CN106947799A - A kind of lactophenol cotton blue dyeing liquor and preparation method thereof - Google Patents
A kind of lactophenol cotton blue dyeing liquor and preparation method thereof Download PDFInfo
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- CN106947799A CN106947799A CN201710222262.6A CN201710222262A CN106947799A CN 106947799 A CN106947799 A CN 106947799A CN 201710222262 A CN201710222262 A CN 201710222262A CN 106947799 A CN106947799 A CN 106947799A
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- Prior art keywords
- parts
- dyeing liquor
- blue dyeing
- cotton blue
- lactophenol cotton
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2304/00—Chemical means of detecting microorganisms
- C12Q2304/10—DNA staining
- C12Q2304/18—Thionin-type dyes, e.g. Azure, Toluidine Blue
Abstract
The invention discloses a kind of lactophenol cotton blue dyeing liquor and preparation method thereof.Wherein, the lactophenol cotton blue dyeing liquor includes 10 15 parts of PVA, 20 40 parts of lactic acid, 20 40 parts of phenol and 0.05 0.1 parts of aniline blues.The preparation method of the lactophenol cotton blue dyeing liquor comprises the following steps：10 15 parts of PVA powder are added in the container for filling water, container is heated, meanwhile, stirred using bar magnet；Sequentially add 20 40 parts of lactic acid, 20 40 parts of phenol crystallizations, 0.05 0.1 parts of aniline blues；Persistently solution is heated and solution is stirred and evenly mixed using bar magnet, until temperature is up to 70 90 DEG C, stops heating, lactophenol cotton blue dyeing liquor is made.The present invention can persistence fungi humidity strip or slide culture well, the scope of application is wider, the holding time is more long.
Description
Technical field
The present invention relates to Coplin liquid field, especially a kind of lactophenol cotton blue dyeing liquor and preparation method thereof.
Background technology
Fungi belongs to eucaryote, and without plastid, nutritional mode is absorbs, no phagocytosis, and its usual cell membrane contains
Chitin and beta glucan.The mankind, the disease of animal can be caused, referred to as nosomycosis, can also cause disease, the mankind's allergy of plant
Property disease and mycotoxicosis.
In medical domain, in order to understand the structure of fungi, it usually needs the slide for being loaded with fungi is dyed.It is existing
And common mounting glue（Resinene）Method be only applicable to the mounting of dry plate, it is impossible to mounting is carried out to the humidity strip after dyeing, fitted
It is restricted with scope, and the retention time after dyeing is shorter.
The content of the invention
The present invention provides a kind of lactophenol cotton blue dyeing liquor and preparation method thereof, can well persistence fungi humidity strip or
Slide culture, the scope of application wider, holding time is more long.
According to the first aspect of the invention, the present invention provides a kind of lactophenol cotton blue dyeing liquor, including 10-15 parts of PVA,
20-40 parts of lactic acid, 20-40 parts of phenol and 0.05-0.1 parts of aniline blues.
It is preferred that, PVA, lactic acid, the optimum quality ratio of phenol and aniline blue are 12:30:30:0.075.
According to the second aspect of the invention, the present invention provides a kind of preparation method of lactophenol cotton blue dyeing liquor, including such as
Lower step：10-15 parts of PVA powder are added in the container for filling water, container is heated, meanwhile, stirred using bar magnet；
Sequentially add 20-40 parts of lactic acid, 20-40 parts of phenol crystallizations, 0.05-0.1 parts of aniline blues；Persistently solution is heated and made
Solution is stirred and evenly mixed with bar magnet, until temperature reaches 70-90 DEG C, stops heating, lactophenol cotton blue dyeing liquor is made.
It is preferred that, PVA, lactic acid, the optimum quality ratio of phenol and aniline blue are 12:30:30:0.075.
It is preferred that, held in container for deionized water.
It is preferred that, when being heated to 80 DEG C, stop heating.
It is preferred that, also comprise the following steps：The lactophenol cotton blue dyeing liquor of preparation is dispensed into drop bottle, bottle cap is covered tightly,
Room temperature preservation.
The beneficial effects of the invention are as follows：
1. mounting can be carried out to the humidity strip after dyeing and dry plate, the scope of application is wider.
2. can persistence fungi humidity strip or slide culture well, dried on flat surface at least 24 hours, these
Mountant is permanently present, and will not be dissolved in ether, dimethylbenzene or ethanol.Number can be clearly maintained through fixed fungi structure
Year, preservation effect is more preferable.
3. can be in microscopical low power, high power and oily Microscopic observation hypha,hyphae and spore structure, and original dye
Color method can only be observed under low power and high power.
4. integrating the function of dyeing and can preserve standby checking functions, common dye liquor only has dyeing function.
Embodiment
The present invention is described in further detail below by embodiment.
The present invention provides a kind of lactophenol cotton blue dyeing liquor, including 10-15 parts of PVA, 20-40 parts of lactic acid, 20-40 parts of phenol
With 0.05-0.1 parts of aniline blues.Wherein, PVA reagents are used as strengthening the adhesion between dye layer and glass reason, hermetic barrier dyeing
Layer is aoxidized with air contact.Phenol reagent is used as the affinity of fixed enhancing mycelia, spore and coloring agent, improves Color,
Separately there is disinfective action.Lactic acid reagent increases dissolubility with PH is made adjustments.Aniline blue reagent plays the work of dyeing as colouring agent
With.
The present invention also provides a kind of preparation method of lactophenol cotton blue dyeing liquor, comprises the following steps：By 10-15 parts of PVA
Powder is added in the container for filling water, and container can be specifically beaker, test tube etc..Container is heated, will can specifically be burnt
Cup is placed into heating plate.Meanwhile, stirred using bar magnet, the metal ion in adsorbent solution.Sequentially add 20-40 parts of breasts
Acid, 20-40 parts of phenol crystallizations, 0.05-0.1 parts of aniline blues；Persistently solution is heated and solution is stirred and evenly mixed using bar magnet,
Until temperature reaches 70-90 DEG C, stop heating, lactophenol cotton blue dyeing liquor is made.Wherein it is possible to add a temperature in a reservoir
Meter carrys out detection temperature.Held in container for deionized water, prevent that the metal ion in water from influenceing the Color of dyeing liquor.
Stop after heating, the lactophenol cotton blue dyeing liquor of preparation can be cooled down, be dispensed into afterwards in drop bottle, cover tightly bottle cap, room temperature
Preserve.
Embodiment 1：
A kind of lactophenol cotton blue dyeing liquor, including 10 parts of PVA, 20 parts of lactic acid, 20 parts of phenol and 0.05 part of aniline blue.
Corresponding, the preparation method of the lactophenol cotton blue dyeing liquor of the embodiment is as follows：
In the beaker for the deionized water that 10 parts of PVA powder are added to 50M, beaker is placed on the plate that can be stirred of heating, plus
Enter a bar magnet to be used for mixing；A thermometer is put into beaker and carrys out monitoring temperature；Sequentially add 20 parts of lactic acid, 20 parts of phenol knots
Brilliant and 0.05 part of aniline blue.Heat and mix solution until temperature up to 70,75,80,85 or 90 DEG C, removed from heating plate.Will
The lactophenol cotton blue dyeing liquor of preparation is dispensed into small drop bottle, covers tightly bottle cap, room temperature preservation.
Embodiment 2：
A kind of lactophenol cotton blue dyeing liquor, including 12 parts of PVA, 30 parts of lactic acid, 30 parts of phenol and 0.075 part of aniline blue.
Corresponding, the preparation method of the lactophenol cotton blue dyeing liquor of the embodiment is as follows：
In the beaker for the deionized water that 12 parts of PVA powder are added to 50M, beaker is placed on the plate that can be stirred of heating, plus
Enter a bar magnet to be used for mixing；A thermometer is put into beaker and carrys out monitoring temperature；Sequentially add 30 parts of lactic acid, 30 parts of phenol knots
Brilliant and 0.075 part of aniline blue.Heat and mix solution until temperature up to 70,75,80,85 or 90 DEG C, removed from heating plate.Will
The lactophenol cotton blue dyeing liquor of preparation is dispensed into small drop bottle, covers tightly bottle cap, room temperature preservation.
Embodiment 3：
A kind of lactophenol cotton blue dyeing liquor, including 15 parts of PVA, 40 parts of lactic acid, 40 parts of phenol and 0.1 part of aniline blue.
Corresponding, the preparation method of the lactophenol cotton blue dyeing liquor of the embodiment is as follows：
In the beaker for the deionized water that 15 parts of PVA powder are added to 50M, beaker is placed on the plate that can be stirred of heating, plus
Enter a bar magnet to be used for mixing；A thermometer is put into beaker and carrys out monitoring temperature；Sequentially add 40 parts of lactic acid, 40 parts of phenol knots
Brilliant and 0.1 part of aniline blue.Heat and mix solution until temperature up to 72,76,84,87 or 90 DEG C, removed from heating plate.Will system
Standby lactophenol cotton blue dyeing liquor is dispensed into small drop bottle, covers tightly bottle cap, room temperature preservation.
Above content is to combine specific embodiment further description made for the present invention, it is impossible to assert this hair
Bright specific implementation is confined to these explanations.For general technical staff of the technical field of the invention, do not taking off
On the premise of from present inventive concept, some simple deduction or replace can also be made.
Claims (7)
1. a kind of lactophenol cotton blue dyeing liquor, it is characterised in that including 10-15 parts of PVA, 20-40 parts of lactic acid, 20-40 parts of phenol
With 0.05-0.1 parts of aniline blues.
2. lactophenol cotton blue dyeing liquor according to claim 1, it is characterised in that：
PVA, lactic acid, the optimum quality ratio of phenol and aniline blue are 12:30:30:0.075.
3. a kind of preparation method of lactophenol cotton blue dyeing liquor, it is characterised in that comprise the following steps：
10-15 parts of PVA powder are added in the container for filling water, container is heated, meanwhile, stirred using bar magnet；
Sequentially add 20-40 parts of lactic acid, 20-40 parts of phenol crystallizations, 0.05-0.1 parts of aniline blues；
Persistently solution is heated and solution is stirred and evenly mixed using bar magnet, until temperature reaches 70-90 DEG C, stops heating, is made
Lactophenol cotton blue dyeing liquor.
4. method according to claim 3, it is characterised in that：
PVA, lactic acid, the optimum quality ratio of phenol and aniline blue are 12:30:30:0.075.
5. method according to claim 3, it is characterised in that：
Held in container for deionized water.
6. method according to claim 3, it is characterised in that：
When being heated to 80 DEG C, stop heating.
7. method according to claim 3, it is characterised in that also comprise the following steps：
The lactophenol cotton blue dyeing liquor of preparation is dispensed into drop bottle, bottle cap, room temperature preservation is covered tightly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710222262.6A CN106947799A (en) | 2017-04-07 | 2017-04-07 | A kind of lactophenol cotton blue dyeing liquor and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710222262.6A CN106947799A (en) | 2017-04-07 | 2017-04-07 | A kind of lactophenol cotton blue dyeing liquor and preparation method thereof |
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| CN106947799A true CN106947799A (en) | 2017-07-14 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109269865A (en) * | 2018-08-24 | 2019-01-25 | 中南民族大学 | For separating the coloring agent and its colouring method of midge head capsule subfossil |
| CN111157312A (en) * | 2020-01-08 | 2020-05-15 | 中华人民共和国京唐港海关 | Method for manufacturing permanent fungus slide specimen |
| CN113310767A (en) * | 2021-06-01 | 2021-08-27 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Microscopic method for pollen tube and ovule after pollination of water lily and optical microscopic tabletting manufacturing method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106323723A (en) * | 2016-11-30 | 2017-01-11 | 宁夏大学 | Double-blue staining method |
-
2017
- 2017-04-07 CN CN201710222262.6A patent/CN106947799A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106323723A (en) * | 2016-11-30 | 2017-01-11 | 宁夏大学 | Double-blue staining method |
Non-Patent Citations (1)
| Title |
|---|
| 沈定霞: "《医学重要真菌鉴定指南（第5版）》", 30 September 2016, 中华医学电子音像出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109269865A (en) * | 2018-08-24 | 2019-01-25 | 中南民族大学 | For separating the coloring agent and its colouring method of midge head capsule subfossil |
| CN111157312A (en) * | 2020-01-08 | 2020-05-15 | 中华人民共和国京唐港海关 | Method for manufacturing permanent fungus slide specimen |
| CN113310767A (en) * | 2021-06-01 | 2021-08-27 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Microscopic method for pollen tube and ovule after pollination of water lily and optical microscopic tabletting manufacturing method |
| CN113310767B (en) * | 2021-06-01 | 2024-05-03 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Microscopic method for pollen tube and ovule after pollination of water lily and optical microscopic tablet manufacturing method |
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Application publication date: 20170714 |