CN105628478A - Noval slice blanching instrument - Google Patents

Noval slice blanching instrument Download PDF

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Publication number
CN105628478A
CN105628478A CN201610047912.3A CN201610047912A CN105628478A CN 105628478 A CN105628478 A CN 105628478A CN 201610047912 A CN201610047912 A CN 201610047912A CN 105628478 A CN105628478 A CN 105628478A
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CN
China
Prior art keywords
main chamber
plate
side room
water
drainage screen
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Granted
Application number
CN201610047912.3A
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Chinese (zh)
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CN105628478B (en
Inventor
曾斌
其他发明人请求不公开姓名
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Nanchang Demanduo Technology Co Ltd
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Nanchang Demanduo Technology Co Ltd
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Priority to CN201610047912.3A priority Critical patent/CN105628478B/en
Publication of CN105628478A publication Critical patent/CN105628478A/en
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Publication of CN105628478B publication Critical patent/CN105628478B/en
Expired - Fee Related legal-status Critical Current
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention provides a novel slice blanching instrument. The novel slice blanching instrument comprises a main chamber, a side chamber, a light tube, a slice blanching water heating tube, a water pump, a linear seam water outlet pipe, a filter net heating ring, a filter net, a temperature sensor and a control panel. The temperature of slice blanching water can be distributed in a gradient manner, and thus the requirement of different tissue slices on slice blanching water with different temperatures is met; the tissue slices are irradiated by a light source from top to bottom, and the position of the light source can be regulated according to the positions of the tissue slices, so that a user can conveniently observe whether wrinkles of the tissue slices are completely eliminated so as to accurately grasp the moment for fishing the tissue slices; the tissue slices left on the slice blanching water can be cleaned easily.

Description

A kind of novel section washing apparatus
Technical field
The present invention relates to biotic experiment apparatus field, particularly relate to a kind of novel section washing apparatus.
Background technology
Paraffin section is a kind of basic skills that histopathological examination adopts, and the tissue sample intending checking after a series of programs such as dehydration, paraffin embedding, section, drift sheet and dyeing, just can need to complete. Drift sheet is one of the committed step making paraffin section, is that tissue slice is unfolded thus eliminating gauffer by after being cut into the water surface certain time that the tissue slice that thickness is 4-6 ��m is placed on proper temperature.
Drift sheet process in, drift sheet water temperature be affect the principal element whether gauffer of tissue slice can successfully eliminate, most currently used drift sheet coolant-temperature gage is 40 DEG C, this be great majority organize temperature required, but be not suitable in a organized way. The water temperature of some organization need slightly above 40 DEG C (tissues as more containing collagen fiber), separately have some needs then slightly below 40 DEG C (such as fatty more tissues). If tissue slice is placed higher than the drift sheet water of its optimum temperature, tissue slice will quickly crush and even melt; If tissue slice is positioned below the drift sheet water of its optimum temperature, tissue slice can not be unfolded and there is gauffer. No matter tissue slice is broken or there is gauffer, all will affect the Pathology Doctors ' observation to tissue slice, and then affects the correctness of pathological diagnosis. Although presently commercially available section washing apparatus drift sheet coolant-temperature gage is adjustable, but all adopts single temperature, due to the different tissues temperature required difference of section, therefore, it is necessary to research and develop novel section washing apparatus.
It is the time length that tissue slice is placed in drift sheet water surface that impact eliminates another principal element of tissue slice gauffer. Even if tissue slice is positioned over the drift sheet water surface of optimum temperature, if overlong time, causing that tissue slice is broken and even melt, if the time is too short, tissue slice can not be unfolded and there is gauffer. This time is judged by perusal by operator, it is necessary to picked up by tissue slice with microscope slide as early as possible after finding the disappearance of tissue slice gauffer, therefore, macroscopic accuracy plays an important role. Presently commercially available section washing apparatus is not provided with light source in drift sheet water, and the required light source of tissues observed section adopts room lighting, and this is unfavorable for whether tissues observed section gauffer disappears so trickle change.
After the tissue slice having processed a specimen floats sheet, the tissue slice of drift sheet water surface residual need to be removed, otherwise be likely to pollute the tissue slice of next specimen. In use, this step is by having been manually done to presently commercially available section washing apparatus, and namely paper using is placed in drift sheet water surface and is dragged to the other end from one end, residual tissue section is taken away, and so operation makes work efficiency reduce.
Summary of the invention
It is an object of the invention to provide a kind of novel section washing apparatus, its technical problem to be solved is in that: tradition section washing apparatus cannot provide the drift sheet water of various temperature, the light source being easy to tissues observed section gauffer cannot be provided, it is impossible to automatically remove drift sheet water surface residual tissue section.
For solving above-mentioned technical problem, the present invention provides a kind of novel section washing apparatus, comprising: main chamber's left plate, main chamber's right panel, main chamber's header board, main chamber's back plate, main chamber's lower plate, glass plate, left support frame, right support frame, guide rail, mobile vehicle, fluorescent tube, guide rails assembling screw, drift sheet water adds heat pipe, one word seam outlet pipe, tap, side room left plate, side room header board, side room back plate and side room lower plate, water pump, screw installed by water pump, filtrate support plate, drainage screen heating ring, drainage screen, support, drawer, left temperature sensor, middle temperature sensor, right temperature sensor, side room temperature sensor, drift sheet water, control panel.
Described main chamber left plate, main chamber's right panel, main chamber's header board, main chamber's back plate, main chamber's lower plate surround main chamber, i.e. the rectangular groove of an opening upwards. With guide rails assembling screw, described two guide rails being parallel to each other are arranged in main chamber's lower plate with direction from left to right, perpendicular with main chamber's left plate and main chamber's right panel, described mobile vehicle is arranged on guide rail with the direction being perpendicular to guide rail, and a fluorescent tube is installed on mobile vehicle, the control switch of scalable lighting tube brightness is installed in the outwardly directed part of mobile vehicle. Left support frame and each two of right support frame are installed in the lower inside surface of main chamber's left plate, main chamber's right panel respectively, for supporting one piece of water white glass plate, make one cell of formation between glass plate and main chamber's lower plate, this cell is large enough to hold guide rail, mobile vehicle and fluorescent tube, and guarantees fluorescent tube and move left and right smoothly. Glass plate forms the groove of an opening upwards, is used for containing drift sheet water. Header board bottom, described main chamber has bar shaped to window, and makes mobile vehicle one end protruding and forms handle, in order to hand propelled mobile vehicle and the on and off switch installing fluorescent tube and water pump. The lacuna bottom that the shape and size of described glass plate surround with described main chamber left plate, main chamber's right panel, main chamber's header board and main chamber's back plate matches, on the platform that glass plate is arranged on left support frame and right support frame is formed. Described glass plate has a round near left plate side, main chamber, is used for installing drift sheet water and adds heat pipe. Described drift sheet water adds heat pipe and is arranged on glass plate near left plate place of main chamber, is immersed in drift sheet water, and stops drift sheet water leakage by sealing ring.
Described side room left plate, side room header board, side room back plate and side room lower plate are arranged on the lateral surface on left plate top, main chamber and form side room, the i.e. groove of an opening upwards little compared with main chamber. Described water pump is installed screw by water pump and is arranged in the lower plate of side room, it is possible to provide Water circulation hydrodynamics. The filtrate support plate installed above of water pump, described filtrate support plate is used for the drift sheet water after carrying filtered net filtration. Drift sheet water above filtrate support plate, from the top down through filtrate support plate, to be connected with pump intake, can be introduced in water pump by described circulation feed tube. Described outlet pipe is connected with the outlet of water pump, passes side room left plate to the left, pass through back plate rear, side room and back plate rear, main chamber, arrives right panel top, main chamber, stitches outlet pipe with a word and is connected. A described word seam outlet pipe is the root length degree width equal to glass plate, closed at both ends, diameter is 10-20mm, the side installed to the left has a width to be 1-3mm linear gaps, gap length is equal to the length of a word seam outlet pipe, it is arranged on right panel upper inner face, main chamber, the residual tissue section of drift sheet water surface can be blown on drainage screen to the left. The middle part of described outlet pipe and word seam outlet pipe is connected mutually.
Described filtrate support plate upper central, one support is installed, drawer is rack-mount, drawer is positioned at the lower section of drainage screen central authorities, between drainage screen and drawer, drainage screen heating ring is installed, after drainage screen heats ring energising, making drainage screen heat, the wax on drainage screen is by fusing and drips in drawer. Described drainage screen heating ring is fixedly mounted on the medial surface of side room left plate, with drawer corresponding installation up and down. Described drainage screen is fixedly mounted in the default groove of side room left plate and main chamber's left plate opposite face institute, the mediad lower recess of drainage screen.
Described side room left plate, side room header board, side room back plate and main chamber's header board, main chamber's back plate, main chamber's right panel upper limb setting height(from bottom) identical, the chinky altitude of the height of main chamber's left plate upper limb and word seam outlet pipe is identical, the low 2-5cm of upper limb of the aspect ratio main chamber right panel of main chamber's left plate upper limb. Described side room left plate, side room header board, side room back plate, main chamber's left plate are identical with main chamber's right panel, the width of described main chamber header board and main chamber's back plate is equal and width more than main chamber's left plate, making the groove formed is rectangle, it is preferable that the width in main chamber's header board and main chamber's back plate is equal and be the twice of main chamber's left plate width.
Above filtrate support plate, main chamber's left plate has a circular outlet pass, and the diameter of described outlet pass is 1-3cm, and the lower edge of outlet pass is concordant with above filtrate support plate, makes main chamber and side room at this Communicating. Described control panel reads left temperature sensor, middle temperature sensor, right temperature sensor and the respective temperature value of side room temperature sensor, and drainage screen heating ring can be controlled, drift sheet water adds the corresponding heating process of heat pipe. The on and off switch of described water pump and the switch that controls of adjustment lighting tube brightness are arranged on the handle of mobile vehicle, can make a word seam outlet pipe water spray when the on and off switch of water pump is opened, when regulating the control switch rotary of lighting tube brightness can there is bright dark change in lighting tube brightness.
When intending starting working, first cutout cock, pour in the main chamber of glass plate after being filled with water with container, until horizontal plane rise to one word stitch outlet pipe gap concordant till, making drift sheet water add heat pipe energising, when the drift sheet coolant-temperature gage that left temperature sensor detects main chamber's left part rises to design temperature, drift sheet water adds heat pipe and quits work, and restart work when left temperature sensor detects drift sheet coolant-temperature gage lower than design temperature, maintain drift sheet coolant-temperature gage at set point. Middle temperature sensor, right temperature sensor effect be only show this position drift sheet water temperature, owing to only heating the drift sheet water of left part, drift sheet water temperature be right low left high, formation temperature gradient. When the temperature of display drift sheet water is temperature required, tissue slice is put into the surface of proper temperature drift sheet water by operator, connect tube power and fluorescent tube is moved to the lower section of tissue slice position, regulate lighting tube brightness to appropriate brightness, when the gauffer observing tissue slice is wholly absent, with microscope slide, tissue slice is picked up immediately. If the drift sheet coolant-temperature gage that operator finds position, tissue slice place is not suitable for, available tissue slice is moved to temperature the drift sheet water surface being more suitable for, and adjust fluorescent tube to corresponding position. After the drift sheet completing a tissue, connecting the power supply of water pump, water pump will promote water circulation, make water stitch ejection the gap of outlet pipe from a word, be blown on drainage screen by the tissue slice remaining in drift sheet water surface. After the residual tissue section removing drift sheet water surface, it is ready for putting into the tissue slice from next block specimen. After end-of-job, disconnecting drift sheet water and add the power supply of heat pipe, discharged from tap by drift sheet water, then connect the power supply of drainage screen heating ring, make the temperature of drainage screen be increased to wax fusing, the wax of fusing carries tissue slice and instills in drawer. Drawer need to regularly empty.
Accompanying drawing explanation
Fig. 1 is the front cross-sectional structure chart of the present invention;
Fig. 2 is the guide rail in the present invention and mobile vehicle installation diagram;
Fig. 3 is the word seam outlet pipe shape graph in the present invention;
Fig. 4 floats sheet water heating bank of tubes Butut in the present invention;
Fig. 5 is the left side view of the present invention;
Fig. 6 is the main chamber's header board windowed with bar shaped and the side room header board figure of the present invention;
Wherein: 1, side room left plate; 2, drainage screen heating ring; 3, drainage screen; 4, main chamber's left plate; 5, drift sheet water adds heat pipe; 6, left temperature sensor; 7, drift sheet water; 8, middle temperature sensor; 9, right temperature sensor; 10, a word seam outlet pipe; 11, main chamber's right panel; 12, control panel; 13, special seal gum; 14, glass plate; 15, right support frame; 16, guide rail; 17, main chamber's lower plate; 18, mobile vehicle; 19, fluorescent tube; 20, guide rails assembling screw; 21, left support frame; 22, tap; 23, sealing ring; 24, side room lower plate; 25, water pump; 26; 27, screw installed by water pump; 28, filtrate support plate; 29, circulation feed tube; 30, outlet pass; 31, support; 32, side room temperature sensor; 33, drawer; 34, outlet pipe; 35, side room header board; 36, main chamber's header board.
Detailed description of the invention
Below in conjunction with accompanying drawing and specific embodiment, technical scheme is further elaborated.
Embodiment 1
Described main chamber left plate 4, main chamber's right panel 11, main chamber's header board, main chamber's back plate, main chamber's lower plate 17 surround the groove of an opening upwards, and inside dimension is 40cm �� 20cm �� 30cm, the upper limb 3cm lower than the upper limb of main chamber right panel 11 of main chamber's left plate 4. With guide rails assembling screw 20, described two guide rails being parallel to each other 16 are arranged in main chamber's lower plate 17 with direction from left to right, perpendicular with main chamber's left plate 4 and main chamber's right panel 11, described mobile vehicle 18 is installed on track 16 with the direction being perpendicular to guide rail 16, and installs a LED lamp tube 19 on mobile vehicle 18. Window header board 36 bottom in described main chamber, in order to the handle of mobile vehicle 18 is protruding, installs the control switch of scalable fluorescent tube 19 brightness on the handle of mobile vehicle 18. Left support frame 21 and each two of right support frame 15 are installed in the lower inside surface of main chamber's left plate 4, main chamber's right panel 11 respectively, for supporting one piece of water white glass plate 14 being of a size of 40cm �� 20cm, make one cell of formation between glass plate 14 and main chamber's lower plate 17, this cell is large enough to hold guide rail 16, mobile vehicle 18 and fluorescent tube 19, and guarantees fluorescent tube 19 and move left and right smoothly. The gap special seal gum 13 of glass plate 14 and surrounding structure is closed, and glass plate contains drift sheet water 7. Described glass plate 14 has a round at distance left side edge 5cm place, is used for installing drift sheet water and adds heat pipe 5. Described drift sheet water adds heat pipe 5 and is immersed in drift sheet water 7, and stops drift sheet water 7 to leak by sealing ring 23.
Described side room left plate 1, side room header board 35, side room back plate and side room lower plate 24 are arranged on the lateral surface on main chamber's left plate 4 top, forming the groove that opening upwards inside dimension is 10cm �� 20cm �� 15cm, described water pump 25 is installed screw 27 by water pump and is arranged in side room lower plate 24. The filtrate support plate 28 being of a size of 10cm �� 20cm installed above of water pump 25. Described circulation feed tube 29 is arranged on filtrate support plate 28, and the drift sheet water that can be carried by filtrate support plate 28 introduces in water pump 25. The water drawn from water pump 25 enters outlet pipe 34, and described outlet pipe 34 passes side room left plate 1 to the left, pass through back plate rear, side room and back plate rear, main chamber, arrives right panel 11 top, main chamber, stitches outlet pipe 10 with a word and is connected. Described word seam outlet pipe 10 length is 20cm, and closed at both ends, diameter is 15mm, and there is the linear gaps of a 2mm �� 200mm side installed to the left, is arranged on main chamber's right panel 11 upper inner identity distance from upper limb 3cm place, and middle part is connected with described outlet pipe 34 phase.
Described filtrate support plate 28 upper central, installs a support 31, and drawer 33 is arranged on support 31, and drawer 33 is positioned at the lower section of drainage screen 3 central authorities, between drainage screen 3 and drawer 33, installs drainage screen heating ring 2. Described drainage screen heating ring 2 is fixedly mounted on the medial surface of side room left plate 1, installation corresponding to drawer about 33. Described drainage screen 3 is fixedly mounted in the default groove of side room left plate 1 and main chamber's left plate 4 opposite face institute, the mediad lower recess of drainage screen 3.
Above filtrate support plate 28, main chamber's left plate 4 has a diameter to be 2cm outlet pass 30, and the lower edge of outlet pass 30 is concordant above with filtrate support plate 28. Described control panel 12 reads left temperature sensor 6, middle temperature sensor 8, right temperature sensor 9 and the respective temperature value of side room temperature sensor 32, and drainage screen heating ring 2 can be controlled, drift sheet water adds the corresponding heating process of heat pipe 5. The on and off switch of described water pump 25 is arranged on the handle of mobile vehicle 18.

Claims (7)

1. a novel section washing apparatus, it is characterised in that:
Described main chamber left plate, main chamber's right panel, main chamber's header board, main chamber's back plate, main chamber's lower plate surround the groove of an opening upwards, by guide rails assembling in main chamber's lower plate, mobile vehicle is arranged on guide rail, and a fluorescent tube is installed on mobile vehicle, the outwardly directed handle of mobile vehicle is installed the control switch of fluorescent tube and water pump, glass plate is arranged in left support frame and the right support frame of the lower inside surface of main chamber's left plate, main chamber's right panel, described drift sheet water adds heat pipe and is arranged on glass plate near left plate place of main chamber, and is immersed in drift sheet water;
Described side room left plate, side room header board, side room back plate and side room lower plate are arranged on the lateral surface on left plate top, main chamber, form the groove of an opening upwards, described water pump is arranged in the lower plate of side room, the filtrate support plate installed above of water pump, described circulation feed tube traverse filtrate support plate from the top down also connects pump intake, described outlet pipe connects with pump outlet, pass side room left plate to the left, pass through back plate rear, side room and back plate rear, main chamber, arrive right panel top, main chamber, stitch outlet pipe with a word and be connected; Described filtrate support plate upper central, one support is installed, drawer is rack-mount, drawer is positioned at the lower section of drainage screen central authorities, between drainage screen and drawer, installing drainage screen heating ring, described drainage screen heating ring is fixedly mounted on the medial surface of side room left plate, with drawer corresponding installation up and down, described drainage screen is fixedly mounted in the default groove of side room left plate and main chamber's left plate opposite face institute; Above filtrate support plate, main chamber's left plate has an outlet pass; Described control panel reads left temperature sensor, middle temperature sensor, right temperature sensor and the respective temperature value of side room temperature sensor, and controls drainage screen heating plate, the drift sheet corresponding heating process of water heating plate.
2. the novel section washing apparatus of one according to claim 1, it is characterized in that: described side room left plate, side room header board, side room back plate and main chamber's header board, main chamber's back plate, main chamber's right panel upper limb setting height(from bottom) identical, the chinky altitude of the height of main chamber's left plate upper limb and word seam outlet pipe is identical, the low 2-5cm of upper limb of the aspect ratio main chamber right panel of main chamber's left plate upper limb.
3. the novel section washing apparatus of one according to claim 1, it is characterised in that: described glass plate water white transparency, top carrying drift sheet water, fluorescent tube mounted below.
4. the novel section washing apparatus of one according to claim 1, it is characterised in that: described drift sheet water adds heat pipe, is arranged on the glass plate position near a sidewall.
5. the novel section washing apparatus of one according to claim 1, it is characterised in that: described fluorescent tube, it is arranged on mobile vehicle, position-adjustable.
6. the novel section washing apparatus of one according to claim 1, it is characterized in that: the length of a described word seam outlet pipe is equal to the width of glass plate, closed at both ends, the side installed to the left has a width to be 1-3mm linear gaps, gap length, equal to the length of a word seam outlet pipe, is arranged on right panel upper inner face, main chamber.
7. the novel section washing apparatus of one according to claim 1, it is characterised in that: described drainage screen mediad lower recess, drainage screen mounted below heating ring and drawer.
CN201610047912.3A 2016-01-26 2016-01-26 A kind of section washing apparatus Expired - Fee Related CN105628478B (en)

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Application Number Priority Date Filing Date Title
CN201610047912.3A CN105628478B (en) 2016-01-26 2016-01-26 A kind of section washing apparatus

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CN201610047912.3A CN105628478B (en) 2016-01-26 2016-01-26 A kind of section washing apparatus

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CN105628478B CN105628478B (en) 2018-03-20

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106979878A (en) * 2017-05-09 2017-07-25 南昌德漫多科技有限公司 A kind of histopathology sample processing system
CN109030157A (en) * 2018-07-29 2018-12-18 南昌德漫多科技有限公司 A kind of drift sheet devices and its drift piece method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2526823Y (en) * 2002-03-20 2002-12-18 汪洋 Biological tissue spreading device
CN201716223U (en) * 2010-06-29 2011-01-19 常州天力兰宝科技有限公司 Water bath-slide drier shell
CN202903570U (en) * 2012-11-09 2013-04-24 广州维格斯生物科技有限公司 Section bleaching instrument
CN204027903U (en) * 2014-09-01 2014-12-17 杨翰潮 A kind of paraffin section floating box in advance

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2526823Y (en) * 2002-03-20 2002-12-18 汪洋 Biological tissue spreading device
CN201716223U (en) * 2010-06-29 2011-01-19 常州天力兰宝科技有限公司 Water bath-slide drier shell
CN202903570U (en) * 2012-11-09 2013-04-24 广州维格斯生物科技有限公司 Section bleaching instrument
CN204027903U (en) * 2014-09-01 2014-12-17 杨翰潮 A kind of paraffin section floating box in advance

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106979878A (en) * 2017-05-09 2017-07-25 南昌德漫多科技有限公司 A kind of histopathology sample processing system
CN106979878B (en) * 2017-05-09 2023-12-15 贵阳德漫多医疗科技有限公司 Histopathological specimen processing system
CN109030157A (en) * 2018-07-29 2018-12-18 南昌德漫多科技有限公司 A kind of drift sheet devices and its drift piece method

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