectangular, react box body (1) surrounding side wall outside wall surface
On be respectively arranged with heating tank (2), heating tank is provided with heating sheet in (2)；The bottom corner of reaction box body (1) is provided with one
Reagent injection port；Reaction box body (1) bottom is oblique, and the tilt angle from reagent injection port along box body bottom (5) direction is
1.74 °, the tilt angle from reagent injection port along box body bottom (6) direction is 1.77 °；With reagent injection port adjacent pack bottom
(6) it is provided in corresponding side wall level sensing slot (3)；One is provided in the side wall opposite with level sensing slot (3) is provided with
A inlet, inlet are located at below reaction box body (1) upper surface；Reaction box body (1) inner wall quadrangle is equipped with arc and patches slot, box
Lid is inserted in by patching leg and is patched in slot；Built-in filling block (4) is rectangle, and handle is equipped at the top of built-in filling block (4), interior
It is fluted to set wall surface upper end setting at left and right sides of filling block (4), built-in filling block (4) front and back wall surface bottom slopes inwardly, built-in
Filling block (4) is movably placed in reaction box body (1).
2. a kind of full-automatic cell staining reaction box according to claim 1, it is characterised in that: the built-in filling block
It (4) is one group.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811526748.XA CN109443890A (en) | 2018-12-13 | 2018-12-13 | A kind of full-automatic cell staining reaction box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811526748.XA CN109443890A (en) | 2018-12-13 | 2018-12-13 | A kind of full-automatic cell staining reaction box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109443890A true CN109443890A (en) | 2019-03-08 |
Family
ID=65558142
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811526748.XA Pending CN109443890A (en) | 2018-12-13 | 2018-12-13 | A kind of full-automatic cell staining reaction box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109443890A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111426823A (en) * | 2020-04-30 | 2020-07-17 | 益善生物技术股份有限公司 | Reaction cassette for detection and detection operation method |
| CN114112570A (en) * | 2021-11-12 | 2022-03-01 | 吉林市国科医工科技发展有限公司 | Full-automatic sperm sheet making mechanism based on Diff-Quick staining method |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202793883U (en) * | 2012-08-08 | 2013-03-13 | 刘主国 | Cellular staining reagent cylinder |
| CN203572708U (en) * | 2013-10-29 | 2014-04-30 | 佳木斯大学 | Blood slice and marrow slice dyeing box |
| CN204330457U (en) * | 2015-01-10 | 2015-05-13 | 褚艳凤 | Medical inspection stainer |
| CN205317557U (en) * | 2016-01-14 | 2016-06-15 | 辽宁中医药大学 | Portable cell slide staining rack |
| CN205844063U (en) * | 2016-06-15 | 2016-12-28 | 中国热带农业科学院热带作物品种资源研究所 | A kind of gravity flow glass slide dyeing groove |
| CN206496951U (en) * | 2017-03-03 | 2017-09-15 | 重庆迈德医疗器械有限公司 | A kind of glass frame |
| CN207456902U (en) * | 2017-09-23 | 2018-06-05 | 甘肃农业大学 | A set of cell climbing sheet batch dyeing apparatus |
| CN208140455U (en) * | 2018-03-19 | 2018-11-23 | 遵义医学院附属医院 | A kind of cell, Bacterial stain box |
| CN209606187U (en) * | 2018-12-13 | 2019-11-08 | 武汉纺织大学 | A kind of full-automatic cell staining reaction box |
-
2018
- 2018-12-13 CN CN201811526748.XA patent/CN109443890A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202793883U (en) * | 2012-08-08 | 2013-03-13 | 刘主国 | Cellular staining reagent cylinder |
| CN203572708U (en) * | 2013-10-29 | 2014-04-30 | 佳木斯大学 | Blood slice and marrow slice dyeing box |
| CN204330457U (en) * | 2015-01-10 | 2015-05-13 | 褚艳凤 | Medical inspection stainer |
| CN205317557U (en) * | 2016-01-14 | 2016-06-15 | 辽宁中医药大学 | Portable cell slide staining rack |
| CN205844063U (en) * | 2016-06-15 | 2016-12-28 | 中国热带农业科学院热带作物品种资源研究所 | A kind of gravity flow glass slide dyeing groove |
| CN206496951U (en) * | 2017-03-03 | 2017-09-15 | 重庆迈德医疗器械有限公司 | A kind of glass frame |
| CN207456902U (en) * | 2017-09-23 | 2018-06-05 | 甘肃农业大学 | A set of cell climbing sheet batch dyeing apparatus |
| CN208140455U (en) * | 2018-03-19 | 2018-11-23 | 遵义医学院附属医院 | A kind of cell, Bacterial stain box |
| CN209606187U (en) * | 2018-12-13 | 2019-11-08 | 武汉纺织大学 | A kind of full-automatic cell staining reaction box |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111426823A (en) * | 2020-04-30 | 2020-07-17 | 益善生物技术股份有限公司 | Reaction cassette for detection and detection operation method |
| CN114112570A (en) * | 2021-11-12 | 2022-03-01 | 吉林市国科医工科技发展有限公司 | Full-automatic sperm sheet making mechanism based on Diff-Quick staining method |
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