CN104777026A - Liquid-dropping type automatic biological staining instrument - Google Patents
Liquid-dropping type automatic biological staining instrument Download PDFInfo
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- CN104777026A CN104777026A CN201410016181.7A CN201410016181A CN104777026A CN 104777026 A CN104777026 A CN 104777026A CN 201410016181 A CN201410016181 A CN 201410016181A CN 104777026 A CN104777026 A CN 104777026A
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Abstract
The invention relates to a liquid-dropping type automatic biological staining instrument; the top end of a machine frame is provided with a loading port; a vertical track extends downward to the interior of the machine frame from the loading port; a slide box is arranged at the loading port, can rise and fall up and down along the vertical track, and is internally provided with slides for bacteria staining; a transverse track is transversely arranged inside the machine frame; the rear end of a manipulator is in sliding connection on the transverse track, and the front end of the manipulator is used for clamping the slides in the slide box; a liquid-dropping type staining and cleaning device is arranged at the top end of the machine frame; a drying device is arranged inside the machine frame; a waste liquid cup is arranged at the bottom end of the machine frame and is used for containing a waste liquid of a staining liquid and a cleaning waste liquid; a control module is arranged on the side face of the machine frame, and is electrically connected with the manipulator, the liquid-dropping type staining and cleaning device and the drying device. Compared with the prior art, the liquid-dropping type automatic biological staining instrument has the advantages of simple operation, reliable method and stable performance, and can meet various staining ways.
Description
Technical field
The present invention relates to a kind of biological stain instrument, especially relate to a kind of dribble dropping automated biological staining instruments.
Background technology
Dyeing is the means understanding bacterium and cellular morphology, and removing from office blue, antiacid and blood cell staining is main and widely used method, is mainly manual operation at present, Grain stain and Ziehi-Neelsen stain is summarized as follows respectively:
Gram staining is founded by Denmark doctor Gram for 1884.Bacterium is first dyeed through basic-dyeable fibre crystallization, again after the mordant dyeing of iodine liquid, decolour with alcohol, the bacterium purple had under certain condition is not divested, what have is divested, therefore bacterium can be divided into two large classes, the former is called gram-positive bacteria (G+), and the latter is Gram-negative bacteria (G-).For observing conveniently, red etc. to redye with a kind of orchil is as luxuriant in alkalescence again after decolouring.Positive bacteria is still with purple, and negative bacterium is then by red-dyed.Have the bacillus of gemma and most coccus, and all defence line bacterium and fungi are all gram positive reaction; Vibrios, conveyor screw and most of pathogenic sporeless bacterium all present negative reaction.
Gram-positive bacteria and Gram-negative bacteria have a lot of difference in chemical composition and physiological property, and staining reaction is different.It is generally acknowledged now that Gram-positive thalline includes special nucleoprotein magnesium salts and the compound of polysaccharide, it is combined very firm with the compound of iodine and crystal violet, not easily decolours, negative bacterium compound combination degree is low, absorbing dye is poor, easily decolours, and this is the Main Basis of staining reaction.In addition, positive bacteria thalline isoelectric point comparatively negative bacterium is low, dyes under identical PH condition, and positive bacteria absorption basic-dyeable fibre is a lot, and therefore not easily slough, negative bacterium is then contrary.So condition during dyeing will strictly control.Such as, dye under the condition of highly basic, two class bacterium absorption basic-dyeable fibres are all many, can be all positive reaction; When PH is very low, then can be all negative reaction.In addition, the cell membrane of two class bacterium etc. are also inconsistent to the permeability of crystal violet-Surgidine, and positive bacteria permeability is little, therefore is not easily decoloured, and negative bacterium permeability is large, easily decolours.So bleaching time.Discoloration method also should strictly control.
Disscusion about Gram stain principle is as follows:
G+ bacterium: cell wall thickness, peptide glycan network molecule forms a kind of permeability barrier, and when ethanol decolorization, peptide glycan dewaters and apodization reduces, therefore retains crystal violet-Surgidine on cell membrane.In purple.
G-bacterium: peptidoglycan layer is thin, crosslinked loose, ethanol decolorization can not make its structure both shrinks, and its fat content is high, and ethanol is by liposoluble solution, and gap is strengthened, and crystal violet-Surgidine stripping cell membrane, husky Huang takes on a red color after redying.
Gram's stain generally comprises just dye, mordant dyeing, decolour, four steps such as to redye, concrete operation method is: (1) smear is fixed, (2) ammonium oxalate crystal violet contaminates 1 minute, (3) tap water, (4) add iodine liquid covering painting face and contaminate 1 minute, (5) wash, suck moisture with thieving paper, (6) add 95% alcohol number and drip, and shake is decoloured gently, 30 seconds after washings, suck moisture, after (7) luxuriant red colouring liquid (rare) (or husky yellow) contaminated for 10 seconds, tap water, (8) dry, microscopy.
The result of dyeing, gram positive bacteria dye is in purple, and gram-negative bacteria dye is in red.
Be more than the Grain stain of conventional manual be four steps dyeing and four washings, it operate more complicated, waste water and the time long.Modern Laboratory is busy with one's work high with cost, every technical method is had to the requirement made every effort to easy and save, to complete more task within limited time and cost.
Ziehi-Neelsen stain is initiated and the bacterial stain improving through F. Qi Er (Ziehl) and create by Paul Ehrlich (F.Ehrlich) for 1882.Wherein most is representational is Qi Er-Nelson (Ziehl-Neelsen) decoration method to tulase and Qi Er-Jia Beite (Ziehl-Gabbet) decoration method.After carbolfuchsin dyeing, with acidic alcohol decolouring, then carry out counterstain with methylene blue.
The principle of acid-fast stain is as follows:
Mycobacterium, contains a large amount of lipids containing in the cell membrane of tulase, is enclosed in the outside of peptide glycan, so the general not easy coloring of mycobacterium, it be impelled painted through heating and prolongation dyeing time.But after the mycolic acid in mycobacterium is combined with dyestuff, be just difficult to by acid bleaching agent bleaching, therefore named acid-fast stain.
Together-Nissl Ziehi-Neelsen stain makes mycolic acid become compound with carbolfuchsin strong bonded in a heated condition, with hydrochloride alcohol process also nondiscoloration.When adding after alkaline methylene blue redyes again, mycobacterium is still red, and the material in other bacteriums and background be blueness.
Acid-fast stain general step is as follows:
1) just contaminate: clamp smear preparation with glass slide clamp, drip carbolfuchsin 2-3 and drip, in the heating slowly of flame eminence, be sure not boiling, there is steam and away from keyboard, if dye liquor evaporation reduces, dye liquor should be added again, in order to avoid dry, heat 5 minutes, rinse with water after sample cooling.
2) decolour: 3% hydrochloride alcohol decolours 3 minutes; Rinse with water.
3) redye: redye 30 seconds with alkaline methylene blue solution, washing, blot with thieving paper and use oily sem observation afterwards.
Be more than conventional artificial acid-fast stain be three steps dyeing and three washings, it operate more complicated, waste water and the time long.Modern Laboratory is busy with one's work high with cost, every technical method is had to the requirement made every effort to easy and save, to complete more task within limited time and cost.
Chinese patent CN102220236A discloses a kind of automatic staining instrument with Gram staining two-step method, this dyeing instrument is made up of color pond, service sink, the dry pond of current of warm air, mechanical arm, track and display screen, color pond has two, the dry pond of first color pond, the second color pond, service sink and current of warm air is arranged in order the front portion being arranged on dyeing instrument, mechanical arm is located at the top of dyeing instrument, can place in the front end of this mechanical arm and treat dye sheet or Quality Control sheet, the rear end of mechanical arm is connected with described track, and display screen is located at the side at dyeing instrument top.Simply, to water quality without particular/special requirement, produce and drop into without poisonous and harmful substances in checkout procedure and produce, provide quicker, easy standardized experimental result, result is easy to observe and judgement, safe and reliable for this invention production technology and operating process.But what adopt due to this dyeing instrument is put into the mode that color pond contaminates, and causes Color bad.
Summary of the invention
Object of the present invention is exactly providing to overcome defect that above-mentioned prior art exists that a kind of easy and simple to handle, method is reliable, stable performance, can meet the dribble dropping automated biological staining instruments of multiple staining method.
Object of the present invention can be achieved through the following technical solutions:
A kind of dribble dropping automated biological staining instruments, comprise frame, vertical track, slide box, cross track, mechanical arm, dribble dropping dyeing and cleaning device, baker, waste liquid cup and control module, the top of described frame is provided with a load port, described vertical track reaches machine frame inside downwards from load port, described slide box is located at load port place, and can vertically track oscilaltion, slide box is built with bacterium or cell dyeing slide, described cross track is laterally located at the inside of frame, the rear end of described mechanical arm is slidably connected on cross track, the front end of mechanical arm is for clamping the slide in slide box, described dribble dropping dyeing and cleaning device are located at the top of frame, described baker is located at machine frame inside, described waste liquid cup is located at the bottom of frame, for splendid attire dye liquor waste liquid and cleaning waste liquid, described control module is located on the side of frame, and and mechanical arm, dribble dropping dyeing is electrically connected with cleaning device and baker,
Slide box vertically track declines, mechanical arm clamps out slide in slide box, and on cross track transverse shifting, by the below of dribble dropping dyeing with cleaning device, dribble dropping dyeing and cleaning device carry out dyeing to slide and clean, and then mechanical arm sends slide back to slide box, get next slide and continue same action, after all Coplin complete, baker is dried the slide in slide box, carries out microscopy after drying.
Described dribble dropping dyeing comprises peristaltic pump and reagent bottle frame with cleaning device, described peristaltic pump is provided with several, be laid in the top of frame, described reagent bottle frame is located at the bottom of frame, for placing just stain storage bottle, counterstain storage bottle and cleaning fluid storage bottle, woven hose one end on peristaltic pump extend into just in stain storage bottle, counterstain storage bottle or cleaning fluid storage bottle, and the other end is injected into machine frame inside, by the mode of dropping liquid to Coplin and cleaning; Be provided with just stain in described first stain storage bottle, be provided with counterstain in described counterstain storage bottle, in described cleaning fluid storage bottle, be provided with cleaning fluid.
Described peristaltic pump is at least provided with 3, is respectively used to carry just stain, counterstain and cleaning fluid.
Described peristaltic pump can be provided with multiple, arbitrarily angledly all can vertically spray dye liquor or cleaning fluid to slide bilateral.
Described waste liquid cup draw and insert-type is connected to the bottom of frame, can depart from frame.
Described mechanical arm can level or vertical mobile or rotation.
The operation interface that described control module comprises control circuit board and is connected with control circuit board.
Slide 1 ~ 30 is provided with in described slide box.
Be provided with in described slide box slide 5,10 or 20.
The bake out temperature of described baker is located at 40 ~ 60 DEG C.
When using instrument of the present invention to dye, the material to be checked on slide is chosen as the bacterium of cultivation, blood, phlegm, just or secretion etc.
Dribble dropping automated biological staining instruments of the present invention can optionally antiacid or fluorescence TB or Grain stain.
When using instrument of the present invention to carry out acid-fast stain, the first first stain (solution containing 2.0% basic fuchsin) dripping 1-3ml one step ahead covers sample, dyeing 2-3 minute, washing, then drip 3.0% hydrochloride alcohol, decolour 30 seconds, washing, counterstain (the methylene blue solution of 0.8%) 1-3ml covers sample, dyes 20-30 second, washing, microscopy after dry.Can see in smear, dye red for antiacid positive bacteria with what dye blue look is antiacid negative bacterium, with distinct contrast.
When using instrument of the present invention to carry out Grain stain, the first stain (containing 0.5% crystal violet solution) first dripping 1-3ml covers sample, dye 30 seconds, washing, then drip 1-3ml mordant dyeing decolorant (iodine of 1% and alcohol acetone soln) and cover sample, 10 seconds, washing, finally drips the basic fuchsin solution of 0.6%, redyes 10 seconds, washing, microscopy after dry.Can see in smear, the purple dyed is gram positive bacteria and to dye red be gram-negative bacteria, with distinct contrast.
When using instrument of the present invention to carry out fluorescence TB dyeing, the first stain (containing 0.75% acridine alcoholic solution) first dripping 1-3ml covers sample, dyes 15 minutes, washing; Drip decolorant (hydrochloride alcohol solution of 3%) 1-3ml again and cover sample, decolour 30 seconds, washing; Finally redye dropping 1-3ml counterstain (liquor potassic permanganate of 0.5%) to decolour 30 seconds, washing, microscopy after dry.Can see in smear, the bright crocus dyed is that TB is positive and background is colourless, with distinct contrast.
When using instrument of the present invention to carry out blood cell staining, the first stain (containing 2% Rui Shi-Giemsa solution) first dripping 1-3ml covers sample, dyes 1 minute, washing; Drip buffering agent (phosphate solution of 0.2%) 1-3ml again and cover sample, 120 seconds, washing; Microscopy after dry.Can see in smear, the tenuigenin of the redness dyed and the nucleus of purple, with distinct contrast.
Compared with prior art, the present invention has the following advantages and beneficial effect:
1, this dribble dropping automated biological staining instruments, can dye to 20 or more samples at every turn, for automatic staining (as antiacid, leather is blue and blood cell staining etc.) method of two kinds or more.Compared with existing method (artificial or instrument), the invention provides quicker, reliable, efficient, multi-functional and easy standardized method;
2, use production technology of the present invention and operating process simply, to water quality without particular/special requirement, produce and drop into without poisonous and harmful substances in checkout procedure and produce;
3, the present invention can provide quicker, easy standardized experimental result, microscopy after automatic staining, can see the bacterium of dyeing in smear central authorities;
4, the present invention integrates dyeing, cleaning, drying; Most multipotency per hour contaminates 100, short run dye sheet in enormous quantities all can, efficiency is higher, easy and simple to handle, friendly interface, result are easy to observe and judge;
5, sample presentation is easy, and namely sample arrives and namely do, and continuity is strong, and the intellectualized operation of employing can sample storage automatically;
6, the present invention is conducive to biology or medical practice, is conducive to commercialization transport, and safe and reliable, has very large potential market, can produce significantly society and economic benefit;
7, peristaltic pump can be provided with multiple, arbitrarily angledly all can vertically spray water by bilateral;
8, each parts Clear partition, is convenient on shell, arrange access hole and topple over waste liquid.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
In figure, 1 is frame, and 2 is vertical track, and 3 is slide box, and 4 is cross track, and 5 is mechanical arm, and 6 is peristaltic pump, and 7 is reagent bottle frame, and 8 is baker, and 9 is waste liquid cup, and 10 is control module
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in detail.
Embodiment
A kind of dribble dropping automated biological staining instruments, as shown in Figure 1, comprise frame 1, vertical track 2, slide box 3, cross track 4, mechanical arm 5, dribble dropping dyeing and cleaning device, baker 8, waste liquid cup 9 and control module 10, the top of frame 1 is provided with a load port, it is inner that vertical track 2 reaches frame 1 downwards from load port, slide box 3 is located at load port place, and can vertically track 2 oscilaltion, slide box 3 is built with Bacterial stain slide, cross track 4 is laterally located at the inside of frame 1, the rear end of mechanical arm 5 is slidably connected on cross track 4, the front end of mechanical arm 5 is for clamping the slide in slide box 3, dribble dropping dyeing and cleaning device are located at the top of frame 1, it is inner that baker 8 is located at frame 1, waste liquid cup 9 is located at the bottom of frame 1, for splendid attire dye liquor waste liquid and cleaning waste liquid, control module 10 is located on the side of frame 1, and with mechanical arm 5, dribble dropping dyeing is electrically connected with cleaning device and baker 8,
Slide box 3 vertically track 2 declines, mechanical arm 5 clamps out slide in slide box 3, and on cross track 4 transverse shifting, by the below of dribble dropping dyeing with cleaning device, dribble dropping dyeing and cleaning device carry out dyeing to slide and clean, and then mechanical arm 5 sends slide back to slide box 3, get next slide and continue same action, after all Coplin complete, the slide in baker 8 pairs of slide boxs 3 is dried, and carries out microscopy after drying.
Dribble dropping dyeing comprises peristaltic pump 6 and reagent bottle frame 7 with cleaning device, peristaltic pump 6 is provided with several, be laid in the top of frame 1, reagent bottle frame 7 is located at the bottom of frame 1, for placing just stain storage bottle, counterstain storage bottle and cleaning fluid storage bottle, woven hose one end on peristaltic pump 6 extend into just in stain storage bottle, counterstain storage bottle or cleaning fluid storage bottle, and it is inner that the other end is injected into frame 1, by the mode of dropping liquid to Coplin and cleaning; Be provided with just stain in first stain storage bottle, be provided with counterstain in counterstain storage bottle, in cleaning fluid storage bottle, be provided with cleaning fluid.Peristaltic pump 6 is at least provided with 3, is respectively used to carry just stain, counterstain and cleaning fluid.Peristaltic pump 6 can be provided with multiple, arbitrarily angledly all can vertically spray dye liquor or cleaning fluid to slide bilateral.Waste liquid cup 9 draw and insert-type is connected to the bottom of frame 1, can depart from frame 1.Mechanical arm 5 can level or vertical mobile or rotation.The operation interface that control module 10 comprises control circuit board and is connected with control circuit board.Slide 1 ~ 30 can be provided with in slide box 3.Generally be provided with slide 5,10 or 20.The bake out temperature of baker 8 is located at 40 ~ 60 DEG C.
When using instrument of the present invention to dye, the material to be checked on slide is chosen as the bacterium of cultivation, blood, phlegm, just or secretion etc.
Dribble dropping automated biological staining instruments of the present invention can optionally antiacid or fluorescence TB or Grain stain.
When using instrument of the present invention to carry out acid-fast stain, the first first stain (solution containing 2.0% basic fuchsin) dripping 1-3ml one step ahead covers sample, dyeing 2-3 minute, washing, then drip 3.0% hydrochloride alcohol, decolour 30 seconds, washing, counterstain (the methylene blue solution of 0.8%) 1-3ml covers sample, dyes 20-30 second, washing, microscopy after dry.Can see in smear, dye red for antiacid positive bacteria with what dye blue look is antiacid negative bacterium, with distinct contrast.
When using instrument of the present invention to carry out Grain stain, the first stain (containing 0.5% crystal violet solution) first dripping 1-3ml covers sample, dye 30 seconds, washing, then drip 1-3ml mordant dyeing decolorant (iodine of 1% and alcohol acetone soln) and cover sample, 10 seconds, washing, finally drips the basic fuchsin solution of 0.6%, redyes 10 seconds, washing, microscopy after dry.Can see in smear, the purple dyed is gram positive bacteria and to dye red be gram-negative bacteria, with distinct contrast.
When using instrument of the present invention to carry out fluorescence TB dyeing, the first stain (containing 0.75% acridine alcoholic solution) first dripping 1-3ml covers sample, dyes 15 minutes, washing; Drip decolorant (hydrochloride alcohol solution of 3%) 1-3ml again and cover sample, decolour 30 seconds, washing; Finally redye dropping 1-3ml counterstain (liquor potassic permanganate of 0.5%) to decolour 30 seconds, washing, microscopy after dry.Can see in smear, the bright crocus dyed is that TB is positive and background is colourless, with distinct contrast.
When using instrument of the present invention to carry out blood cell staining, the first stain (containing 2% Rui Shi-Giemsa solution) first dripping 1-3ml covers sample, dyes 1 minute, washing; Drip buffering agent (phosphate solution of 0.2%) 1-3ml again and cover sample, 120 seconds, washing; Microscopy after dry.Can see in smear, the tenuigenin of the redness dyed and the nucleus of purple, with distinct contrast.
Claims (8)
1. a dribble dropping automated biological staining instruments, it is characterized in that, comprise frame, vertical track, slide box, cross track, mechanical arm, dribble dropping dyeing and cleaning device, baker, waste liquid cup and control module, the top of described frame is provided with a load port, described vertical track reaches machine frame inside downwards from load port, described slide box is located at load port place, and can vertically track oscilaltion, slide box is built with bacterium or cell dyeing slide, described cross track is laterally located at the inside of frame, the rear end of described mechanical arm is slidably connected on cross track, the front end of mechanical arm is for clamping the slide in slide box, described dribble dropping dyeing and cleaning device are located at the top of frame, described baker is located at machine frame inside, described waste liquid cup is located at the bottom of frame, for splendid attire dye liquor waste liquid and cleaning waste liquid, described control module is located on the side of frame, and and mechanical arm, dribble dropping dyeing is electrically connected with cleaning device and baker,
Slide box vertically track declines, mechanical arm clamps out slide in slide box, and on cross track transverse shifting, by the below of dribble dropping dyeing with cleaning device, dribble dropping dyeing and cleaning device carry out dyeing to slide and clean, and then mechanical arm sends slide back to slide box, get next slide and continue same action, after all Coplin complete, baker is dried the slide in slide box, carries out microscopy after drying.
2. a kind of dribble dropping automated biological staining instruments according to claim 1, it is characterized in that, described dribble dropping dyeing comprises peristaltic pump and reagent bottle frame with cleaning device, described peristaltic pump is provided with several, be laid in the top of frame, described reagent bottle frame is located at the bottom of frame, for placing just stain storage bottle, counterstain storage bottle and cleaning fluid storage bottle, woven hose one end on peristaltic pump extend in first stain storage bottle, counterstain storage bottle or cleaning fluid storage bottle, the other end is injected into machine frame inside, by the mode of dropping liquid to Coplin and cleaning; Be provided with just stain in described first stain storage bottle, be provided with counterstain in described counterstain storage bottle, in described cleaning fluid storage bottle, be provided with cleaning fluid.
3. a kind of dribble dropping automated biological staining instruments according to claim 2, it is characterized in that, described peristaltic pump is at least provided with 3, is respectively used to carry just stain, counterstain and cleaning fluid.
4. a kind of dribble dropping automated biological staining instruments according to claim 1, is characterized in that, described waste liquid cup draw and insert-type is connected to the bottom of frame, can depart from frame.
5. a kind of dribble dropping automated biological staining instruments according to claim 1, is characterized in that, described mechanical arm can level or vertical mobile or rotation.
6. a kind of dribble dropping automated biological staining instruments according to claim 1, is characterized in that, the operation interface that described control module comprises control circuit board and is connected with control circuit board.
7. a kind of dribble dropping automated biological staining instruments according to claim 1, is characterized in that, be provided with slide 1 ~ 30 in described slide box.
8. a kind of dribble dropping automated biological staining instruments according to claim 1, is characterized in that, be provided with in described slide box slide 5,10 or 20.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105241726A (en) * | 2015-09-14 | 2016-01-13 | 黄小军 | Immunohistochemical staining method and applications of immunohistochemical staining method in cervical tumorous lesion screening |
| CN105890955A (en) * | 2016-06-12 | 2016-08-24 | 李源 | Automatic sputum specimen smearing instrument |
| CN106053167A (en) * | 2016-05-19 | 2016-10-26 | 四川金域医学检验中心有限公司 | Preparation method of marrow fluid smear |
| CN106092892A (en) * | 2016-08-12 | 2016-11-09 | 关慧娟 | Crushing type automatic slide finder |
| CN107478485A (en) * | 2017-06-30 | 2017-12-15 | 严铭 | Gram's staining instrument |
| CN107926766A (en) * | 2017-10-17 | 2018-04-20 | 杭州晶雁电子科技有限公司 | A kind of method automatically moved |
-
2014
- 2014-01-14 CN CN201410016181.7A patent/CN104777026A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105241726A (en) * | 2015-09-14 | 2016-01-13 | 黄小军 | Immunohistochemical staining method and applications of immunohistochemical staining method in cervical tumorous lesion screening |
| CN106053167A (en) * | 2016-05-19 | 2016-10-26 | 四川金域医学检验中心有限公司 | Preparation method of marrow fluid smear |
| CN105890955A (en) * | 2016-06-12 | 2016-08-24 | 李源 | Automatic sputum specimen smearing instrument |
| CN105890955B (en) * | 2016-06-12 | 2018-06-19 | 李源 | Sputum specimen automatic smear instrument |
| CN106092892A (en) * | 2016-08-12 | 2016-11-09 | 关慧娟 | Crushing type automatic slide finder |
| CN107478485A (en) * | 2017-06-30 | 2017-12-15 | 严铭 | Gram's staining instrument |
| CN107926766A (en) * | 2017-10-17 | 2018-04-20 | 杭州晶雁电子科技有限公司 | A kind of method automatically moved |
| CN107926766B (en) * | 2017-10-17 | 2023-07-14 | 杭州晶雁电子科技有限公司 | Automatic moving method |
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Application publication date: 20150715 |