CN206710176U - Mini multifunctional dyeing work station - Google Patents
Mini multifunctional dyeing work station Download PDFInfo
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- CN206710176U CN206710176U CN201720521666.0U CN201720521666U CN206710176U CN 206710176 U CN206710176 U CN 206710176U CN 201720521666 U CN201720521666 U CN 201720521666U CN 206710176 U CN206710176 U CN 206710176U
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- workspace
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- dyeing
- liquid
- water bath
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Abstract
Mini multifunctional dyeing work station, including casing, the water bath being embedded in casing, temperature-controlling system, with alloy melt stirring device, automaton and discharging tube, described water bath is divided into liquid workspace and dyeing workspace, described temperature-controlling system controls the temperature with liquid workspace and the temperature of dyeing workspace simultaneously, described is fixed in casing and in the lower section with liquid workspace with alloy melt stirring device, water level in automatic control system control water bath, temperature-controlling system, operating time with alloy melt stirring device and dyeing workspace, described discharging tube connects with water bath, water in water bath is discharged by discharging tube.Using the technical solution of the utility model, there is high compact, temperature-controlled precision, Jing Yin, long lifespan, energy-conservation, can be achieved the rapid dyeing under extreme condition, easy to operate, safety is dying-stable, reliable, meets the demand of Different hospital.
Description
Technical field
Pathological examination instrument is the utility model is related to, a kind of slide for pathology censorship is referred in particular to and carries out nucleus
The mini multifunctional dyeing work station of DNA dyeing.
Background technology
The research of cytomorphology now, it has been not limited only to observe under conventional microscope, has more employed new technology and newly
Method, especially pathological section scan-image analysis system.The system is typically by slide loader, biomicroscope, system control
Center processed(Host computer, keyboard, mouse etc.), CCD video frequency pick-up heads/camera, barcode reader, electronic loading scanning platform and
Application software etc. forms.For clinically as aid to cell of interest according to the specific color of cell, concentration/strong
Degree, size/dimension, pattern/pattern and shape are detected, classified and counted, and help pathologist to divide pathological section
Analysis, improve diagnostic accuracy.With image analysis technology, first have to dye cellular morphology, wherein with thionine dyes, pair
The nucleus chemical dyeing method that rosaniline dyes, azure dyes are established is relatively stable, reliable.Due to the preparation and dyeing of three kinds of dyestuffs
Condition is more harsh, and particularly with liquid, dyeing temperature is inconsistent or temperature control is inaccurate, easily causes dyeing to fail, influences to diagnose
As a result with the timely granting of report.
Utility model content
The technical problems to be solved in the utility model is to provide a kind of mini multifunctional dyeing work station, possesses while full
The characteristics of foot is with liquid, dyeing, accurate temperature control.
In order to solve the above-mentioned technical problem, the technical solution of the utility model is:Mini multifunctional dyeing work station, its
Be characterised by, including casing, the water bath being embedded in casing, temperature-controlling system, with alloy melt stirring device, automaton and row
Liquid pipe, described water bath are divided into liquid workspace and dyeing workspace, and described temperature-controlling system controls simultaneously matches somebody with somebody liquid workspace
Temperature and dyeing workspace temperature, it is described that with alloy melt stirring device to be fixed on casing interior and under with liquid workspace
Side, automatic control system control water bath in water level, temperature-controlling system, with alloy melt stirring device and dye workspace operation when
Between, described discharging tube is connected with water bath, and the water in water bath is discharged by discharging tube.
Further, described to place plate with being provided with blender jar in liquid workspace, blender jar places plate provided with least one
Individual blender jar putting hole, elastic force geometrical clamp is provided with around blender jar putting hole, blender jar, bullet are placed with blender jar putting hole
Power geometrical clamp fixes blender jar and then controls the position height of blender jar, described to be disposed therein one with alloy melt stirring device and stir
The lower section of bottle putting hole is mixed, and the center of circle of the central point with alloy melt stirring device and blender jar putting hole is on coaxial line, it is described
With a plexiglass tent is additionally provided with above liquid workspace, the outer surface of described plexiglass tent is coated with preventing water drops holiday
Layer.
Further, described alloy melt stirring device of matching somebody with somebody includes metal cap, two pole pieces, pole piece fixed plate and rotates electricity
Machine, rotary electric machine stretch out a rotary shaft, and pole piece fixed plate is fixed on the rotating shaft, and two pole piece homopolarities are relative and symmetrical
It is distributed and is fixed in pole piece fixed plate, metal cap covers pole piece fixed plate, pole piece and rotary electric machine, and described two
On coaxial line, described is placed in alloy melt stirring device in the center of circle of the symmetrical central point of individual pole piece and blender jar putting hole
There is metal stirring sheet in the blender jar of top, described rotates with alloy melt stirring device driving metal stirring sheet and then stir blender jar
Interior liquid.
Further, described temperature-controlling system includes heating electrode, temperature detector and temperature controller, described heating electrode
It is distributed in water bath, to being arranged on liquid workspace and the heating of dyeing workspace, temperature detector in water bath, temperature controller control
System heating electrode work, temperature detector temperature sensor simultaneously feeds back to temperature controller, temperature controller and then is adjusted.
Further, described dyeing workspace is provided with 4 staining jar positions, and staining jar, staining jar are placed in staining jar position
Staining rack is inside placed with, described staining jar and staining rack use PP materials.
Further, described automaton includes Liquid level inductor, electronic type time switch, embedded fixed
When device and control circuit, described embedded timer be embedded on casing, Liquid level inductor is placed in water bath, electronics
Formula time switch controls the switch of control circuit, and control circuit controls temperature-controlling system, with the water level in alloy melt stirring device, water bath
With the operating time of dyeing workspace.
The colouring method of mini multifunctional dyeing work station, it is characterised in that comprise the following steps:
Step 1:Added water into water bath and do not cross Liquid level inductor peak, opening for electronic type time switch is set
Close the time, start preheating, dyeing temperature is set by temperature controller;
Step 2:Dyeing liquor is configured with liquid workspace, fixer, acidolysis are added respectively in the staining jar of dyeing workspace
Liquid, the dyeing liquor with the configuration of liquid workspace, rinsing liquid;
Step 3:Pathological section is inserted in staining rack, staining rack is put into fixer and fixed, timer starts to count
When;
Step 4:After the countdown concluded, staining rack is removed, clear water rinses;
Step 5:Staining rack after the completion of flushing is put into acidolysis in acid hydrolysis solution, and timer starts countdown;
Step 6:Repeat step 4, the staining rack after the completion of flushing, which is put into dyeing liquor, to be dyed, and timer starts countdown;
Step 7:Repeat step 4, the staining rack after the completion of flushing, which is put into rinsing liquid, to be rinsed, and timer starts countdown;
Step 8:After the countdown concluded, staining rack, section dehydration, mounting are taken out.
Further, the collocation method of the dyeing liquor in step 2, by taking 500ml blender jars as an example, comprise the following steps:
Step a:392ml distilled water is measured in blender jar, reagent A and each 0.4g of reagent B is added, is eventually adding
8ml 5mol/L hydrochloric acid is in blender jar;
Step b:Blender jar is placed in the blender jar putting hole in liquid workspace, and adjusts blender jar by elastic force geometrical clamp
Placing height and fixation, open and are stirred with alloy melt stirring device;
Step c:Funnel and filter paper are placed in another blender jar with liquid workspace;
Step d:The dyeing liquor that step b stirrings are completed is filtered by step c blender jar, completes configuration.
Further, the reagent A described in step a is one kind in thionine, reddish black or paramagenta;Described reagent B is
Sodium pyrosulfite or potassium metabisulfite.
Further, dyeing temperature described in step 1 is 25 DEG C, 35 DEG C or 55 DEG C;Step 3, timing described in 5,6,7
It is 3min, 5min, 10min, 25min, 30min, 40min, 50min, 60min or 75min that device, which sets the time,;In step 3
Fixer is two or more composition in formaldehyde, ethanol, methanol, glacial acetic acid;Acid hydrolysis solution described in step 5 is that hydrochloric acid is molten
Liquid, concentration 1mol/L, 3 mol/L, 5 mol/L or 6 mol/L;Rinsing liquid described in step 7 is distilled water or burnt sulfurous
Acid sodium aqueous solution.
The beneficial effects of the utility model and advantage are:
1st, water bath is combined by the work station with magnetic force with alloy melt stirring device, can carry out magnetic agitation to dyeing liquor
While solution temperature is reached reaction desirable value, and heat evenly, temperature fluctuation is small;
2nd, a set of temperature-controlling system is shared with liquid and dyeing workspace, avoids producing difference, shadow with liquid temperature degree and dyeing temperature
Ring Color;
3rd, elastic force geometrical clamp is introduced, the bottom for avoiding container is in vacant state, causes poor heating, solution to incline
, or even the failure of an experiment;
4th, staining jar/frame makes the big rule of fast staining under pyroreaction system using high temperature resistant, corrosion resistant PP materials
Mould application becomes a reality, and hydrophobic staining rack attaching liq is few, avoids dye liquor from wasting;
5th, plexiglass tent surface is covered with special preventing water drops holiday layer, and when effectively avoiding filtering dye liquor, water droplet drops onto Lou
On bucket;
6th, electronic type time switch, solve the worry that operator forgets switch often, improve Electrical Safety;
7th, complete machine has small volume, little power consumption, Jing Yin, long lifespan, practical, corrosion resistant advantage.
Brief description of the drawings
Specific embodiment of the present utility model is described in further detail below in conjunction with the accompanying drawings.
Fig. 1 is the utility model structural perspective;
Fig. 2 is the utility model structure diagram;
Fig. 3 is the utility model water bath internal structure schematic diagram
Fig. 4 is staining jar and staining rack the first structural representation in the utility model;
Fig. 5 is second of structural representation of staining jar and staining rack in the utility model
Fig. 6 is elastic force geometrical clamp configuration diagram in the utility model;
Fig. 7 is that the utility model matches somebody with somebody alloy melt stirring device structural representation;
Fig. 8 is the utility model automaton control principle schematic diagram;
Fig. 9 is the utility model Color schematic diagram one;
Figure 10 is the utility model Color schematic diagram two.
Embodiment
With reference to the accompanying drawings and detailed description, the utility model is described further.
As Figure 1-5, mini multifunctional dyeing work station, including casing 10, the water bath that is embedded in casing 10
20th, temperature-controlling system, divide for liquid work with alloy melt stirring device 50, automaton 30 and discharging tube 40, described water bath 20
Make area 21 and dyeing workspace 22, described dyeing workspace 22 is provided with 4 staining jar positions, staining jar is placed in staining jar position
221, staining rack 222 is placed with staining jar 221, described staining jar 221 and staining rack 222 use PP materials, and structure has two
Kind form, specifically as shown in Figure 4,5, described temperature-controlling system control the temperature for matching somebody with somebody liquid workspace 21 and dye workspace 22 simultaneously
Temperature, it is described to be fixed on alloy melt stirring device 50 in casing 10 and in the lower section with liquid workspace 21, automatically control and be
It is water level, temperature-controlling system in the control water bath 20 of system 30, the operating time with alloy melt stirring device 50 and dyeing workspace 22, described
Discharging tube 40 connected with water bath 20, the water in water bath 20 is discharged by discharging tube 40.
Shown in Fig. 2,3,6, plate 211 is placed with blender jar is provided with liquid workspace 21, blender jar is placed plate 211 and is provided with extremely
Lack a blender jar putting hole 212, elastic force geometrical clamp 213, blender jar putting hole 212 are provided with around blender jar putting hole 212
Blender jar 214 is inside placed with, elastic force geometrical clamp 213 fixes blender jar 214 and then controls the position height of blender jar 214, described
Be disposed therein the lower section of a blender jar putting hole 212, and the center with alloy melt stirring device 50 with alloy melt stirring device 50
The center of circle with blender jar putting hole 212 is put on coaxial line, the described top with liquid workspace 21 is additionally provided with a lucite
Cover 215, the outer surface of described plexiglass tent 215 is coated with preventing water drops holiday layer.
Fig. 2, include 51, two pole pieces 52 of metal cap, pole piece fixed plate 53 shown in 7, with alloy melt stirring device 50 and turn
Dynamic motor 54, rotary electric machine 54 stretch out a rotary shaft, and pole piece fixed plate 53 is fixed on the rotating shaft, two homopolarities of pole piece 52
Relative and symmetrical and be fixed in pole piece fixed plate 53, metal cap 51 covers pole piece fixed plate 53, pole piece
52 and rotary electric machine 54, the center of circle of described two symmetrical central points of pole piece 52 and blender jar putting hole 212 is same
On axis, described being placed in the blender jar 214 with the top of alloy melt stirring device 50 has metal stirring sheet 215, described to be stirred with liquid
Mixing device 50 drives metal stirring sheet 215 to rotate and then stir the liquid in blender jar 214.
Fig. 3, shown in 8, temperature-controlling system includes heating electrode 61, temperature detector and temperature controller, described heating electrode 61
It is distributed in water bath 20, to being heated with liquid workspace 21 and dyeing workspace 22 simultaneously, temperature detector is arranged in water bath 20,
Temperature controller control heating electrode 61 work, temperature detector temperature sensor simultaneously feeds back to temperature controller, temperature controller and then
It is adjusted.
Shown in Fig. 8, described automaton includes Liquid level inductor, electronic type time switch, embedded fixed
When device and control circuit, described embedded timer be embedded on casing, Liquid level inductor is placed in water bath, electronics
Formula time switch controls the switch of control circuit, and control circuit controls temperature-controlling system, with the water level in alloy melt stirring device, water bath
With the operating time of dyeing workspace.
The colouring method of above-mentioned mini multifunctional dyeing work station, comprises the following steps:
Step 1:Added water into water bath and do not cross Liquid level inductor peak, opening for electronic type time switch is set
Close the time, start preheating, dyeing temperature is set by temperature controller;
Step 2:Dyeing liquor is configured with liquid workspace, fixer, acidolysis are added respectively in the staining jar of dyeing workspace
Liquid, the dyeing liquor with the configuration of liquid workspace, rinsing liquid;
Step 3:Pathological section is inserted in staining rack, staining rack is put into fixer and fixed, timer starts to count
When;
Step 4:After the countdown concluded, staining rack is removed, clear water rinses;
Step 5:Staining rack after the completion of flushing is put into acidolysis in acid hydrolysis solution, and timer starts countdown;
Step 6:Repeat step 4, the staining rack after the completion of flushing, which is put into dyeing liquor, to be dyed, and timer starts countdown;
Step 7:Repeat step 4, the staining rack after the completion of flushing, which is put into rinsing liquid, to be rinsed, and timer starts countdown;
Step 8:After the countdown concluded, staining rack, section dehydration, mounting are taken out.
Wherein:The collocation method of dyeing liquor in step 2, by taking 500ml blender jars as an example, comprise the following steps:
Step a:392ml distilled water is measured in blender jar, reagent A and each 0.4g of reagent B is added, is eventually adding
8ml 5mol/L hydrochloric acid is in blender jar;
Step b:Blender jar is placed in the blender jar putting hole in liquid workspace, and adjusts blender jar by elastic force geometrical clamp
Placing height and fixation, open and are stirred with alloy melt stirring device;
Step c:Funnel and filter paper are placed in another blender jar with liquid workspace;
Step d:The dyeing liquor that step b stirrings are completed is filtered by step c blender jar, completes configuration.
Wherein:Reagent A described in step a is thionine, one kind in reddish black or paramagenta;Described reagent B is burnt sub-
Sodium sulphate or potassium metabisulfite.
Each parameter is respectively in above-described embodiment:
Dyeing temperature described in step 1 is 25 DEG C, 35 DEG C or 55 DEG C;
It is 3min, 5min that step 3, timer described in 5,6,7, which set the time, 10min, 25min, 30min, 40min,
50min, 60min or 75min;
Fixer in step 3 is formaldehyde, two or more composition in ethanol, methanol, glacial acetic acid;
Acid hydrolysis solution described in step 5 is hydrochloric acid solution, concentration 1mol/L, 3mol/L, 5mol/L or 6mol/L;
Rinsing liquid described in step 7 is distilled water or metabisulfite solution.
The colouring method of dyeing pattern shown in Fig. 9 is:
Step 1:Added water into water bath and do not cross Liquid level inductor peak, opening for electronic type time switch is set
Close the time, start preheating, it is 55 DEG C to set dyeing temperature by temperature controller;
Step 2:Dyeing liquor is configured with liquid workspace, carries out with liquid process matching somebody with somebody liquid by above-mentioned, is dyeing the dye of workspace
Ethanol-formaldehyde fixer, 5mol/L acid hydrolysis solutions, the dyeing liquor with the configuration of liquid workspace, distilled water rinsing are added in color cylinder respectively
Liquid;
Step 3:Pathological section is inserted in staining rack, staining rack is put into fixer to fixed, timer timing 3min,
Start countdown;
Step 4:After the countdown concluded, staining rack is removed, clear water rinses;
Step 5:Staining rack after the completion of flushing is put into acidolysis in acid hydrolysis solution, timer timing 5min, starts countdown;
Step 6:Repeat step 4, the staining rack after the completion of flushing, which is put into dyeing liquor, to be dyed, and timer timing 10min, is opened
Beginning countdown;
Step 7:Repeat step 4, the staining rack after the completion of flushing, which is put into rinsing liquid, to be rinsed, and timer timing 3min, is opened
Beginning countdown;
Step 8:After the countdown concluded, staining rack, section dehydration, mounting are taken out.
The colouring method of dyeing pattern shown in Figure 10 is:
Step 1:Added water into water bath and do not cross Liquid level inductor peak, opening for electronic type time switch is set
Close the time, start preheating, it is 35 DEG C to set dyeing temperature by temperature controller;
Step 2:Dyeing liquor is configured with liquid workspace, carries out with liquid process matching somebody with somebody liquid by above-mentioned, is dyeing the dye of workspace
Methanol-formaldehyde-glacial acetic acid fixer, 5mol/L acid hydrolysis solutions, the dyeing liquor with the configuration of liquid workspace, Jiao Ya are added in color cylinder respectively
Sodium sulphate rinsing liquid;
Step 3:Pathological section is inserted in staining rack, staining rack is put into fixer to fixed, timer timing
30min, start countdown;
Step 4:After the countdown concluded, staining rack is removed, clear water rinses;
Step 5:Staining rack after the completion of flushing is put into acidolysis in acid hydrolysis solution, timer timing 25min, starts countdown;
Step 6:Repeat step 4, the staining rack after the completion of flushing, which is put into dyeing liquor, to be dyed, and timer timing 40min, is opened
Beginning countdown;
Step 7:Repeat step 4, the staining rack after the completion of flushing, which is put into rinsing liquid, to be rinsed, and timer timing 5min, is opened
Beginning countdown;
Step 8:After the countdown concluded, staining rack, section dehydration, mounting are taken out.
Although specifically showing and describing the utility model with reference to preferred embodiment, those skilled in the art should
This is understood, is not departing from the spirit and scope of the present utility model that appended claims are limited, in form and details
On the utility model is made a variety of changes, be the scope of protection of the utility model.
Claims (6)
1. mini multifunctional dyeing work station, it is characterised in that including casing, the water bath being embedded in casing, temperature control system
To unite, with alloy melt stirring device, automaton and discharging tube, described water bath is divided into liquid workspace and dyeing workspace,
Described temperature-controlling system controls the temperature with liquid workspace and the temperature of dyeing workspace simultaneously, described to consolidate with alloy melt stirring device
Be scheduled in casing and the water level in the lower section with liquid workspace, automatic control system control water bath, temperature-controlling system, with liquid
Agitating device and the operating time of dyeing workspace, described discharging tube connect with water bath, and the water in water bath passes through discharge opeing
Pipe is discharged.
2. mini multifunctional dyeing work station according to claim 1, it is characterised in that described matches somebody with somebody in liquid workspace
Plate is placed provided with blender jar, blender jar places plate and is provided with least one blender jar putting hole, set around blender jar putting hole
Elastic geometrical clamp, blender jar is placed with blender jar putting hole, and elastic force geometrical clamp fixes blender jar and then controls blender jar
Position height, described is disposed therein the lower section of a blender jar putting hole with alloy melt stirring device, and matches somebody with somebody alloy melt stirring device
Central point and blender jar putting hole the center of circle on coaxial line, it is described with being additionally provided with a lucite above liquid workspace
Cover, the outer surface of described plexiglass tent is coated with preventing water drops holiday layer.
3. mini multifunctional dyeing work station according to claim 2, it is characterised in that described matches somebody with somebody alloy melt stirring device
Including metal cap, two pole pieces, pole piece fixed plate and rotary electric machine, rotary electric machine stretches out a rotary shaft, and pole piece is fixed
Plate is fixed on the rotating shaft, and two pole piece homopolarities are relative and symmetrical and are fixed in pole piece fixed plate, metal
Cover pole piece fixed plate, pole piece and rotary electric machine, described two pole pieces symmetrical central point and blender jar
There is metal stirring sheet in the center of circle of putting hole on coaxial line in the described blender jar being placed in above with alloy melt stirring device, described
Rotated with alloy melt stirring device driving metal stirring sheet and then stir the liquid in blender jar.
4. mini multifunctional dyeing work station according to claim 1, it is characterised in that described temperature-controlling system includes
Electrode, temperature detector and temperature controller are heated, described heating electrode is distributed in water bath, to liquid workspace and dyer
Make area's heating, temperature detector is arranged in water bath, and temperature controller control heating electrode work, temperature detector temperature sensor simultaneously feeds back
To temperature controller, temperature controller and then it is adjusted.
5. mini multifunctional dyeing work station according to claim 1, it is characterised in that described dyeing workspace is set
There are 4 staining jar positions, staining jar is placed in staining jar position, staining rack, described staining jar and staining rack are placed with staining jar
Using PP materials.
6. mini multifunctional dyeing work station according to claim 1, it is characterised in that described automaton
Including Liquid level inductor, electronic type time switch, embedded timer and control circuit, in described embedded timer
On casing, Liquid level inductor is placed in water bath, and electronic type time switch controls the switch of control circuit, control electricity
Road control temperature-controlling system, the operating time with the water level in alloy melt stirring device, water bath and dyeing workspace.
Priority Applications (1)
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CN201720521666.0U CN206710176U (en) | 2017-05-11 | 2017-05-11 | Mini multifunctional dyeing work station |
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CN201720521666.0U CN206710176U (en) | 2017-05-11 | 2017-05-11 | Mini multifunctional dyeing work station |
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CN201720521666.0U Withdrawn - After Issue CN206710176U (en) | 2017-05-11 | 2017-05-11 | Mini multifunctional dyeing work station |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106950094A (en) * | 2017-05-11 | 2017-07-14 | 麦克奥迪(厦门)医疗诊断系统有限公司 | Mini multifunctional dyeing work station and colouring method |
CN114112570A (en) * | 2021-11-12 | 2022-03-01 | 吉林市国科医工科技发展有限公司 | Full-automatic sperm sheet making mechanism based on Diff-Quick staining method |
-
2017
- 2017-05-11 CN CN201720521666.0U patent/CN206710176U/en not_active Withdrawn - After Issue
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106950094A (en) * | 2017-05-11 | 2017-07-14 | 麦克奥迪(厦门)医疗诊断系统有限公司 | Mini multifunctional dyeing work station and colouring method |
CN114112570A (en) * | 2021-11-12 | 2022-03-01 | 吉林市国科医工科技发展有限公司 | Full-automatic sperm sheet making mechanism based on Diff-Quick staining method |
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