CN105628478B - A kind of section washing apparatus - Google Patents

A kind of section washing apparatus Download PDF

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Publication number
CN105628478B
CN105628478B CN201610047912.3A CN201610047912A CN105628478B CN 105628478 B CN105628478 B CN 105628478B CN 201610047912 A CN201610047912 A CN 201610047912A CN 105628478 B CN105628478 B CN 105628478B
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CN
China
Prior art keywords
main chamber
plate
side room
screen pack
left plate
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Expired - Fee Related
Application number
CN201610047912.3A
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Chinese (zh)
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CN105628478A (en
Inventor
曾斌
其他发明人请求不公开姓名
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Nanchang Demanduo Technology Co Ltd
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Nanchang Demanduo Technology Co Ltd
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Priority to CN201610047912.3A priority Critical patent/CN105628478B/en
Publication of CN105628478A publication Critical patent/CN105628478A/en
Application granted granted Critical
Publication of CN105628478B publication Critical patent/CN105628478B/en
Expired - Fee Related legal-status Critical Current
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention provides a kind of section washing apparatus.It is made up of main chamber, side room, fluorescent tube, drift piece water heating pipe, water pump, word seam outlet pipe, screen pack heating ring, screen pack, temperature sensor, control panel etc..The temperature distribution gradient of drift piece water is realized, meets the requirement of the drift piece water of different tissues section needs different temperatures;Light source irradiation histotomy from bottom to top is provided, and the position of light source can be adjusted according to the position of histotomy, whether the gauffer for being easy to tissues observed to cut into slices is completely eliminated, so as to accurately hold the opportunity for picking up histotomy;It is easy to remove the histotomy for remaining in drift piece water surface.

Description

A kind of section washing apparatus
Technical field
The present invention relates to Bioexperiment apparatus field, particularly relates to a kind of section washing apparatus.
Background technology
Paraffin section be histopathological examination use a kind of basic skills, intend check tissue sample need to through dehydration, After FFPE, section, a series of drift programs such as piece and dyeing, it can just complete.Drift piece is the key for making paraffin section One of step, it is that will to be cut into thickness be that histotomy relaxes after 4-6 μm of tissue slice is placed on the water surface certain time of proper temperature Open up so as to eliminate gauffer.
During piece is floated, the temperature of drift piece water is whether the gauffer for influenceing histotomy can succeed one eliminated mainly Factor, most currently used drift piece coolant-temperature gage is 40 DEG C, and this is temperature needed for most of tissues, but is not suitable for all groups Knit.The water temperature that some tissues need is slightly above 40 DEG C(Such as containing the more tissue of collagenous fibres), and separately have then omiting for some needs Less than 40 DEG C(Such as fatty more tissue).If histotomy is placed higher than to the drift piece water of its optimum temperature, histotomy It will quickly crush or even melt;If histotomy to be positioned below to the drift piece water of its optimum temperature, histotomy can not relax Open up and gauffer be present.Histotomy is either broken still to have gauffer, will all influence observation of the Pathology Doctors ' to histotomy, And then influence the correctness of pathological diagnosis.Although presently commercially available section washing apparatus drift piece coolant-temperature gage is adjustable, single temperature is used, Because temperature needed for different tissues section is different, therefore, it is necessary to research and develop new section washing apparatus.
Another principal element for influenceing to eliminate histotomy gauffer is that histotomy is placed in the time length for floating piece water surface It is short.Even if histotomy is positioned over the drift piece water surface of optimum temperature, if overlong time, histotomy will be caused broken or even melted Change, if the time is too short, histotomy can not unfold and gauffer be present.This time is observed by the naked eye by operator to sentence It is disconnected, it is necessary to as early as possible be picked up histotomy with slide after finding that histotomy gauffer disappears, therefore, macroscopic standard True property plays an important role.Presently commercially available section washing apparatus are not provided with light source in drift piece water, and light source needed for tissues observed section uses room Intraoral illumination, this is unfavorable for whether tissues observed section gauffer disappears so trickle change.
After the histotomy drift piece that a sample is completed in processing, the histotomy for floating piece water surface residual need to be removed Go, otherwise may pollute the histotomy of next sample.In use, the step is by hand to presently commercially available section washing apparatus Work is completed, i.e., be placed in drift piece water surface with paper is dragged to the other end from one end, residual tissue section is taken away, so operation makes work Efficiency reduces.
The content of the invention
It is an object of the invention to provide a kind of section washing apparatus, its technical problem to be solved is:Traditional section washing apparatus without Method provides the drift piece water of various temperature, can not provide the light source for being easy to tissues observed section gauffer, can not remove drift piece water automatically Remained on surface histotomy.
In order to solve the above technical problems, the present invention provides a kind of section washing apparatus, it includes:Main chamber's left plate, main chamber's right panel, main chamber Foreboard, main chamber's back plate, main chamber's lower plate, glass plate, left support frame, right support frame, guide rail, mobile vehicle, fluorescent tube, guide rail installation spiral shell Nail, drift piece water heating pipe, word seam outlet pipe, tap, side room left plate, side room foreboard, side room back plate and side room lower plate, water pump, Water pump mounting screw, filtered fluid support plate, screen pack heating ring, screen pack, support, drawer, left temperature sensor, middle TEMP Device, right temperature sensor, side room temperature sensor, drift piece water, control panel.
Main chamber's left plate, main chamber's right panel, main chamber's foreboard, main chamber's back plate, main chamber's lower plate surround main chamber, i.e., one be open to On rectangular groove.Two guide rails being parallel to each other are arranged on master with direction from left to right with guide rail mounting screw Perpendicular with main chamber's left plate and main chamber's right panel in the lower plate of room, the mobile vehicle perpendicular to the direction of guide rail to be arranged on guide rail On, and a fluorescent tube is installed on mobile vehicle, in the control of the outwardly directed adjustable lighting tube brightness of part installation of mobile vehicle System switch.Left support frame and each two of right support frame are installed in main chamber's left plate, the lower inside surface of main chamber's right panel respectively, for propping up Support one piece of water white transparency glass plate, make between glass plate and main chamber's lower plate formed a cell, the cell be large enough to hold guide rail, Mobile vehicle and fluorescent tube, and guarantee fluorescent tube and smoothly move left and right.Glass plate forms the groove of an opening upwards, is used for Contain drift piece water.Bar shaped windowing is arranged at main chamber's foreboard bottom, makes mobile vehicle one end protruding and forms handle, in order to Hand propelled mobile vehicle and the power switch of installation fluorescent tube and water pump.The shape and size of the glass plate are left with the main chamber The lacuna bottom that plate, main chamber's right panel, main chamber's foreboard and main chamber's back plate surround matches, and glass plate is arranged on left support frame and right branch On the platform that support is formed.The glass plate has a round close to main chamber left plate side, floats piece water heating pipe for installing.Institute State drift piece water heating pipe and be arranged on glass plate at main chamber's left plate, be immersed in drift piece water, and prevented by sealing ring Float the leakage of piece water.
The side room left plate, side room foreboard, side room back plate and side room lower plate are arranged on the lateral surface shape on main chamber left plate top Into side room, i.e., the groove of one opening upwards small compared with main chamber.The water pump is arranged in the lower plate of side room by water pump mounting screw, Water circulation hydrodynamics can be provided.The top installation filtered fluid support plate of water pump, the filtered fluid support plate are used to carry by filter screen filtration Drift piece water afterwards.The circulation feed tube is connected with pump intake, will can filtered to pass through filtered fluid support plate from the top down Drift piece water above liquid support plate is introduced into water pump.The outlet pipe is connected with the delivery port of water pump, passes a side room left side to the left Plate, pass through side room back plate rear and main chamber back plate rear, main chamber right panel top is reached, be connected with word seam outlet pipe.It is described One word seam outlet pipe is equal to the width of glass plate, closed at both ends, a diameter of 10-20mm, the side installed to the left for a root long degree It is 1-3mm linear gaps to have a width, and gap length is equal to the length of word seam outlet pipe, installed in main chamber right panel top Medial surface, the residual tissue section for floating piece water surface can be blown on screen pack to the left.The outlet pipe and word seam outlet pipe Middle part be mutually connected.
The filtered fluid support plate upper central, a support is installed, drawer is rack-mount, and drawer is located in screen pack The lower section of centre, between screen pack and drawer, installation screen pack heating ring, after screen pack heating ring is powered, add screen pack Heat, the wax on screen pack will melt and drips in drawer.The screen pack heating ring is fixedly mounted on the medial surface of side room left plate, With drawer corresponding installation up and down.The screen pack is fixedly mounted in the default groove of side room left plate and main chamber's left plate opposite face institute, mistake The mediad lower recess of filter screen.
The upper limb installation of the side room left plate, side room foreboard, side room back plate and main chamber's foreboard, main chamber's back plate, main chamber's right panel Highly identical, the height of main chamber's left plate upper limb is identical with the chinky altitude of word seam outlet pipe, the height ratio of main chamber's left plate upper limb The low 2-5cm of upper limb of main chamber's right panel.The side room left plate, side room foreboard, side room back plate, main chamber's left plate are identical with main chamber's right panel, Main chamber's foreboard and the width of main chamber's back plate it is equal and more than main chamber's left plate width, it is rectangle to make formed groove, excellent Select the width in main chamber's foreboard and main chamber's back plate equal and for twice of main chamber's left plate width.
In the top of filtered fluid support plate, main chamber's left plate has a circular outlet pass, a diameter of 1- of the outlet pass 3cm, the lower edge of outlet pass is concordant with above filtered fluid support plate, makes main chamber with side room in this Communicating.The control panel is read Left temperature sensor, middle temperature sensor, right temperature sensor and the respective temperature value of side room temperature sensor, and can control Filter screen heating ring, the corresponding heating process of drift piece water heating pipe.The control of the power switch and regulation lighting tube brightness of the water pump is opened Close on the handle of mobile vehicle, word seam outlet pipe water spray can be made when the power switch of water pump is opened, in regulation lamp Bright dark change can occur for lighting tube brightness during the controlling switch rotation of pipe brightness.
When intending starting working, first cutout cock, poured into after being filled with water with container in the main chamber of glass plate, until horizontal plane Rise to a word seam outlet pipe gap it is concordant untill, make drift piece water heating pipe be powered, when left temperature sensor detects main chamber When the drift piece coolant-temperature gage of left part rises to design temperature, drift piece water heating pipe is stopped, and is detected in left temperature sensor Restart work when being less than design temperature to drift piece coolant-temperature gage, maintain drift piece coolant-temperature gage in setting range.Middle temperature sensor, The effect of right temperature sensor is only to show the temperature of position drift piece water, due to only heating the drift piece water of left part, floats piece The temperature of water is right low left high, forms thermograde.When the temperature of display drift piece water is required temperature, operator cuts tissue Piece is put into the surface of proper temperature drift piece water, connects tube power and fluorescent tube is moved to the lower section of histotomy position, After adjusting lighting tube brightness to appropriate brightness, when the gauffer for observing histotomy is wholly absent, immediately with slide by tissue Section picks up.If the drift piece coolant-temperature gage at position is not suitable for where operator has found histotomy, can be moved to by histotomy The more suitable drift piece water surface of temperature, and fluorescent tube is adjusted to corresponding position.After the drift piece of a tissue is completed, water is connected The power supply of pump, water pump will promote water circulation, water is sprayed from the gap of word seam outlet pipe, will remain in drift piece water surface Histotomy is blown on screen pack.After the residual tissue section of drift piece water surface is removed, it is ready for being put into from next piece The histotomy of sample.After end-of-job, the power supply of drift piece water heating pipe is disconnected, drift piece water is discharged from tap, then connects Screen pack heats the power supply of ring, the temperature of screen pack is increased to wax fusing, the wax of fusing carries histotomy and instilled in drawer. Drawer need to be emptied periodically.
Brief description of the drawings
Fig. 1 is the front cross-sectional structure chart of the present invention;
Fig. 2 is the guide rail and mobile vehicle installation diagram in the present invention;
Fig. 3 is that the word in the present invention stitches outlet pipe shape graph;
Fig. 4 is drift piece water heating pipe layout viewing in the present invention;
Fig. 5 is the left side view of the present invention;
Fig. 6 is the main chamber's foreboard and side room foreboard figure with bar shaped windowing of the present invention;
Wherein:1st, side room left plate;2nd, screen pack heating ring;3rd, screen pack;4th, main chamber's left plate;5th, piece water heating pipe is floated;6、 Left temperature sensor;7th, piece water is floated;8th, middle temperature sensor;9th, right temperature sensor;10th, word seam outlet pipe;11st, main chamber is right Plate;12nd, control panel;13rd, special seal gum;14th, glass plate;15th, right support frame;16th, guide rail;17th, main chamber's lower plate;18th, move Dynamic load body;19th, fluorescent tube;20th, guide rail mounting screw;21st, left support frame;22nd, it is leading;23rd, sealing ring;24th, side room lower plate;25、 Water pump;26;27th, water pump mounting screw;28th, filtered fluid support plate;29th, feed tube is circulated;30th, outlet pass;31st, support;32nd, side room Temperature sensor;33rd, drawer;34th, outlet pipe;35th, side room foreboard;36th, main chamber's foreboard.
Embodiment
Technical scheme is further elaborated with specific embodiment below in conjunction with the accompanying drawings.
Embodiment 1
Main chamber's left plate 4, main chamber's right panel 11, main chamber's foreboard, main chamber's back plate, main chamber's lower plate 17 surround an opening upwards Groove, inside dimension is 40cm × 20cm × 30cm, and the upper limb of main chamber's left plate 4 is lower 3cm than the upper limb of main chamber right panel 11.Use guide rail The guide rail 16 that described two are parallel to each other by mounting screw 20 is arranged in main chamber's lower plate 17 with direction from left to right, with main chamber Left plate 4 and main chamber's right panel 11 are perpendicular, the mobile vehicle 18 with perpendicular to the direction of guide rail 16 installation on track 16, and A piece LED lamp tube 19 is installed on mobile vehicle 18.The bottom of main chamber's foreboard 36 windowing, in order to mobile vehicle 18 handle to Overhang, the controlling switch of the adjustable brightness of fluorescent tube 19 is installed on the handle of mobile vehicle 18.Respectively in main chamber's left plate 4, master The lower inside surface installation left support frame 21 and right support frame 15 each two of room right panel 11, for support a block size be 40cm × The glass plate 14 of 20cm water white transparency, make to form a cell between glass plate 14 and main chamber's lower plate 17, the cell holds enough Receive guide rail 16, mobile vehicle 18 and fluorescent tube 19, and guarantee fluorescent tube 19 and smoothly move left and right.Glass plate 14 and surrounding structure Gap special seal gum 13 is closed, and glass plate contains drift piece water 7.The glass plate 14 has at left side edge 5cm One round, for installing drift piece water heating pipe 5.The drift piece water heating pipe 5 is immersed in drift piece water 7, and passes through sealing ring 23 Drift piece water 7 is prevented to leak.
The side room left plate 1, side room foreboard 35, side room back plate and side room lower plate 24 are arranged on the outer of the top of main chamber's left plate 4 Side, forms the groove that an opening upwards inside dimension is 10cm × 20cm × 15cm, and the water pump 25 installs spiral shell by water pump Nail 27 is arranged in side room lower plate 24.The top installation dimension of water pump 25 is 10cm × 20cm filtered fluid support plate 28.It is described to follow Ring feed tube 29 is arranged on filtered fluid support plate 28, and the drift piece water that filtered fluid support plate 28 carries can be introduced into water pump 25.From water The water that pump 25 is drawn enters outlet pipe 34, and the outlet pipe 34 passes side room left plate 1 to the left, and pass through side room back plate rear and master Room back plate rear, the top of main chamber's right panel 11 is reached, be connected with word seam outlet pipe 10.One word stitches the length of outlet pipe 10 20cm, closed at both ends, there are 2mm × 200mm linear gaps a diameter of 15mm, the side installed to the left, installed in master The upper inner identity distance of room right panel 11 from upper limb 3cm at, middle part is connected with the phase of outlet pipe 34.
The upper central of filtered fluid support plate 28, a support 31 is installed, drawer 33 is arranged on support 31,33, drawer In the lower section in the center of screen pack 3, between screen pack 3 and drawer 33, installation screen pack heating ring 2.The screen pack heats ring 2 The medial surface of side room left plate 1 is fixedly mounted on, with drawer corresponding installation about 33.The screen pack 3 is fixedly mounted on a side room left side In the default groove of plate 1 and the opposite face of main chamber's left plate 4 institute, the mediad lower recess of screen pack 3.
In the top of filtered fluid support plate 28, main chamber's left plate 4 has an a diameter of 2cm outlet pass 30, the lower edge of outlet pass 30 It is concordant above with filtered fluid support plate 28.The control panel 12 reads left temperature sensor 6, middle temperature sensor 8, right temperature and passed 32 respective temperature value of sensor 9 and side room temperature sensor, and controllable screen pack heating ring 2, drift piece water heating pipe 5 accordingly add Thermal process.The power switch of the water pump 25 is arranged on the handle of mobile vehicle 18.

Claims (7)

  1. A kind of 1. section washing apparatus, it is characterised in that:
    Main chamber's left plate, main chamber's right panel, main chamber's foreboard, main chamber's back plate, main chamber's lower plate surround the groove of an opening upwards, and guide rail is pacified In main chamber's lower plate, mobile vehicle is arranged on guide rail, and a fluorescent tube is installed on mobile vehicle, outside in mobile vehicle The controlling switch of fluorescent tube and water pump is installed, glass plate is arranged on main chamber's left plate, the lower inside of main chamber's right panel on the handle of stretching In the left support frame and right support frame in face, drift piece water heating pipe is arranged on glass plate at main chamber's left plate, and is immersed in Float in piece water;
    Side room left plate, side room foreboard, side room back plate and side room lower plate are arranged on the lateral surface on main chamber left plate top, form one and open Mouthful upward groove, the water pump are arranged in the lower plate of side room, the top installation filtered fluid support plate of water pump, circulation feed tube on to Under through filtered fluid support plate and connect pump intake, outlet pipe connects with pump outlet, passes side room left plate to the left, OK Through side room back plate rear and main chamber back plate rear, main chamber right panel top is reached, is connected with word seam outlet pipe;The filtered fluid Support plate upper central, a support is installed, drawer is rack-mount, and drawer is located at the lower section in screen pack center, in screen pack Between drawer, installation screen pack heating ring, the screen pack heating ring is fixedly mounted on the medial surface of side room left plate, with drawer Installation is corresponded to up and down, and the screen pack is fixedly mounted in the default groove of side room left plate and main chamber's left plate opposite face institute;In filtered fluid The top of support plate, main chamber's left plate have an outlet pass;Control panel reads left temperature sensor, middle temperature sensor, right temperature Sensor and the respective temperature value of side room temperature sensor, and control screen pack heating ring, drift piece water heating pipe accordingly heated Journey.
  2. A kind of 2. section washing apparatus according to claim 1, it is characterised in that:The side room left plate, side room foreboard, side room back plate It is identical with the upper limb setting height(from bottom) of main chamber's foreboard, main chamber's back plate, main chamber's right panel, height and the word seam water outlet of main chamber's left plate upper limb The chinky altitude of pipe is identical, and the height of main chamber's left plate upper limb is lower 2-5cm than the upper limb of main chamber right panel.
  3. A kind of 3. section washing apparatus according to claim 1, it is characterised in that:The glass plate water white transparency, top carrying drift Piece water, lower section installation fluorescent tube.
  4. A kind of 4. section washing apparatus according to claim 1, it is characterised in that:The drift piece water heating pipe, installed in glass plate Top is close to the position of a side wall.
  5. A kind of 5. section washing apparatus according to claim 1, it is characterised in that:The fluorescent tube, on mobile vehicle, position It is adjustable.
  6. A kind of 6. section washing apparatus according to claim 1, it is characterised in that:The length of the one word seam outlet pipe is equal to glass The width of plate, closed at both ends, it is 1-3mm linear gaps that, which there is a width side installed to the left, and gap length is equal to a word The length of outlet pipe is stitched, installed in main chamber right panel upper inner face.
  7. A kind of 7. section washing apparatus according to claim 1, it is characterised in that:The screen pack mediad lower recess, lower section peace Fill screen pack heating ring and drawer.
CN201610047912.3A 2016-01-26 2016-01-26 A kind of section washing apparatus Expired - Fee Related CN105628478B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610047912.3A CN105628478B (en) 2016-01-26 2016-01-26 A kind of section washing apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610047912.3A CN105628478B (en) 2016-01-26 2016-01-26 A kind of section washing apparatus

Publications (2)

Publication Number Publication Date
CN105628478A CN105628478A (en) 2016-06-01
CN105628478B true CN105628478B (en) 2018-03-20

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106979878B (en) * 2017-05-09 2023-12-15 贵阳德漫多医疗科技有限公司 Histopathological specimen processing system

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2526823Y (en) * 2002-03-20 2002-12-18 汪洋 Device for treating biological tissue
CN201716223U (en) * 2010-06-29 2011-01-19 常州天力兰宝科技有限公司 Water bath-slide drier shell
CN202903570U (en) * 2012-11-09 2013-04-24 广州维格斯生物科技有限公司 Section bleaching instrument
CN204027903U (en) * 2014-09-01 2014-12-17 杨翰潮 A kind of paraffin section floating box in advance

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2526823Y (en) * 2002-03-20 2002-12-18 汪洋 Device for treating biological tissue
CN201716223U (en) * 2010-06-29 2011-01-19 常州天力兰宝科技有限公司 Water bath-slide drier shell
CN202903570U (en) * 2012-11-09 2013-04-24 广州维格斯生物科技有限公司 Section bleaching instrument
CN204027903U (en) * 2014-09-01 2014-12-17 杨翰潮 A kind of paraffin section floating box in advance

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