CN103412116B - Full-automatic tubercle bacillus coloring system - Google Patents
Full-automatic tubercle bacillus coloring system Download PDFInfo
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- CN103412116B CN103412116B CN201310315349.XA CN201310315349A CN103412116B CN 103412116 B CN103412116 B CN 103412116B CN 201310315349 A CN201310315349 A CN 201310315349A CN 103412116 B CN103412116 B CN 103412116B
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Abstract
The invention provides a kind of full-automatic tubercle bacillus coloring system, comprise dyeing centrifugal mechanism, control device and flow path mechanism, control device controls dyeing centrifugal mechanism according to user instruction and flow path mechanism performs dyeing flow process; Flow path mechanism comprises heating system, shower nozzle, and in flow path mechanism, heating system is connected to the front end of shower nozzle, and heating system is used for first heating reagent before shower nozzle ejection at reagent.The present invention has that closed loop constant-temperature heating system, colouring method are optional, non-contact type sprays trigging control and surplus detection system, the time unification of the processes such as dyeing, cleaning can be ensured, solution use amount is unified, obtains the beneficial effect such as robotization, unitarity, fast saving.
Description
Technical field
The present invention relates to biological stain system, particularly, relate to full-automatic tubercle bacillus coloring system.
Background technology
WHO report shows: at present, and nearly 2,000,000,000 populations in the whole world are by the infection of tulase.Have 2,000 ten thousand people to suffer from tuberculosis, annual neopathy people reaches 800 ~ 1,000 ten thousand people.Annual about 3,000,000 people die from tuberculosis, and tuberculosis becomes the number one killer of infectious disease.If do not taked effective control measure immediately, within 10 years from now on, will have 300,000,000 Healthy Peoples by tubercle bacillus affection, there is tuberculosis in 9,000 ten thousand people, 3,000 ten thousand people die from tuberculosis.
Much's bacillus is referred to as tubercle bacillus.Much's bacillus size 1 ~ 4 × 0.3 ~ 0.6 μm.Thalline is elongated and slightly curved, and two ends are slightly blunt.The acid-fast stain positive is its key property.M. tuberculosis growth is slow, at least needs 2-4 week just to occur visible colonies.
Mycobacterium cell wall is rich in lipid, accounts for 60% of cell membrane, and main composition is the acid of branch bar and acidifying trehalose.The former is the material base of antiacid reaction; The latter comprises the two mycolic acid of marine alga and the sweet acid of sulphur, and the effect having mediation Granuloma formation respectively and protect bacterium to survive in phagocyte, is considered to the virulence factor of Much's bacillus.Still containing lipopolysaccharides in cell membrane, wherein LAM has immunogenicity widely, and the Much's bacillus in growth can produce LAM in a large number, is to apply a more quasi-antigen substance in serodiagnosis.
Mycobacterium cell wall is rich in lipid, generally not easy coloring, heats or extends dyeing time, it can be made painted, and not easily by hydrochloride alcohol is decoloured, make thalline dye redness with intensive (5% carbolic acid azaleine solution).Non-acid fast bacteria is easily hydrochloride alcohol decolouring, then redyes with methylene blue and make in blue.
China belongs to one of 22 high popular countries of tuberculosis in the world, there are more than 500,000,000 populations in the whole nation by tubercle bacillus affection, active tuberculosis patient reaches more than 600 ten thousand, wherein infectiousness pulmonary tuberculosis patient reaches more than 200 ten thousand, annual newly-increased 1,130,000 new tuberculosis patients, also have a large amount of extrapulmonary tuberculosis people to exist, every year because tuberculosis died reaches 250,000, tuberculosis remains the serious public health problem of a harm people ' s health in China.
Present stage, the process of tuberculosis sample, is also in the manual stage.The basic procedure of manual acid-fast stain is:
(1) be coated with to wipe one's face and cover with filter paper, then on filter paper, drip carbolfuchsin dye liquor, filter paper is soaked by dye liquor completely, hold slide and heat on little fire and then leave flame a moment, now can see steam that slide emerges.Heat after steam disappears, 3 ~ 4 times so repeatedly, about 5min, adds dye liquor at any time and does not make the scraps of paper dry up when heating again.
(2), after slide cooling, choose filter paper with oese and throw away in dirt basin, running water slide.
(3) 3% hydrochloride alcohol decolouring is dripped, to smearing even position do not have redness to take off substantially again, washing.
(4) Lv Shi methylene blue dye liquor 30sec is dripped, washing.
(5) blot, prepare microscopy.
Manual operations inefficiency, result hand-manipulated varies with each individual, and be difficult to the accuracy and the promptness that ensure clinical sample Micro biological Tests, and manual workload is large, is unfavorable for the risk reducing operating personnel.Therefore be necessary to develop a kind of automation equipment, thus greatly can alleviate the workload of operating personnel, and reduce the risk of operating personnel.
Summary of the invention
For defect of the prior art, the object of this invention is to provide a kind of full-automatic tubercle bacillus coloring system.
According to an aspect of the present invention, provide a kind of full-automatic tubercle bacillus coloring system, comprise dyeing centrifugal mechanism, control device and flow path mechanism, control device controls dyeing centrifugal mechanism according to user instruction and flow path mechanism performs dyeing flow process.
Preferably, flow path mechanism comprises heating system, shower nozzle, and in flow path mechanism, heating system is connected to the front end of shower nozzle, and heating system is used for first heating reagent before shower nozzle ejection at reagent.
Preferably, described heating system is constant-temperature heating system, and constant-temperature heating system comprises: temperature sensor, well heater, MCU, MCU connect temperature sensor, well heater, wherein:
Temperature sensor is used for the temperature value of the reagent detected to feed back to MCU, whether MCU controls control heater according to temperature value and works, be specially: when this temperature value is lower than temperature threshold, MCU control heater heats reagent, until the temperature value of reagent arrives temperature threshold, MCU control heater stops heating.
Preferably, control device stores multiple dyeing flow process, described user instruction is used to indicate the identification information of dyeing flow process, control device determines a kind of pending dyeing flow process according to identification information from described multiple dyeing flow process, and then control dyeing centrifugal mechanism and flow path mechanism perform this pending dyeing flow process.
Preferably, described dyeing flow process comprise following any one or appoint multiple tuberculosis dyeing flow:
Tuberculosis dyeing flow A:
-basic fuchsin dye liquor is heated to 70 degrees Celsius;
-with the basic fuchsin dye liquors of 70 degrees Celsius, sample is dyeed 1 minute;
-use washed with de-ionized water 1 minute;
-with destainer, sample is taken off to colourless, wherein, destainer is made up of the 10 times of dilutions of 5% vinyl alcohol solution;
-use washed with de-ionized water 30 seconds;
-redye with redying liquid, wherein, redying liquid is methylene blue;
-use washed with de-ionized water;
Tuberculosis dyeing flow B:
-with auramine dyeing liquor, dyeing is carried out 1 minute to sample;
-use washed with de-ionized water;
-with destainer, sample is taken off to colourless;
-use washed with de-ionized water 30 seconds;
-redye with redying liquid;
-use washed with de-ionized water;
Tuberculosis dyeing flow C:
-basic fuchsin dye liquor is heated to 70 degrees Celsius;
-with the basic fuchsin dye liquor of 70 degrees Celsius, sample is dyeed;
-use washed with de-ionized water;
-with destainer, sample is decoloured, wherein, destainer is made up of the 10 times of dilutions of 5% vinyl alcohol solution;
-use washed with de-ionized water;
-redye with redying liquid, wherein, redying liquid is methylene blue;
-use washed with de-ionized water.
Preferably, dyeing centrifugal mechanism comprises several Magnetic Sensors, and wherein, Magnetic Sensor is arranged on the dyeing cavity outer wall of dyeing centrifugal mechanism, Magnetic Sensor connection control device, control device controls flow path mechanism reagent injected according to the signal being received from Magnetic Sensor.
Preferably, 2 Magnetic Sensors are had at least to be separately positioned on dyeing cavity outer wall, the center of circle of carrying glass dish of these 2 Magnetic Sensors and dyeing centrifugal mechanism is positioned at same plane, and the position of placing Magnetic Sensor is fixing, and namely the position of the nozzle of flow path mechanism is also fixing relatively.
Preferably, also comprise specimen holder, wherein, specimen holder is arranged in dyeing cavity, No. 1 sample position of specimen holder and No. 2 sample positions is provided with two different permanent magnets.
Preferably, when the permanent magnet on sample position runs to the sensed position of Magnetic Sensor, Magnetic Sensor by signal transmission to control device, the signal according to Magnetic Sensor followed by control device, determine the position of the nozzle of the relative flow path mechanism in sample position, and per sample frame dyeing time rotating speed, calculate after how long, this sample position is through nozzle thus spray.
Preferably, flow path mechanism comprises weight sensor, wherein, weight sensor is positioned at the below of the reagent bottle of flow path mechanism, for weighing reagent, whether control device according to being received from the input reagent of weight sensor lower than lowest threshold, if detect, reagent is lower than lowest threshold, then control dyeing centrifugal mechanism and flow path mechanism out of service.
Compared with prior art, the present invention has following beneficial effect:
(1) solve traditional many drawbacks brought because of manual operations, a large amount of false positive that such as manual test causes and false-negative experimental result, the skill level of operating personnel is different, and the effect dyed has a lot of difference etc.; Relative to manual operations, cost of the present invention doubly improves the efficiency of acid-fast stain, greatly saves the time of operating personnel; Pass through robotization, standardized dyeing flow, efficiently avoid the false positive of traditional-handwork dyeing or false-negative fruiting quantities, full-automatic tuberculosis coloring system effectively solves the error of individual differences agency, the temperature of dye liquor can be controlled consistently, can automatically according to the fine setting of sample thickness setting dyeing time, all operations carries out under homogeneous condition, therefore greatly reduces experimental error.
(2) save the unnecessary waste of the test reagent caused because of manual experimental implementation, bring more economic benefit to laboratory; Save reagent cost, increase customer satisfaction.The manual tuberculosis dyeing course of relatively traditional people, experiment reagent use amount is difficult to control, the present invention is not when specimen holder carries full, the consumption that sample position can greatly reduce experiment reagent is detected by Magnetic Sensor, improve conventional efficient, adopting the reagent of full-automatic coloring system to save can reach 30-50%.
(3) reduce the discharge of laboratory harmful reagent, accomplish the pollution that effectively prevent the improper environment brought of microbiological manipulations, the impact on experimenter that the volatilization reducing lime acid azaleine and the sample do not processed produce; Stop laboratory cross pollution--traditional tuberculosis dyeing is because reagent contamination causes experiment generally to be carried out on limit, pond, although microorganism all needs through deactivation, but because manually-operated uncertainty causes pollution that unnecessary personnel may be caused to infect the latter cause laboratory pollution, full automatic coloring system carries out in an airtight environment, thus the cross pollution in the laboratory of stopping, thus reach better biological safety.
(4) lime acid azaleine needs at 70 degree to be that competence exertion goes out best Color, the invention solves to the problem of lime acid azaleine solution heated at constant temperature.
(5) the present invention supports multiple Staining Protocol simultaneously, comprises Z-H decoration method, fluorescence colour, the methods such as Kinyoun decoration method.
Accompanying drawing explanation
By reading the detailed description done non-limiting example with reference to the following drawings, other features, objects and advantages of the present invention will become more obvious:
Fig. 1 is one-piece construction schematic diagram of the present invention;
Fig. 2 is that tuberculosis dyes three kinds of schematic flow sheets;
Fig. 3 is the structural representation carrying glass dish;
Fig. 4, Fig. 5 are the structural representation of constant-temperature heating system;
Fig. 6 is the principle schematic that surplus detects;
Fig. 7 is planimetric map and the sectional view of specimen holder.
In figure:
1 is dyeing cavity;
2 is reagent exit;
3 is alcohol entrance;
4 is well heater;
5 is reagent inlet;
6 is temperature sensor;
7 is heater line;
8 is pipeline;
9 is reagent bottle;
10 is reagent tray;
11 is LOAD CELLS;
12 is weighing circuit plate;
13 is No. 1 position permanent magnet;
14 is No. 1 sample position;
15 is No. 7 sample positions;
16 is No. 2 position permanent magnets;
17 is microslide;
18 is sample rack.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.Following examples will contribute to those skilled in the art and understand the present invention further, but not limit the present invention in any form.It should be pointed out that to those skilled in the art, without departing from the inventive concept of the premise, some distortion and improvement can also be made.These all belong to protection scope of the present invention.
Full-automatic tubercle bacillus coloring system provided by the invention, comprises dyeing centrifugal mechanism, control device and flow path mechanism.Control device controls dyeing centrifugal mechanism according to user instruction and flow path mechanism performs dyeing flow process, particularly, control device stores multiple dyeing flow process, described user instruction is used to indicate the identification information of dyeing flow process, control device determines a kind of pending dyeing flow process according to identification information from described multiple dyeing flow process, then control dyeing centrifugal mechanism and flow path mechanism perform this pending dyeing flow process, achieve a multiple dyeing flow of instrument.Wherein, described dyeing flow process comprise following any one or appoint multiple tuberculosis dyeing flow:
Tuberculosis dyeing flow A:
-basic fuchsin dye liquor is heated to 70 degrees Celsius;
-with the basic fuchsin dye liquors of 70 degrees Celsius, sample is dyeed 1 minute;
-use washed with de-ionized water 1 minute;
-with destainer, sample is taken off to colourless, wherein, destainer is made up of the 10 times of dilutions of 5% vinyl alcohol solution;
-use washed with de-ionized water 30 seconds;
-redye with redying liquid, wherein, redying liquid is methylene blue;
-use washed with de-ionized water;
Tuberculosis dyeing flow B:
-with auramine dyeing liquor, dyeing is carried out 1 minute to sample;
-use washed with de-ionized water;
-with destainer, sample is taken off to colourless;
-use washed with de-ionized water 30 seconds;
-redye with redying liquid;
-use washed with de-ionized water;
Tuberculosis dyeing flow C:
-basic fuchsin dye liquor is heated to 70 degrees Celsius;
-with the basic fuchsin dye liquor of 70 degrees Celsius, sample is dyeed;
-use washed with de-ionized water;
-with destainer, sample is decoloured, wherein, destainer is made up of the 10 times of dilutions of 5% vinyl alcohol solution;
-use washed with de-ionized water;
-redye with redying liquid, wherein, redying liquid is methylene blue;
-use washed with de-ionized water.
Further, flow path mechanism comprises heating system, shower nozzle, and in flow path mechanism, heating system is connected to the front end of shower nozzle, and heating system is used for first heating reagent before shower nozzle ejection at reagent.Inventor finds that lime acid azaleine only needs 30s just can reach the effect originally heating 5min on spirit lamp when being heated to 70 degree, so at solution before shower nozzle ejection, need being heated to solution constant temperature 70 degree and keeping constant temperature.
Particularly, described heating system is constant-temperature heating system, constant-temperature heating system comprises: liquid outlet, fixed interface, cleaning alcohol entrance, temperature sensor, well heater, reagent inlet, MCU, relay, wherein: temperature sensor is used for the temperature value of the reagent detected to feed back to MCU, whether MCU controls control heater according to temperature value and works, be specially: when this temperature value is lower than temperature threshold, MCU control heater heats reagent, until the temperature value of reagent arrives temperature threshold, MCU control heater stops heating.In a preference, the temperature value that temperature sensor detects feeds back to MCU, and MCU can according to the break-make of temperature value pilot relay, and whether the break-make control heater of relay works.When temperature sensor detects temperature lower than 70 degree, MCU can open relay, and well heater is heated, until when temperature value is 70, MCU meeting closed relay, makes well heater power-off.After stable, MCU can be constant to be energized power-off to well heater by certain frequency, make its maintain 70 degree invariable.
Further, dyeing centrifugal mechanism comprises several Magnetic Sensors, and wherein, Magnetic Sensor is arranged on the dyeing cavity outer wall of dyeing centrifugal mechanism, Magnetic Sensor connection control device, control device controls flow path mechanism reagent injected according to the signal being received from Magnetic Sensor.Have at least 2 Magnetic Sensors to be separately positioned on dyeing cavity outer wall, the center of circle of carrying glass dish of these 2 Magnetic Sensors and dyeing centrifugal mechanism is positioned at same plane.The present invention also comprises specimen holder, and wherein, specimen holder is arranged in dyeing cavity, and several sample positions of specimen holder are provided with permanent magnet, carries glass dish and adopts discrete design effectively to have accomplished to prevent cross pollution.
Like this, the present invention can realize touchless injection trigging control.Particularly, (carry glass dish schematic diagram when full sample do not filled by whole year glass dish and see Fig. 4), if reagent continues to spray with the amount of dishful sample, the waste of reagent will certainly be caused, so accurately sample will be located, when the position being loaded with sample arrives before nozzle, accurate reagent injected, when arriving in the position of sky, accurately stop spraying.Because sample is all be placed in whole dyeing cavity, and operationally, the gasoloid of reagent can be full of in whole dyeing cavity, so touchless effect mode can only be adopted, dyeing cavity outer wall settles high-precision Magnetic Sensor, as shown in Figure 4, places permanent magnet in No. 1 sample position and No. 6 sample positions place, whenever these two positions are through Magnetic Sensor, sprayed accurately with the position realized needing by the accurate position of the accurate judgement sample of Magnetic Sensor energy.
Such as, when No. 1 sample position (permanent magnet) runs to the position of Magnetic Sensor, Magnetic Sensor has signal transmission to control device, and control device can follow the signal according to Magnetic Sensor, knows the position of No. 1 sample position relative nozzle.Again because dyeing time rotating speed be that constant 20rpm, MCU can calculate after how long, that sample is through nozzle.By that analogy, the sample position that No. 2 sample positions are later also can accurately be located.
More further, flow path mechanism comprises weight sensor, wherein, weight sensor is positioned at the below of the reagent bottle of flow path mechanism, for weighing reagent, whether control device according to being received from the input reagent of weight sensor lower than lowest threshold, if detect, reagent is lower than lowest threshold, then control dyeing centrifugal mechanism and flow path mechanism out of service.
If reagent exhausts, instrument continues to run, and can cause many bad consequences, comprise: sample is discarded owing to not dyeing normally, need Resurvey even to cultivate, instrument zero load can cause the damage etc. of the bad or even vitals of the work of instrument component.The present invention has surplus detection system, as shown in Figure 6, it places weight sensor below reagent bottle, in a preference, when reagent is less than 50g, weight sensor can send a signal to the microprocessor as control device, and namely microprocessor can report to the police reagent deficiency, remind operator to change filling reagent, avoid the generation of unfavorable condition.
In a preferred embodiment of the present invention, first the micro-biological samples be fixed on microslide is placed in the centrifugal dyeing cabin of full-automatic tubercle bacillus coloring system, by micro fluidic control technology, corresponding dyestuff and reagent are injected in the smear field of microorganism by the angle designed according to tuberculosis dyeing order successively, again through wash-out, redye supervisor, in process, dyeing and decolouring all complete rapidly in the system, without the need to manual intervention, microslide is placed in sample tray, whole dyeing, centrifugal, decolouring and process of redying all completed within 5 minutes, program can according to operator need select different dyeing flow and dyeing thickness.
Further, the constant-temperature heating system in the present invention is more accurate, and target temperature is adjustable, the reagent of target temperature, after shower nozzle atomization, more desirable in conjunction with effect.Better optimization is carried out for the shower nozzle tested and flow path system, has made reagent more save flow.Further, system provided by the invention compensate for well because the many inconvenient characteristics brought hand-manipulated and the disadvantage easily polluted, and has and is easy to operation, save reagent cost, avoid the improper feature polluted of microbiological manipulations.And automation process, strictly control dyeing, decolouring, the time of process such as to redye, the accuracy of the experiment improved greatly and saved the time of experimenter.Therefore the present invention can ensure the time unification of the processes such as dyeing, cleaning, and solution use amount is unified, embodies the feature such as robotization, unitarity, fast saving fully.
Above specific embodiments of the invention are described.It is to be appreciated that the present invention is not limited to above-mentioned particular implementation, those skilled in the art can make various distortion or amendment within the scope of the claims, and this does not affect flesh and blood of the present invention.
Claims (8)
1. a full-automatic tubercle bacillus coloring system, comprises dyeing centrifugal mechanism, control device and flow path mechanism, it is characterized in that, control device controls dyeing centrifugal mechanism according to user instruction and flow path mechanism performs dyeing flow process;
Control device stores multiple dyeing flow process, described user instruction is used to indicate the identification information of dyeing flow process, control device determines a kind of pending dyeing flow process according to identification information from described multiple dyeing flow process, and then control dyeing centrifugal mechanism and flow path mechanism perform this pending dyeing flow process;
Described dyeing flow process comprise following any one or appoint multiple tuberculosis dyeing flow:
Tuberculosis dyeing flow A:
-basic fuchsin dye liquor is heated to 70 degrees Celsius;
-with the basic fuchsin dye liquors of 70 degrees Celsius, sample is dyeed 1 minute;
-use washed with de-ionized water 1 minute;
-with destainer, sample is taken off to colourless, wherein, destainer is made up of the 10 times of dilutions of 5% vinyl alcohol solution;
-use washed with de-ionized water 30 seconds;
-redye with redying liquid, wherein, redying liquid is methylene blue;
-use washed with de-ionized water;
Tuberculosis dyeing flow B:
-with auramine dyeing liquor, dyeing is carried out 1 minute to sample;
-use washed with de-ionized water;
-with destainer, sample is taken off to colourless;
-use washed with de-ionized water 30 seconds;
-redye with redying liquid;
-use washed with de-ionized water;
Tuberculosis dyeing flow C:
-basic fuchsin dye liquor is heated to 70 degrees Celsius;
-with the basic fuchsin dye liquor of 70 degrees Celsius, sample is dyeed;
-use washed with de-ionized water;
-with destainer, sample is decoloured, wherein, destainer is made up of the 10 times of dilutions of 5% vinyl alcohol solution;
-use washed with de-ionized water;
-redye with redying liquid, wherein, redying liquid is methylene blue;
-use washed with de-ionized water.
2. full-automatic tubercle bacillus coloring system according to claim 1, it is characterized in that, flow path mechanism comprises heating system, shower nozzle, in flow path mechanism, heating system is connected to the front end of shower nozzle, and heating system is used for first heating reagent before shower nozzle ejection at reagent.
3. full-automatic tubercle bacillus coloring system according to claim 2, it is characterized in that, described heating system is constant-temperature heating system, and constant-temperature heating system comprises: temperature sensor, well heater, MCU, MCU connect temperature sensor, well heater, wherein:
Temperature sensor is used for the temperature value of the reagent detected to feed back to MCU, whether MCU controls control heater according to temperature value and works, be specially: when this temperature value is lower than temperature threshold, MCU control heater heats reagent, until the temperature value of reagent arrives temperature threshold, MCU control heater stops heating.
4. full-automatic tubercle bacillus coloring system according to claim 1, it is characterized in that, dyeing centrifugal mechanism comprises several Magnetic Sensors, wherein, Magnetic Sensor is arranged on the dyeing cavity outer wall of dyeing centrifugal mechanism, Magnetic Sensor connection control device, control device controls flow path mechanism reagent injected according to the signal being received from Magnetic Sensor.
5. full-automatic tubercle bacillus coloring system according to claim 4, it is characterized in that, 2 Magnetic Sensors are had at least to be separately positioned on dyeing cavity outer wall, the center of circle of carrying glass dish of these 2 Magnetic Sensors and dyeing centrifugal mechanism is positioned at same plane, and the position of placing Magnetic Sensor is fixing, namely the position of the nozzle of flow path mechanism is also fixing relatively.
6. full-automatic tubercle bacillus coloring system according to claim 4, is characterized in that, also comprise specimen holder, and wherein, specimen holder is arranged in dyeing cavity, No. 1 sample position of specimen holder and No. 2 sample positions is provided with two different permanent magnets.
7. the full-automatic tubercle bacillus coloring system according to claim 4 or 6, it is characterized in that, when the permanent magnet on sample position runs to the sensed position of Magnetic Sensor, Magnetic Sensor by signal transmission to control device, the signal according to Magnetic Sensor followed by control device, determines the position of the nozzle of the relative flow path mechanism in sample position, and rotating speed when frame dyes per sample, calculate after how long, this sample position is through nozzle thus spray.
8. full-automatic tubercle bacillus coloring system according to claim 1, it is characterized in that, flow path mechanism comprises weight sensor, wherein, weight sensor is positioned at the below of the reagent bottle of flow path mechanism, and for weighing reagent, control device is according to whether being received from the input reagent of weight sensor lower than lowest threshold, if detect, reagent is lower than lowest threshold, then control dyeing centrifugal mechanism and flow path mechanism out of service.
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| CN107246988A (en) * | 2017-06-06 | 2017-10-13 | 珠海贝索生物技术有限公司 | A kind of acid-resisting bacterium colouring method |
| CN109238812A (en) * | 2018-10-26 | 2019-01-18 | 天津源天晟科技发展有限公司 | A kind of automatic staining device and its control method |
| CN112362434B (en) * | 2020-09-15 | 2022-03-22 | 安徽九陆生物科技有限公司 | Full-automatic liquid-based thin-layer cell sheet-making dyeing machine control system |
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| CN1398980A (en) * | 2001-07-23 | 2003-02-26 | 张华� | Staing-solution for fast detecting tubercle bacillus and method thereof |
| WO2007136781A2 (en) * | 2006-05-18 | 2007-11-29 | Ericson Daniel G | Rapid detection of mycobacterium tuberculosis and antimicrobial drug resistance |
| CN101303608A (en) * | 2008-06-12 | 2008-11-12 | 黄竞 | Multiplex temperature detect controller |
| CN202024919U (en) * | 2011-04-16 | 2011-11-02 | 湖州师范学院 | Acid-fast stain device for mycobacterium tuberculosis |
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