CN109612807A - A kind of urinary formed element dyeing liquor - Google Patents
A kind of urinary formed element dyeing liquor Download PDFInfo
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- CN109612807A CN109612807A CN201811635562.8A CN201811635562A CN109612807A CN 109612807 A CN109612807 A CN 109612807A CN 201811635562 A CN201811635562 A CN 201811635562A CN 109612807 A CN109612807 A CN 109612807A
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- 238000004043 dyeing Methods 0.000 title claims abstract description 35
- 230000002485 urinary effect Effects 0.000 title claims abstract description 26
- 239000000975 dye Substances 0.000 claims abstract description 25
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 21
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229960000907 methylthioninium chloride Drugs 0.000 claims abstract description 20
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 19
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 19
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 18
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 18
- 239000002562 thickening agent Substances 0.000 claims abstract description 17
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims abstract description 14
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000003755 preservative agent Substances 0.000 claims abstract description 14
- 230000002335 preservative effect Effects 0.000 claims abstract description 14
- 239000007853 buffer solution Substances 0.000 claims abstract description 11
- RZUBARUFLYGOGC-MTHOTQAESA-L acid fuchsin Chemical compound [Na+].[Na+].[O-]S(=O)(=O)C1=C(N)C(C)=CC(C(=C\2C=C(C(=[NH2+])C=C/2)S([O-])(=O)=O)\C=2C=C(C(N)=CC=2)S([O-])(=O)=O)=C1 RZUBARUFLYGOGC-MTHOTQAESA-L 0.000 claims abstract description 10
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 claims abstract description 10
- ZXJXZNDDNMQXFV-UHFFFAOYSA-M crystal violet Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1[C+](C=1C=CC(=CC=1)N(C)C)C1=CC=C(N(C)C)C=C1 ZXJXZNDDNMQXFV-UHFFFAOYSA-M 0.000 claims abstract description 9
- 229960001235 gentian violet Drugs 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 9
- MCPLVIGCWWTHFH-UHFFFAOYSA-L methyl blue Chemical compound [Na+].[Na+].C1=CC(S(=O)(=O)[O-])=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[NH+]C=2C=CC(=CC=2)S([O-])(=O)=O)C=2C=CC(NC=3C=CC(=CC=3)S([O-])(=O)=O)=CC=2)C=C1 MCPLVIGCWWTHFH-UHFFFAOYSA-L 0.000 claims abstract description 8
- 102000019197 Superoxide Dismutase Human genes 0.000 claims abstract description 7
- 108010012715 Superoxide dismutase Proteins 0.000 claims abstract description 7
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 5
- SJEYSFABYSGQBG-UHFFFAOYSA-M Patent blue Chemical group [Na+].C1=CC(N(CC)CC)=CC=C1C(C=1C(=CC(=CC=1)S([O-])(=O)=O)S([O-])(=O)=O)=C1C=CC(=[N+](CC)CC)C=C1 SJEYSFABYSGQBG-UHFFFAOYSA-M 0.000 claims description 4
- 239000000980 acid dye Substances 0.000 claims description 4
- 239000000981 basic dye Substances 0.000 claims description 4
- 125000005599 alkyl carboxylate group Chemical group 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 claims description 3
- -1 propylene ethyl glycol Chemical compound 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 240000004343 Indigofera suffruticosa Species 0.000 claims 1
- 210000002700 urine Anatomy 0.000 abstract description 15
- 238000007689 inspection Methods 0.000 abstract description 6
- 238000011882 arthroplasty Methods 0.000 abstract description 2
- 230000015572 biosynthetic process Effects 0.000 abstract description 2
- 238000012631 diagnostic technique Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 5
- 238000003745 diagnosis Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 244000061458 Solanum melongena Species 0.000 description 4
- 210000000805 cytoplasm Anatomy 0.000 description 4
- 238000002425 crystallisation Methods 0.000 description 3
- 230000008025 crystallization Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 210000002919 epithelial cell Anatomy 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 230000001376 precipitating effect Effects 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000003381 solubilizing effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical class [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- 244000283207 Indigofera tinctoria Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229940069428 antacid Drugs 0.000 description 1
- 239000003159 antacid agent Substances 0.000 description 1
- 230000001458 anti-acid effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000000232 gallbladder Anatomy 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000002715 modification method Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of urinary formed element dyeing liquors, are related to vitro diagnostic techniques field.The present invention includes that the raw material of following parts by weight is made: dyestuff 0.9~1.1；Buffer system 98~102；Antioxidant 0.09~0.11；Preservative 0.04~0.06；Protein thickeners 0.15~0.25；Dyestuff is composed of the following components in parts by weight: Yihong 12~15, acid fuchsin 11~13, and Congo red 3~5, methyl blue 6~9, gentian violet 6.3~7.5, methylene blue 2.5~3.1, methylene blue 4~6, sarranine 10~12；Antioxidant is composed of the following components in parts by weight: Sodium azide 12~15, superoxide dismutase 7.5~8.6；Protein thickeners are composed of the following components in parts by weight: dimethyl sulfoxide 6~7.5, glycerol 26~31；Urinary formed element dyeing liquor of the present invention is clear through dyeing rear backdrop, and formation in urine and background contrast are distinct, convenient for the inspection of cervical arthroplasty and corresponding instrument, extends the holding time.
Description
Technical field
The present invention relates to vitro diagnostic techniques field, specially a kind of urinary formed element dyeing liquor.
Background technique
Urinary formed element inspection can make up missing inspection or mistaken diagnosis caused by urine physico-chemical examination deficiency face.Urine to be formed
Sorting looks into also referred to as uropsammus examination, variation of this inspection technique to each position of urinary system is understood, urinary system correlation disease
Level diagnosis, antidiastole and the Index for diagnosis etc. of disease have important clinical significance, therefore urinary formed element inspection is referred to as " body
Outer Renal biospy ".Though common Microscopical Method For Detection can be appreciated that the morphological change of urinary formed element, staining technique then can will be each in urine
Kind visible component dyes different colors, more clearly shows the fine structure of certain cells, makes cell in urine, cast etc.
It is easily distinguishable, substantially increase the accurate judgement to visible component in urine.Urinary formed element staining technique dyes, again from single
Since closing dyeing release, various modification methods are used for clinic in succession, these staining techniques verify the diagnosis of urinary system related disease
With antidiastole, Index for diagnosis and drug therapy monitoring etc. play important function.
Stemheimer-Malbin (S-M) decoration method and Stemheimer decoration method at this stage is formed to urine
Divide most classic colouring method, but both dye liquors holding time after preparation is shorter, is also easy to produce more precipitating after dyeing,
It is not very greatly, to be easy to interfere reviewer and judge result, therefore existing dyeing liquor to visible component identifications certain in urine effect
Holding time it is short and be unfavorable for identify be those skilled in the art's technical issues that need to address.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of urinary formed element dyeing liquors, by dyeing liquor
Protein solubilizing reagent, which is added, avoids dyeing liquor from generating precipitating, keeps dyeing rear backdrop clear, urinary formed element is fresh with background contrast
It is bright, it solves the problems, such as that the existing dyeing liquor holding time is short and is unfavorable for identifying.
In order to achieve the above object, the present invention is achieved by the following technical programs: a kind of urinary formed element dyeing liquor,
Raw material including following parts by weight is made:
Dyestuff 0.9~1.1；
Buffer system 98~102；
Antioxidant 0.09~0.11；
Preservative 0.04~0.06；
Protein thickeners 0.15~0.25；
Wherein, contain a large amount of antacid composition and alkali resistant ingredient in the buffer system simultaneously, pass through weak acid dissociation equilibrium
Movement consume external a small amount of strong acid, highly basic to reach, so that Solution H+ion or OH- ion concentration is not had significant change, protect
Acid dyes and basic dye are demonstrate,proved within the scope of preferably work PH, PH range is 5.3~7.5, will not be caused to cellular morphology
Too big injury.
The dyestuff is composed of the following components in parts by weight: Yihong 12~15, acid fuchsin 11~13, and Congo red 3~5,
Methyl blue 6~9, gentian violet 6.3~7.5, methylene blue 2.5~3.1, methylene blue 4~6, sarranine 10~12；
The antioxidant is composed of the following components in parts by weight: Sodium azide 12~15, and superoxide dismutase 7.5~
8.6；Ratio of the antioxidant in dyeing liquor is 5/10000ths, and dyeing liquor can be prevented to be oxidized and stablize cell
Form；
The protein thickeners are composed of the following components in parts by weight: dimethyl sulfoxide 6~7.5, glycerol 26~31；
Ratio of the protein solubilizing reagent in dyeing liquor is 2/1000ths, avoids dyeing liquor from generating precipitating, keeps dyeing background clear.
Further, the raw material including following parts by weight is made:
Dyestuff 0.95~1.085；
Buffer system 98.3~101.5；
Antioxidant 0.091~0.105；
Preservative 0.041~0.059；
Protein thickeners 0.155~0.245.
Further, described Yihong, acid fuchsin, Congo red and methyl blue are acid dyes, the gentian violet, methylene
Blue, methylene blue and sarranine are basic dye；Its dyeing theory is: dyestuff penetrates urinary formed element and retains inside it, this process
The suction-operated of existing physics, and have the affinity interaction of chemistry, different cell component chemical property are different, to the parent of various dyestuffs
It is also different with power.Therefore, different cells can catch respective characteristic color after dyeing.
Further, the preservative is composed of the following components in parts by weight :-three -one of two-- four-isothiazolines of methyl
1.8~2.5 ,-three -one 0.5~1.2 of five-- four-isothiazolines of chloro- two-methyl, propylene ethyl glycol 5~7, alkyl carboxylate
11~16, ratio of the preservative of water 98~103 in dyeing liquor is one thousandth, makes dyeing liquor that can reach the effect of antibacterial
Fruit also can make testing result interference-free.
The invention has the following advantages:
The urinary formed element dyeing liquor, red blood cell pinkiness after dyeing；Leucocyte cytoplasm is purple, and core is in dark purple
Color, grain structure is clear and can classify；Epithelial cell cytoplasm is in aubergine or bluish violet, and core is in aubergine or bluish violet；Cast
Matrix is purple or bluish violet；Crystallization is in colourless bright crystal；It is clear to dye rear backdrop, formation in urine and background contrast
Distinctness, convenient for the inspection of cervical arthroplasty and corresponding instrument.
Certainly, it implements any of the products of the present invention and does not necessarily require achieving all the advantages described above at the same time.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's all other embodiment obtained without creative efforts belongs to the model that the present invention protects
It encloses.
Embodiment one
The present invention provides a kind of technical solution: a kind of urinary formed element dyeing liquor, the raw material system including following parts by weight
At:
Dyestuff 0.9~1.1；
Buffer system 98~102；
Antioxidant 0.09~0.11；
Preservative 0.04~0.06；
Protein thickeners 0.15~0.25；
Dyestuff is composed of the following components in parts by weight: Yihong 12~15, acid fuchsin 11~13, and Congo red 3~5, methyl
Indigo plant 6~9, gentian violet 6.3~7.5, methylene blue 2.5~3.1, methylene blue 4~6, sarranine 10~12；
Antioxidant is composed of the following components in parts by weight: Sodium azide 12~15, superoxide dismutase 7.5~8.6；
Protein thickeners are composed of the following components in parts by weight: dimethyl sulfoxide 6~7.5, glycerol 26~31.
Wherein, the raw material including following parts by weight is made:
Dyestuff 0.95~1.085；
Buffer system 98.3~101.5；
Antioxidant 0.091~0.105；
Preservative 0.041~0.059；
Protein thickeners 0.155~0.245.
Wherein, Yihong, acid fuchsin, Congo red and methyl blue be acid dyes, gentian violet, methylene blue, methylene blue and kind
Red is basic dye.
Wherein, preservative is composed of the following components in parts by weight :-three -one 1.8~2.5 of two-- four-isothiazolines of methyl,
- three -one 0.5~1.2 of five-- four-isothiazolines of chloro- two-methyl, propylene ethyl glycol 5~7, alkyl carboxylate 11~16, water
98~103.
The concrete application of the present embodiment are as follows:
Step 1: it takes urine specimen: urine specimen being stood 15~30 minutes, takes bottom urine 60ul drop in glass slide
On；
Step 2: add dye liquor: taking 60ul urinary formed element dye liquor drop on glass slide, with urine specimen according to 1:1's
Ratio mixing；
Step 3: cover plate: the covered on the sample of mixing stands 10~30 seconds；
Step 4: the slide prepared observation result: is placed in the visible component in microscopically observation urine.Through dyeing
Afterwards, the visible components such as the red blood cell in urine, leucocyte, epithelial cell, cast, crystallization are well arranged, red blood cell pinkiness；
Leucocyte cytoplasm is purple, and core is dark purple, and grain structure is clear and can classify；Epithelial cell cytoplasm is in aubergine or royal purple
Color, core are in aubergine or bluish violet；Cast matrix is purple or bluish violet；Crystallization is in colourless bright crystal.And dying-stable
Property is good, and it is almost unchanged to dye 1 hour inner cell form.
Embodiment two
The present invention also provides a kind of technical solutions: a kind of urinary formed element dyeing liquor, the raw material including following parts by weight
It is made:
Dyestuff 0.92~1.08；
Buffer system 98.5~101；
Antioxidant 0.091~0.103；
Preservative 0.041~0.058；
Protein thickeners 0.16~0.24；
Dyestuff is composed of the following components in parts by weight: Yihong 12.1~14.9, and acid fuchsin 11.3~12.7 is Congo red
3.3~4.6, methyl blue 6.5~8.7, gentian violet 6.5~7.4, methylene blue 2.67~3.04, methylene blue 4.5~5.7, sarranine
10.3~11.6；
Antioxidant is composed of the following components in parts by weight: Sodium azide 12.5~14.8, and superoxide dismutase 7.65~
8.35；
Protein thickeners are composed of the following components in parts by weight: dimethyl sulfoxide 6.2~7.43, and glycerol 27.1~
30.8。
Rest part is consistent with embodiment one.
Embodiment three
The present invention also provides a kind of technical solutions: a kind of urinary formed element dyeing liquor, the raw material including following parts by weight
It is made:
Dyestuff 0.93~1.05；
Buffer system 98.8~100.4；
Antioxidant 0.093~0.102；
Preservative 0.042~0.55；
Protein thickeners 0.17~0.21；
Dyestuff is composed of the following components in parts by weight: Yihong 12.3~14.4, and acid fuchsin 11.5~12.2 is Congo red
3.4~4, methyl blue 6.6~8.6, gentian violet 6.6~7.2, methylene blue 2.69~3, methylene blue 4.8~5.5, sarranine 10.9~
11.2；
Antioxidant is composed of the following components in parts by weight: Sodium azide 12.9~14.3, and superoxide dismutase 7.69~
8.19；
Protein thickeners are composed of the following components in parts by weight: dimethyl sulfoxide 6.35~7.33, and glycerol 28.1~
30。
Example IV
Invention also provides a kind of technical solution: a kind of urinary formed element dyeing liquor, the raw material system including following parts by weight
At:
Dyestuff 1；
Buffer system 100；
Antioxidant 0.1；
Preservative 0.05；
Protein thickeners 0.2；
Dyestuff is composed of the following components in parts by weight: Yihong 13.2, acid fuchsin 11.9, and Congo red 3.6, methyl blue 7, dragon
Gallbladder purple 7, methylene blue 2.81, methylene blue 5.2, sarranine 11；
Antioxidant is composed of the following components in parts by weight: Sodium azide 13.5, superoxide dismutase 7.9；
Protein thickeners are composed of the following components in parts by weight: dimethyl sulfoxide 6.8, glycerol 29.3.
Rest part is consistent with embodiment one.
In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means
Particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one implementation of the invention
In example or example.In the present specification, schematic expression of the above terms may not refer to the same embodiment or example.
Moreover, particular features, structures, materials, or characteristics described can be in any one or more of the embodiments or examples to close
Suitable mode combines.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment
All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification,
It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to better explain the present invention
Principle and practical application, so that skilled artisan be enable to better understand and utilize the present invention.The present invention is only
It is limited by claims and its full scope and equivalent.
Claims (4)
1. a kind of urinary formed element dyeing liquor, it is characterised in that the raw material including following parts by weight is made:
Dyestuff 0.9~1.1；
Buffer system 98~102；
Antioxidant 0.09~0.11；
Preservative 0.04~0.06；
Protein thickeners 0.15~0.25；
The dyestuff is composed of the following components in parts by weight: Yihong 12~15, acid fuchsin 11~13, and Congo red 3~5, methyl
Indigo plant 6~9, gentian violet 6.3~7.5, methylene blue 2.5~3.1, methylene blue 4~6, sarranine 10~12；
The antioxidant is composed of the following components in parts by weight: Sodium azide 12~15, superoxide dismutase 7.5~8.6；
The protein thickeners are composed of the following components in parts by weight: dimethyl sulfoxide 6~7.5, glycerol 26~31.
2. a kind of urinary formed element dyeing liquor according to claim 1, which is characterized in that the original including following parts by weight
Material is made:
Dyestuff 0.95~1.085；
Buffer system 98.3~101.5；
Antioxidant 0.091~0.105；
Preservative 0.041~0.059；
Protein thickeners 0.155~0.245.
3. a kind of urinary formed element dyeing liquor according to claim 1, which is characterized in that described Yihong, acid fuchsin,
Congo red and methyl blue is acid dyes, and the gentian violet, methylene blue, methylene blue and sarranine are basic dye.
4. a kind of urinary formed element dyeing liquor according to claim 1, which is characterized in that the preservative is by following heavy
Amount part at being grouped as :-three -one 1.8~2.5 of two-- four-isothiazolines of methyl, five-- four-isothiazolines of chloro- two-methyl-
Three -one 0.5~1.2, propylene ethyl glycol 5~7, alkyl carboxylate 11~16, water 98~103.
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| CN201811635562.8A CN109612807A (en) | 2018-12-29 | 2018-12-29 | A kind of urinary formed element dyeing liquor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811635562.8A CN109612807A (en) | 2018-12-29 | 2018-12-29 | A kind of urinary formed element dyeing liquor |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112213172A (en) * | 2020-09-16 | 2021-01-12 | 迪瑞医疗科技股份有限公司 | Stable vaginal secretion visible component staining solution and preparation method thereof |
| WO2021036368A1 (en) * | 2019-08-28 | 2021-03-04 | 昆氏(深圳)生物科技有限公司 | Epidermis fungus sampling and staining method |
| WO2021240914A1 (en) * | 2020-05-26 | 2021-12-02 | 東洋紡株式会社 | Urine sediment staining liquid |
| CN114264531A (en) * | 2021-12-23 | 2022-04-01 | 天津华林医疗科技有限公司 | Super-living body S staining solution |
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| CN101183054A (en) * | 2007-12-14 | 2008-05-21 | 暨南大学 | Urine process method |
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