CN215492714U - Pathological sample preparation and deoxyribonucleic acid quantitative staining instrument - Google Patents
Pathological sample preparation and deoxyribonucleic acid quantitative staining instrument Download PDFInfo
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- CN215492714U CN215492714U CN202120015146.9U CN202120015146U CN215492714U CN 215492714 U CN215492714 U CN 215492714U CN 202120015146 U CN202120015146 U CN 202120015146U CN 215492714 U CN215492714 U CN 215492714U
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Abstract
The utility model discloses a pathological sample preparation and deoxyribonucleic acid quantitative staining instrument which is characterized by mainly comprising a sample preparation module, a deoxyribonucleic acid quantitative staining module, a temperature control module and a system control module, a waste liquid collecting device, a waste gas collecting device and a waste solid collecting device. The technical scheme can realize the integration of pathological sample preparation and dyeing, is safe and environment-friendly, is convenient to use, covers the whole process, and is flexible in program selection elasticity. Meets the requirements of standardization and automation of a pathology department laboratory.
Description
Technical Field
The utility model relates to the technical field of medical machinery, in particular to a pathological sample preparation and deoxyribonucleic acid quantitative staining instrument.
Background
With the increasing demands of modern industrial development on robots and automated processing, the requirements of various industries on test automation are higher and higher, particularly in medicine and life science. At present, most of the existing quantitative DNA staining is manual operation, or the flaking and the staining are realized by different machines respectively, the whole process is equivalent to semi-automation strictly, the operation is more troublesome, and the cost of manpower is increased. Quantitative dyeing of deoxyribonucleic acid is a specific dyeing method and a special chemical reaction, temperature is a key factor influencing acidolysis, and the dyeing effect of the deoxyribonucleic acid is influenced by insufficient or excessive acidolysis. Therefore, the development of the automatic equipment focuses on temperature control and acid-resistant protection.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
The utility model aims to solve the technical problem of providing a pathological sample preparation and deoxyribonucleic acid quantitative dyeing instrument which can realize the integration of pathological sample preparation and dyeing, is safe and environment-friendly, is convenient and fast to use, covers the whole process and is flexible in program selection elasticity.
(II) technical scheme
In order to achieve the purpose, the technical scheme of the utility model is as follows:
a pathological sample preparation and deoxyribonucleic acid quantitative staining instrument is characterized by mainly comprising a sample preparation module, a deoxyribonucleic acid quantitative staining module, a temperature control module and a system control module. The auxiliary waste collection module consists of a waste liquid collection device, a waste gas collection device and a waste solid collection device.
Furthermore, the sample preparation module comprises a porous sheet making plate, a suction head seat, a centrifuge tube base, a liquid transfer device, a liquid injection needle, a liquid storage tank connected with the liquid injection needle and the like. The slide making plate is used for loading slides and a sedimentation bin, the pipette is used for taking a disposable suction head from the suction head seat by a sample, then filling liquid into the centrifugal tube and sucking a proper amount of sample, and transferring the sample into the sedimentation bin; and the liquid injection needle is used for absorbing and discarding the waste liquid in the sedimentation bin and injecting cleaning liquid to finish flaking.
Furthermore, the quantitative deoxyribonucleic acid staining module comprises a liquid injection needle, a liquid storage tank connected with the liquid injection needle, a liquid injection pump and the like. The liquid injection needle is used for injecting and cleaning a stationary liquid, an acid liquid, a dye solution and distilled water, and the waste liquid is sucked and discarded to finish the dyeing process. The material of the liquid injection needle is hastelloy.
Furthermore, the temperature control module consists of a heating plate, a refrigerating device, a temperature sensor and a temperature adjusting device. The heating plate, the refrigerating device and the temperature sensor are used for keeping the temperature of the dye liquor in the settling bin during dyeing. The temperature adjusting device is used for preheating the temperature of the reagent.
Further, the system control module is composed of a touch screen, a program loaded in the screen and an auxiliary control system. A control system is arranged in the rack, the operation of each electrical component is coordinated and controlled, the states of each parameter of the control system are displayed by a touch screen on the outer surface of the shell, and the parameter adjustment, the instruction sending and the network interconnection are realized.
Further, a power switch is arranged on the side face of the base.
(III) advantageous effects
The utility model has the beneficial effects that:
1) certain corrosion resistance; (volatile BS fixative, corrosive)
2) The temperature control precision is +/-2 ℃;
3) the whole machine has good air tightness; (preventing SO2 from overflowing) has the functions of collecting waste gas and filtering after dyeing;
4) liquid does not drip in the process of liquid transfer;
5) after cleaning, waste liquid needs to be thoroughly absorbed; (avoid the pollution of residual BS to the dye liquor)
6) Liquid sealing treatment: the dyeing needle is subjected to liquid seal storage, so that the situations of pipe blockage and the like are prevented;
7) the slide preparation process is standardized, cells are randomly and uniformly dispersed on a specific circular area of the slide automatically without overlapping, red blood cells, white blood cells, mucus and impurities which obstruct observation are removed, and background information is well stored.
8) Identifying and managing the slide and the reagent bar code, and associating quality control;
9) local and remote quality control and troubleshooting;
10) emergency key-press: in case of emergency, the mechanical emergency key is pressed in time or the power switch is turned off emergently, then the system is started, and the system is automatically reset.
Drawings
FIG. 1 is a schematic view of a dyeing apparatus of the present invention
FIG. 2 is a schematic view of a tableting apparatus of the present invention
FIG. 3 is a graph showing the effects of embodiment 1 of the present invention
FIG. 4 is a diagram illustrating the effects of embodiment 4 of the present invention
Reference numerals: the device comprises a porous sheet making plate 1, a suction head seat 2, a centrifuge tube base 3, a liquid transfer device 4, a liquid injection needle 5, a liquid storage tank 6, a liquid adding pump 7, a heating plate 8, a refrigerating device 9, a temperature sensor 10, a temperature adjusting device 11, a touch screen 12 and a shell 13.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a pathological sample preparation and dna quantitative staining apparatus and 6 embodiments thereof:
a pathological sample preparation and deoxyribonucleic acid quantitative staining instrument is characterized by mainly comprising a sample preparation module, a deoxyribonucleic acid quantitative staining module, a temperature control module and a system control module. The device is attached with a waste liquid collecting device, a waste gas collecting device and a waste solid collecting device. The sample preparation module comprises a porous sheet making plate 1, a suction head seat 2, a centrifuge tube base 3, a liquid transfer device 4, a liquid injection needle 5, a liquid storage tank 6 connected with the liquid injection needle and the like. The slide making plate 1 is used for loading slides and a sedimentation bin, and the liquid moving device 4 is used for taking a disposable suction head from the suction head seat 2 by a sample, filling liquid into a centrifugal tube, sucking a proper amount of sample and transferring the sample into the sedimentation bin; and the liquid injection needle 5 is used for absorbing and discarding the waste liquid in the sedimentation bin, injecting cleaning liquid and finishing flaking. The quantitative deoxyribonucleic acid staining module comprises a liquid injection needle 5, a liquid storage tank 6 connected with the liquid injection needle, a liquid adding pump 7 and the like. The liquid injection needle 5 is used for injecting and cleaning the stationary liquid, the acid liquid, the dye liquid and the distilled water, and the waste liquid is sucked and discarded to finish the dyeing process. The material of the liquid injection needle is hastelloy. The temperature control module is composed of a heating plate 8, a refrigerating device 9, a temperature sensor 10 and a temperature adjusting device 11. The heating plate 1, the refrigerating device 9 and the temperature sensor 10 are used for keeping the temperature of the dye liquor in the settling bin during dyeing. The temperature adjusting device is used for preheating the temperature of the reagent. The system control module is composed of a touch screen 12, a program loaded in the screen and an auxiliary control system. A control system is arranged in the rack to coordinate and control the operation of each electrical component, and a touch screen on the outer surface of the shell 13 displays the states of each parameter of the control system, and adjusts the parameters, sends instructions and is interconnected with a network. And a power switch is arranged on the side surface of the base.
Example 1:
the first mode is as follows: 1 piece preparation and Papanicolaou staining (gradient centrifugation, automatic transfer, natural sedimentation and Papanicolaou staining)
Pretreatment: numbering the specimen and the slide, and oscillating the specimen; adding 4ml of separation solution into a centrifuge tube, and adding 8ml of specimen; 1080r, centrifuging for 2min, and discarding 8ml of upper layer separation liquid; centrifuging again for 2000r for 10min, and pouring out residual liquid; add 1ml buffer and mix well with shaking.
And (3) processing on a machine for flaking: loading on the machine, setting the working temperature of the machine to be 25 ℃, adding 800ul of buffer solution, and uniformly mixing for N times; transferring 800ul of suspension to a settling bin by a mechanical arm, settling for 10 minutes, and absorbing and discarding waste liquid in the settling bin; cleaning with 1ml of cleaning solution for 3 times, standing the cleaning solution for 1 minute for the last time, and sucking away the cleaning solution; washing with 1ml buffer solution for 3 times, removing buffer solution, adding 1ml hematoxylin, and standing for 3 minutes; removing hematoxylin by suction, washing for 2 times by 1ml of buffer solution, and standing for 1 minute by the last buffer solution; absorbing and removing the buffer solution, washing the buffer solution for 2 times, standing for 1 minute for the last time, absorbing and removing, standing for 5 minutes by 1ml of EA staining solution, absorbing and removing the EA staining solution, washing for 2 times by 1ml of washing solution, and standing for 1 minute for the last time, namely absorbing and removing the residual liquid in the settling bin.
Manual sealing: dehydrating with 100% ethanol, transparent xylene, and sealing.
And (3) dyeing results: FIG. 3
Example 2:
and a second mode: 2 pieces are prepared and DNA staining is carried out (gradient centrifugation, automatic transfer, natural sedimentation and DNA staining)
Pretreatment: numbering the specimen and the slide, and oscillating the specimen; adding 4ml of separation solution into a centrifuge tube, and adding 8ml of specimen; 1080r, centrifuging for 2min, and discarding 8ml of upper layer separation liquid; centrifuging again for 2000r for 10min, and pouring out residual liquid; add 1ml buffer and mix well with shaking.
And (3) processing on a machine for flaking: loading on the machine, setting the working temperature of the machine to be 35 ℃, adding 800ul of buffer solution, and uniformly mixing for N times; transferring 800ul of suspension to a settling bin 1 and a settling bin 2 by a mechanical arm, and settling for 10 minutes; absorbing and discarding the waste liquid in the settling bin 1 and the settling bin 2; adding 1ml buffer solution, and removing by suction; 1ml of the cleaning solution was washed 3 times, and the last cleaning solution was left to stand for 1 minute. The cleaning solution is sucked and discarded in the settling bin 2, and 1ml of the stationary liquid in the settling bin 2 is kept stand for 30 minutes; settling a bin 2, sucking and discarding the stationary liquid, and washing 6-8 times with 1ml of distilled water; settling a 2, 1ml hydrolysis solution in a settling bin, and standing for 25 minutes; settling a bin 2, sucking and discarding the hydrolysis solution, and washing for 6-8 times by 1ml of distilled water; settling a bin for 2, 3ml of DNA dye solution to stand for 40 minutes; settling a bin 2, sucking and discarding DNA dye solution, and washing 6-8 times with 1ml of distilled water; settling a bin for 2, 3ml of distilled water to stand for 5 minutes; and residual liquid in the settling bin 1 and the settling bin 2 is sucked and discarded.
Manual sealing: dehydrating with 100% ethanol, transparent xylene, and sealing.
And (3) dyeing results:
example 3:
and a third mode: independent 2 pieces (gradient centrifugation + automatic transfer + natural sedimentation)
Pretreatment: numbering the specimen and the slide, and oscillating the specimen; adding 4ml of separation solution into a centrifuge tube, and adding 8ml of specimen; 1080r, centrifuging for 2min, and discarding 8ml of upper layer separation liquid; centrifuging again for 2000r for 10min, and pouring out residual liquid; add 1ml buffer and mix well with shaking.
And (3) processing on a machine for flaking: loading on a machine, adding 800ul of buffer solution, and uniformly mixing for N times; transferring 800ul of suspension to a settling bin 1 and a settling bin 2 by a mechanical arm, and settling for 10 minutes; absorbing and discarding the waste liquid in the settling bin 1 and the settling bin 2; adding 1ml buffer solution, and removing by suction; washing with 1ml of the washing solution for 3 times, standing the washing solution for 1 minute for the last time, and sucking away the washing solution.
Example 4:
and a fourth mode: 2 pieces are made and Papanicolaou staining is carried out (gradient centrifugation, automatic transfer, natural sedimentation and Papanicolaou staining)
Pretreatment: numbering the specimen and the slide, and oscillating the specimen; adding 4ml of separation solution into a centrifuge tube, and adding 8ml of specimen; 1080r, centrifuging for 2min, and discarding 8ml of upper layer separation liquid; centrifuging again for 2000r for 10min, and pouring out residual liquid; add 1ml buffer and mix well with shaking.
And (3) processing on a machine for flaking: loading on the machine, setting the working temperature of the machine to be 25 ℃, adding 800ul of buffer solution, and uniformly mixing for N times; transferring 800ul of suspension to a settling bin 1 and a settling bin 2 by a mechanical arm, and settling for 10 minutes; absorbing and discarding the waste liquid in the settling bin 1 and the settling bin 2; adding 1ml buffer solution, and removing by suction; 1ml of the cleaning solution was washed 3 times, and the last cleaning solution was left to stand for 1 minute. The cleaning solution is sucked and discarded in the settling bin 1, and 1ml of buffer solution is washed for 3 times; the buffer solution is sucked and removed from the settling bin 1, and 1ml of hematoxylin is kept stand for 3 minutes; the hematoxylin is sucked and removed from the settling bin 1, 1ml of buffer solution is used for cleaning for 3 times, and the last time of buffer solution is kept stand for 1 minute; the buffer solution is sucked and discarded in the settling bin 1, 1ml of cleaning solution is washed for 2 times, and the buffer solution is kept stand for 1 minute for the last time; the cleaning solution is sucked and discarded in a settling bin 1, and 1ml of EA staining solution is kept stand for 5 minutes; absorbing EA staining solution in a settling bin 1, washing for 3 times by 1ml of cleaning solution, and standing for 1 minute for the last time; and residual liquid in the settling bin 1 and the settling bin 2 is sucked and discarded.
Manual sealing: dehydrating with 100% ethanol, transparent xylene, and sealing.
And (3) dyeing results: FIG. 4
Example 5:
and a fifth mode: independent 1 sheet (gradient centrifugation + automatic transfer + natural sedimentation)
Pretreatment: numbering the specimen and the slide, and oscillating the specimen; adding 4ml of separation solution into a centrifuge tube, and adding 8ml of specimen; 1080r, centrifuging for 2min, and discarding 8ml of upper layer separation liquid; centrifuging again for 2000r for 10min, and pouring out residual liquid; add 1ml buffer and mix well with shaking.
And (3) processing on a machine for flaking: loading on a machine, adding 800ul of buffer solution, and uniformly mixing for N times; transferring 800ul of suspension to a settling bin by a mechanical arm, and settling for 10 minutes; absorbing and discarding the waste liquid in the settling bin; adding 1ml buffer solution, and removing by suction; washing with 1ml of the washing solution for 3 times, standing the washing solution for 1 minute for the last time, and sucking away the washing solution.
Example 6:
mode six: preparation of 1 piece + DNA staining (gradient centrifugation + automatic transfer + Natural sedimentation + DNA staining)
Pretreatment: numbering the specimen and the slide, and oscillating the specimen; adding 4ml of separation solution into a centrifuge tube, and adding 8ml of specimen; 1080r, centrifuging for 2min, and discarding 8ml of upper layer separation liquid; centrifuging again for 2000r for 10min, and pouring out residual liquid; add 1ml buffer and mix well with shaking.
And (3) processing on a machine for flaking: loading on the machine, setting the working temperature of the machine to be 25 ℃, adding 800ul of buffer solution, and uniformly mixing for N times; transferring 800ul of suspension to a settling bin by a mechanical arm, and settling for 10 minutes; absorbing and discarding the waste liquid in the settling bin; adding 1ml buffer solution, and removing by suction; 1ml of the cleaning solution was washed 3 times, and the last cleaning solution was left to stand for 1 minute. The cleaning solution is sucked and discarded in a settling bin, and 1ml of stationary liquid is kept stand for 50 minutes in the settling bin; settling a bin, sucking and discarding the stationary liquid, and washing 6-8 times with 1ml of distilled water; settling a bin, and standing 1ml of hydrolysis solution for 60 minutes; settling a bin, sucking and discarding the hydrolysis solution, and washing 6-8 times with 1ml of distilled water; settling a bin, and standing 3ml of DNA dye solution for 75 minutes; settling a bin, sucking and discarding DNA dye solution, and washing 6-8 times with 1ml of distilled water; settling a bin, and standing 3ml of distilled water for 15 minutes; and (5) absorbing and discarding the residual liquid in the settling bin.
Manual sealing: dehydrating with 100% ethanol, transparent xylene, and sealing.
And (3) dyeing results:
| specimen number | IOD | CV |
| HEC 200201 | 117 | 3.6 |
| HEC 200202 | 122 | 2.8 |
| HEC 200203 | 135 | 3.1 |
| HEC 200204 | 126 | 2.7 |
| HEC 200205 | 127 | 3.0 |
| HEC 200206 | 117 | 3.4 |
| HEC 200207 | 125 | 3.9 |
In addition to the above procedures, the method can also be used for other program settings of biological tissue staining.
While the utility model has been particularly shown and described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the utility model as defined by the appended claims.
Claims (5)
1. A pathological sample preparation and deoxyribonucleic acid quantitative staining instrument is characterized by comprising a sample preparation module, a deoxyribonucleic acid quantitative staining module, a temperature control module, a system control module and a waste collection module, wherein the deoxyribonucleic acid quantitative staining module comprises a liquid injection needle, a liquid storage tank connected with the liquid injection needle and a liquid injection pump; the liquid injection needle is used for injecting and cleaning the stationary liquid, the acid liquid, the dye liquid and the distilled water, and the waste liquid is sucked and discarded to finish the dyeing process; the material of the liquid injection needle is hastelloy.
2. The pathological sample preparation and DNA quantitative staining instrument of claim 1, wherein: the waste collection module consists of a waste liquid collection device, a waste gas collection device and a waste solid collection device.
3. The pathological sample preparation and DNA quantitative staining instrument of claim 1, wherein: the sample preparation module consists of a porous sheet making plate, a suction head seat, a centrifuge tube base, a liquid transfer device, a liquid injection needle and a liquid storage tank connected with the liquid injection needle; the slide making plate is used for loading slides and a sedimentation bin, the pipette is used for taking a disposable suction head from the suction head seat by a sample, then filling liquid into the centrifugal tube and sucking a proper amount of sample, and transferring the sample into the sedimentation bin; and the liquid injection needle is used for absorbing and discarding the waste liquid in the sedimentation bin and injecting cleaning liquid to finish flaking.
4. The pathological sample preparation and DNA quantitative staining instrument of claim 1, wherein: the temperature control module consists of a heating plate, a refrigerating device, a temperature sensor and a temperature adjusting device; the heating plate, the refrigerating device and the temperature sensor are used for keeping the temperature of the dye liquor in the settling bin during dyeing; the temperature adjusting device is used for preheating the temperature of the reagent.
5. The pathological sample preparation and DNA quantitative staining instrument of claim 1, wherein: a control system is arranged in the rack, the operation of each electrical component is coordinated and controlled, the states of each parameter of the control system are displayed by a touch screen on the outer surface of the shell, and the parameter adjustment, the instruction sending and the network interconnection are realized.
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| CN202120015146.9U CN215492714U (en) | 2021-01-05 | 2021-01-05 | Pathological sample preparation and deoxyribonucleic acid quantitative staining instrument |
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