CN102220236A - Automatic staining instrument with Gram staining two-step method - Google Patents
Automatic staining instrument with Gram staining two-step method Download PDFInfo
- Publication number
- CN102220236A CN102220236A CN2010101484403A CN201010148440A CN102220236A CN 102220236 A CN102220236 A CN 102220236A CN 2010101484403 A CN2010101484403 A CN 2010101484403A CN 201010148440 A CN201010148440 A CN 201010148440A CN 102220236 A CN102220236 A CN 102220236A
- Authority
- CN
- China
- Prior art keywords
- mechanical manipulator
- pond
- step method
- staining
- instrument
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to an automatic staining instrument with a Gram staining two-step method. The staining instrument comprises two staining pools, a cleaning pool, a hot air flow drying pool, a manipulator, a rail and a display screen, wherein the first staining pool, the second staining pool, the cleaning pool and the hot air flow drying pool are sequentially arranged at the front part of the staining instrument, the manipulator is arranged at the upper part of the staining instrument, slices to be stained or quality control slices can be placed in the front end of the manipulator, the back end of the manipulator is connected with the rail, and the display screen is arranged at one side of the top of the staining instrument. Compared with the prior art, the instrument has the advantages that the protective technology and the operation flow are simple, special requirements to water quality are free, no harmful organisms are introduced and produced during the production and detection processes, more quick and easily-standardized experiment results can be provided, the result is easy to observe and judge, and the safety and reliability are provided.
Description
Technical field
The present invention relates to a kind of automatic staining instrument, especially relate to a kind of gram stain two-step method automatic staining instrument.
Background technology
Gram staining is widely used a kind of differential staining in the bacteriology, is founded by Denmark doctor Gram in 1884.Bacterium is dyeed through the basic dyestuff crystallization earlier, and after the mordant dyeing of iodine liquid, decolours with alcohol, this look of the bacterium that has is not under certain condition sloughed, and what have can be sloughed, and therefore can be divided into two big classes to bacterium, the former is called gram-positive microorganism (G+), and the latter is Gram-negative bacteria (G-).For observing conveniently, after the decolouring again with luxuriant red etc. the redying of a kind of orchil such as alkalescence.Positive bacteria still is with purple, and negative bacterium is then by red-dyed.Bacillus and most coccus that gemma is arranged, and all defence line bacterium and fungi all are the gram positive reaction; Vibrios, spirochete and most of pathogenic sporeless bacteriums all present negative reaction.
Gram-positive microorganism and Gram-negative bacteria have a lot of difference on chemical constitution and physiological property, staining reaction is different.It is generally acknowledged that now the Gram-positive thalline contains the special nucleoprotein magnesium salts and the mixture of polysaccharide, it combines very firm with the mixture of iodine and Viola crystallina, is difficult for decolouring, negative bacterium mixture combination degree is low, absorbing dye is poor, easily decolouring, and this is the main foundation of staining reaction.In addition, positive bacteria thalline iso-electric point is low than negative bacterium, dyes under identical PH condition, and positive bacteria absorption basic dyestuff is a lot, therefore is difficult for sloughing, and negative bacterium is then opposite.So the condition during dyeing is wanted strict control.For example, dye under the alkaline condition, two class bacterium absorption basic dyestuff is all many, all can be positive reaction; When PH is very low, then can all be negative reaction.In addition, the cell walls of two class bacterium etc. are also inconsistent to the permeability of Viola crystallina-Surgidine, and the saturating property of positive bacteria is little, so be difficult for being decoloured, the saturating property of negative bacterium is big, easily decolouring.So bleaching time.Decoloring method also should strict control.
Disscusion about Gram stain principle:
The G+ bacterium: cell wall thickness, the peptidoglycan network molecule forms a kind of permeability barrier, and when ethanol decolorization, peptidoglycan dehydration and apodization dwindles is so reservation Viola crystallina-Surgidine is on cytolemma.Be purple.
The G-bacterium: the peptidoglycan layer is thin, and is crosslinked loose, and ethanol decolorization can not make its structure shrink, its fat content height, and ethanol is separated liposoluble, and the slit is strengthened, and Viola crystallina-Surgidine stripping cell walls takes on a red color after husky Huang is redyed.
Gram staining method generally comprises just dyes, mordant dyeing, four steps such as decolours, redyes, and concrete operation method is:
1) smear is fixed.
2) ammonium oxalate crystal violet dyed 1 minute.
3) tap water flushing.
4) adding iodine liquid covers and to be coated with face and to dye 1 minute.
5) washing is inhaled the branch that anhydrates with thieving paper.
6) add 95% alcohol number droplet, and shake gently and decolour, 30 seconds after washings are inhaled the branch that anhydrates.
7) after luxuriant red colouring liquid (rare) (or husky yellow) dyed for 10 seconds, the tap water flushing.Drying, microscopy.
Painted result, gram positive reaction thalline all is purple, and the negative reaction thalline all takes on a red color.
4 step of gram's staining Color is fine, but the operation more complicated, modern laboratory work is busy, and every technological method is had the requirement of making every effort to easy, so that finish more task in the limited time.
Summary of the invention
Purpose of the present invention be exactly provide in order to overcome the defective that above-mentioned prior art exists a kind of easy and simple to handle, method reliable, the gram stain two-step method automatic staining instrument of stable performance.
Purpose of the present invention can be achieved through the following technical solutions:
A kind of gram stain two-step method automatic staining instrument, it is characterized in that, this dyeing instrument is by color pond, service sink, the dry pond of current of warm air, mechanical manipulator, track and display screen are formed, described color pond has two, first color pond, second color pond, the dry pond of service sink and current of warm air is arranged in order the front portion that is arranged on dyeing instrument, described mechanical manipulator is located at the top of dyeing instrument, can place in the front end of this mechanical manipulator and wait to dye sheet or Quality Control sheet, the rear end of mechanical manipulator is connected with described track, described display screen is located at a side at dyeing instrument top, getting material to be checked is evenly coated in and waits to dye on sheet or the Quality Control sheet, to wait to dye sheet or the Quality Control sheet places mechanical manipulator, mechanical manipulator places first color pond to carry out the first step dyeing front end by lifting moving, again by mechanical manipulator moving horizontally in orbit, mechanical manipulator is placed second color pond successively, service sink, in the dry pond of current of warm air, through the automatic staining second time, clean and drying after, treat again and dye sheet or the Quality Control sheet carries out microscopy.
Dyestuff in described first color pond is the Viola crystallina aqueous solution of 0.5-1.0wt%.
Dyestuff in described second color pond is the ethanolic soln of basic fuchsin, iodine and potassiumiodide.
The concentration of described basic fuchsin is 0.2-0.4wt%, and the concentration of described iodine is 0.1-0.2wt%, and the concentration of described potassiumiodide is 0.4-0.6wt%.
Temperature is 50-60 ℃ in the dry pond of described current of warm air.
Described mechanical manipulator can level reach vertical moving, and this mechanical manipulator front end is placed the dry pond of color pond, service sink and current of warm air successively.
The front portion of described mechanical manipulator can be placed the 6-24 sheet and be waited to dye sheet or Quality Control sheet.
Described material to be checked comprises the bacterial cultures that contains gram-positive microorganism and Gram-negative bacteria or secretory product etc.
This automatic staining instrument also can be applicable in the antiacid and blood cell staining of two step method.
Compared with prior art, the present invention has the following advantages:
(1) use production technique of the present invention and operating process simple, water quality do not had particular requirement, produce and checkout procedure in no poisonous, objectionable impurities input and generation;
(2) the present invention can provide quicker, easy standardized experimental result, and microscopy behind the automatic staining can be seen in smear central authorities and to dye the red gram-negative bacteria and the gram positive organism of intense violet color;
(3) the present invention integrate fixing, dyeing, clean, dry; Per hour multipotency dyes 150, short run in enormous quantities dye sheet all can, efficient is higher, easy and simple to handle, friendly interface, result are easy to observe and judge;
(4) sample presentation is easy, and promptly to promptly doing, continuity is strong for sample, and the intellectualized operation of employing is sample storage automatically;
(5) the present invention helps biology or medical practice, helps the commercialization transportation, and safe and reliable, and very big potential market is arranged, and can produce tangible society and economic benefit.
Description of drawings
Fig. 1 is a structural representation of the present invention.
1 is that first color pond, 2 is that second color pond, 3 is that service sink, 4 is that the dry pond of current of warm air, 5 is that mechanical manipulator, 6 is that track, 7 is a display screen among the figure.
Embodiment
The present invention is described in detail below in conjunction with the drawings and specific embodiments.
A kind of gram stain two-step method automatic staining instrument, its structure as shown in Figure 1.This dyeing instrument is made up of first color pond 1, second color pond 2, service sink 3, the dry pond 4 of current of warm air, mechanical manipulator 5, track 6 and display screen 7, and the dry pond 4 of first color pond 1, second color pond 2, service sink 3 and current of warm air is arranged in order the front portion that is arranged on dyeing instrument.Dyestuff in first color pond 1 is the Viola crystallina aqueous solution of 0.5wt%, dyestuff in second color pond 2 is the ethanolic soln of basic fuchsin, iodine and potassiumiodide, wherein the concentration of basic fuchsin is 0.3wt%, the concentration of iodine is 0.1wt%, and the concentration of potassiumiodide is 0.6wt%, overflows draining by service sink 3, when dyeing, can not cause the excessive and environmental pollution of dye liquor, temperature is 50 ℃ in the dry pond 4 of current of warm air, fixes and seasoning without flame.Mechanical manipulator 5 is located at the top of dyeing instrument, place 6 Quality Control sheets in the front end of this mechanical manipulator 5, the rear end of mechanical manipulator 5 is connected with track 6, and mechanical manipulator 5 can level and vertically moved, and places the dry pond 4 of color pond, service sink 3 and current of warm air to carry out dying operation successively the front end of this mechanical manipulator 5.Display screen 7 is located at a side at dyeing instrument top, can show the operating parameters of this dyeing instrument, comprises voltage 100/260V, frequency 50/60HZ, power 250W etc.
Get the Quality Control sheet of 6 cleanings, get the even smear of material bacteria culture to be checked, go up machine then, the Quality Control sheet is placed mechanical manipulator 5, mechanical manipulator 5 places first color pond 1 to carry out the first step dyeing front end by lifting moving, again by mechanical manipulator 5 moving horizontally on track 6, mechanical manipulator 5 is placed second color pond 2 successively, service sink 3, in the dry pond 4 of current of warm air, through the automatic staining second time, after cleaning and the drying, again the Quality Control sheet is carried out microscopy, see the gram positive organism of dying ruddy gram-negative bacteria and dying intense violet color in smear central authorities, with distinct contrast.
A kind of gram stain two-step method automatic staining instrument, this dyeing instrument is made up of first color pond, second color pond, service sink, the dry pond of current of warm air, mechanical manipulator, track and display screen, and the dry pond of first color pond, second color pond, service sink and current of warm air is arranged in order the front portion that is arranged on dyeing instrument.Dyestuff in first color pond is the Viola crystallina aqueous solution of 1.0wt%, dyestuff in second color pond is the ethanolic soln of basic fuchsin, iodine and potassiumiodide, wherein the concentration of basic fuchsin is 0.2wt%, the concentration of iodine is 0.1wt%, and the concentration of potassiumiodide is 0.4wt%, overflows draining by service sink, when dyeing, can not cause the excessive and environmental pollution of dye liquor, temperature is 50 ℃ in the dry pond of current of warm air, fixes and seasoning without flame.Mechanical manipulator is located at the top of dyeing instrument, place 12 sheets to be dyed in the front end of this mechanical manipulator, the rear end of mechanical manipulator is connected with described track, and mechanical manipulator can level reach vertical moving, and places the dry pond of color pond, service sink and current of warm air to carry out dying operation successively this mechanical manipulator front end.Display screen is located at a side at dyeing instrument top, can show the operating parameters of this dyeing instrument, comprises voltage 100/260V, frequency 50/60HZ, power 250W etc.
Get the sheet to be dyed of 12 cleanings, get material phlegm to be checked and the even smear of fester, go up machine then, the Quality Control sheet is placed mechanical manipulator, mechanical manipulator places first color pond to carry out the first step dyeing front end by lifting moving, again by mechanical manipulator moving horizontally in orbit, mechanical manipulator is placed second color pond, service sink, the dry pond of current of warm air successively, after second time automatic staining, cleaning and drying, again the Quality Control sheet is carried out microscopy, see the gram positive organism of dying ruddy gram-negative bacteria and dying intense violet color in smear central authorities, with distinct contrast.
A kind of gram stain two-step method automatic staining instrument, this dyeing instrument is made up of first color pond, second color pond, service sink, the dry pond of current of warm air, mechanical manipulator, track and display screen, and the dry pond of first color pond, second color pond, service sink and current of warm air is arranged in order the front portion that is arranged on dyeing instrument.Dyestuff in first color pond is the Viola crystallina aqueous solution of 0.5wt%, dyestuff in second color pond is the ethanolic soln of basic fuchsin, iodine and potassiumiodide, wherein the concentration of basic fuchsin is 0.4wt%, the concentration of iodine is 0.2wt%, and the concentration of potassiumiodide is 0.6wt%, overflows draining by service sink, when dyeing, can not cause the excessive and environmental pollution of dye liquor, temperature is 60 ℃ in the dry pond of current of warm air, fixes and seasoning without flame.Mechanical manipulator is located at the top of dyeing instrument, place 24 sheets to be dyed in the front end of this mechanical manipulator, the rear end of mechanical manipulator is connected with described track, and mechanical manipulator can level reach vertical moving, and places the dry pond of color pond, service sink and current of warm air to carry out dying operation successively this mechanical manipulator front end.Display screen is located at a side at dyeing instrument top, can show the operating parameters of this dyeing instrument, comprises voltage 100/260V, frequency 50/60HZ, power 250W etc.
Get the sheet to be dyed of 24 cleanings, get material urine to be checked, vaginal secretion and ight soil, even smear, go up machine then, the Quality Control sheet is placed mechanical manipulator, mechanical manipulator places first color pond to carry out the first step dyeing front end by lifting moving, again by mechanical manipulator moving horizontally in orbit, mechanical manipulator is placed second color pond successively, service sink, in the dry pond of current of warm air, through the automatic staining second time, after cleaning and the drying, again the Quality Control sheet is carried out microscopy, see the gram positive organism of dying ruddy gram-negative bacteria and dying intense violet color in smear central authorities, with distinct contrast.
Claims (9)
1. gram stain two-step method automatic staining instrument, it is characterized in that, this dyeing instrument is by color pond, service sink, the dry pond of current of warm air, mechanical manipulator, track and display screen are formed, described color pond has two, first color pond, second color pond, the dry pond of service sink and current of warm air is arranged in order the front portion that is arranged on dyeing instrument, described mechanical manipulator is located at the top of dyeing instrument, can place in the front end of this mechanical manipulator and wait to dye sheet or Quality Control sheet, the rear end of mechanical manipulator is connected with described track, described display screen is located at a side at dyeing instrument top, getting material to be checked is evenly coated in and waits to dye on sheet or the Quality Control sheet, to wait to dye sheet or the Quality Control sheet places mechanical manipulator, mechanical manipulator places first color pond to carry out the first step dyeing front end by lifting moving, again by mechanical manipulator moving horizontally in orbit, mechanical manipulator is placed second color pond successively, service sink, in the dry pond of current of warm air, through the automatic staining second time, clean and drying after, treat again and dye sheet or the Quality Control sheet carries out microscopy.
2. a kind of gram stain two-step method automatic staining instrument according to claim 1 is characterized in that, the dyestuff in described first color pond is the Viola crystallina aqueous solution of 0.5-1.0wt%.
3. a kind of gram stain two-step method automatic staining instrument according to claim 1 is characterized in that, the dyestuff in described second color pond is the ethanolic soln of basic fuchsin, iodine and potassiumiodide.
4. a kind of gram stain two-step method automatic staining instrument according to claim 3 is characterized in that the concentration of described basic fuchsin is 0.2-0.4wt%, and the concentration of described iodine is 0.1-0.2wt%, and the concentration of described potassiumiodide is 0.4-0.6wt%.
5. a kind of gram stain two-step method automatic staining instrument according to claim 1 is characterized in that, temperature is 50-60 ℃ in the dry pond of described current of warm air.
6. a kind of gram stain two-step method automatic staining instrument according to claim 1 is characterized in that, described mechanical manipulator can level reach vertical moving, and this mechanical manipulator front end is placed the dry pond of color pond, service sink and current of warm air successively.
7. a kind of gram stain two-step method automatic staining instrument according to claim 1 is characterized in that the front portion of described mechanical manipulator can be placed the 6-24 sheet and be waited to dye sheet or Quality Control sheet.
8. a kind of gram stain two-step method automatic staining instrument according to claim 1 is characterized in that, described material to be checked comprises the bacterial cultures that contains gram-positive microorganism and Gram-negative bacteria or secretory product etc.
9. a kind of gram stain two-step method automatic staining instrument according to claim 1 is characterized in that this automatic staining instrument also can be applicable in the antiacid and blood cell staining of two step method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101484403A CN102220236A (en) | 2010-04-15 | 2010-04-15 | Automatic staining instrument with Gram staining two-step method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101484403A CN102220236A (en) | 2010-04-15 | 2010-04-15 | Automatic staining instrument with Gram staining two-step method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102220236A true CN102220236A (en) | 2011-10-19 |
Family
ID=44776945
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101484403A Pending CN102220236A (en) | 2010-04-15 | 2010-04-15 | Automatic staining instrument with Gram staining two-step method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102220236A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103674660A (en) * | 2012-09-12 | 2014-03-26 | 无锡飞伊生物科技有限公司 | Gram staining two-step method with one-time washing |
| CN104560701A (en) * | 2015-01-19 | 2015-04-29 | 枣庄学院 | Automatic bacterial spore stainer |
| CN106769351A (en) * | 2015-11-23 | 2017-05-31 | 内蒙古蒙牛乳业（集团）股份有限公司 | Microbial staining frame |
| CN107478485A (en) * | 2017-06-30 | 2017-12-15 | 严铭 | Gram's staining instrument |
| WO2018145386A1 (en) * | 2017-02-08 | 2018-08-16 | 爱威科技股份有限公司 | Sample staining device |
| CN108663253A (en) * | 2018-08-14 | 2018-10-16 | 苏州丰泰医疗用品贸易有限公司 | One kind quickly carrying out Gram-stained method |
| CN108918238A (en) * | 2018-07-30 | 2018-11-30 | 洛阳师范学院 | It is a kind of using capillary as the Gram-staining process of carrier and device |
| CN111307560A (en) * | 2020-03-05 | 2020-06-19 | 同济大学 | Automatic urine cell staining device and control system |
| CN111337322A (en) * | 2014-04-28 | 2020-06-26 | 深圳迈瑞生物医疗电子股份有限公司 | Smear preparation device and smear preparation method |
-
2010
- 2010-04-15 CN CN2010101484403A patent/CN102220236A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103674660A (en) * | 2012-09-12 | 2014-03-26 | 无锡飞伊生物科技有限公司 | Gram staining two-step method with one-time washing |
| CN111337322A (en) * | 2014-04-28 | 2020-06-26 | 深圳迈瑞生物医疗电子股份有限公司 | Smear preparation device and smear preparation method |
| CN104560701A (en) * | 2015-01-19 | 2015-04-29 | 枣庄学院 | Automatic bacterial spore stainer |
| CN106769351A (en) * | 2015-11-23 | 2017-05-31 | 内蒙古蒙牛乳业（集团）股份有限公司 | Microbial staining frame |
| CN106769351B (en) * | 2015-11-23 | 2023-10-10 | 内蒙古蒙牛乳业（集团）股份有限公司 | Microorganism staining rack |
| WO2018145386A1 (en) * | 2017-02-08 | 2018-08-16 | 爱威科技股份有限公司 | Sample staining device |
| RU2737469C1 (en) * | 2017-02-08 | 2020-11-30 | АВЕ САЙЕНС энд ТЕКНОЛОДЖИ КО., ЛТД. | Device for coloring samples |
| US11513040B2 (en) | 2017-02-08 | 2022-11-29 | Ave Science & Technology Co., Ltd. | Sample staining device |
| CN107478485A (en) * | 2017-06-30 | 2017-12-15 | 严铭 | Gram's staining instrument |
| CN108918238A (en) * | 2018-07-30 | 2018-11-30 | 洛阳师范学院 | It is a kind of using capillary as the Gram-staining process of carrier and device |
| CN108663253A (en) * | 2018-08-14 | 2018-10-16 | 苏州丰泰医疗用品贸易有限公司 | One kind quickly carrying out Gram-stained method |
| CN111307560A (en) * | 2020-03-05 | 2020-06-19 | 同济大学 | Automatic urine cell staining device and control system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102220236A (en) | Automatic staining instrument with Gram staining two-step method | |
| CN102876081B (en) | Hematoxylin staining solution and papanicolaou staining kit containing same | |
| McKINNEY | Staining bacterial polysaccharides | |
| CN107490511A (en) | A kind of haematoxylin dyeing liquid and HE colouring methods | |
| CN104777026A (en) | Liquid-dropping type automatic biological staining instrument | |
| CN101251449A (en) | Method for staining medical tissue slice | |
| CN104777027A (en) | Multifunctional full-automatic gram staining instrument | |
| CN101126689B (en) | Gram stain two-step method | |
| CN201689002U (en) | Automatic staining device adopting two-step Gram stain | |
| CN108731994A (en) | A kind of production method of climbing groundsel root tip chromosomes sample slice | |
| CN107219111A (en) | A kind of chromosome flaking method of distant hybridization beet | |
| EP0286255B1 (en) | Improved stain for acid-fast bacilli | |
| CN112129610B (en) | Bacterial capsule staining method and application thereof | |
| CN108535077A (en) | A kind of pap staining liquid and application process | |
| CN101923019B (en) | Method for restoring color of faded pathological HE section | |
| Chance | Crystal violet as a nuclear stain for Gaffkya tetragena and other bacteria | |
| CN104819886B (en) | A kind of bacterial capsule rapid dyeing reagent and its colouring method | |
| CN107558261A (en) | A kind of woolen dyed special granatum dyestuff | |
| Yegian et al. | Factors affecting the beading of the tubercle bacillus stained by the Ziehl-Neelsen technique | |
| CN102879251A (en) | Dehydrating and dyeing dual-function machine and control method thereof | |
| CN202903569U (en) | Dehydrating and dyeing difunctional machine | |
| CN103674660A (en) | Gram staining two-step method with one-time washing | |
| CN103674661A (en) | Washing-free Gram staining method | |
| CN207567232U (en) | One kind determines whether thalline detection device | |
| LU101831B1 (en) | A Method for Staining Adipocytes and Application Thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20111019 |