CN114112570A - Full-automatic sperm sheet making mechanism based on Diff-Quick staining method - Google Patents
Full-automatic sperm sheet making mechanism based on Diff-Quick staining method Download PDFInfo
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- CN114112570A CN114112570A CN202111340805.7A CN202111340805A CN114112570A CN 114112570 A CN114112570 A CN 114112570A CN 202111340805 A CN202111340805 A CN 202111340805A CN 114112570 A CN114112570 A CN 114112570A
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- 230000007246 mechanism Effects 0.000 title claims abstract description 21
- 238000007447 staining method Methods 0.000 title claims description 6
- 239000007788 liquid Substances 0.000 claims abstract description 61
- 239000011521 glass Substances 0.000 claims abstract description 41
- 238000000034 method Methods 0.000 claims abstract description 26
- 238000001035 drying Methods 0.000 claims abstract description 23
- 238000007664 blowing Methods 0.000 claims abstract description 21
- 238000003860 storage Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000004043 dyeing Methods 0.000 claims abstract description 14
- 238000001514 detection method Methods 0.000 claims description 11
- 239000003814 drug Substances 0.000 claims description 4
- 230000009303 sperm storage Effects 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 3
- 238000010186 staining Methods 0.000 description 10
- 230000008569 process Effects 0.000 description 6
- 210000000582 semen Anatomy 0.000 description 5
- 230000036541 health Effects 0.000 description 4
- 230000008520 organization Effects 0.000 description 4
- 230000007547 defect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000009971 piece dyeing Methods 0.000 description 2
- 238000004544 sputter deposition Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000004048 vat dyeing Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
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- Engineering & Computer Science (AREA)
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Abstract
A full-automatic sperm sheet making mechanism based on a Diff-Quick dyeing method comprises a dye vat module, a drying module, a gripper module, a display and operation module, a sheet storage module and a shell frame, wherein the display and operation module is arranged on the side surface of the shell frame, the dye vat module comprises four fixed liquid dye vats which are connected side by side and fixed at the bottom of the shell frame, a first dye liquid dye vat, a second dye liquid dye vat and a flowing water vat, the drying module comprises an air blower and two air blowing devices, the air blower is fixed at one side of the shell frame, the outlet of the air blower is connected with the two air blowing devices, the gripper module comprises an X-direction sliding table, a Y-direction sliding table, a Z-direction sliding table and a rotary gripper sheet storage module, the sheet storage module comprises a slide glass placing frame and a slide glass storing frame, and the sheet storage module is positioned at one side of the dye vat module and the drying module and is positioned below the rotary gripper Dyeing in a dye vat, drying and storing.
Description
Technical Field
The invention belongs to the field of seminal plasma or sperm microscopic detection in medical detection, and particularly relates to a full-automatic sperm sheet making mechanism based on a Diff-Quick staining method.
Background
Sperm quality directly affects male fertility, wherein sperm morphology detection is an important index for measuring sperm quality. At present, the relevant departments of hospitals usually adopt the method specified in the fifth edition of the manual of the world health organization human semen examination and treatment laboratory, and the sperm staining smear is made and then observed by a microscope, so as to judge the sperm morphology index. The automatic staining smear equipment on the market is not suitable for sperm staining smear due to the special characteristics of the sample. The hospital mainly adopts a manual operation mode to make a sperm smear based on a Diff-Quick staining method.
At present, the traditional manual mode is mainly adopted for sperm staining in hospitals and scientific research laboratories, and the technical defects are as follows: 1. the manual operation of personnel is easy to generate sputtering pollution; 2. the speed of single piece dyeing is slow, and the efficiency is low; 3. human errors exist, and the accuracy is low; 4. the labor is consumed, and the sample is inspected as soon as possible.
The existing slice dyeing machine on the market mainly comprises a membrane method, a centrifugal method, a cell capture method, a spring method and the like, and has the technical defects that: the dyeing process is not completely automatic; the speed of single piece dyeing is slow; the cell background is not clean; cannot be integrated into other devices.
In view of the above, the invention provides a sperm slide mechanism, which automatically completes the preparation of sperm stained smears according to the quick staining procedure of sperm morphology given in the fifth edition of the manual of the world health organization human sperm examination and treatment laboratory.
Disclosure of Invention
The invention provides a full-automatic sperm smear making mechanism based on a Diff-Quick dyeing method, which adopts the structural design of an intelligent reduction laboratory method to complete the automatic grabbing, dye vat dyeing, drying and smear storage processes of a sperm smear at high speed and accurately.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
a full-automatic sperm flaking mechanism based on a Diff-Quick dyeing method is composed of a dye vat module, a drying module, a gripper module, a display and operation module, a flake storage module and a shell frame, wherein the dye vat module, the drying module, the gripper module and the flake storage module are all located in the shell frame, the display and operation module is installed on the side face of the shell frame, the dye vat module comprises four fixed liquid dye vats which are connected side by side and fixed at the bottom of the shell frame, a first dye liquid dye vat, a second dye liquid dye vat and a flowing water vat, the bottoms of the fixed liquid dye vats, the first dye liquid dye vat and the second dye vat are respectively connected with a conveying pump through pipelines, the other end of each conveying pump is connected with a medicine bottle, the upper portion and the lower portion of the flowing water vat are respectively connected with a first liquid path pump and a second liquid path pump through pipelines, the drying module comprises an air blower, Two blast apparatus, the air-blower is fixed in shell frame one side and its export links to each other with two blast apparatus, two blast apparatus fixed connection are in shell frame and the symmetrical position in the front and back both sides of dye vat module, the tongs module includes X to slip table, Y to slip table, Z to slip table, rotatory tongs, two Z sets up the both sides in shell frame to the slip table, Y slides to the slip table and sets up on Z to the slip table, X slides to the slip table and sets up on Y is to the slip table, rotatory tongs slides and sets up on X is to the slip table, deposit the piece module and deposit the piece frame including slide glass put the piece frame, slide glass including, deposit the piece module and be located one side of dye vat module and stoving module, and be located the below of rotatory tongs.
Preferably, the fixed liquid dye vat, the first dye vat and the second dye vat have the same structure, and take the fixed liquid dye vat as an example, the fixed liquid dye vat is composed of a dye vat and a dye vat cover, the dye vat cover is buckled and fixed on the top of the dye vat, a glass slide inlet is arranged on the dye vat cover, a recess is arranged on one side of the glass slide inlet, a sponge is embedded in the recess, a liquid inlet is arranged at the lower part of the dye vat, and two photoelectric liquid level switches are arranged at the upper part of the dye vat.
Preferably, the blowing device is a cuboid, an air inlet is formed in the side face of the blowing device, four long-strip-shaped air outlets are formed in the front face of the blowing device, the air outlets are communicated with the air inlet, the air inlet is connected with an outlet of the air blower, and the air blower is connected with the blowing device.
Preferably, the display and operation module is composed of a display screen and a touch screen, the display screen can display real-time information of the whole mechanism, and the touch screen can control actions of all the mechanisms.
Preferably, the front end of the shell frame is fixedly connected with a horizontal detection platform.
Preferably, two capacitive proximity switches are further arranged on the rotary gripper.
Preferably, the structure of the running water tank is the same as that of the fixed liquid dye tank, and a photoelectric liquid level switch is arranged at the upper part of the running water tank.
The invention has the beneficial effects that:
the invention can replace the manual staining procedure of the rapid staining procedure of the sperm morphology given in the fifth edition of the manual for human semen examination and treatment laboratory manual of world health organization, can realize the rapid staining procedure method of the sperm morphology not limited to the fifth edition of the manual for human semen examination and treatment laboratory manual of world health organization, and realizes the whole processes of automatic grabbing, staining in a staining jar, drying and storing of a full-automatic sperm smear through a touch display screen. The whole process is fully automatic, the dyeing speed is high, sputtering pollution is avoided, manpower is saved, and the quality is improved.
Drawings
FIG. 1 is a front view of the present invention;
FIG. 2 is a top view of the present invention;
FIG. 3 is a schematic view of the internal structure of the present invention;
FIG. 4 is a schematic structural view of a fixed liquor dye vat of the present invention;
FIG. 5 is a schematic structural view of an air blowing device according to the present invention;
FIG. 6 is a flow chart;
FIG. 7 is a schematic view of an embodiment;
the reference symbols shown in the figures are: 1-display and operation module, 2-shell frame, 3-fixed liquid dye vat, 4-first dye vat, 5-second dye vat, 6-flowing water vat, 7-liquid medicine bottle, 8-delivery pump, 9-first liquid path pump, 10-second liquid path pump, 11-blower, 12-blower, 13-X direction sliding table, 14-Y direction sliding table, 15-Z direction sliding table, 16-rotary gripper, 17-slide glass rack, 18-slide glass rack, 19-detection platform, 20-capacitance approach switch, 21-slide glass, 301-dye vat, 302-dye vat cover, 303-slide glass inlet, 304-recess, 305-sponge, 306-liquid inlet, 307-photoelectric liquid level switch, 1201-air inlet, 1202-air outlet.
Detailed Description
The following describes the present design in detail with reference to the accompanying drawings.
As shown in figures 1-5, a full-automatic sperm flaking mechanism based on Diff-Quick dyeing method comprises a dye vat module, a drying module, a gripper module, a display and operation module 1, a sperm storage module and a shell frame 2, wherein the dye vat module, the drying module, the gripper module and the sperm storage module are all positioned in the shell frame 2, the display and operation module 1 is arranged on the side surface of the shell frame 2, the dye vat module comprises four fixed liquid dye vats 3 which are connected side by side and fixed at the bottom of the shell frame, a first dye liquid dye vat 4, a second dye liquid dye vat 5 and a running water vat 6, the bottoms of the fixed liquid dye vat 3, the first dye liquid dye vat 4 and the second dye liquid dye vat 5 are respectively connected with a delivery pump 8 through pipelines, the other end of each delivery pump 5 is connected with a liquid medicine bottle 7, the upper part and the lower part of the running water vat 6 are respectively connected with a first liquid path pump 9, a second liquid path pump 7 through pipelines, A second liquid path pump 10, a fixed liquid dye vat 3, a first dye vat 4 and a second dye vat 5 have the same structure, taking the fixed liquid dye vat 3 as an example, the fixed liquid dye vat is composed of a dye vat 301 and a dye vat cover 302, the dye vat cover 302 is buckled and fixed on the top of the dye vat 301, a glass slide inlet 303 is arranged on the dye vat cover 302, one side of the glass slide inlet 303 is provided with a dent 304, a sponge 305 is embedded in the dent 304, the lower part of the dye vat 301 is provided with a liquid inlet 306, the upper part of the dye vat 301 is provided with two photoelectric liquid level switches 307, the two photoelectric liquid level switches 307 form a high liquid level line and a low liquid level line, the liquid level is monitored in real time, the dye solution is supplied in time after the dye slice is ensured, the sponge 305 is a high water absorption sponge slice, the redundant solution is filtered and recovered while the redundant solution of the glass slide 21 is absorbed, the solution consumption can be reduced to the maximum, the drying time is reduced, the slice making efficiency is improved, the structure of the flowing water vat 6 is the same as that of the fixed liquid dye vat 3, and a photoelectric liquid level switch is arranged at the upper part of the water tank. The drying module comprises a blower 11 and two blowing devices 12, the blower 11 is fixed on one side of the shell frame 2, the outlet of the blower is connected with the two blowing devices 12, the two blowing devices 12 are respectively and fixedly connected in the shell frame 2 and symmetrically positioned on the front side and the rear side of the dye vat module, the blowing devices 12 are cuboid, the side surfaces of the blowing devices are provided with air inlets 1201, the front sides of the blowing devices 12 are provided with four strip-shaped air outlets 1202, the air outlets 1202 are communicated with the air inlets 1201, the air inlets 1201 are connected with the outlet of the blower 11, the blower 11 is further connected with the blowing devices 12, the blower 11 is provided with a double-air-volume multi-channel integrated air outlet, the air volume and the temperature can be adjusted and controlled, the drying module is used for drying the glass slide 21 soaked with the dye liquor, the gripper module comprises an X-direction sliding table 13, a Y-direction sliding table 14, a Z-direction sliding table 15 and a rotary gripper 16, and the two Z-direction sliding tables 15 are arranged on the two sides in the shell frame 2, y slides to slip table 14 and sets up on Z is to slip table 15, X slides to slip table 13 and sets up on Y is to slip table 14, rotatory tongs 16 slides and sets up in X is to slip table 13, Y is to slip table 14, Z can make rotatory tongs 16 free motion in three-dimensional space to the combined motion of slip table 15, realize snatching of each position, still be equipped with two capacitanc proximity switch 20 on the rotatory tongs 16, rotatory tongs 16 is purchase the piece outward, be used for dying grabbing of piece in-process, transfer, rotatory slide 21, and capacitanc proximity switch 20 is used for detecting the position of placing of slide 21, be convenient for accurately snatch. The slide storage module comprises a slide placing frame 18 and a slide storage frame 18, the slide placing frame 17 is used for temporarily storing the slide 21 subjected to scraping, the slide storage frame 18 is used for timely storing the detected slide 21, and the slide storage module is located on one side of the dye vat module and the drying module and below the rotary gripper 16. The front end of the housing frame 2 is also fixedly connected with a horizontal detection platform 19, and the detection platform 19 provides a dyed slide 21 for the detection device.
The display and operation module 1 is composed of a display screen and a touch screen, the display screen can display real-time information of the whole mechanism, and the touch screen can only be connected with corresponding motion assemblies in an automatic control mode and can control actions of all the mechanisms.
Examples
As shown in fig. 6-7, the main working process of the present invention is that the gripping module grips the slide glass 21 coated with the semen and immerses the slide glass into the stationary liquid dye vat 3 in the dye vat module for 15 seconds, the gripping module takes out the slide glass 21 and moves the slide glass onto the sponge 305 absorbed on the stationary liquid dye vat 3 to suck away the excess solution, and meanwhile, the drying module dries the slide glass 21 for 15 seconds; then the dried glass slide 21 is grabbed by the grabbing module and immersed in the first dye solution dye vat 4 in the dye vat module for 10 seconds, the glass slide 21 is taken out by the grabbing module and moved to the sponge absorbing water on the first dye solution dye vat 4, the redundant solution is sucked away, and meanwhile, the drying module dries the glass slide 21 for 15 seconds; then the gripper module grips the dried glass slide 21 and immerses the dried glass slide into a second dye solution dye vat 5 in the dye vat module for 5 seconds, the gripper module takes out the glass slide 21 and moves the glass slide to a sponge absorbing water on the second dye solution dye vat 5, and redundant solution is sucked away; and then the gripper module grips the glass slide 21 dyed with the dye solution and immerses the glass slide into the flow cylinder 6 in the dye cylinder module for 15 seconds, the gripper module takes out the glass slide 21 and moves the glass slide to a water-absorbing sponge sheet on the flow cylinder 6 to suck away the redundant solution, meanwhile, the drying module dries the glass slide for 15 seconds, and the sperm dyeing is finished, the gripper module grips the glass slide 21 dyed with the sheet, the gripper 16 rotates for 90 degrees to enable the glass slide 21 to be placed on the detection platform 19 in a horizontal state, the gripper 16 rotates for 90 degrees to enable the glass slide 21 to be restored to a vertical state after the detection is finished, and the capacitive proximity switch 20 on the gripper 16 detects the position of the glass slide in the glass slide storage module to realize intelligent glass storage. The whole process can be monitored and processed in real time through the display and operation module 1.
Claims (7)
1. A full-automatic sperm flaking mechanism based on a Diff-Quick dyeing method is characterized by comprising a dye vat module, a drying module, a gripper module, a display and operation module, a sperm storage module and a shell frame, wherein the dye vat module, the drying module, the gripper module and the sperm storage module are all positioned in the shell frame, the display and operation module is installed on the side surface of the shell frame, the dye vat module comprises four fixed liquid dye vats which are connected side by side and fixed at the bottom of the shell frame, a first dye liquid dye vat, a second dye liquid dye vat and a flow vat, the bottoms of the fixed liquid dye vats, the first dye liquid dye vat and the second dye liquid dye vat are respectively connected with a conveying pump through pipelines, the other end of each conveying pump is connected with a liquid medicine bottle, the upper part and the lower part of the flow vat are respectively connected with a first liquid path pump and a second liquid path pump through pipelines, the drying module comprises an air blower and two air blowing devices, the air blower is fixed on one side of a shell frame, an outlet of the air blower is connected with the two air blowing devices, the two air blowing devices are fixedly connected in the shell frame and are symmetrically arranged on the front side and the rear side of the dye vat module, the gripper module comprises an X-direction sliding table, a Y-direction sliding table, a Z-direction sliding table and a rotary gripper, the Z-direction sliding table is arranged on the two sides of the shell frame, the Y-direction sliding table is arranged on the Z-direction sliding table, the X-direction sliding table is arranged on the Y-direction sliding table, the rotary gripper is arranged on the X-direction sliding table in a sliding mode, the slide storage module comprises a slide glass placing rack and a slide glass storage rack, and the slide storage module is located on one side of the dye vat module and the drying module and is located below the rotary gripper.
2. A full-automatic sperm flaking mechanism based on a Diff-Quick dyeing method is characterized in that a fixed liquid dye vat, a first dye vat and a second dye vat are identical in structure, the fixed liquid dye vat is taken as an example and comprises a dye vat and a dye vat cover, the dye vat cover is buckled and fixed on the top of the dye vat, a glass slide inlet is formed in the dye vat cover, a recess is formed in one side of the glass slide inlet, a piece of sponge is embedded in the recess, a liquid inlet is formed in the lower portion of the dye vat, and two photoelectric liquid level switches are mounted on the upper portion of the dye vat.
3. The full-automatic sperm sheet making mechanism based on the Diff-Quick dyeing method is characterized in that the blowing device is a cuboid, an air inlet is formed in the side face of the blowing device, four long-strip-shaped air outlets are formed in the front face of the blowing device, the air outlets are communicated with the air inlet, the air inlet is connected with an outlet of an air blower, and the air blower is connected with the blowing device.
4. The full-automatic sperm production mechanism based on the Diff-Quick dyeing method is characterized in that the display and operation module consists of a display screen and a touch screen, the display screen can display real-time information of the whole mechanism, and the touch screen can control actions of all the mechanisms.
5. A full-automatic sperm production mechanism based on a Diff-Quick staining method is characterized in that the front end of a shell frame is fixedly connected with a horizontal detection platform.
6. A full-automatic sperm production mechanism based on a Diff-Quick staining method is characterized in that two capacitive proximity switches are further arranged on a rotary gripper.
7. A full-automatic sperm preparation mechanism based on a Diff-Quick dyeing method is characterized in that the structure of a running water tank is the same as that of a fixed liquid dye tank, and a photoelectric liquid level switch is arranged at the upper part of the running water tank.
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CN202111340805.7A CN114112570A (en) | 2021-11-12 | 2021-11-12 | Full-automatic sperm sheet making mechanism based on Diff-Quick staining method |
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CN109443890A (en) * | 2018-12-13 | 2019-03-08 | 武汉纺织大学 | A kind of full-automatic cell staining reaction box |
CN110208068A (en) * | 2019-06-15 | 2019-09-06 | 泉州市科茂利通智能科技有限公司 | A kind of filling of dyeing liquor and waste liquid draw two-in-one mechanical arm and all-in-one machine is dyed in film-making |
CN110857181A (en) * | 2018-08-23 | 2020-03-03 | 达科为(深圳)医疗设备有限公司 | Novel intelligence film sealing machine |
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EP3792828A1 (en) * | 2019-09-16 | 2021-03-17 | Airamatrix Private Limited | Methods and systems for automated assessment of spermatogenesis |
CN112611620A (en) * | 2020-12-15 | 2021-04-06 | 成都朴华科技有限公司 | Automatic change sperm staining equipment |
CN212988983U (en) * | 2020-07-21 | 2021-04-16 | 上海威进士生物科技有限公司 | Dip dyeing type automatic dyeing device |
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2021
- 2021-11-12 CN CN202111340805.7A patent/CN114112570A/en active Pending
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KR20020041172A (en) * | 2000-11-27 | 2002-06-01 | 이면우 | Automatic Cytology Preparing System |
CN202351113U (en) * | 2011-12-02 | 2012-07-25 | 苏州海路生物技术有限公司 | Slide staining machine |
CN206710176U (en) * | 2017-05-11 | 2017-12-05 | 麦克奥迪(厦门)医疗诊断系统有限公司 | Mini multifunctional dyeing work station |
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CN109443890A (en) * | 2018-12-13 | 2019-03-08 | 武汉纺织大学 | A kind of full-automatic cell staining reaction box |
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CN211179211U (en) * | 2019-11-11 | 2020-08-04 | 镇江蓝勃医疗器械有限公司 | Dyeing tank with liquid level detection function |
CN212988983U (en) * | 2020-07-21 | 2021-04-16 | 上海威进士生物科技有限公司 | Dip dyeing type automatic dyeing device |
CN112611620A (en) * | 2020-12-15 | 2021-04-06 | 成都朴华科技有限公司 | Automatic change sperm staining equipment |
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